Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,680,642 C→A 100% E68D (GAG→GAT)  fumC ← fumarate hydratase (fumarase C),aerobic Class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,680,6420CA98.5% 686.9 / 0.6 217E68D (GAG→GAT) fumCfumarate hydratase (fumarase C),aerobic Class II
Reads supporting (aligned to +/- strand):  ref base C (0/4);  new base A (107/106);  total (107/110)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 219 total aligned reads displayed.

ATTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAG  >  CP009273/1680497‑1680785
                                                                                                                                                 |                                                                                                                                               
  tCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCtt                                                                                                                                             >  2:1449372/1‑148 (MQ=255)
  tCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCtt                                                                                                                                             <  1:706779/148‑1 (MQ=255)
   cACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTc                                                                                                                                            <  1:2603495/148‑1 (MQ=255)
    aCTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGACTGGCGCTCGCTTTATCTTCa                                                                                                                                           >  2:1432328/1‑148 (MQ=255)
      tGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGa                                                                                                                                         >  2:337052/1‑148 (MQ=255)
        gCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGaca                                                                                                                                       <  2:2140495/148‑1 (MQ=255)
         cccGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGacac                                                                                                                                      >  2:2164666/1‑147 (MQ=255)
          ccGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGacaac                                                                                                                                     <  1:2519567/148‑1 (MQ=255)
                aGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCt                                                                                                                               <  2:413513/148‑1 (MQ=255)
                  ccAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTaa                                                                                                                             <  1:824745/148‑1 (MQ=255)
                   cAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTaaa                                                                                                                            <  1:3235568/148‑1 (MQ=255)
                    aGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAAt                                                                                                                           >  2:1766624/1‑148 (MQ=255)
                    aGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAAt                                                                                                                           >  1:1669221/1‑148 (MQ=255)
                    aGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAAt                                                                                                                           <  2:1881986/148‑1 (MQ=255)
                    aGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAAt                                                                                                                           >  1:1292004/1‑148 (MQ=255)
                    aGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCAAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAAt                                                                                                                           >  2:1704531/1‑148 (MQ=255)
                     gCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATc                                                                                                                          <  1:3103456/148‑1 (MQ=255)
                     gCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATc                                                                                                                          >  2:631424/1‑148 (MQ=255)
                     gCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATc                                                                                                                          >  1:433228/1‑148 (MQ=255)
                     gCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATc                                                                                                                          >  2:1935208/1‑148 (MQ=255)
                         ttCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACTAATGGCGCTCGCTTTATCTTCAGACAACAAGACTAAATCTTCa                                                                                                                      >  1:929547/1‑148 (MQ=255)
                         ttCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCa                                                                                                                      >  1:191768/1‑148 (MQ=255)
                         ttCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCa                                                                                                                      <  2:313027/148‑1 (MQ=255)
                           cGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCAtt                                                                                                                    <  2:1881138/148‑1 (MQ=255)
                           cGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCAtt                                                                                                                    >  1:1452733/1‑148 (MQ=255)
                            gttcatgttcatGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTa                                                                                                                   <  2:2990296/148‑1 (MQ=255)
                            gttcatgttcatGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTa                                                                                                                   <  2:1753984/148‑1 (MQ=255)
                            gttcatgttcatGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTa                                                                                                                   <  1:737372/148‑1 (MQ=255)
                             ttcatgttcatgTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTaa                                                                                                                  >  2:2337983/1‑148 (MQ=255)
                             ttcatgttcatgTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTaa                                                                                                                  >  2:134369/1‑148 (MQ=255)
                              tcatgttcatgtTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAAc                                                                                                                 <  1:1771731/148‑1 (MQ=255)
                                 tgttcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACttt                                                                                                              >  1:1994267/1‑148 (MQ=255)
                                  gttcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTATCtttt                                                                                                             >  2:2099249/1‑148 (MQ=255)
                                  gttcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACtttt                                                                                                             >  1:21525/1‑148 (MQ=255)
                                  gttcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACtttt                                                                                                             >  1:3223313/1‑148 (MQ=255)
                                   ttcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTg                                                                                                            >  2:2889988/1‑148 (MQ=255)
                                   ttcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTg                                                                                                            >  2:1563717/1‑148 (MQ=255)
                                   ttcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTg                                                                                                            >  2:87049/1‑148 (MQ=255)
                                    tcttgttACTTTGCGTGCCGGAGCCGGTCTTCCTGATAGCCAGCGGTAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGc                                                                                                           <  2:3497318/145‑1 (MQ=255)
                                    tcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGc                                                                                                           >  2:3290488/1‑148 (MQ=255)
                                    tcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGc                                                                                                           <  1:2530756/148‑1 (MQ=255)
                                      atgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCg                                                                                                         <  1:1001613/148‑1 (MQ=255)
                                           aCTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCa                                                                                                    <  2:88174/148‑1 (MQ=255)
                                             tttGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACg                                                                                                  <  1:1879508/148‑1 (MQ=255)
                                             tttGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACg                                                                                                  >  2:1154688/1‑148 (MQ=255)
                                              ttGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGc                                                                                                 <  1:3474909/148‑1 (MQ=255)
                                              ttGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGc                                                                                                 <  1:1735522/148‑1 (MQ=255)
                                              ttGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGc                                                                                                 <  1:1739111/148‑1 (MQ=255)
                                              ttGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGc                                                                                                 >  1:2825065/1‑148 (MQ=255)
                                                 cGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTg                                                                                              <  1:2293644/148‑1 (MQ=255)
                                                  gTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTgg                                                                                             <  1:674340/148‑1 (MQ=255)
                                                   tGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGt                                                                                            <  2:227163/148‑1 (MQ=255)
                                                   tGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGt                                                                                            <  1:1659946/148‑1 (MQ=255)
                                                   tGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACGTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGt                                                                                            <  1:2939954/148‑1 (MQ=255)
                                                    gCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGtt                                                                                           <  2:1506601/148‑1 (MQ=255)
                                                    gCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGtt                                                                                           >  1:345473/1‑148 (MQ=255)
                                                     ccGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTa                                                                                          <  1:575727/148‑1 (MQ=255)
                                                         aGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAgcgc                                                                                      <  2:629556/148‑1 (MQ=255)
                                                         aGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAgcgc                                                                                      <  1:2562749/148‑1 (MQ=255)
                                                          gCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGcc                                                                                     >  1:1390035/1‑148 (MQ=255)
                                                           ccGGTCTTCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCa                                                                                    >  1:1419168/1‑148 (MQ=255)
                                                                   ccAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGa                                                                            <  1:1450755/148‑1 (MQ=255)
                                                                     aGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAAt                                                                          <  2:1325565/148‑1 (MQ=255)
                                                                      gATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATc                                                                         <  1:2027097/148‑1 (MQ=255)
                                                                        tAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAg                                                                       <  1:1935997/148‑1 (MQ=255)
                                                                         aGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGt                                                                      >  2:405703/1‑148 (MQ=255)
                                                                          gCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTg                                                                     <  1:1578557/148‑1 (MQ=255)
                                                                          gCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTg                                                                     >  1:3335049/1‑148 (MQ=255)
                                                                             aGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAgg                                                                  >  1:1321863/1‑148 (MQ=255)
                                                                              gCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGt                                                                 <  2:2501568/148‑1 (MQ=255)
                                                                                 ggAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTggg                                                              >  2:450792/1‑148 (MQ=255)
                                                                                  gAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGc                                                             <  1:3131330/148‑1 (MQ=255)
                                                                                   aaTTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGACTGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCa                                                            >  2:1838269/1‑148 (MQ=255)
                                                                                         tcgtcATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTtct                                                      >  2:1915828/1‑148 (MQ=255)
                                                                                           gtcATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTcc                                                    <  2:1836063/148‑1 (MQ=255)
                                                                                             cATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGt                                                  >  2:2600164/1‑148 (MQ=255)
                                                                                             cATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGt                                                  <  1:108603/148‑1 (MQ=255)
                                                                                             cATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGt                                                  >  2:103132/1‑148 (MQ=255)
                                                                                              aTGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTc                                                 >  2:1468504/1‑148 (MQ=255)
                                                                                               tGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCg                                                >  2:2960225/1‑148 (MQ=255)
                                                                                                 cTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGaa                                              >  2:758869/1‑148 (MQ=255)
                                                                                                  tGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGaaa                                             >  2:3459090/1‑148 (MQ=255)
                                                                                                    tCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATg                                           >  1:2993823/1‑148 (MQ=255)
                                                                                                        gCCAGTACTTCATCCGCCTCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGa                                       <  1:2787612/148‑1 (MQ=255)
                                                                                                            gTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATg                                   <  1:3469945/148‑1 (MQ=255)
                                                                                                            gTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATg                                   >  1:680135/1‑148 (MQ=255)
                                                                                                             tACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGc                                  >  1:2956263/1‑148 (MQ=255)
                                                                                                               cTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATTTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTc                                >  2:1889209/1‑148 (MQ=255)
                                                                                                               cTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTc                                <  2:2292529/148‑1 (MQ=255)
                                                                                                                  cATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCag                             <  2:1139139/148‑1 (MQ=255)
                                                                                                                   aTCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCagc                            >  2:3225769/1‑148 (MQ=255)
                                                                                                                    tCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCagcg                           <  1:1277536/148‑1 (MQ=255)
                                                                                                                         cgccTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGt                      <  1:3388181/148‑1 (MQ=255)
                                                                                                                             tGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAg                  >  1:1155815/1‑148 (MQ=255)
                                                                                                                              gACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGt                 >  2:533194/1‑148 (MQ=255)
                                                                                                                                cGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGttt               <  2:2722264/148‑1 (MQ=255)
                                                                                                                                    tGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTgc           >  2:358323/1‑148 (MQ=255)
                                                                                                                                    tGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTgc           >  2:2128433/1‑148 (MQ=255)
                                                                                                                                       cgctcgctTTATCTTCAGACAACAAGCCTACATCGTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGcc        <  2:1248289/148‑1 (MQ=255)
                                                                                                                                        gctcgctTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGccc       <  2:1280391/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ATTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAG  >  CP009273/1680497‑1680785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTTATC  >  CP009273/1680497‑1680791
                                                                                                                                                 |                                                                                                                                                     
ATTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTT                                                                                                                                                   <  SRR19973065.175639/150‑1 (MQ=60)
  TCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCA                                                                                                                                                 <  SRR19973065.711491/150‑1 (MQ=60)
   CACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAG                                                                                                                                                <  SRR19973065.2619660/150‑1 (MQ=60)
          CCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAA                                                                                                                                         <  SRR19973065.2535390/150‑1 (MQ=60)
                AGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAA                                                                                                                                   <  SRR19973065.2908429/150‑1 (MQ=60)
                  CCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAAT                                                                                                                                 <  SRR19973065.830154/150‑1 (MQ=60)
                  CCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAAT                                                                                                                                 >  SRR19973065.1680388/1‑150 (MQ=60)
                  CCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAAT                                                                                                                                 >  SRR19973065.1680168/1‑150 (MQ=60)
                  CCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAAT                                                                                                                                 >  SRR19973065.1301526/1‑150 (MQ=60)
                  CCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAAT                                                                                                                                 >  SRR19973065.1300516/1‑150 (MQ=60)
                   CAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATC                                                                                                                                <  SRR19973065.3254848/150‑1 (MQ=60)
                   CAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATC                                                                                                                                >  SRR19973065.31704/1‑150 (MQ=60)
                   CAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATC                                                                                                                                <  SRR19973065.3255041/150‑1 (MQ=60)
                   CAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATC                                                                                                                                >  SRR19973065.436238/1‑150 (MQ=60)
                     GCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTT                                                                                                                              <  SRR19973065.3122393/150‑1 (MQ=60)
                       ACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACTAATGGCGCTCGCTTTATCTTCAGACAACAAGACTAAATCTTCA                                                                                                                            >  SRR19973065.935666/1‑150 (MQ=60)
                       ACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCA                                                                                                                            >  SRR19973065.193267/1‑150 (MQ=60)
                         TTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATT                                                                                                                          >  SRR19973065.1461843/1‑150 (MQ=60)
                         TTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATT                                                                                                                          >  SRR19973065.1462100/1‑150 (MQ=60)
                         TTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATT                                                                                                                          >  SRR19973065.3389229/1‑150 (MQ=60)
                            GTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAAC                                                                                                                       <  SRR19973065.742271/150‑1 (MQ=60)
                            GTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAAC                                                                                                                       <  SRR19973065.742302/150‑1 (MQ=60)
                              TCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTT                                                                                                                     <  SRR19973065.1783328/150‑1 (MQ=60)
                               CATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTT                                                                                                                    >  SRR19973065.2007192/1‑150 (MQ=60)
                                ATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGACTGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTT                                                                                                                   >  SRR19973065.21821/1‑150 (MQ=60)
                                ATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTT                                                                                                                   <  SRR19973065.2696270/150‑1 (MQ=60)
                                ATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTT                                                                                                                   >  SRR19973065.21814/1‑150 (MQ=60)
                                ATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTT                                                                                                                   >  SRR19973065.3242674/1‑150 (MQ=60)
                                ATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTT                                                                                                                   >  SRR19973065.2156615/1‑150 (MQ=60)
                                  GTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGC                                                                                                                 >  SRR19973065.1633902/1‑150 (MQ=60)
                                    TCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCG                                                                                                               <  SRR19973065.2546640/150‑1 (MQ=60)
                                    TCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCG                                                                                                               <  SRR19973065.2545739/150‑1 (MQ=60)
                                     CATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGC                                                                                                              >  SRR19973065.2842182/1‑150 (MQ=60)
                                      ATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCT                                                                                                             <  SRR19973065.1008264/150‑1 (MQ=60)
                                            CTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGC                                                                                                       >  SRR19973065.196512/1‑150 (MQ=60)
                                            CTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGC                                                                                                       >  SRR19973065.2842507/1‑150 (MQ=60)
                                             TTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCT                                                                                                      <  SRR19973065.1891880/150‑1 (MQ=60)
                                              TTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTT                                                                                                     <  SRR19973065.1750599/150‑1 (MQ=60)
                                              TTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTT                                                                                                     <  SRR19973065.1749831/150‑1 (MQ=60)
                                              TTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTT                                                                                                     <  SRR19973065.1747003/150‑1 (MQ=60)
                                              TTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTT                                                                                                     <  SRR19973065.3493248/150‑1 (MQ=60)
                                              TTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTT                                                                                                     <  SRR19973065.3496050/150‑1 (MQ=60)
                                                 CGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGT                                                                                                  <  SRR19973065.2308094/150‑1 (MQ=60)
                                                  GTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTT                                                                                                 >  SRR19973065.347893/1‑150 (MQ=60)
                                                  GTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTT                                                                                                 >  SRR19973065.348182/1‑150 (MQ=60)
                                                  GTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTT                                                                                                 <  SRR19973065.679305/150‑1 (MQ=60)
                                                  GTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTT                                                                                                 <  SRR19973065.678836/150‑1 (MQ=60)
                                                   TGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTA                                                                                                <  SRR19973065.1671065/150‑1 (MQ=60)
                                                   TGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTA                                                                                                <  SRR19973065.3305549/150‑1 (MQ=60)
                                                   TGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACGTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTA                                                                                                <  SRR19973065.2957792/150‑1 (MQ=60)
                                                     CCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGC                                                                                              <  SRR19973065.579434/150‑1 (MQ=60)
                                                     CCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGC                                                                                              <  SRR19973065.579341/150‑1 (MQ=60)
                                                       GGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGC                                                                                            >  SRR19973065.1877541/1‑150 (MQ=60)
                                                        GAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCC                                                                                           >  SRR19973065.1399167/1‑150 (MQ=60)
                                                         AGCCGGTCTTCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCA                                                                                          >  SRR19973065.1428433/1‑150 (MQ=60)
                                                         AGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCA                                                                                          <  SRR19973065.2578768/150‑1 (MQ=60)
                                                              GTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCA                                                                                     <  SRR19973065.1434649/150‑1 (MQ=60)
                                                                CTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATG                                                                                   >  SRR19973065.3224246/1‑150 (MQ=60)
                                                                   CCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAAT                                                                                <  SRR19973065.2681124/150‑1 (MQ=60)
                                                                   CCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAAT                                                                                <  SRR19973065.1460116/150‑1 (MQ=60)
                                                                      GATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAG                                                                             <  SRR19973065.2040121/150‑1 (MQ=60)
                                                                        TAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAACTCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTG                                                                           <  SRR19973065.1947875/150‑1 (MQ=60)
                                                                        TAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTG                                                                           >  SRR19973065.3355408/1‑150 (MQ=60)
                                                                        TAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTG                                                                           >  SRR19973065.136318/1‑150 (MQ=60)
                                                                        GAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTG                                                                           <  SRR19973065.1948670/150‑1 (MQ=60)
                                                                          GCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAG                                                                         <  SRR19973065.2556683/150‑1 (MQ=60)
                                                                          GCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAG                                                                         <  SRR19973065.1589078/150‑1 (MQ=60)
                                                                           CCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGG                                                                        >  SRR19973065.1330611/1‑150 (MQ=60)
                                                                           CCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGG                                                                        >  SRR19973065.1330606/1‑150 (MQ=60)
                                                                           CCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGG                                                                        >  SRR19973065.3087425/1‑150 (MQ=60)
                                                                               CGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGG                                                                    <  SRR19973065.901543/150‑1 (MQ=60)
                                                                                GGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGACTGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGC                                                                   >  SRR19973065.504126/1‑150 (MQ=60)
                                                                                GGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGC                                                                   >  SRR19973065.804224/1‑150 (MQ=60)
                                                                                 GGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCA                                                                  <  SRR19973065.1709666/150‑1 (MQ=60)
                                                                                 GGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCA                                                                  >  SRR19973065.1858279/1‑150 (MQ=60)
                                                                                  GAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCAT                                                                 <  SRR19973065.3150380/150‑1 (MQ=60)
                                                                                   AATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATT                                                                >  SRR19973065.2600227/1‑150 (MQ=60)
                                                                                     TTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTT                                                              >  SRR19973065.3498604/1‑150 (MQ=60)
                                                                                             CATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCG                                                      <  SRR19973065.109589/150‑1 (MQ=60)
                                                                                             CATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCG                                                      <  SRR19973065.1897424/150‑1 (MQ=60)
                                                                                                  TGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATG                                                 >  SRR19973065.3012158/1‑150 (MQ=60)
                                                                                                       TGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAA                                            >  SRR19973065.1422519/1‑150 (MQ=60)
                                                                                                        GCCAGTACTTCATCCGCCTCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAA                                           <  SRR19973065.2804895/150‑1 (MQ=60)
                                                                                                         CCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAAT                                          >  SRR19973065.1536084/1‑150 (MQ=60)
                                                                                                          CAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATG                                         <  SRR19973065.339408/150‑1 (MQ=60)
                                                                                                          CAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATG                                         >  SRR19973065.684679/1‑150 (MQ=60)
                                                                                                          CAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATG                                         >  SRR19973065.684664/1‑150 (MQ=60)
                                                                                                           AGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGC                                        >  SRR19973065.2973899/1‑150 (MQ=60)
                                                                                                           AGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGC                                        >  SRR19973065.2974300/1‑150 (MQ=60)
                                                                                                           AGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGC                                        >  SRR19973065.2974302/1‑150 (MQ=60)
                                                                                                            GTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCT                                       <  SRR19973065.3491066/150‑1 (MQ=60)
                                                                                                              ACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCC                                     <  SRR19973065.3094242/150‑1 (MQ=60)
                                                                                                               CTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCA                                    <  SRR19973065.2200011/150‑1 (MQ=60)
                                                                                                                TTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAG                                   <  SRR19973065.635532/150‑1 (MQ=60)
                                                                                                                    TCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAG                               <  SRR19973065.1286140/150‑1 (MQ=60)
                                                                                                                    TCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAG                               <  SRR19973065.1285969/150‑1 (MQ=60)
                                                                                                                     CCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGC                              >  SRR19973065.1207909/1‑150 (MQ=60)
                                                                                                                         CGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTG                          <  SRR19973065.1661121/150‑1 (MQ=60)
                                                                                                                         CGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTG                          <  SRR19973065.3408901/150‑1 (MQ=60)
                                                                                                                           CCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAG                        >  SRR19973065.1163238/1‑150 (MQ=60)
                                                                                                                             TGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTT                      >  SRR19973065.3462764/1‑150 (MQ=60)
                                                                                                                                    TGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGC               >  SRR19973065.1161735/1‑150 (MQ=60)
                                                                                                                                           CGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAG        <  SRR19973065.2026347/150‑1 (MQ=60)
                                                                                                                                           CGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAG        <  SRR19973065.243048/150‑1 (MQ=60)
                                                                                                                                           CGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAG        <  SRR19973065.1035119/150‑1 (MQ=60)
                                                                                                                                            GCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGC       <  SRR19973065.929644/150‑1 (MQ=60)
                                                                                                                                                TATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGTATCAGTGAGGTGGGCATTTTCTCCGTCGTAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTTAT   <  SRR19973065.1144087/150‑1 (MQ=60)
                                                                                                                                                TATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTTAT   <  SRR19973065.2578943/150‑1 (MQ=60)
                                                                                                                                                TATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTTAT   >  SRR19973065.908928/1‑150 (MQ=60)
                                                                                                                                                TATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTTAT   <  SRR19973065.87893/150‑1 (MQ=60)
                                                                                                                                                TATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTTAT   <  SRR19973065.2907651/150‑1 (MQ=60)
                                                                                                                                                 ATCTTCAGACAACAATCCTAAATCTTCATTAACTTTTGCCGGTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTTGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTTATC  <  SRR19973065.953793/150‑1 (MQ=60)
                                                                                                                                                 |                                                                                                                                                     
ATTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTTATC  >  CP009273/1680497‑1680791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑