Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,661,763 C→A 100% P353P (CCG→CCT)  mlc ← glucosamine anaerobic growth regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,661,7630CA99.5% 701.8 / ‑4.6 213P353P (CCG→CCT) mlcglucosamine anaerobic growth regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (113/99);  total (113/100)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 213 total aligned reads displayed.

ACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAA  >  CP009273/1661616‑1661909
                                                                                                                                                   |                                                                                                                                                  
aCGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCGCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAgg                                                                                                                                                  <  1:652553/150‑1 (MQ=255)
 cGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAggg                                                                                                                                                 >  2:690231/1‑150 (MQ=255)
  gAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGa                                                                                                                                                >  1:1817025/1‑150 (MQ=255)
    aTCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATTCTGTCTGAGATGACAGGGAag                                                                                                                                              <  1:4908893/150‑1 (MQ=255)
    aTCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAag                                                                                                                                              >  2:2617477/1‑150 (MQ=255)
     tCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAaga                                                                                                                                             >  2:3332358/1‑150 (MQ=255)
        acaaAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGa                                                                                                                                          >  2:4198933/1‑150 (MQ=255)
         caaAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGat                                                                                                                                         >  1:2326806/1‑150 (MQ=255)
          aaaGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGata                                                                                                                                        >  2:1812392/1‑150 (MQ=255)
           aaGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGatat                                                                                                                                       >  2:1100834/1‑150 (MQ=255)
            aGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATc                                                                                                                                      >  1:1324639/1‑150 (MQ=255)
              aaCCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTg                                                                                                                                    <  2:1427219/150‑1 (MQ=255)
              aaCCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTg                                                                                                                                    <  2:3721033/150‑1 (MQ=255)
                   ttATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCt                                                                                                                               <  1:1124922/150‑1 (MQ=255)
                   ttATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCt                                                                                                                               <  1:2503864/150‑1 (MQ=255)
                    tataCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCtt                                                                                                                              >  2:3833667/1‑150 (MQ=255)
                    tataCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCtt                                                                                                                              <  1:4934827/150‑1 (MQ=255)
                         aTCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACtt                                                                                                                         >  2:2345564/1‑150 (MQ=255)
                          tCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCGCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTa                                                                                                                        <  2:2036922/150‑1 (MQ=255)
                           cgcgTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGGGATGACAGGGAAGAGGATATCTGCCGCTTTACTTaa                                                                                                                       >  2:4026403/1‑150 (MQ=255)
                           cgcgTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTaa                                                                                                                       >  2:3517925/1‑150 (MQ=255)
                            gcgTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAAc                                                                                                                      <  2:4517725/150‑1 (MQ=255)
                               tCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGt                                                                                                                   <  1:2890013/150‑1 (MQ=255)
                               tCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGt                                                                                                                   <  2:2722521/150‑1 (MQ=255)
                                cTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTg                                                                                                                  <  1:3378311/150‑1 (MQ=255)
                                  tttACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAg                                                                                                                <  1:5118160/150‑1 (MQ=255)
                                    tACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGcc                                                                                                              >  2:4339082/1‑150 (MQ=255)
                                    tACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGcc                                                                                                              <  2:543648/150‑1 (MQ=255)
                                     aCCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCa                                                                                                             >  1:4112479/1‑150 (MQ=255)
                                      ccAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCaa                                                                                                            <  1:147183/150‑1 (MQ=255)
                                        aGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATc                                                                                                          >  1:1723474/1‑150 (MQ=255)
                                          tGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAg                                                                                                        <  1:2111535/150‑1 (MQ=255)
                                             aGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAt                                                                                                     >  2:2541347/1‑150 (MQ=255)
                                             aGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCGTTACTTAACGGTGAGCCAATCAGTAt                                                                                                     >  1:2058904/1‑150 (MQ=255)
                                               cgcCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAttt                                                                                                   <  2:647360/150‑1 (MQ=255)
                                               cgcCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAttt                                                                                                   >  1:1485766/1‑150 (MQ=255)
                                                  cTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAtttttt                                                                                                >  1:3996672/1‑150 (MQ=255)
                                                  cTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAtttttt                                                                                                <  1:4995456/150‑1 (MQ=255)
                                                         cGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGtt                                                                                         <  1:3295293/150‑1 (MQ=255)
                                                             cccTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaat                                                                                     <  1:4206763/150‑1 (MQ=255)
                                                              ccTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaata                                                                                    <  1:641914/150‑1 (MQ=255)
                                                                tGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaataaa                                                                                  >  1:3888260/1‑150 (MQ=255)
                                                                  gTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAAtt                                                                                <  2:2032842/150‑1 (MQ=255)
                                                                   ttAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTc                                                                               <  1:3027684/150‑1 (MQ=255)
                                                                     aGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCAc                                                                             >  2:3624219/1‑150 (MQ=255)
                                                                       aaaaCTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCa                                                                           >  1:3723215/1‑150 (MQ=255)
                                                                       aaaaCTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCa                                                                           <  2:1817025/150‑1 (MQ=255)
                                                                        aaaCTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAACTAAATTCACCat                                                                          >  2:2817613/1‑150 (MQ=255)
                                                                        aaaCTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCat                                                                          >  1:657533/1‑150 (MQ=255)
                                                                          aCTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCatga                                                                        <  2:2399273/150‑1 (MQ=255)
                                                                            cgactACTCTCAACGCTTATGTGCTGACTAGACGCAGGAAGGGCCTGCTGACGGATGATGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAAGTCACCatgatg                                                                      <  2:2677939/146‑1 (MQ=255)
                                                                               gTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCa                                                                   <  1:827492/150‑1 (MQ=255)
                                                                                    ctcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAAt                                                              <  2:3210409/150‑1 (MQ=255)
                                                                                       aaCGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATgcg                                                           <  1:1041544/150‑1 (MQ=255)
                                                                                        aCGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATgcgc                                                          <  2:863496/150‑1 (MQ=255)
                                                                                        aCGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATgcgc                                                          <  1:1549779/150‑1 (MQ=255)
                                                                                         cGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGcc                                                         <  2:4883222/150‑1 (MQ=255)
                                                                                           cTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCg                                                       >  2:502078/1‑150 (MQ=255)
                                                                                            tGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGa                                                      >  1:1665478/1‑150 (MQ=255)
                                                                                              aTGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACa                                                    <  1:3715412/150‑1 (MQ=255)
                                                                                                gtgCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATg                                                  <  1:1100834/150‑1 (MQ=255)
                                                                                                 tgCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATgc                                                 <  1:3618017/150‑1 (MQ=255)
                                                                                                  gCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATgcg                                                <  1:1014437/150‑1 (MQ=255)
                                                                                                   cTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATgcgc                                               >  1:1165066/1‑150 (MQ=255)
                                                                                                     gACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATgcgcgc                                             <  2:3233394/150‑1 (MQ=255)
                                                                                                        tataCGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCa                                          <  1:3227124/150‑1 (MQ=255)
                                                                                                            cGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCAcccc                                      >  1:595033/1‑150 (MQ=255)
                                                                                                            cGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCAcccc                                      >  1:3236341/1‑150 (MQ=255)
                                                                                                              cAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGTAGAGTATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCgg                                    <  1:4774075/150‑1 (MQ=255)
                                                                                                               aGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGt                                   >  1:3805052/1‑150 (MQ=255)
                                                                                                                 gAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTaa                                 <  1:4339298/150‑1 (MQ=255)
                                                                                                                  aaGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAat                                <  1:4587111/150‑1 (MQ=255)
                                                                                                                    gggCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatga                              >  1:2888393/1‑150 (MQ=255)
                                                                                                                     ggCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatgat                             >  1:2876374/1‑150 (MQ=255)
                                                                                                                      gCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatgatg                            <  2:112700/150‑1 (MQ=255)
                                                                                                                        ctgctgACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTc                          <  1:3086943/150‑1 (MQ=255)
                                                                                                                        ctgctgACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTc                          >  1:4026545/1‑150 (MQ=255)
                                                                                                                            tgACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCtttt                      <  2:1726236/150‑1 (MQ=255)
                                                                                                                            tgACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCtttt                      <  1:3018614/150‑1 (MQ=255)
                                                                                                                             gACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTg                     >  2:5097234/1‑150 (MQ=255)
                                                                                                                             gACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTg                     >  1:3410216/1‑150 (MQ=255)
                                                                                                                              aCGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGc                    >  2:3084894/1‑150 (MQ=255)
                                                                                                                                ggATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCa                  >  2:1863834/1‑150 (MQ=255)
                                                                                                                                ggATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCa                  >  1:4217695/1‑150 (MQ=255)
                                                                                                                                ggATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCa                  <  1:2226078/150‑1 (MQ=255)
                                                                                                                                  aTGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGt                >  2:4960774/1‑150 (MQ=255)
                                                                                                                                   tGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTa               <  2:3081641/150‑1 (MQ=255)
                                                                                                                                   tGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTa               >  1:871841/1‑150 (MQ=255)
                                                                                                                                    gCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAg              <  1:2581095/150‑1 (MQ=255)
                                                                                                                                    gCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAg              <  2:3996672/150‑1 (MQ=255)
                                                                                                                                    gCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAg              <  2:908861/150‑1 (MQ=255)
                                                                                                                                      tGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGAt            >  2:674779/1‑150 (MQ=255)
                                                                                                                                       gTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATc           >  2:2815753/1‑150 (MQ=255)
                                                                                                                                       gTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATc           >  2:2872852/1‑150 (MQ=255)
                                                                                                                                       gTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATc           <  2:3290443/150‑1 (MQ=255)
                                                                                                                                        tCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATcg          <  1:2787062/150‑1 (MQ=255)
                                                                                                                                          tGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATcgcc        >  2:4675337/1‑150 (MQ=255)
                                                                                                                                          tGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATcgcc        >  2:164294/1‑150 (MQ=255)
                                                                                                                                               tGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCa   <  1:3699390/150‑1 (MQ=255)
                                                                                                                                                gACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCaa  >  2:2985767/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAA  >  CP009273/1661616‑1661909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGC  >  CP009273/1661614‑1661912
                                                                                                                                                     |                                                                                                                                                     
AGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACA                                                                                                                                                       >  SRR19973063.1110898/1‑150 (MQ=60)
 GACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAG                                                                                                                                                      >  SRR19973063.1222873/1‑150 (MQ=60)
 GACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAG                                                                                                                                                      >  SRR19973063.1222641/1‑150 (MQ=60)
  ACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGG                                                                                                                                                     <  SRR19973063.3143403/150‑1 (MQ=60)
  ACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGG                                                                                                                                                     <  SRR19973063.654742/150‑1 (MQ=60)
  ACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCGCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGG                                                                                                                                                     <  SRR19973063.654467/150‑1 (MQ=60)
    GAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGA                                                                                                                                                   >  SRR19973063.1822675/1‑150 (MQ=60)
      ATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATTCTGTCTGAGATGACAGGGAAG                                                                                                                                                 <  SRR19973063.4924743/150‑1 (MQ=60)
      ATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAG                                                                                                                                                 <  SRR19973063.4924552/150‑1 (MQ=60)
           CAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGAT                                                                                                                                            >  SRR19973063.2334255/1‑150 (MQ=60)
           CAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGAT                                                                                                                                            >  SRR19973063.2333654/1‑150 (MQ=60)
              AGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATC                                                                                                                                         >  SRR19973063.1328602/1‑150 (MQ=60)
                 ACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGC                                                                                                                                      <  SRR19973063.4952068/150‑1 (MQ=60)
                     TTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCT                                                                                                                                  <  SRR19973063.1128338/150‑1 (MQ=60)
                     TTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCT                                                                                                                                  <  SRR19973063.2511850/150‑1 (MQ=60)
                      TATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTT                                                                                                                                 <  SRR19973063.4950768/150‑1 (MQ=60)
                      TATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTT                                                                                                                                 <  SRR19973063.3414631/150‑1 (MQ=60)
                            TCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTA                                                                                                                           >  SRR19973063.1023046/1‑150 (MQ=60)
                              GCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAAC                                                                                                                         >  SRR19973063.199981/1‑150 (MQ=60)
                                 TCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGT                                                                                                                      <  SRR19973063.2899218/150‑1 (MQ=60)
                                 TCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGT                                                                                                                      <  SRR19973063.2899241/150‑1 (MQ=60)
                                  CTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTG                                                                                                                     >  SRR19973063.634325/1‑150 (MQ=60)
                                  CTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTG                                                                                                                     <  SRR19973063.3389076/150‑1 (MQ=60)
                                    TTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAG                                                                                                                   <  SRR19973063.5134624/150‑1 (MQ=60)
                                     TTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGC                                                                                                                  >  SRR19973063.4310501/1‑150 (MQ=60)
                                       ACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCA                                                                                                                >  SRR19973063.4125650/1‑150 (MQ=60)
                                        CCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAA                                                                                                               <  SRR19973063.147637/150‑1 (MQ=60)
                                          AGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATC                                                                                                             >  SRR19973063.1728729/1‑150 (MQ=60)
                                          AGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATC                                                                                                             >  SRR19973063.5023166/1‑150 (MQ=60)
                                            TGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAG                                                                                                           <  SRR19973063.2118137/150‑1 (MQ=60)
                                               AGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCGTTACTTAACGGTGAGCCAATCAGTAT                                                                                                        >  SRR19973063.2065334/1‑150 (MQ=60)
                                                 CGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTT                                                                                                      >  SRR19973063.1490503/1‑150 (MQ=60)
                                                 CGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTT                                                                                                      >  SRR19973063.1490319/1‑150 (MQ=60)
                                                   CCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTT                                                                                                    <  SRR19973063.817661/150‑1 (MQ=60)
                                                    CTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTT                                                                                                   <  SRR19973063.887339/150‑1 (MQ=60)
                                                    CTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTT                                                                                                   <  SRR19973063.5011589/150‑1 (MQ=60)
                                                    CTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTT                                                                                                   <  SRR19973063.3432544/150‑1 (MQ=60)
                                                    CTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTT                                                                                                   >  SRR19973063.4009487/1‑150 (MQ=60)
                                                    CTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTT                                                                                                   >  SRR19973063.4009781/1‑150 (MQ=60)
                                                      GCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGT                                                                                                 <  SRR19973063.2451863/150‑1 (MQ=60)
                                                           CGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTT                                                                                            <  SRR19973063.3305847/150‑1 (MQ=60)
                                                             TGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCAGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAA                                                                                          >  SRR19973063.3076368/1‑150 (MQ=60)
                                                              GCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAA                                                                                         <  SRR19973063.1484811/150‑1 (MQ=60)
                                                               CCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAAT                                                                                        <  SRR19973063.4220182/150‑1 (MQ=60)
                                                               CCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAAT                                                                                        <  SRR19973063.1746139/150‑1 (MQ=60)
                                                                CCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATA                                                                                       <  SRR19973063.643880/150‑1 (MQ=60)
                                                                CCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATA                                                                                       <  SRR19973063.643801/150‑1 (MQ=60)
                                                                  TGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAA                                                                                     >  SRR19973063.2201060/1‑150 (MQ=60)
                                                                  TGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAA                                                                                     >  SRR19973063.3900608/1‑150 (MQ=60)
                                                                   GGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAAT                                                                                    >  SRR19973063.2094984/1‑150 (MQ=60)
                                                                     TTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTC                                                                                  <  SRR19973063.3037338/150‑1 (MQ=60)
                                                                      TAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCA                                                                                 >  SRR19973063.1202022/1‑150 (MQ=60)
                                                                       AGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCAC                                                                                >  SRR19973063.1151049/1‑150 (MQ=60)
                                                                        GAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACC                                                                               <  SRR19973063.5018803/150‑1 (MQ=60)
                                                                        GAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACC                                                                               <  SRR19973063.2749345/150‑1 (MQ=60)
                                                                         AAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCA                                                                              >  SRR19973063.3735275/1‑150 (MQ=60)
                                                                         AAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCA                                                                              >  SRR19973063.3735116/1‑150 (MQ=60)
                                                                          AAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCAT                                                                             >  SRR19973063.659460/1‑150 (MQ=60)
                                                                             CTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGAT                                                                          <  SRR19973063.975750/150‑1 (MQ=60)
                                                                                 GTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCA                                                                      <  SRR19973063.829882/150‑1 (MQ=60)
                                                                                   ACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAG                                                                    >  SRR19973063.2970954/1‑150 (MQ=60)
                                                                                         AACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCG                                                              <  SRR19973063.1044697/150‑1 (MQ=60)
                                                                                          ACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGC                                                             <  SRR19973063.1554528/150‑1 (MQ=60)
                                                                                            GCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCC                                                           >  SRR19973063.1665672/1‑150 (MQ=60)
                                                                                              TGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGA                                                         >  SRR19973063.1670557/1‑150 (MQ=60)
                                                                                                ATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACA                                                       <  SRR19973063.3727292/150‑1 (MQ=60)
                                                                                                ATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACA                                                       <  SRR19973063.3729670/150‑1 (MQ=60)
                                                                                                  GTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATG                                                     <  SRR19973063.1104275/150‑1 (MQ=60)
                                                                                                  GTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATG                                                     >  SRR19973063.4930381/1‑150 (MQ=60)
                                                                                                  GTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATG                                                     <  SRR19973063.1104188/150‑1 (MQ=60)
                                                                                                   TGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGC                                                    <  SRR19973063.3629627/150‑1 (MQ=60)
                                                                                                   TGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGC                                                    <  SRR19973063.3629628/150‑1 (MQ=60)
                                                                                                    GCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCG                                                   <  SRR19973063.1017495/150‑1 (MQ=60)
                                                                                                     CTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGC                                                  >  SRR19973063.1168580/1‑150 (MQ=60)
                                                                                                        ACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCC                                               <  SRR19973063.2581356/150‑1 (MQ=60)
                                                                                                         CTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATTCGCCCGACATGCGCGCCC                                              >  SRR19973063.150402/1‑150 (MQ=60)
                                                                                                          TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCA                                             <  SRR19973063.3237494/150‑1 (MQ=60)
                                                                                                              CGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGGCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCC                                         >  SRR19973063.3883970/1‑150 (MQ=60)
                                                                                                              CGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCC                                         >  SRR19973063.3246738/1‑150 (MQ=60)
                                                                                                              CGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCC                                         >  SRR19973063.3246579/1‑150 (MQ=60)
                                                                                                              CGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCC                                         <  SRR19973063.1680975/150‑1 (MQ=60)
                                                                                                              CGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCC                                         >  SRR19973063.596707/1‑150 (MQ=60)
                                                                                                              CGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCC                                         >  SRR19973063.596785/1‑150 (MQ=60)
                                                                                                                CAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGTAGAGTATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGG                                       <  SRR19973063.4789321/150‑1 (MQ=60)
                                                                                                                 AGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGT                                      >  SRR19973063.3091862/1‑150 (MQ=60)
                                                                                                                 AGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGT                                      >  SRR19973063.2491489/1‑150 (MQ=60)
                                                                                                                 AGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGT                                      >  SRR19973063.3817169/1‑150 (MQ=60)
                                                                                                                  GGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTA                                     >  SRR19973063.4704921/1‑150 (MQ=60)
                                                                                                                   GAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAA                                    <  SRR19973063.4353132/150‑1 (MQ=60)
                                                                                                                    AAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAAT                                   <  SRR19973063.4601788/150‑1 (MQ=60)
                                                                                                                      GGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGA                                 <  SRR19973063.2880621/150‑1 (MQ=60)
                                                                                                                      GGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGA                                 >  SRR19973063.2897617/1‑150 (MQ=60)
                                                                                                                       GGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGAT                                >  SRR19973063.2885575/1‑150 (MQ=60)
                                                                                                                       GGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGAT                                >  SRR19973063.2885616/1‑150 (MQ=60)
                                                                                                                       GGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGAT                                >  SRR19973063.3636591/1‑150 (MQ=60)
                                                                                                                         CCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTAACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGT                              >  SRR19973063.456133/1‑150 (MQ=60)
                                                                                                                          CTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTC                             >  SRR19973063.4039457/1‑150 (MQ=60)
                                                                                                                          CTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTC                             <  SRR19973063.3096869/150‑1 (MQ=60)
                                                                                                                              TGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTT                         <  SRR19973063.3254457/150‑1 (MQ=60)
                                                                                                                              TGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTT                         <  SRR19973063.3028246/150‑1 (MQ=60)
                                                                                                                              TGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTT                         <  SRR19973063.3028299/150‑1 (MQ=60)
                                                                                                                               GACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTG                        >  SRR19973063.3421068/1‑150 (MQ=60)
                                                                                                                                 CGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCC                      >  SRR19973063.4226561/1‑150 (MQ=60)
                                                                                                                                  GGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCA                     <  SRR19973063.2233129/150‑1 (MQ=60)
                                                                                                                                  GGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCA                     >  SRR19973063.4231144/1‑150 (MQ=60)
                                                                                                                                  GGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCA                     >  SRR19973063.3909239/1‑150 (MQ=60)
                                                                                                                                     TGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTA                  >  SRR19973063.874345/1‑150 (MQ=60)
                                                                                                                                     TGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTA                  >  SRR19973063.874224/1‑150 (MQ=60)
                                                                                                                                      GCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAG                 <  SRR19973063.2589302/150‑1 (MQ=60)
                                                                                                                                        TGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGAT               <  SRR19973063.4212344/150‑1 (MQ=60)
                                                                                                                                        TGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGAT               <  SRR19973063.4212333/150‑1 (MQ=60)
                                                                                                                                          TCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCG             <  SRR19973063.2796035/150‑1 (MQ=60)
                                                                                                                                            TTAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCC           >  SRR19973063.1405671/1‑150 (MQ=60)
                                                                                                                                            TGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCC           >  SRR19973063.4100117/1‑150 (MQ=60)
                                                                                                                                                 TGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCA      <  SRR19973063.3306295/150‑1 (MQ=60)
                                                                                                                                                 TGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCA      <  SRR19973063.3711233/150‑1 (MQ=60)
                                                                                                                                                 TGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCA      <  SRR19973063.3711543/150‑1 (MQ=60)
                                                                                                                                                  GACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAA     >  SRR19973063.109130/1‑150 (MQ=60)
                                                                                                                                                    CAGGGAAGATGATATCTGCCGCTTTACTTAACGGTGAGCCAATCTGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCAAGTAGATCGCCGCGCAATG   <  SRR19973063.4772678/150‑1 (MQ=60)
                                                                                                                                                     AGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGC  >  SRR19973063.1852385/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGC  >  CP009273/1661614‑1661912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑