Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,859,132 A→C 100% A254A (GCT→GCG)  yeaE ← aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,859,1320AC98.3% 921.6 / ‑0.5 240A254A (GCT→GCG) yeaEaldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base C (115/120);  total (117/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 241 total aligned reads displayed.

CGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACA  >  CP009273/1858986‑1859277
                                                                                                                                                  |                                                                                                                                                 
cGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGcc                                                                                                                                                <  1:2992193/150‑1 (MQ=255)
   ttttttACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACTAAACATCACACCATATCCAGCGCAGTTTTTACTTTTGGTGCCTGTTATGCATTATCCATCATAGCTTATTCCGCTGAAGAAAGTTAGACCTCAAGCACCGCCGCa                                                                                                                                             <  2:2421918/147‑1 (MQ=255)
       ttACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCAtttt                                                                                                                                         <  2:4047803/150‑1 (MQ=255)
         aCGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTttgt                                                                                                                                       >  2:5124104/1‑150 (MQ=255)
           gTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTttgttg                                                                                                                                     <  1:299128/150‑1 (MQ=255)
           gTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTttgttg                                                                                                                                     <  1:1949015/150‑1 (MQ=255)
            ttAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTgg                                                                                                                                    <  2:4895696/150‑1 (MQ=255)
             tattgttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGa                                                                                                                                   <  1:3572817/147‑1 (MQ=255)
             tattgttgtGGGCCGGGTGCAAGGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGa                                                                                                                                   <  1:992693/147‑1 (MQ=255)
             tAATGTTGTGTGCCGGGTGCAAAGCATCCGGCACACAAAATAACACCATATCCCGCGCAGTTTTTCCTTTTGGTGCCGGAAATGCCTTATCCAGCATAGCTAATTACGCTGAATCAAGTTCGAACTCAATCACCCCCGCATTTTGTTGGa                                                                                                                                   >  2:1125544/1‑150 (MQ=255)
              attgttgtGTGCCGGGTGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGAc                                                                                                                                  <  1:4760628/148‑1 (MQ=255)
                   tgtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATggg                                                                                                                             <  2:1871527/150‑1 (MQ=255)
                   tgtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATggg                                                                                                                             <  1:4771851/150‑1 (MQ=255)
                     tttgCCGGGTGCAATGCATCCGTCACACAACATCACACCATATCCTGCTCATTTTTTACTTTTTTTGCCGGATATGCCTTATCCAGCTTAGCTATTTCCGCTGAAGAAAGTTCGACCTAAAGCACCGCCGCATTTTGTTGGACATGGGCa                                                                                                                           <  2:1559656/148‑1 (MQ=255)
                     tgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCa                                                                                                                           >  2:4291389/1‑150 (MQ=255)
                      gtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCaa                                                                                                                          >  1:549371/1‑150 (MQ=255)
                         ccGGGTGCAATGCATCCGCCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCg                                                                                                                       <  1:4370724/150‑1 (MQ=255)
                           gggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTg                                                                                                                     >  1:258161/1‑150 (MQ=255)
                            ggTGCAATGCATCCGGCACACAACATCACACCATCTCCAGCGCAGTTGTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTgg                                                                                                                    <  2:4667973/150‑1 (MQ=255)
                            ggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTgg                                                                                                                    <  1:2042641/150‑1 (MQ=255)
                              tGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGcc                                                                                                                  <  1:4214361/150‑1 (MQ=255)
                                   tGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCttt                                                                                                             <  2:1565630/150‑1 (MQ=255)
                                    gCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGTCAATCGTGGCCGCtttt                                                                                                            >  2:22194/1‑150 (MQ=255)
                                      aTCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTgg                                                                                                          >  1:4952913/1‑150 (MQ=255)
                                         cGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAAt                                                                                                       <  2:2865783/150‑1 (MQ=255)
                                         cGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAAt                                                                                                       >  2:5009271/1‑150 (MQ=255)
                                           gCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCg                                                                                                     >  2:3991807/1‑150 (MQ=255)
                                            cacacaACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGc                                                                                                    >  2:311529/1‑150 (MQ=255)
                                             acacaACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGcc                                                                                                   >  1:1457123/1‑150 (MQ=255)
                                              cacaACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCa                                                                                                  >  1:2937730/1‑150 (MQ=255)
                                               acaacaTCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCAt                                                                                                 <  1:4572197/150‑1 (MQ=255)
                                                   caTCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCGTTTGGAATCGCCATcaca                                                                                             >  1:497879/1‑150 (MQ=255)
                                                     tCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACAcc                                                                                           >  2:2034129/1‑150 (MQ=255)
                                                     tCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACAcc                                                                                           <  1:4151219/150‑1 (MQ=255)
                                                       acacCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCt                                                                                         <  1:3344988/150‑1 (MQ=255)
                                                       acacCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCt                                                                                         <  2:552020/150‑1 (MQ=255)
                                                       acacCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCt                                                                                         <  2:2395448/150‑1 (MQ=255)
                                                        cacCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCtg                                                                                        <  1:4350854/150‑1 (MQ=255)
                                                         acCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCGTTTGGAATCGCCATCACACCCtga                                                                                       >  2:4465697/1‑150 (MQ=255)
                                                          ccATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCtgat                                                                                      <  1:2489047/150‑1 (MQ=255)
                                                          ccATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCtgat                                                                                      <  1:136443/150‑1 (MQ=255)
                                                                 cAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGAt                                                                               <  2:1120553/150‑1 (MQ=255)
                                                                  aGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATc                                                                              >  2:337309/1‑150 (MQ=255)
                                                                      cAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCAccc                                                                          >  2:2697991/1‑150 (MQ=255)
                                                                       tgTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCa                                                                         >  2:134719/2‑150 (MQ=255)
                                                                        gTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCCGATGACTGATCACCCAc                                                                        >  1:4925294/1‑150 (MQ=255)
                                                                           tttCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGcc                                                                     <  2:2352398/150‑1 (MQ=255)
                                                                             tCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaa                                                                   <  2:2243371/150‑1 (MQ=255)
                                                                             tCCTTTTGGTGCCGGAGATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaa                                                                   <  1:4674288/150‑1 (MQ=255)
                                                                                ttttGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaacaa                                                                >  2:4215550/1‑150 (MQ=255)
                                                                                  ttGGTGCCGGATATGCCTTATCCATCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATa                                                              <  1:4562316/150‑1 (MQ=255)
                                                                                  ttGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCTCCGCATTTTGTTGGACATGGGCAATCGGGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATa                                                              <  1:2354424/150‑1 (MQ=255)
                                                                                    ggTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACt                                                            >  1:1076115/1‑150 (MQ=255)
                                                                                    ggTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACt                                                            >  1:3817927/1‑150 (MQ=255)
                                                                                        ccGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAACGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTtgtg                                                        >  1:738094/1‑150 (MQ=255)
                                                                                        ccGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTtgtg                                                        <  2:4952986/150‑1 (MQ=255)
                                                                                         cGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGc                                                       >  2:1223618/1‑150 (MQ=255)
                                                                                           gATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCcg                                                     <  2:1697552/150‑1 (MQ=255)
                                                                                             tatGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTAAATCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTAGGAATAGCCATCACAACCTGATTACTGATCACCCACGACAACAATACTtgttcagct                                                   >  1:1594361/1‑144 (MQ=255)
                                                                                              atGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTGGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCgcgc                                                  >  2:2367754/1‑150 (MQ=255)
                                                                                                 ccTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGa                                               <  1:4845770/150‑1 (MQ=255)
                                                                                                  cTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGat                                              <  1:1970677/150‑1 (MQ=255)
                                                                                                        cAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCACAATCACCGACGCATTTAGTTTGACATGGGCAATCTTGGCCGCTTTTGGAATCTCCATCACACCCTGATGACTGAACAACCACTCCAACAATACTTTTGCCTCGCTGATATtgtg                                        >  2:3097037/1‑150 (MQ=255)
                                                                                                         aGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGa                                       <  1:2178205/150‑1 (MQ=255)
                                                                                                         aGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGa                                       <  1:1769192/150‑1 (MQ=255)
                                                                                                            aTAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCa                                    >  2:896780/1‑150 (MQ=255)
                                                                                                             tAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCAt                                   >  2:2919791/1‑150 (MQ=255)
                                                                                                             tAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCAt                                   >  2:2994289/1‑150 (MQ=255)
                                                                                                               gCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCAtgt                                 >  1:723162/1‑150 (MQ=255)
                                                                                                                 tAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGc                               >  1:4077034/1‑150 (MQ=255)
                                                                                                                   aTTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCaa                             <  2:470974/150‑1 (MQ=255)
                                                                                                                   aTTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCaa                             >  2:3189870/1‑150 (MQ=255)
                                                                                                                     tCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt                           >  1:1863172/1‑150 (MQ=255)
                                                                                                                     tCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt                           <  1:2899732/150‑1 (MQ=255)
                                                                                                                     tCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt                           <  1:881067/150‑1 (MQ=255)
                                                                                                                      ccGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAttt                          <  1:1818869/150‑1 (MQ=255)
                                                                                                                       cGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTc                         <  1:3198200/150‑1 (MQ=255)
                                                                                                                         cTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGt                       <  2:4538065/150‑1 (MQ=255)
                                                                                                                         cTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAGTTCGt                       >  1:3014361/1‑150 (MQ=255)
                                                                                                                          tGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGtt                      >  1:1751226/1‑150 (MQ=255)
                                                                                                                          tGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGtt                      <  2:64753/150‑1 (MQ=255)
                                                                                                                           gaagaaAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTg                     <  2:2082784/150‑1 (MQ=255)
                                                                                                                           gaagaaAGTTCGACCTCAAGCACCGCCGCATTTTGGTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTg                     <  2:1268834/150‑1 (MQ=255)
                                                                                                                             agaaAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATAACACCCAGAAGACAGAACACCCACGCCAACAAAACGTTTGCCGCGCTGATATTGTGAGCAAGAGCAATATCGTTGAc                   >  1:2317163/1‑150 (MQ=255)
                                                                                                                               aaaGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACtc                 >  2:317380/1‑149 (MQ=255)
                                                                                                                                 aGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTAcc               >  2:3507676/1‑150 (MQ=255)
                                                                                                                                  gTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACcg              >  1:1691164/1‑150 (MQ=255)
                                                                                                                                  gTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACcg              <  1:2408760/150‑1 (MQ=255)
                                                                                                                                    tCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgcg            <  1:1125530/150‑1 (MQ=255)
                                                                                                                                      gACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCGTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGtt          >  1:4039894/1‑150 (MQ=255)
                                                                                                                                       aCCTCAAGCACAGCCGCATTTTGTTGGACATGGGCACTCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTCCCGCGttt         >  2:3236464/1‑150 (MQ=255)
                                                                                                                                        ccTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTCCCGCGtttt        <  2:377628/150‑1 (MQ=255)
                                                                                                                                        ccTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGtttt        <  2:1777424/150‑1 (MQ=255)
                                                                                                                                          tCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGtttttt      <  1:1248210/150‑1 (MQ=255)
                                                                                                                                          tCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGtttttt      >  2:2489174/1‑150 (MQ=255)
                                                                                                                                            aaGCACCGCCTCATATTGTTGGACATGTGCAATCGTGGCCGATATTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCAATTGCAATTTCGTTGACTACCGCGTTTTTTaa    >  2:4577958/1‑150 (MQ=255)
                                                                                                                                            aaGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTaa    >  2:5022166/1‑150 (MQ=255)
                                                                                                                                            aaGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTaa    >  2:1282936/1‑150 (MQ=255)
                                                                                                                                             aGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAAc   >  2:2832051/1‑150 (MQ=255)
                                                                                                                                             aGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATAGTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAAc   >  2:3870711/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACA  >  CP009273/1858986‑1859277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTC  >  CP009273/1858986‑1859280
                                                                                                                                                  |                                                                                                                                                    
CGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCC                                                                                                                                                   <  SRR19973063.3001677/150‑1 (MQ=60)
CGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCC                                                                                                                                                   <  SRR19973063.3001741/150‑1 (MQ=60)
CGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCC                                                                                                                                                   <  SRR19973063.4743732/150‑1 (MQ=60)
   TAATTTACGTTAATGTTGTGTGGCGGGTGCAATTCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTGCTGTTGGTGCCGGCTATGCCTTATCCAGCATAGCTAATTCCGCTGGAGAAAGTTAGACCTCAAGCACCGCCGCC                                                                                                                                                >  SRR19973063.4906341/1‑150 (MQ=60)
   TAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCA                                                                                                                                                <  SRR19973063.2009970/150‑1 (MQ=60)
      TTTACGTTAATGTTGTGTGCCGGGTGCAATGAATCACGCACACAACAACACACCATATCAACCGCAGTTTTTCCTTTTTGTGCCCGATATTACGTATCCACCATAGCTAATTACGCTGTAAAAAGTTCGACCTCAAGCACCGCCGCCTTT                                                                                                                                             >  SRR19973063.1762558/1‑150 (MQ=60)
       TTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTT                                                                                                                                            <  SRR19973063.5046765/150‑1 (MQ=60)
        TACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACCACATCACACCATATACAGCGCAGTTTTTCCTGTTGGTGCCGGATATGCCTTATACAGCATATCTAATTCCGCTGAAGAAAGTTAGAACTCAAGCACCGCCGCATTTTT                                                                                                                                           >  SRR19973063.234256/1‑150 (MQ=60)
           GTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTG                                                                                                                                        <  SRR19973063.300011/150‑1 (MQ=60)
           GTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTG                                                                                                                                        <  SRR19973063.1955117/150‑1 (MQ=60)
             TATTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGA                                                                                                                                      <  SRR19973063.3584273/150‑1 (MQ=60)
             TATTGTTGTGGGCCGGGTGCAAGGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGA                                                                                                                                      <  SRR19973063.995690/150‑1 (MQ=60)
              ATTGTTGTGTGCCGGGTGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGAC                                                                                                                                     <  SRR19973063.4775808/150‑1 (MQ=60)
              AATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGAC                                                                                                                                     <  SRR19973063.4775866/150‑1 (MQ=60)
               ATGTTGTGTGCCGGGTGAAATGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACA                                                                                                                                    <  SRR19973063.915627/150‑1 (MQ=60)
                   TGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGG                                                                                                                                <  SRR19973063.4787087/150‑1 (MQ=60)
                      GTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAA                                                                                                                             <  SRR19973063.791545/150‑1 (MQ=60)
                      GTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAA                                                                                                                             >  SRR19973063.550995/1‑150 (MQ=60)
                        GCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATC                                                                                                                           <  SRR19973063.421554/150‑1 (MQ=60)
                         CCGGGTGCAATGCATCCGCCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCG                                                                                                                          <  SRR19973063.4384707/150‑1 (MQ=60)
                           GGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTG                                                                                                                        >  SRR19973063.258934/1‑150 (MQ=60)
                            GGTGCAATGCATCCGGCACACAACATCACACCATCTCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGG                                                                                                                       <  SRR19973063.4341759/150‑1 (MQ=60)
                            GGTGCAATGCATCCGGCACACAACATCACACCATATCCCGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGATAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGACGCATTTTGTTGGACATGGGCAATCGTGG                                                                                                                       >  SRR19973063.4581156/1‑150 (MQ=60)
                            GGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGG                                                                                                                       <  SRR19973063.2049023/150‑1 (MQ=60)
                              TGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCC                                                                                                                     <  SRR19973063.4227804/150‑1 (MQ=60)
                               GCAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCG                                                                                                                    <  SRR19973063.4371019/150‑1 (MQ=60)
                                 AATGCATCCGGCACACAACATCACACAATATCAAGCGCATTTTTTCCTTTTGGTGCCGGATATGCCTTATACAGCATAGCTAAAACAGATGAAGAAAGTTCGAACTAAAGAACCGCCGCATTTAGTAGGAAATGGGCAATCGAGTCCGCT                                                                                                                  >  SRR19973063.2276759/1‑150 (MQ=60)
                                  ATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTT                                                                                                                 <  SRR19973063.4560459/150‑1 (MQ=60)
                                      ATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGG                                                                                                             <  SRR19973063.574866/150‑1 (MQ=60)
                                      ATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGG                                                                                                             >  SRR19973063.4968928/1‑150 (MQ=60)
                                      ATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGG                                                                                                             >  SRR19973063.4969001/1‑150 (MQ=60)
                                            CACACAACATCACACCATATCCAGCGCATTTTTACATTATGGTGCCGGATATGCCTTATCCAGCATAGCTAAAACAGCTGAAGAAAGTTCGACCTCAAGCACACCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTATTGGAATAGC                                                                                                       >  SRR19973063.1563344/1‑150 (MQ=60)
                                             ACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCC                                                                                                      >  SRR19973063.1461565/1‑150 (MQ=60)
                                              CACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCA                                                                                                     >  SRR19973063.2946927/1‑150 (MQ=60)
                                              CACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCA                                                                                                     >  SRR19973063.2947105/1‑150 (MQ=60)
                                               ACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCAT                                                                                                    <  SRR19973063.4586825/150‑1 (MQ=60)
                                                   CATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCAACACA                                                                                                >  SRR19973063.1353729/1‑150 (MQ=60)
                                                   CATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCGTTTGGAATCGCCATCACA                                                                                                >  SRR19973063.499312/1‑150 (MQ=60)
                                                     TCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACC                                                                                              <  SRR19973063.4164495/150‑1 (MQ=60)
                                                      CACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCC                                                                                             <  SRR19973063.4185484/150‑1 (MQ=60)
                                                       ACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCT                                                                                            <  SRR19973063.1839795/150‑1 (MQ=60)
                                                       ACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCT                                                                                            <  SRR19973063.1839808/150‑1 (MQ=60)
                                                       ACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCT                                                                                            <  SRR19973063.3355649/150‑1 (MQ=60)
                                                        CACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTG                                                                                           <  SRR19973063.2262618/150‑1 (MQ=60)
                                                        CACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTG                                                                                           <  SRR19973063.4364755/150‑1 (MQ=60)
                                                          CCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGAT                                                                                         <  SRR19973063.158015/150‑1 (MQ=60)
                                                          CCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGAT                                                                                         <  SRR19973063.136826/150‑1 (MQ=60)
                                                          CCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGAT                                                                                         <  SRR19973063.136874/150‑1 (MQ=60)
                                                          CCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGAT                                                                                         <  SRR19973063.2496998/150‑1 (MQ=60)
                                                              ATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACT                                                                                     <  SRR19973063.2104389/150‑1 (MQ=60)
                                                                  AGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATC                                                                                 >  SRR19973063.1919393/1‑150 (MQ=60)
                                                                        GTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCAC                                                                           >  SRR19973063.2372857/1‑150 (MQ=60)
                                                                        GTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCCGATGACTGATCACCCAC                                                                           >  SRR19973063.4941198/1‑150 (MQ=60)
                                                                         TTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACG                                                                          <  SRR19973063.3539700/150‑1 (MQ=60)
                                                                             TCCTTTTGGTGCCGGAGATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAA                                                                      <  SRR19973063.4689196/150‑1 (MQ=60)
                                                                              CCTTGTGGTGCCGGATATGCCTTCTCCAGCATAGCTAAGTCCGCTGAAGACAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAAC                                                                     <  SRR19973063.3044647/150‑1 (MQ=60)
                                                                               CTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCGTGATGACTGATCACCCACGCCAACA                                                                    <  SRR19973063.2565844/150‑1 (MQ=60)
                                                                                  TTGGTGCCGGATATGCCTTATCCATCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATA                                                                 <  SRR19973063.4576923/150‑1 (MQ=60)
                                                                                  TTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCTCCGCATTTTGTTGGACATGGGCAATCGGGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATA                                                                 <  SRR19973063.2361961/150‑1 (MQ=60)
                                                                                    GGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACT                                                               >  SRR19973063.1079381/1‑150 (MQ=60)
                                                                                    GGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACT                                                               >  SRR19973063.3830082/1‑150 (MQ=60)
                                                                                      TGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTG                                                             >  SRR19973063.1682298/1‑150 (MQ=60)
                                                                                      TGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTG                                                             >  SRR19973063.4967375/1‑150 (MQ=60)
                                                                                        CCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAACGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTG                                                           >  SRR19973063.740258/1‑150 (MQ=60)
                                                                                        CCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTG                                                           >  SRR19973063.4822907/1‑150 (MQ=60)
                                                                                        CCGGATATGCCTTATCCAGCATAGCTAATACCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGATGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACTCCAACAATACTAGTG                                                           >  SRR19973063.2980224/1‑150 (MQ=60)
                                                                                          GGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCC                                                         <  SRR19973063.4651782/150‑1 (MQ=60)
                                                                                          GGATATGCCTTATCCAGCATAGCTAAGTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCC                                                         <  SRR19973063.4834638/150‑1 (MQ=60)
                                                                                           GATATGCCTTATCCAGCATAGCTAAGTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCG                                                        <  SRR19973063.5159105/150‑1 (MQ=60)
                                                                                             TATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTAAATCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTAGGAATAGCCATCACAACCTGATTACTGATCACCCACGACAACAATACTTGTtcagct                                                      >  SRR19973063.1599253/1‑144 (MQ=60)
                                                                                              ATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGC                                                     >  SRR19973063.1958438/1‑150 (MQ=60)
                                                                                                 CCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGA                                                  <  SRR19973063.4861366/150‑1 (MQ=60)
                                                                                                  CTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGAT                                                 <  SRR19973063.1976839/150‑1 (MQ=60)
                                                                                                        CAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTG                                           <  SRR19973063.393283/150‑1 (MQ=60)
                                                                                                         AGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGA                                          <  SRR19973063.1774639/150‑1 (MQ=60)
                                                                                                         AGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGA                                          <  SRR19973063.1774468/150‑1 (MQ=60)
                                                                                                         AGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGA                                          <  SRR19973063.2185019/150‑1 (MQ=60)
                                                                                                         AGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGA                                          <  SRR19973063.1774580/150‑1 (MQ=60)
                                                                                                           CATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGC                                        >  SRR19973063.4121479/1‑150 (MQ=60)
                                                                                                            ATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCA                                       >  SRR19973063.494009/1‑150 (MQ=60)
                                                                                                              AGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATG                                     <  SRR19973063.2748960/150‑1 (MQ=60)
                                                                                                               GCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGT                                    >  SRR19973063.725287/1‑150 (MQ=60)
                                                                                                               GCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGT                                    <  SRR19973063.1982099/150‑1 (MQ=60)
                                                                                                               GCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGT                                    >  SRR19973063.725377/1‑150 (MQ=60)
                                                                                                                CTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTG                                   <  SRR19973063.1813403/150‑1 (MQ=60)
                                                                                                                 TAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGC                                  >  SRR19973063.4090095/1‑150 (MQ=60)
                                                                                                                 TAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGC                                  >  SRR19973063.4089722/1‑150 (MQ=60)
                                                                                                                  AATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCA                                 >  SRR19973063.4671955/1‑150 (MQ=60)
                                                                                                                     TCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATT                              <  SRR19973063.2908989/150‑1 (MQ=60)
                                                                                                                     TCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATT                              >  SRR19973063.1868995/1‑150 (MQ=60)
                                                                                                                     TCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATT                              <  SRR19973063.883610/150‑1 (MQ=60)
                                                                                                                      CCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTT                             <  SRR19973063.1824527/150‑1 (MQ=60)
                                                                                                                       CGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTC                            <  SRR19973063.3208483/150‑1 (MQ=60)
                                                                                                                         CTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGT                          >  SRR19973063.3023979/1‑150 (MQ=60)
                                                                                                                         CTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAGTTCGT                          >  SRR19973063.3023978/1‑150 (MQ=60)
                                                                                                                          TGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCACTTTCGTT                         >  SRR19973063.2719841/1‑150 (MQ=60)
                                                                                                                          TGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTT                         >  SRR19973063.2986822/1‑150 (MQ=60)
                                                                                                                          TGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTT                         >  SRR19973063.1756597/1‑150 (MQ=60)
                                                                                                                           GAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTG                        >  SRR19973063.1086593/1‑150 (MQ=60)
                                                                                                                             AGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGAC                      >  SRR19973063.2324564/1‑150 (MQ=60)
                                                                                                                             AGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGAC                      <  SRR19973063.3401391/150‑1 (MQ=60)
                                                                                                                             AGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATAACACCCAGAAGACAGAACACCCACGCCAACAAAACGTTTGCCGCGCTGATATTGTGAGCAAGAGCAATATCGTTGAC                      >  SRR19973063.2324576/1‑150 (MQ=60)
                                                                                                                                AAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGACATTGTGAGCATGTGCAATTTCGTTGACTAC                   <  SRR19973063.1393053/150‑1 (MQ=60)
                                                                                                                                 ATTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACC                  >  SRR19973063.4064171/1‑150 (MQ=60)
                                                                                                                                 AGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACC                  <  SRR19973063.4543523/150‑1 (MQ=60)
                                                                                                                                  GTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCG                 >  SRR19973063.3245808/1‑150 (MQ=60)
                                                                                                                                  GTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCG                 >  SRR19973063.1696329/1‑150 (MQ=60)
                                                                                                                                  GTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCG                 >  SRR19973063.1696317/1‑150 (MQ=60)
                                                                                                                                  GTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCG                 <  SRR19973063.2416465/150‑1 (MQ=60)
                                                                                                                                   TTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGC                >  SRR19973063.3783899/1‑150 (MQ=60)
                                                                                                                                    TCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCG               <  SRR19973063.1128960/150‑1 (MQ=60)
                                                                                                                                    TCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCG               >  SRR19973063.795352/1‑150 (MQ=60)
                                                                                                                                    TCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCG               <  SRR19973063.1128946/150‑1 (MQ=60)
                                                                                                                                     CGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGT              >  SRR19973063.3013072/1‑150 (MQ=60)
                                                                                                                                      GACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCGTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTT             >  SRR19973063.4052843/1‑150 (MQ=60)
                                                                                                                                          TCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTT         <  SRR19973063.1251931/150‑1 (MQ=60)
                                                                                                                                            AAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAA       >  SRR19973063.2477691/1‑150 (MQ=60)
                                                                                                                                            AAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAA       >  SRR19973063.2477725/1‑150 (MQ=60)
                                                                                                                                                 CCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCagatcggaagagcacacgtctgaactccagtcacgtagacgtatcgcgtatgccg  >  SRR19973063.963767/1‑95 (MQ=60)
                                                                                                                                                 CCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTC  <  SRR19973063.5140616/150‑1 (MQ=60)
                                                                                                                                                 CCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTC  <  SRR19973063.5140591/150‑1 (MQ=60)
                                                                                                                                                 CCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTC  <  SRR19973063.3948293/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
CGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTC  >  CP009273/1858986‑1859280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑