Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,852,274 C→A 100% D273Y (GAT→TAT)  ydjJ ← putative Zn‑dependent NAD(P)‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,852,2740CA99.3% 792.0 / ‑4.2 238D273Y (GAT→TAT) ydjJputative Zn‑dependent NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (119/117);  total (120/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 238 total aligned reads displayed.

GATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATCGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAGTGT  >  CP009273/1852127‑1852420
                                                                                                                                                   |                                                                                                                                                  
gATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGc                                                                                                                                                  >  1:332187/1‑150 (MQ=255)
  tAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCg                                                                                                                                                <  2:1883201/150‑1 (MQ=255)
  tAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGAGTGATTTTGAGGAAATTGATTGCCGAATAGCCg                                                                                                                                                >  2:1155773/1‑150 (MQ=255)
   aaTCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCgg                                                                                                                                               >  2:2970594/1‑150 (MQ=255)
   aaTCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCgg                                                                                                                                               >  1:2983519/1‑150 (MQ=255)
    aTCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCggg                                                                                                                                              <  2:29227/150‑1 (MQ=255)
     tCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGt                                                                                                                                             >  2:2444581/1‑150 (MQ=255)
      cGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTa                                                                                                                                            <  2:2852773/150‑1 (MQ=255)
       gTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTAc                                                                                                                                           >  2:45093/1‑150 (MQ=255)
        tAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACa                                                                                                                                          <  1:2864609/150‑1 (MQ=255)
        tAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACa                                                                                                                                          >  1:1594733/1‑150 (MQ=255)
         aaaTATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAg                                                                                                                                         >  1:1805286/1‑150 (MQ=255)
          aaTATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGt                                                                                                                                        <  1:2666458/150‑1 (MQ=255)
          aaTATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGt                                                                                                                                        <  2:4230232/150‑1 (MQ=255)
          aaTATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGt                                                                                                                                        <  1:65217/150‑1 (MQ=255)
           atatGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTa                                                                                                                                       >  1:1197704/1‑150 (MQ=255)
            tatGAGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTAc                                                                                                                                      >  2:4610393/1‑150 (MQ=255)
              tGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACca                                                                                                                                    >  2:2649278/1‑150 (MQ=255)
              tGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACca                                                                                                                                    <  1:1310160/150‑1 (MQ=255)
                cGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACcaac                                                                                                                                  <  1:5021277/150‑1 (MQ=255)
                   cACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAAt                                                                                                                               <  1:4678285/150‑1 (MQ=255)
                      cATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCAt                                                                                                                            >  2:542349/1‑150 (MQ=255)
                         cGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAAt                                                                                                                         >  1:1935641/1‑150 (MQ=255)
                          gATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAAtt                                                                                                                        >  1:1643348/1‑150 (MQ=255)
                            tttCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAAtttt                                                                                                                      <  2:4334523/150‑1 (MQ=255)
                            tttCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAAtttt                                                                                                                      <  1:2770555/150‑1 (MQ=255)
                             ttCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTa                                                                                                                     >  2:2578128/1‑150 (MQ=255)
                             ttCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTa                                                                                                                     >  2:4694703/1‑150 (MQ=255)
                              tCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTAc                                                                                                                    <  2:4004775/150‑1 (MQ=255)
                                    cGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGACTAGCCGGGTACAGTACCAACAATCATAATTTTACcgccgc                                                                                                              >  1:4758564/1‑150 (MQ=255)
                                     gAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGACGGGTACAGTACCAACAATCAAAATTTTACCGAcgcg                                                                                                             >  2:4273386/1‑150 (MQ=255)
                                        tCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCAt                                                                                                          <  1:2403764/150‑1 (MQ=255)
                                             cTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCa                                                                                                     >  1:3375563/1‑150 (MQ=255)
                                              tGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAg                                                                                                    <  1:3761933/150‑1 (MQ=255)
                                              tGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAg                                                                                                    >  2:2355369/1‑150 (MQ=255)
                                              tGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAg                                                                                                    <  1:1541368/150‑1 (MQ=255)
                                                aagaaATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGAt                                                                                                  >  1:278188/1‑150 (MQ=255)
                                                 agaaATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATa                                                                                                 >  2:554215/1‑150 (MQ=255)
                                                 agaaATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATa                                                                                                 >  2:5031295/1‑150 (MQ=255)
                                                      tAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTg                                                                                            >  1:4074509/1‑150 (MQ=255)
                                                      tAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTg                                                                                            >  2:1085734/1‑150 (MQ=255)
                                                           tCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGt                                                                                       <  2:713525/150‑1 (MQ=255)
                                                            cAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGtt                                                                                      <  1:4920094/150‑1 (MQ=255)
                                                             aaTCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGttt                                                                                     >  2:3772889/1‑150 (MQ=255)
                                                              aTCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATCGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGAGCCTGTTTa                                                                                    >  2:4804315/1‑150 (MQ=255)
                                                              aTCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTa                                                                                    <  2:197654/150‑1 (MQ=255)
                                                              aTCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTa                                                                                    <  2:2130314/150‑1 (MQ=255)
                                                                       ggATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGAcc                                                                           >  1:1377286/1‑150 (MQ=255)
                                                                       ggATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGAcc                                                                           <  2:1614614/150‑1 (MQ=255)
                                                                         aTAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGc                                                                         >  1:2437626/1‑150 (MQ=255)
                                                                           aaCGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAg                                                                       <  2:3084911/150‑1 (MQ=255)
                                                                             cGATTGGTATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGaa                                                                     >  2:1423791/1‑150 (MQ=255)
                                                                                   gCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCt                                                               >  2:1554540/1‑150 (MQ=255)
                                                                                    cATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTg                                                              <  1:2621042/150‑1 (MQ=255)
                                                                                    cATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTg                                                              <  1:607372/150‑1 (MQ=255)
                                                                                     aTAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGt                                                             <  1:3836671/150‑1 (MQ=255)
                                                                                      tAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGtt                                                            >  1:5154073/1‑150 (MQ=255)
                                                                                       aGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGttt                                                           >  2:4877909/1‑150 (MQ=255)
                                                                                        gCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTc                                                          >  1:2033530/1‑150 (MQ=255)
                                                                                         cGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCg                                                         >  1:4406253/1‑150 (MQ=255)
                                                                                         cGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCg                                                         <  1:3806278/150‑1 (MQ=255)
                                                                                         cGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCg                                                         >  1:3607199/1‑150 (MQ=255)
                                                                                          ggAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCAATACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGa                                                        >  1:4803152/1‑150 (MQ=255)
                                                                                                ccGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACaa                                                  >  2:3268290/1‑150 (MQ=255)
                                                                                                     tGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCt                                             >  1:2006541/1‑150 (MQ=255)
                                                                                                           gTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGccc                                       >  1:1560990/1‑150 (MQ=255)
                                                                                                           gTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGccc                                       >  1:429350/1‑150 (MQ=255)
                                                                                                            tGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCa                                      <  2:95440/150‑1 (MQ=255)
                                                                                                            tGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCa                                      >  2:2749471/1‑150 (MQ=255)
                                                                                                            tGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCa                                      >  1:5056822/1‑150 (MQ=255)
                                                                                                             taCTTCGATATTGATTTTGAGGAAATTGATTGCCGAATATCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTAAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCAt                                     >  2:121712/2‑150 (MQ=255)
                                                                                                             gACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCAt                                     <  1:4940761/150‑1 (MQ=255)
                                                                                                              aCTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATg                                    <  1:4340077/150‑1 (MQ=255)
                                                                                                              aCTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATg                                    <  2:3606457/150‑1 (MQ=255)
                                                                                                                 tCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCt                                 <  2:1530169/150‑1 (MQ=255)
                                                                                                                   gcgATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTc                               <  1:2226202/150‑1 (MQ=255)
                                                                                                                   gcgATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTc                               <  2:3696267/150‑1 (MQ=255)
                                                                                                                    cgATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCg                              <  1:2799115/150‑1 (MQ=255)
                                                                                                                     gattgattTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCgg                             <  2:918975/150‑1 (MQ=255)
                                                                                                                        tgattTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTaa                          >  1:3485942/1‑150 (MQ=255)
                                                                                                                          attTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAAt                        <  1:1155773/150‑1 (MQ=255)
                                                                                                                            tttGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTg                      <  2:4085350/150‑1 (MQ=255)
                                                                                                                              tGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCt                    <  2:1860863/150‑1 (MQ=255)
                                                                                                                              tGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCt                    >  2:2070121/1‑150 (MQ=255)
                                                                                                                              tGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCt                    >  2:770448/1‑150 (MQ=255)
                                                                                                                               gatgAAATTGATTGACGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTg                   <  2:4402742/147‑1 (MQ=255)
                                                                                                                               gAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTg                   <  2:2490022/150‑1 (MQ=255)
                                                                                                                                aGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGa                  >  2:342620/1‑150 (MQ=255)
                                                                                                                                aGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGa                  >  1:149090/1‑150 (MQ=255)
                                                                                                                                   aaaTTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAg               <  2:3945089/150‑1 (MQ=255)
                                                                                                                                   aaaTTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAg               >  2:3081921/1‑150 (MQ=255)
                                                                                                                                      ttgattgCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGt            <  1:1044277/150‑1 (MQ=255)
                                                                                                                                         attgCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCa         >  2:2346166/1‑150 (MQ=255)
                                                                                                                                           tgCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAAt       >  1:318824/1‑150 (MQ=255)
                                                                                                                                           tgCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAAt       <  2:722829/150‑1 (MQ=255)
                                                                                                                                             ccGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAg     >  1:4006020/1‑150 (MQ=255)
                                                                                                                                              cGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAgt    >  1:4514438/1‑150 (MQ=255)
                                                                                                                                               gAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAgtg   >  1:2065930/1‑150 (MQ=255)
                                                                                                                                               gAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAgtg   >  2:4116214/1‑150 (MQ=255)
                                                                                                                                                aaTAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAgtgt  >  1:2515691/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATCGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAGTGT  >  CP009273/1852127‑1852420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATCGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAGTGTCT  >  CP009273/1852125‑1852422
                                                                                                                                                     |                                                                                                                                                    
CCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATA                                                                                                                                                      >  SRR19973063.2186603/1‑150 (MQ=60)
CCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATA                                                                                                                                                      <  SRR19973063.3818500/150‑1 (MQ=60)
CCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATA                                                                                                                                                      <  SRR19973063.4616160/150‑1 (MQ=60)
CCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATA                                                                                                                                                      <  SRR19973063.1411650/150‑1 (MQ=60)
CCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATA                                                                                                                                                      >  SRR19973063.1496767/1‑150 (MQ=60)
CCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATA                                                                                                                                                      >  SRR19973063.1496683/1‑150 (MQ=60)
  GATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGC                                                                                                                                                    >  SRR19973063.333146/1‑150 (MQ=60)
  GATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGC                                                                                                                                                    >  SRR19973063.333147/1‑150 (MQ=60)
     AATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGG                                                                                                                                                 <  SRR19973063.2430220/150‑1 (MQ=60)
     AATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGG                                                                                                                                                 >  SRR19973063.2993047/1‑150 (MQ=60)
     AATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGG                                                                                                                                                 >  SRR19973063.1580460/1‑150 (MQ=60)
     AATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGG                                                                                                                                                 <  SRR19973063.4997652/150‑1 (MQ=60)
      ATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGG                                                                                                                                                <  SRR19973063.403638/150‑1 (MQ=60)
      ATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGG                                                                                                                                                <  SRR19973063.4723281/150‑1 (MQ=60)
       TCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGT                                                                                                                                               <  SRR19973063.1337291/150‑1 (MQ=60)
          TAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACA                                                                                                                                            <  SRR19973063.2873788/150‑1 (MQ=60)
          TAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACA                                                                                                                                            >  SRR19973063.1599626/1‑150 (MQ=60)
          TAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACA                                                                                                                                            >  SRR19973063.527235/1‑150 (MQ=60)
           AAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAG                                                                                                                                           >  SRR19973063.1810889/1‑150 (MQ=60)
            AATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGT                                                                                                                                          <  SRR19973063.65438/150‑1 (MQ=60)
            AATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGT                                                                                                                                          <  SRR19973063.2675016/150‑1 (MQ=60)
             ATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTA                                                                                                                                         >  SRR19973063.1201298/1‑150 (MQ=60)
                TGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCA                                                                                                                                      <  SRR19973063.1314064/150‑1 (MQ=60)
                  CGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAAC                                                                                                                                    <  SRR19973063.5037479/150‑1 (MQ=60)
                  CGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAAGACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAAC                                                                                                                                    <  SRR19973063.5037459/150‑1 (MQ=60)
                     CACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAAT                                                                                                                                 <  SRR19973063.4693204/150‑1 (MQ=60)
                      ACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATC                                                                                                                                <  SRR19973063.3238997/150‑1 (MQ=60)
                          TCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAA                                                                                                                            <  SRR19973063.76199/150‑1 (MQ=60)
                           CGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAAT                                                                                                                           >  SRR19973063.1941692/1‑150 (MQ=60)
                            GATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATT                                                                                                                          >  SRR19973063.1648372/1‑150 (MQ=60)
                              TTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTT                                                                                                                        <  SRR19973063.2779488/150‑1 (MQ=60)
                                    ATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCC                                                                                                                  <  SRR19973063.1173790/150‑1 (MQ=60)
                                      CGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGACTAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGC                                                                                                                >  SRR19973063.4773738/1‑150 (MQ=60)
                                        AATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGC                                                                                                              >  SRR19973063.3957708/1‑150 (MQ=60)
                                          TCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCAT                                                                                                            <  SRR19973063.95636/150‑1 (MQ=60)
                                          TCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCAT                                                                                                            <  SRR19973063.2411453/150‑1 (MQ=60)
                                               CTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCA                                                                                                       <  SRR19973063.1053707/150‑1 (MQ=60)
                                               CTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCA                                                                                                       >  SRR19973063.3386316/1‑150 (MQ=60)
                                                TGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAG                                                                                                      <  SRR19973063.1546347/150‑1 (MQ=60)
                                                TGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAG                                                                                                      <  SRR19973063.1545670/150‑1 (MQ=60)
                                                TGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAG                                                                                                      <  SRR19973063.3773955/150‑1 (MQ=60)
                                                TGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAG                                                                                                      <  SRR19973063.3773951/150‑1 (MQ=60)
                                                TGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAG                                                                                                      <  SRR19973063.1546092/150‑1 (MQ=60)
                                                  AAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGAT                                                                                                    >  SRR19973063.279014/1‑150 (MQ=60)
                                                  AAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGAT                                                                                                    >  SRR19973063.279003/1‑150 (MQ=60)
                                                  AAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGAT                                                                                                    <  SRR19973063.3480868/150‑1 (MQ=60)
                                                  AAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGAT                                                                                                    <  SRR19973063.3480834/150‑1 (MQ=60)
                                                        TAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTG                                                                                              >  SRR19973063.4087563/1‑150 (MQ=60)
                                                         AGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGC                                                                                             >  SRR19973063.1227298/1‑150 (MQ=60)
                                                         AGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGC                                                                                             >  SRR19973063.2473566/1‑150 (MQ=60)
                                                              CAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTT                                                                                        <  SRR19973063.4935983/150‑1 (MQ=60)
                                                                ATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTA                                                                                      >  SRR19973063.1749523/1‑150 (MQ=60)
                                                                   GTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACG                                                                                   >  SRR19973063.2761510/1‑150 (MQ=60)
                                                                        CGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGAC                                                                              <  SRR19973063.280263/150‑1 (MQ=60)
                                                                         GGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACC                                                                             >  SRR19973063.1381438/1‑150 (MQ=60)
                                                                         GGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACC                                                                             >  SRR19973063.1518412/1‑150 (MQ=60)
                                                                           ATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGC                                                                           >  SRR19973063.2445417/1‑150 (MQ=60)
                                                                             AACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAG                                                                         >  SRR19973063.2780870/1‑150 (MQ=60)
                                                                               CGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAA                                                                       <  SRR19973063.2122362/150‑1 (MQ=60)
                                                                               CGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAA                                                                       >  SRR19973063.4536194/1‑150 (MQ=60)
                                                                                GATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAAC                                                                      >  SRR19973063.3293922/1‑150 (MQ=60)
                                                                                 ATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACC                                                                     >  SRR19973063.1602421/1‑150 (MQ=60)
                                                                                  TTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCC                                                                    <  SRR19973063.6276/150‑1 (MQ=60)
                                                                                   TGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCG                                                                   <  SRR19973063.4936807/150‑1 (MQ=60)
                                                                                     GCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCT                                                                 <  SRR19973063.4329931/150‑1 (MQ=60)
                                                                                     GCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCT                                                                 <  SRR19973063.4330237/150‑1 (MQ=60)
                                                                                      CATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTG                                                                >  SRR19973063.1277435/1‑150 (MQ=60)
                                                                                      CATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTG                                                                >  SRR19973063.4783699/1‑150 (MQ=60)
                                                                                      CATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTG                                                                <  SRR19973063.609152/150‑1 (MQ=60)
                                                                                      CATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTG                                                                <  SRR19973063.2629389/150‑1 (MQ=60)
                                                                                      CATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGACGGGTACAGTACCAACAATCATAATAATAACGCCGAGCATTACAAGATAAGGTGCCTTTTTAACGGTGACCGCAGAACCCGATG                                                                >  SRR19973063.5057138/1‑150 (MQ=60)
                                                                                       ATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGT                                                               <  SRR19973063.3848874/150‑1 (MQ=60)
                                                                                        TAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTT                                                              <  SRR19973063.1403236/150‑1 (MQ=60)
                                                                                        TAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTT                                                              >  SRR19973063.5170655/1‑150 (MQ=60)
                                                                                         AGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTT                                                             <  SRR19973063.2639432/150‑1 (MQ=60)
                                                                                          GCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTC                                                            >  SRR19973063.2039887/1‑150 (MQ=60)
                                                                                           CGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCG                                                           >  SRR19973063.3618779/1‑150 (MQ=60)
                                                                                           CGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCG                                                           >  SRR19973063.4420383/1‑150 (MQ=60)
                                                                                           CGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCG                                                           <  SRR19973063.3818398/150‑1 (MQ=60)
                                                                                            GGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCAATACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGA                                                          >  SRR19973063.4818508/1‑150 (MQ=60)
                                                                                                  CCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAA                                                    >  SRR19973063.2979235/1‑150 (MQ=60)
                                                                                                      CTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATC                                                <  SRR19973063.1997653/150‑1 (MQ=60)
                                                                                                       TGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCT                                               >  SRR19973063.4472807/1‑150 (MQ=60)
                                                                                                       TGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCT                                               >  SRR19973063.2013045/1‑150 (MQ=60)
                                                                                                       TGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCT                                               >  SRR19973063.2012809/1‑150 (MQ=60)
                                                                                                          ATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCG                                            >  SRR19973063.490212/1‑150 (MQ=60)
                                                                                                          ATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCG                                            >  SRR19973063.490208/1‑150 (MQ=60)
                                                                                                             GTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCC                                         >  SRR19973063.430545/1‑150 (MQ=60)
                                                                                                             GTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCC                                         >  SRR19973063.3735792/1‑150 (MQ=60)
                                                                                                             GTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCC                                         >  SRR19973063.1565779/1‑150 (MQ=60)
                                                                                                              TGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCA                                        >  SRR19973063.5073102/1‑150 (MQ=60)
                                                                                                               GACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCAT                                       <  SRR19973063.4956735/150‑1 (MQ=60)
                                                                                                               GACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCAT                                       <  SRR19973063.2624607/150‑1 (MQ=60)
                                                                                                                ACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATG                                      <  SRR19973063.4353918/150‑1 (MQ=60)
                                                                                                                ACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATG                                      <  SRR19973063.3547235/150‑1 (MQ=60)
                                                                                                                     GCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTC                                 <  SRR19973063.2233253/150‑1 (MQ=60)
                                                                                                                     GCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTC                                 <  SRR19973063.2233303/150‑1 (MQ=60)
                                                                                                                      CGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCG                                >  SRR19973063.521839/1‑150 (MQ=60)
                                                                                                                      CGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCG                                <  SRR19973063.2808111/150‑1 (MQ=60)
                                                                                                                        ATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGT                              >  SRR19973063.4391380/1‑150 (MQ=60)
                                                                                                                        ATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGT                              >  SRR19973063.4537913/1‑150 (MQ=60)
                                                                                                                         TTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTA                             <  SRR19973063.2559152/150‑1 (MQ=60)
                                                                                                                          TGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAA                            >  SRR19973063.3497079/1‑150 (MQ=60)
                                                                                                                            ATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAAT                          <  SRR19973063.5166938/150‑1 (MQ=60)
                                                                                                                            ATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAAT                          <  SRR19973063.1159260/150‑1 (MQ=60)
                                                                                                                             TTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATT                         <  SRR19973063.2362908/150‑1 (MQ=60)
                                                                                                                                 GAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTG                     <  SRR19973063.3066227/150‑1 (MQ=60)
                                                                                                                                  AGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGA                    <  SRR19973063.2860790/150‑1 (MQ=60)
                                                                                                                                  AGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGA                    >  SRR19973063.149551/1‑150 (MQ=60)
                                                                                                                                   GGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGAC                   >  SRR19973063.3840062/1‑150 (MQ=60)
                                                                                                                                    GAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACA                  <  SRR19973063.2817074/150‑1 (MQ=60)
                                                                                                                                    GAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACA                  <  SRR19973063.1820407/150‑1 (MQ=60)
                                                                                                                                    GAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACA                  <  SRR19973063.1820369/150‑1 (MQ=60)
                                                                                                                                    GAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACA                  >  SRR19973063.4085048/1‑150 (MQ=60)
                                                                                                                                     AAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAG                 >  SRR19973063.3804817/1‑150 (MQ=60)
                                                                                                                                        TTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGT              <  SRR19973063.1047440/150‑1 (MQ=60)
                                                                                                                                        TTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGT              >  SRR19973063.4157888/1‑150 (MQ=60)
                                                                                                                                             TGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAAT         >  SRR19973063.319756/1‑150 (MQ=60)
                                                                                                                                               CCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAG       >  SRR19973063.4018868/1‑150 (MQ=60)
                                                                                                                                               CCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAG       >  SRR19973063.1587995/1‑150 (MQ=60)
                                                                                                                                                CGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAGT      >  SRR19973063.4528930/1‑150 (MQ=60)
                                                                                                                                                 GAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAGTG     >  SRR19973063.2072313/1‑150 (MQ=60)
                                                                                                                                                 GAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAGTG     >  SRR19973063.2072326/1‑150 (MQ=60)
                                                                                                                                                 GAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAGTG     >  SRR19973063.2072388/1‑150 (MQ=60)
                                                                                                                                                  AATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAGTGT    >  SRR19973063.2523712/1‑150 (MQ=60)
                                                                                                                                                    TAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAGTGTCT  <  SRR19973063.543954/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATCGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAGTGTCT  >  CP009273/1852125‑1852422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑