Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,829,319 C→A 100% I85I (ATC→ATA)  ydjZ → TVP38/TMEM64 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,829,3190CA99.0% 640.9 / ‑2.0 201I85I (ATC→ATA) ydjZTVP38/TMEM64 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (81/117);  total (84/117)
Fisher's exact test for biased strand distribution p-value = 1.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 201 total aligned reads displayed.

CAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGT  >  CP009273/1829175‑1829462
                                                                                                                                                |                                                                                                                                               
cAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAAcc                                                                                                                                              <  2:46767/148‑1 (MQ=255)
 aaTCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCt                                                                                                                                             >  1:3089095/1‑148 (MQ=255)
 aaTCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCt                                                                                                                                             <  2:1765159/148‑1 (MQ=255)
  aTCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCtt                                                                                                                                            <  1:3303835/148‑1 (MQ=255)
  aTCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCtt                                                                                                                                            <  1:1064072/148‑1 (MQ=255)
    cGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTg                                                                                                                                          <  2:2944979/148‑1 (MQ=255)
       aGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCGGCGTTTTTGATAAACATTGCCa                                                                                                                                       >  1:1004335/1‑148 (MQ=255)
       aGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTAAGTCTTAAGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCa                                                                                                                                       >  1:1004305/1‑148 (MQ=255)
        gCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCaa                                                                                                                                      <  2:1130551/148‑1 (MQ=255)
         cGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAAt                                                                                                                                     >  2:537141/1‑148 (MQ=255)
          gTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATg                                                                                                                                    <  1:1356538/148‑1 (MQ=255)
           ttGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTGCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGc                                                                                                                                   >  2:2633519/1‑148 (MQ=255)
            tGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCg                                                                                                                                  <  1:929784/148‑1 (MQ=255)
             gcggcgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGt                                                                                                                                 <  2:1906391/148‑1 (MQ=255)
             gcggcgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGt                                                                                                                                 <  2:1870809/148‑1 (MQ=255)
             gcggcgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGt                                                                                                                                 <  2:131049/148‑1 (MQ=255)
                gcgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGc                                                                                                                              <  2:2565158/148‑1 (MQ=255)
                 cgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCt                                                                                                                             <  2:1547441/148‑1 (MQ=255)
                  gTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTg                                                                                                                            <  2:1035236/148‑1 (MQ=255)
                    ttGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATGACCTTTGCCAATGCGTCGCTGtt                                                                                                                          >  1:1241657/1‑148 (MQ=255)
                    ttGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGtt                                                                                                                          <  2:2903871/148‑1 (MQ=255)
                    ttGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGGTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGtt                                                                                                                          >  1:2458105/1‑148 (MQ=255)
                     tGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGttt                                                                                                                         <  2:1971223/148‑1 (MQ=255)
                          ccgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGgcgc                                                                                                                    <  1:1830268/148‑1 (MQ=255)
                                  cAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTggg                                                                                                            >  1:3410400/1‑148 (MQ=255)
                                  cAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTggg                                                                                                            >  1:2284450/1‑148 (MQ=255)
                                    aCAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGggc                                                                                                          >  2:2929766/1‑148 (MQ=255)
                                    aCAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGggc                                                                                                          >  1:2275840/1‑148 (MQ=255)
                                        ggTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCt                                                                                                      >  2:172176/1‑148 (MQ=255)
                                         gTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCtt                                                                                                     <  1:1876089/148‑1 (MQ=255)
                                         gTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCtt                                                                                                     <  1:2654874/148‑1 (MQ=255)
                                           ataGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGc                                                                                                   <  2:2843377/148‑1 (MQ=255)
                                              gAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATTCGTCGCTGTTTGTCGCGTTCTGGGGCGGCTTGCTGt                                                                                                >  1:1446790/1‑148 (MQ=255)
                                               aaCGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTc                                                                                               >  2:2745201/1‑148 (MQ=255)
                                                   cTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTgg                                                                                           >  2:1660117/1‑148 (MQ=255)
                                                   cTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTgg                                                                                           >  2:2457241/1‑148 (MQ=255)
                                                   cTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTgg                                                                                           >  1:3503841/1‑148 (MQ=255)
                                                    tttATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGa                                                                                          <  1:3153668/148‑1 (MQ=255)
                                                     ttATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGAc                                                                                         >  1:2341922/1‑148 (MQ=255)
                                                      tATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGAcc                                                                                        <  1:2604619/148‑1 (MQ=255)
                                                      tATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGAcc                                                                                        <  1:367343/148‑1 (MQ=255)
                                                       aTTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCa                                                                                       <  2:2797174/148‑1 (MQ=255)
                                                        ttCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGTGCGGCTTGCTGTCGTGGACCAg                                                                                      <  2:386164/148‑1 (MQ=255)
                                                        ttCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAg                                                                                      <  1:280619/148‑1 (MQ=255)
                                                         tCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGt                                                                                     <  1:1694506/148‑1 (MQ=255)
                                                          cAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGtt                                                                                    >  1:2955561/1‑148 (MQ=255)
                                                             tCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGa                                                                                 >  1:3121810/1‑148 (MQ=255)
                                                             tCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGa                                                                                 >  1:2941603/1‑148 (MQ=255)
                                                              cTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGAt                                                                                <  1:2288451/148‑1 (MQ=255)
                                                                tACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATgg                                                                              >  2:690648/1‑148 (MQ=255)
                                                                 aCGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGc                                                                             <  2:2133152/148‑1 (MQ=255)
                                                                 aCGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGc                                                                             <  1:1047388/148‑1 (MQ=255)
                                                                            gcggcAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCggcgcggcgc                                                                  >  2:2641969/1‑148 (MQ=255)
                                                                              ggcAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCtg                                                                <  2:626097/148‑1 (MQ=255)
                                                                              ggcAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCtg                                                                <  2:2391122/148‑1 (MQ=255)
                                                                                cAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCtgtg                                                              <  2:772182/148‑1 (MQ=255)
                                                                                   ttGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCtt                                                           <  2:3089095/148‑1 (MQ=255)
                                                                                      tctcGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCttttt                                                        <  1:1617900/148‑1 (MQ=255)
                                                                                        tcGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTa                                                      >  2:2133981/1‑148 (MQ=255)
                                                                                        tcGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGACGCTGTGCTTTTTTa                                                      >  2:3153116/1‑148 (MQ=255)
                                                                                         cGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggcgcggcg                                                                   <  2:3391124/134‑1 (MQ=255)
                                                                                         cGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGTGCTGTGCTTTTTTAt                                                     <  1:68718/148‑1 (MQ=255)
                                                                                         cGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTAt                                                     <  1:68478/148‑1 (MQ=255)
                                                                                         cGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGAGCTGTGCTTTTTTAt                                                     >  2:2359063/1‑148 (MQ=255)
                                                                                           ttCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCg                                                   >  1:1181712/1‑148 (MQ=255)
                                                                                           ttCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCg                                                   >  1:556064/1‑148 (MQ=255)
                                                                                                  tgatgaTTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGt                                            >  2:2748533/1‑148 (MQ=255)
                                                                                                   gatgatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTg                                           >  1:231432/1‑148 (MQ=255)
                                                                                                    atgatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGa                                          <  2:3410400/148‑1 (MQ=255)
                                                                                                    atgatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGa                                          <  2:2284450/148‑1 (MQ=255)
                                                                                                    atgatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGAGCTGTGCTTTTTTATCGCCAGAGTGa                                          <  2:522154/148‑1 (MQ=255)
                                                                                                        ttttGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATggg                                      <  2:149922/148‑1 (MQ=255)
                                                                                                         tttGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGGCGGCGCGGCGCTGTGGTTTTTTATAGCCAGAGTGATGGGc                                     >  1:3325647/1‑148 (MQ=255)
                                                                                                         tttGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCGGGGCTTTTTTATCGCCAGAGGGATGGGc                                     >  1:3326296/1‑148 (MQ=255)
                                                                                                               ggCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGaa                               >  2:1537194/1‑148 (MQ=255)
                                                                                                                gCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAg                              <  1:3205166/148‑1 (MQ=255)
                                                                                                                gCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAg                              <  1:2633519/148‑1 (MQ=255)
                                                                                                                gCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAg                              >  2:2595241/1‑148 (MQ=255)
                                                                                                                 ccATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgt                             >  1:2022312/1‑148 (MQ=255)
                                                                                                                  cATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtg                            <  1:537141/148‑1 (MQ=255)
                                                                                                                   aTTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtgg                           >  2:2753079/1‑148 (MQ=255)
                                                                                                                   aTTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGTGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtgg                           >  2:425474/1‑148 (MQ=255)
                                                                                                                   aTTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGGCGGCGCGGCGCTGTGCTTTTTTTTAGCCAGAGTGTTGGGCCGCGAAgtgg                           >  1:2405430/1‑148 (MQ=255)
                                                                                                                   aTTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtgg                           >  1:445522/1‑148 (MQ=255)
                                                                                                                    gcgctgcACCGCTACCTGCGTTTTTGAGAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtggt                          <  1:797250/146‑1 (MQ=255)
                                                                                                                     tgctgcACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtggtg                         >  2:1699169/1‑148 (MQ=255)
                                                                                                                     tgctgcACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtggtg                         >  1:2868444/1‑148 (MQ=255)
                                                                                                                      gctgcACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGtggtgg                        <  2:2038019/148‑1 (MQ=255)
                                                                                                                            ccGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAAt                  <  2:1004333/148‑1 (MQ=255)
                                                                                                                             cGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGCAGTGGTGGAAAAAtt                 >  1:2247499/1‑148 (MQ=255)
                                                                                                                             cGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGCGGCGGCTTTCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCAGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAAtt                 >  2:45314/1‑148 (MQ=255)
                                                                                                                                tACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAAc              <  1:1795844/148‑1 (MQ=255)
                                                                                                                                 aCCTGCGTTTTTGATAACCTTTGCCACTGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGTCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAAcc             <  2:3016615/148‑1 (MQ=255)
                                                                                                                                     gCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCa         >  2:1731784/1‑148 (MQ=255)
                                                                                                                                     gCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGACGTGGACCAGTTCGATGGCAGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGCAGTGGTGGAAAATTTCTCCGGCa         >  2:2307837/1‑148 (MQ=255)
                                                                                                                                         ttttGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAAc     <  1:1969598/148‑1 (MQ=255)
                                                                                                                                         ttttGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAAc     <  1:277577/148‑1 (MQ=255)
                                                                                                                                          tttGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAAcc    <  2:3254109/148‑1 (MQ=255)
                                                                                                                                          tttGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAAcc    <  2:1451529/148‑1 (MQ=255)
                                                                                                                                            tGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGt  >  1:2007056/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGT  >  CP009273/1829175‑1829462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTG  >  CP009273/1829174‑1829467
                                                                                                                                                 |                                                                                                                                                    
TCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACCGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCT                                                                                                                                                  >  SRR19973065.1350855/1‑150 (MQ=60)
TCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCT                                                                                                                                                  >  SRR19973065.3107956/1‑150 (MQ=60)
  AATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTT                                                                                                                                                <  SRR19973065.2513441/150‑1 (MQ=60)
   ATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTG                                                                                                                                               <  SRR19973065.3323959/150‑1 (MQ=60)
   ATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTG                                                                                                                                               <  SRR19973065.1071124/150‑1 (MQ=60)
      GCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTTCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAAACTTTTCCA                                                                                                                                            >  SRR19973065.1011002/1‑150 (MQ=60)
      GCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCGGCGTTTTTGATAAACATTGCCA                                                                                                                                            >  SRR19973065.1011004/1‑150 (MQ=60)
      GCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTAAGTCTTAAGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAAACTTTTCCA                                                                                                                                            >  SRR19973065.1010974/1‑150 (MQ=60)
           GTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCG                                                                                                                                       <  SRR19973065.1365527/150‑1 (MQ=60)
             TGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTC                                                                                                                                     <  SRR19973065.935903/150‑1 (MQ=60)
                GGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCT                                                                                                                                  <  SRR19973065.2005426/150‑1 (MQ=60)
                 GCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTG                                                                                                                                 <  SRR19973065.3020449/150‑1 (MQ=60)
                   GTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATGACCTTTGCCAATGCGTCGCTGTT                                                                                                                               >  SRR19973065.1249823/1‑150 (MQ=60)
                   GTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGGTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTT                                                                                                                               >  SRR19973065.2473598/1‑150 (MQ=60)
                           CCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGT                                                                                                                       <  SRR19973065.1842325/150‑1 (MQ=60)
                              TGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACATATGACAAAGAGTCGCTGTTTGGCGCGTTCT                                                                                                                    >  SRR19973065.2817320/1‑150 (MQ=60)
                                 ACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTTGCGCGTTCTGGG                                                                                                                 >  SRR19973065.3431237/1‑150 (MQ=60)
                                 ACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGG                                                                                                                 <  SRR19973065.1435621/150‑1 (MQ=60)
                                 ACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGG                                                                                                                 >  SRR19973065.2298873/1‑150 (MQ=60)
                                 ACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGG                                                                                                                 >  SRR19973065.2298851/1‑150 (MQ=60)
                                   CAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGC                                                                                                               >  SRR19973065.2290200/1‑150 (MQ=60)
                                        GGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTT                                                                                                          >  SRR19973065.588671/1‑150 (MQ=60)
                                          GTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGC                                                                                                        <  SRR19973065.1888442/150‑1 (MQ=60)
                                          GTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGC                                                                                                        <  SRR19973065.2671276/150‑1 (MQ=60)
                                            ATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTG                                                                                                      >  SRR19973065.361523/1‑150 (MQ=60)
                                             TAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATTCGTCGCTGTTTGTCGCGTTCTGGGGCGGCTTGCTGT                                                                                                     >  SRR19973065.1456142/1‑150 (MQ=60)
                                                AACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGT                                                                                                  >  SRR19973065.2343617/1‑150 (MQ=60)
                                                 ACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTG                                                                                                 <  SRR19973065.2889186/150‑1 (MQ=60)
                                                  CGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGG                                                                                                >  SRR19973065.3525116/1‑150 (MQ=60)
                                                    CTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGAC                                                                                              >  SRR19973065.2356540/1‑150 (MQ=60)
                                                     TTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACC                                                                                             <  SRR19973065.3172799/150‑1 (MQ=60)
                                                       TATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAG                                                                                           <  SRR19973065.2620788/150‑1 (MQ=60)
                                                       TATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAG                                                                                           <  SRR19973065.369914/150‑1 (MQ=60)
                                                         TTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTT                                                                                         <  SRR19973065.282752/150‑1 (MQ=60)
                                                         TTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTT                                                                                         <  SRR19973065.282479/150‑1 (MQ=60)
                                                         TTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGGTTTGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCACTT                                                                                         >  SRR19973065.2973596/1‑150 (MQ=60)
                                                          TCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTC                                                                                        <  SRR19973065.1705819/150‑1 (MQ=60)
                                                           CAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCG                                                                                       <  SRR19973065.91166/150‑1 (MQ=60)
                                                            AGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGA                                                                                      >  SRR19973065.3140821/1‑150 (MQ=60)
                                                            AGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGA                                                                                      >  SRR19973065.2959461/1‑150 (MQ=60)
                                                            AGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGA                                                                                      >  SRR19973065.429309/1‑150 (MQ=60)
                                                               CTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGG                                                                                   <  SRR19973065.1966216/150‑1 (MQ=60)
                                                               CTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGG                                                                                   <  SRR19973065.2302870/150‑1 (MQ=60)
                                                               CTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGG                                                                                   <  SRR19973065.2302877/150‑1 (MQ=60)
                                                                 TACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCC                                                                                 <  SRR19973065.1114212/150‑1 (MQ=60)
                                                                  ACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCG                                                                                <  SRR19973065.2968135/150‑1 (MQ=60)
                                                                  ACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCG                                                                                <  SRR19973065.1054316/150‑1 (MQ=60)
                                                                  ACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCG                                                                                <  SRR19973065.1054315/150‑1 (MQ=60)
                                                                  ACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCG                                                                                <  SRR19973065.549652/150‑1 (MQ=60)
                                                                  ACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCG                                                                                <  SRR19973065.1054277/150‑1 (MQ=60)
                                                                      CGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGTTAACCTTTGCCAATGCGTCGCTGTTTGGGGCGGTCTGGGGCGGCTTGCgggcgtgggccagggggtggggcggggc                                                                            >  SRR19973065.1514827/1‑122 (MQ=60)
                                                                              CGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCCGTTCGATGGCCGGCGCGGCGCGGT                                                                    >  SRR19973065.2727313/1‑150 (MQ=60)
                                                                                    TTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTT                                                              >  SRR19973065.2472360/1‑150 (MQ=60)
                                                                                    TTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTT                                                              >  SRR19973065.3293004/1‑150 (MQ=60)
                                                                                      GTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTT                                                            <  SRR19973065.472995/150‑1 (MQ=60)
                                                                                       TCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTA                                                           <  SRR19973065.1628754/150‑1 (MQ=60)
                                                                                        CTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTagatcggaagag                                                          >  SRR19973065.3411868/1‑138 (MQ=60)
                                                                                          CGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGTGCTGTGCTTTTTTATCG                                                        <  SRR19973065.69448/150‑1 (MQ=60)
                                                                                          CGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCG                                                        >  SRR19973065.559616/1‑150 (MQ=60)
                                                                                          CGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCG                                                        <  SRR19973065.1869901/150‑1 (MQ=60)
                                                                                          CGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCG                                                        <  SRR19973065.69205/150‑1 (MQ=60)
                                                                                          CGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCG                                                        <  SRR19973065.69433/150‑1 (MQ=60)
                                                                                          CGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCG                                                        >  SRR19973065.1189197/1‑150 (MQ=60)
                                                                                                  TTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTG                                                >  SRR19973065.233114/1‑150 (MQ=60)
                                                                                                   TGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGGTGTCGTGGACCAGGTCGATGGCCGGCGCGGCGCTGTGGTTTTTTATAGCCAGAGTGA                                               >  SRR19973065.694747/1‑150 (MQ=60)
                                                                                                        ATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCCGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGC                                          >  SRR19973065.3345931/1‑150 (MQ=60)
                                                                                                        ATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGGCGGCGCGGCGCTGTGGTTTTTTATAGCCAGAGTGATGGGC                                          >  SRR19973065.3345941/1‑150 (MQ=60)
                                                                                                        ATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGGGTTGGGC                                          >  SRR19973065.3345983/1‑150 (MQ=60)
                                                                                                        ATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCGGGGCTTTTTTATCGCCAGAGGGATGGGC                                          >  SRR19973065.3346591/1‑150 (MQ=60)
                                                                                                          TTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCG                                        <  SRR19973065.2630305/150‑1 (MQ=60)
                                                                                                               AGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAG                                   <  SRR19973065.1214503/150‑1 (MQ=60)
                                                                                                                GGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGT                                  >  SRR19973065.2035320/1‑150 (MQ=60)
                                                                                                                GGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCGGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGT                                  >  SRR19973065.2035297/1‑150 (MQ=60)
                                                                                                                 GCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTG                                 <  SRR19973065.3224459/150‑1 (MQ=60)
                                                                                                                 GCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTG                                 <  SRR19973065.2649794/150‑1 (MQ=60)
                                                                                                                 GCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTG                                 <  SRR19973065.2650480/150‑1 (MQ=60)
                                                                                                                 GCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTG                                 <  SRR19973065.2292554/150‑1 (MQ=60)
                                                                                                                  CCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCCGTTCGATGGCCGGCGCGGCGCTGTGGTTTTTTATAGCCAGAGTGATGGGCCGCGAAGTGG                                >  SRR19973065.2420533/1‑150 (MQ=60)
                                                                                                                  CCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGGCGGCGCGGCGCTGTGCTTTTTTTTAGCCAGAGTGTTGGGCCGCGAAGTGG                                >  SRR19973065.2420563/1‑150 (MQ=60)
                                                                                                                  CCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCGGTGGTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGG                                >  SRR19973065.448607/1‑150 (MQ=60)
                                                                                                                   CATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGT                               <  SRR19973065.315745/150‑1 (MQ=60)
                                                                                                                   CATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGT                               <  SRR19973065.1813651/150‑1 (MQ=60)
                                                                                                                   CATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGT                               <  SRR19973065.3482621/150‑1 (MQ=60)
                                                                                                                   CATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGT                               >  SRR19973065.2264789/1‑150 (MQ=60)
                                                                                                                   CATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGT                               <  SRR19973065.540639/150‑1 (MQ=60)
                                                                                                                    ATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTG                              >  SRR19973065.2886039/1‑150 (MQ=60)
                                                                                                                     gcGCTGCACCGCTACCTGCGTTTTTGAGAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGG                             <  SRR19973065.802519/148‑1 (MQ=60)
                                                                                                                            ACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGCAGTGGTGGAAAAATT                      >  SRR19973065.2261756/1‑150 (MQ=60)
                                                                                                                            ACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATT                      <  SRR19973065.1551154/150‑1 (MQ=60)
                                                                                                                             CCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTA                     <  SRR19973065.2053064/150‑1 (MQ=60)
                                                                                                                                 TACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCG                 <  SRR19973065.1745651/150‑1 (MQ=60)
                                                                                                                                 TACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCG                 <  SRR19973065.1807631/150‑1 (MQ=60)
                                                                                                                                  ACCTGCGTTTTTGATAACCATTTCCAATTCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCACTTAGATTGCCCGCGCGGCGCGGTGGTTTTTTATAGCCACAGTGATttgcccccaacaggtggaaaaaataaaccg                >  SRR19973065.3289318/1‑120 (MQ=60)
                                                                                                                                      GCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAA            <  SRR19973065.318157/150‑1 (MQ=60)
                                                                                                                                         TTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACC         <  SRR19973065.3129156/150‑1 (MQ=60)
                                                                                                                                          TTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCG        <  SRR19973065.279431/150‑1 (MQ=60)
                                                                                                                                          TTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCG        <  SRR19973065.1982442/150‑1 (MQ=60)
                                                                                                                                           TTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGT       <  SRR19973065.1703175/150‑1 (MQ=60)
                                                                                                                                           TTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGT       >  SRR19973065.2020019/1‑150 (MQ=60)
                                                                                                                                              GATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCT    >  SRR19973065.1138105/1‑150 (MQ=60)
                                                                                                                                                TAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCGTGCTGTCGTGGACCAGTTCGTTGGCCGGGCCGGCGCTGTGCTTTTTTATAGCCACTGTTATGGGCCGCGAAGTTGTGGTCAAAATAACACGCAAAACCGTGCTTT  >  SRR19973065.402139/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                                    
TCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTG  >  CP009273/1829174‑1829467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑