Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 463,179 C→A 100% I104I (ATC→ATA)  cof → thiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273463,1790CA97.0% 727.3 / 11.1 234I104I (ATC→ATA) cofthiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base A (99/128);  total (103/131)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 243 total aligned reads displayed.

TATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGA  >  CP009273/463034‑463325
                                                                                                                                                 |                                                                                                                                                  
tATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATAtt                                                                                                                                                  >  2:2027908/1‑148 (MQ=255)
tATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATAtt                                                                                                                                                  <  1:1760840/148‑1 (MQ=255)
 aTCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTc                                                                                                                                                 <  1:263536/148‑1 (MQ=255)
   cGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCaa                                                                                                                                               <  2:1999928/148‑1 (MQ=255)
      tGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATga                                                                                                                                            <  2:2727459/148‑1 (MQ=255)
        gATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATgacg                                                                                                                                          <  1:949071/148‑1 (MQ=255)
        gATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATgacg                                                                                                                                          <  1:2642066/148‑1 (MQ=255)
        gATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATgacg                                                                                                                                          <  1:1902441/148‑1 (MQ=255)
        gATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATgacg                                                                                                                                          <  1:2317832/148‑1 (MQ=255)
           gCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGacgacg                                                                                                                                       <  2:6474/148‑1 (MQ=255)
           gCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGacgacg                                                                                                                                       <  2:1787934/148‑1 (MQ=255)
            cGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACgg                                                                                                                                      <  1:351144/148‑1 (MQ=255)
            cGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACgg                                                                                                                                      <  1:350323/148‑1 (MQ=255)
               aTTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggttg                                                                                                                                   <  1:1003306/148‑1 (MQ=255)
               aTTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggttg                                                                                                                                   >  2:663708/1‑148 (MQ=255)
               aTTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggttg                                                                                                                                   >  1:137971/1‑148 (MQ=255)
                tttGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggttgg                                                                                                                                  <  1:800526/148‑1 (MQ=255)
                  tGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggttggtt                                                                                                                                <  2:1610785/148‑1 (MQ=255)
                  tGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggttggtt                                                                                                                                <  2:2713294/148‑1 (MQ=255)
                   gATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGttt                                                                                                                               <  1:1245345/148‑1 (MQ=255)
                    aTTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTa                                                                                                                              <  2:71370/148‑1 (MQ=255)
                    aTTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTa                                                                                                                              >  2:2059858/1‑148 (MQ=255)
                      tACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTAcc                                                                                                                            >  2:2939791/1‑148 (MQ=255)
                       aCCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCg                                                                                                                           <  1:3136030/148‑1 (MQ=255)
                            cAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGaaa                                                                                                                      >  1:1565372/1‑148 (MQ=255)
                             aaCGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAag                                                                                                                     >  1:2407854/1‑148 (MQ=255)
                             aaCGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAag                                                                                                                     <  2:898568/148‑1 (MQ=255)
                              aCGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAaga                                                                                                                    <  2:1479572/148‑1 (MQ=255)
                              aCGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATATACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAaga                                                                                                                    >  1:2876697/1‑148 (MQ=255)
                               cGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAagag                                                                                                                   >  2:168398/1‑148 (MQ=255)
                                ggAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAgaga                                                                                                                  <  1:3236045/148‑1 (MQ=255)
                                  aaCGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATc                                                                                                                >  2:3402312/1‑148 (MQ=255)
                                   aCGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATcc                                                                                                               <  1:1813665/148‑1 (MQ=255)
                                   aCGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATcc                                                                                                               >  1:67980/1‑148 (MQ=255)
                                   aCGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATcc                                                                                                               <  1:2805634/148‑1 (MQ=255)
                                     gcgcgTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCt                                                                                                             >  2:575635/1‑148 (MQ=255)
                                      cgcgTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTg                                                                                                            >  2:1201233/1‑148 (MQ=255)
                                      cgcgTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTg                                                                                                            >  1:489885/1‑148 (MQ=255)
                                      cgcgTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTg                                                                                                            <  1:1705140/148‑1 (MQ=255)
                                         gTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgt                                                                                                         <  2:1085490/148‑1 (MQ=255)
                                          tGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgtt                                                                                                        <  2:1775664/148‑1 (MQ=255)
                                           gCATTCTCTGGAAGGTGAAGTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGAGACCCGAGCCAGCATGCATATATTCAATGCCGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgttg                                                                                                       <  2:3205531/148‑1 (MQ=255)
                                           gCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgttg                                                                                                       >  1:2367919/1‑148 (MQ=255)
                                           gCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgttg                                                                                                       >  2:2786921/1‑148 (MQ=255)
                                           gCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgttg                                                                                                       <  1:2069509/148‑1 (MQ=255)
                                              ttCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGttgttg                                                                                                    <  2:1099249/148‑1 (MQ=255)
                                               tctcTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGc                                                                                                   <  2:3256636/148‑1 (MQ=255)
                                                  ctGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAgg                                                                                                >  2:158609/1‑148 (MQ=255)
                                                    ggAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCa                                                                                              <  1:2027908/148‑1 (MQ=255)
                                                      aaGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCAtt                                                                                            <  2:2232604/148‑1 (MQ=255)
                                                      aaGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCAtt                                                                                            >  2:2136233/1‑148 (MQ=255)
                                                        ggTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTg                                                                                          <  2:1490113/148‑1 (MQ=255)
                                                           gAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCt                                                                                       <  1:3340157/148‑1 (MQ=255)
                                                           gAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCt                                                                                       <  1:1651697/148‑1 (MQ=255)
                                                            aaCTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCta                                                                                      >  1:997206/1‑148 (MQ=255)
                                                             aCTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCtat                                                                                     <  2:648097/148‑1 (MQ=255)
                                                              cTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCtata                                                                                    >  2:3054226/1‑148 (MQ=255)
                                                                 ttACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCg                                                                                 >  1:2933759/1‑148 (MQ=255)
                                                                     aTCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGttt                                                                             >  2:2625176/1‑148 (MQ=255)
                                                                     aTCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGttt                                                                             <  1:1542765/148‑1 (MQ=255)
                                                                         tgatgaTTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGt                                                                         >  1:408386/1‑148 (MQ=255)
                                                                         tgatgaTTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGt                                                                         >  1:411428/1‑148 (MQ=255)
                                                                          gatgatTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGtt                                                                        <  1:3198795/148‑1 (MQ=255)
                                                                          gatgatTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGtt                                                                        >  2:2613332/1‑148 (MQ=255)
                                                                           atgatTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTa                                                                       >  1:3144272/1‑148 (MQ=255)
                                                                           atgatTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTa                                                                       >  1:1478751/1‑148 (MQ=255)
                                                                              atTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCa                                                                    <  2:245067/148‑1 (MQ=255)
                                                                                ttACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGa                                                                  >  1:2108832/1‑148 (MQ=255)
                                                                                ttACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGa                                                                  >  2:864138/1‑148 (MQ=255)
                                                                                   ccTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATaa                                                               <  2:1565372/148‑1 (MQ=255)
                                                                                   ccTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATaa                                                               <  2:3130261/148‑1 (MQ=255)
                                                                                   ccTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATaa                                                               <  2:2971414/148‑1 (MQ=255)
                                                                                     tGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATc                                                             >  2:2233463/1‑148 (MQ=255)
                                                                                     tGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATc                                                             >  2:610769/1‑148 (MQ=255)
                                                                                           tGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTc                                                       >  2:3513680/1‑148 (MQ=255)
                                                                                           tGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTc                                                       >  2:2845433/1‑148 (MQ=255)
                                                                                              cgcgGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCaaa                                                    >  1:360097/1‑148 (MQ=255)
                                                                                                cgGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCaaaaa                                                  >  1:1895295/1‑148 (MQ=255)
                                                                                                  gAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCaaaaaaa                                                >  2:1549534/1‑148 (MQ=255)
                                                                                                  gAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCaaaaaaa                                                >  2:467636/1‑148 (MQ=255)
                                                                                                    gCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATg                                              <  2:1853551/148‑1 (MQ=255)
                                                                                                         tGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACt                                         <  2:2490799/148‑1 (MQ=255)
                                                                                                                    cAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCa                              >  1:2728433/1‑148 (MQ=255)
                                                                                                                      aTGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCAcc                            >  1:249343/1‑148 (MQ=255)
                                                                                                                        gggATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCaa                          >  1:3061676/1‑148 (MQ=255)
                                                                                                                         ggATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAg                         >  1:1946354/1‑148 (MQ=255)
                                                                                                                             aCCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCt                     >  1:91484/1‑148 (MQ=255)
                                                                                                                               ccGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGc                   >  1:3374478/1‑148 (MQ=255)
                                                                                                                               ccGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGc                   >  1:744625/1‑148 (MQ=255)
                                                                                                                                 gAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCtt                 >  2:1050454/1‑148 (MQ=255)
                                                                                                                                  aGCCCGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTc                >  2:786473/1‑148 (MQ=255)
                                                                                                                                  aGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTc                <  1:1046373/148‑1 (MQ=255)
                                                                                                                                     cAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCtgt             >  2:2384943/1‑148 (MQ=255)
                                                                                                                                       gcatgcatATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTgg           >  2:1645172/1‑148 (MQ=255)
                                                                                                                                       gcatgcatATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTgg           <  1:575635/148‑1 (MQ=255)
                                                                                                                                           gcatATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGAt       <  1:2939797/148‑1 (MQ=255)
                                                                                                                                           gcatATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGAGAt       >  2:14724/1‑148 (MQ=255)
                                                                                                                                              tataTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCac    >  2:3158034/1‑148 (MQ=255)
                                                                                                                                              tataTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCac    >  2:1006121/1‑148 (MQ=255)
                                                                                                                                                tCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCacga  >  2:1067661/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGA  >  CP009273/463034‑463325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGAC  >  CP009273/463032‑463326
                                                                                                                                                   |                                                                                                                                                   
GCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATT                                                                                                                                                   <  SRR19973065.1316807/150‑1 (MQ=60)
GCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATT                                                                                                                                                   >  SRR19973065.2959893/1‑150 (MQ=60)
 CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTC                                                                                                                                                  <  SRR19973065.1996205/150‑1 (MQ=60)
 CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTC                                                                                                                                                  <  SRR19973065.2264380/150‑1 (MQ=60)
 CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTC                                                                                                                                                  <  SRR19973065.2264474/150‑1 (MQ=60)
  TATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCA                                                                                                                                                 <  SRR19973065.1772400/150‑1 (MQ=60)
  TATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCA                                                                                                                                                 <  SRR19973065.1772401/150‑1 (MQ=60)
   ATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAA                                                                                                                                                <  SRR19973065.265343/150‑1 (MQ=60)
       CTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGAC                                                                                                                                            <  SRR19973065.746782/150‑1 (MQ=60)
          GATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGAC                                                                                                                                         <  SRR19973065.2332360/150‑1 (MQ=60)
          GATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGAC                                                                                                                                         <  SRR19973065.2658359/150‑1 (MQ=60)
          GATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGAC                                                                                                                                         <  SRR19973065.955344/150‑1 (MQ=60)
          GATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGAC                                                                                                                                         <  SRR19973065.1914944/150‑1 (MQ=60)
          GATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGAC                                                                                                                                         <  SRR19973065.1914938/150‑1 (MQ=60)
          GATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGAC                                                                                                                                         <  SRR19973065.1914911/150‑1 (MQ=60)
          GATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGAC                                                                                                                                         <  SRR19973065.956623/150‑1 (MQ=60)
          GATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGAC                                                                                                                                         <  SRR19973065.2658373/150‑1 (MQ=60)
              CGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTT                                                                                                                                     <  SRR19973065.2133092/150‑1 (MQ=60)
              CGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTT                                                                                                                                     <  SRR19973065.352776/150‑1 (MQ=60)
              CGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTT                                                                                                                                     <  SRR19973065.352788/150‑1 (MQ=60)
              CGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTT                                                                                                                                     <  SRR19973065.353604/150‑1 (MQ=60)
              CGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTT                                                                                                                                     <  SRR19973065.353612/150‑1 (MQ=60)
               GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTG                                                                                                                                    >  SRR19973065.139156/1‑150 (MQ=60)
                 ATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGT                                                                                                                                  <  SRR19973065.1009969/150‑1 (MQ=60)
                  TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTT                                                                                                                                 >  SRR19973065.2067062/1‑150 (MQ=60)
                  TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTT                                                                                                                                 <  SRR19973065.805817/150‑1 (MQ=60)
                   TTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCCGCATGCATATATTCAATGACGACGGTTGGTTT                                                                                                                                >  SRR19973065.2642328/1‑150 (MQ=60)
                     GATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTAC                                                                                                                              <  SRR19973065.1253543/150‑1 (MQ=60)
                        TACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGG                                                                                                                           >  SRR19973065.2791463/1‑150 (MQ=60)
                         ACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGG                                                                                                                          <  SRR19973065.3155106/150‑1 (MQ=60)
                         ACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGG                                                                                                                          <  SRR19973065.3155129/150‑1 (MQ=60)
                            GGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAA                                                                                                                       >  SRR19973065.1575684/1‑150 (MQ=60)
                            GGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAA                                                                                                                       >  SRR19973065.1575773/1‑150 (MQ=60)
                            GGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAA                                                                                                                       >  SRR19973065.1575779/1‑150 (MQ=60)
                             GCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAG                                                                                                                      >  SRR19973065.533562/1‑150 (MQ=60)
                             GCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAG                                                                                                                      >  SRR19973065.2423410/1‑150 (MQ=60)
                             GCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAG                                                                                                                      >  SRR19973065.2423007/1‑150 (MQ=60)
                              CAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATATACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGA                                                                                                                     >  SRR19973065.2894316/1‑150 (MQ=60)
                                  GGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATC                                                                                                                 <  SRR19973065.3255523/150‑1 (MQ=60)
                                   GAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCC                                                                                                                >  SRR19973065.68699/1‑150 (MQ=60)
                                    AACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCC                                                                                                               >  SRR19973065.1003108/1‑150 (MQ=60)
                                     ACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCT                                                                                                              <  SRR19973065.1825607/150‑1 (MQ=60)
                                     ACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCT                                                                                                              <  SRR19973065.2823000/150‑1 (MQ=60)
                                      CGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTG                                                                                                             >  SRR19973065.493192/1‑150 (MQ=60)
                                      CGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTG                                                                                                             >  SRR19973065.493177/1‑150 (MQ=60)
                                      CGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTG                                                                                                             >  SRR19973065.492897/1‑150 (MQ=60)
                                        CGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCG                                                                                                           <  SRR19973065.1716493/150‑1 (MQ=60)
                                           GTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTG                                                                                                        >  SRR19973065.66521/1‑150 (MQ=60)
                                           GTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTG                                                                                                        >  SRR19973065.2384138/1‑150 (MQ=60)
                                           GTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTG                                                                                                        >  SRR19973065.2382677/1‑150 (MQ=60)
                                           GTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTG                                                                                                        >  SRR19973065.1764002/1‑150 (MQ=60)
                                             GCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTT                                                                                                      <  SRR19973065.652068/150‑1 (MQ=60)
                                             GCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTT                                                                                                      <  SRR19973065.2082699/150‑1 (MQ=60)
                                                      GGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATT                                                                                             <  SRR19973065.2040935/150‑1 (MQ=60)
                                                        AAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCATCAATGGGATACCCGAGCCAGCATGCATTTATTCAATGACGACGGTTGGTTTACCGGTAAAGAGTTCCCTGCGTTGTTGCAGGCATTTG                                                                                           <  SRR19973065.3211497/150‑1 (MQ=60)
                                                        AAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTG                                                                                           <  SRR19973065.2891733/150‑1 (MQ=60)
                                                          GGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTC                                                                                         >  SRR19973065.1956409/1‑150 (MQ=60)
                                                           GTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCT                                                                                        <  SRR19973065.1405848/150‑1 (MQ=60)
                                                            TGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTA                                                                                       >  SRR19973065.1003834/1‑150 (MQ=60)
                                                             GAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTAT                                                                                      <  SRR19973065.1662774/150‑1 (MQ=60)
                                                             GAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTAT                                                                                      <  SRR19973065.3408555/150‑1 (MQ=60)
                                                             GAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTAT                                                                                      <  SRR19973065.3360564/150‑1 (MQ=60)
                                                             GAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTAT                                                                                      <  SRR19973065.3360526/150‑1 (MQ=60)
                                                              AACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATA                                                                                     <  SRR19973065.1027672/150‑1 (MQ=60)
                                                              AACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATA                                                                                     <  SRR19973065.2420373/150‑1 (MQ=60)
                                                                 TTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCG                                                                                  >  SRR19973065.2951554/1‑150 (MQ=60)
                                                                   TTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGT                                                                                <  SRR19973065.2920643/150‑1 (MQ=60)
                                                                   TTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGT                                                                                <  SRR19973065.2920752/150‑1 (MQ=60)
                                                                     ACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTT                                                                              <  SRR19973065.1887108/150‑1 (MQ=60)
                                                                       ATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTC                                                                            <  SRR19973065.1552965/150‑1 (MQ=60)
                                                                       ATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTC                                                                            >  SRR19973065.1687975/1‑150 (MQ=60)
                                                                       ATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTC                                                                            <  SRR19973065.380757/150‑1 (MQ=60)
                                                                        TCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCG                                                                           <  SRR19973065.3108491/150‑1 (MQ=60)
                                                                         CGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGT                                                                          >  SRR19973065.414309/1‑150 (MQ=60)
                                                                         CGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGT                                                                          >  SRR19973065.411251/1‑150 (MQ=60)
                                                                         CGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGT                                                                          >  SRR19973065.411243/1‑150 (MQ=60)
                                                                         CGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGT                                                                          >  SRR19973065.410246/1‑150 (MQ=60)
                                                                           TGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTA                                                                        >  SRR19973065.1488203/1‑150 (MQ=60)
                                                                           TGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTA                                                                        >  SRR19973065.3241740/1‑150 (MQ=60)
                                                                           TGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTA                                                                        >  SRR19973065.3163372/1‑150 (MQ=60)
                                                                           TGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGGTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTA                                                                        >  SRR19973065.1488204/1‑150 (MQ=60)
                                                                            GATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTAT                                                                       <  SRR19973065.3218063/150‑1 (MQ=60)
                                                                             ATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGAGACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATC                                                                      <  SRR19973065.2115540/150‑1 (MQ=60)
                                                                               GATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAG                                                                    <  SRR19973065.1628479/150‑1 (MQ=60)
                                                                                ATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGA                                                                   >  SRR19973065.2122395/1‑150 (MQ=60)
                                                                                        GCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGAT                                                           <  SRR19973065.2717633/150‑1 (MQ=60)
                                                                                          GGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGT                                                         >  SRR19973065.1978110/1‑150 (MQ=60)
                                                                                          GGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGT                                                         >  SRR19973065.1978126/1‑150 (MQ=60)
                                                                                           GATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTC                                                        <  SRR19973065.2172270/150‑1 (MQ=60)
                                                                                              GTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAA                                                     >  SRR19973065.362628/1‑150 (MQ=60)
                                                                                                CGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTATCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAA                                                   >  SRR19973065.2806137/1‑150 (MQ=60)
                                                                                                CGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAA                                                   >  SRR19973065.1907749/1‑150 (MQ=60)
                                                                                                CGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAA                                                   <  SRR19973065.1229146/150‑1 (MQ=60)
                                                                                                    GAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATG                                               <  SRR19973065.1265058/150‑1 (MQ=60)
                                                                                                    GAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATG                                               <  SRR19973065.848470/150‑1 (MQ=60)
                                                                                                        TGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCAC                                           <  SRR19973065.3309563/150‑1 (MQ=60)
                                                                                                                    AGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCA                               >  SRR19973065.2745403/1‑150 (MQ=60)
                                                                                                                      CAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACC                             >  SRR19973065.251096/1‑150 (MQ=60)
                                                                                                                        ATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAA                           >  SRR19973065.1781485/1‑150 (MQ=60)
                                                                                                                        ATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAA                           >  SRR19973065.3080395/1‑150 (MQ=60)
                                                                                                                         TGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAG                          >  SRR19973065.1959076/1‑150 (MQ=60)
                                                                                                                             ATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCT                      >  SRR19973065.909741/1‑150 (MQ=60)
                                                                                                                             ATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCT                      >  SRR19973065.92370/1‑150 (MQ=60)
                                                                                                                             ATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCT                      <  SRR19973065.3372869/150‑1 (MQ=60)
                                                                                                                             ATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCT                      >  SRR19973065.92355/1‑150 (MQ=60)
                                                                                                                               ACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGC                    >  SRR19973065.749611/1‑150 (MQ=60)
                                                                                                                               ACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGC                    >  SRR19973065.3395588/1‑150 (MQ=60)
                                                                                                                               ACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGC                    >  SRR19973065.3395116/1‑150 (MQ=60)
                                                                                                                                 CCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTT                  <  SRR19973065.3043501/150‑1 (MQ=60)
                                                                                                                                   GAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCT                <  SRR19973065.2354198/150‑1 (MQ=60)
                                                                                                                                    AGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTG               <  SRR19973065.1053293/150‑1 (MQ=60)
                                                                                                                                         GCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCG          <  SRR19973065.579249/150‑1 (MQ=60)
                                                                                                                                             GCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCA      <  SRR19973065.2957627/150‑1 (MQ=60)
                                                                                                                                             GCATATATTAAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCA      <  SRR19973065.2957633/150‑1 (MQ=60)
                                                                                                                                              CATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCAC     >  SRR19973065.800446/1‑150 (MQ=60)
                                                                                                                                              CATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCAC     >  SRR19973065.2570668/1‑150 (MQ=60)
                                                                                                                                              CATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCAC     >  SRR19973065.1521555/1‑150 (MQ=60)
                                                                                                                                              CATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCAC     >  SRR19973065.3214206/1‑150 (MQ=60)
                                                                                                                                              CATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCAC     >  SRR19973065.1266592/1‑150 (MQ=60)
                                                                                                                                               ATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACG    >  SRR19973065.1315390/1‑150 (MQ=60)
                                                                                                                                                TATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCAATCGGCAGCGTCACCAAGAACTGCTTCTGTGGCGATCACGA   >  SRR19973065.2948689/1‑150 (MQ=60)
                                                                                                                                                 ATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGAC  <  SRR19973065.2485614/150‑1 (MQ=60)
                                                                                                                                                 ATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGAC  <  SRR19973065.1278348/150‑1 (MQ=60)
                                                                                                                                                 ATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGAC  <  SRR19973065.3036135/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
GCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGAC  >  CP009273/463032‑463326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑