Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,839,854 C→A 93.9% R455L (CGC→CTC)  topB ← DNA topoisomerase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,839,8540CA93.9% 514.8 / 20.6 213R455L (CGC→CTC) topBDNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base C (9/6);  new base A (83/114);  total (93/120)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

GATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGG  >  CP009273/1839709‑1839998
                                                                                                                                                 |                                                                                                                                                
gctCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCGTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCgagc                                                                                                                                                <  2:2124891/146‑1 (MQ=255)
gATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCgagc                                                                                                                                                <  2:2801991/148‑1 (MQ=255)
 aTCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGct                                                                                                                                               <  2:869516/148‑1 (MQ=255)
 aTCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATAGAGct                                                                                                                                               >  1:2013542/1‑148 (MQ=255)
  tCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGctc                                                                                                                                              >  2:3208720/1‑148 (MQ=255)
  tCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGAGctc                                                                                                                                              >  2:1138729/1‑148 (MQ=255)
   cccGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGctct                                                                                                                                             <  1:1859775/148‑1 (MQ=255)
      ggTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCCTCGCGCTCGTtg                                                                                                                                          >  2:2414314/1‑148 (MQ=255)
       gTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTtgc                                                                                                                                         <  2:1848328/148‑1 (MQ=255)
         cATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTtgctg                                                                                                                                       >  2:484063/1‑148 (MQ=255)
         cATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTtgctg                                                                                                                                       <  1:2455259/148‑1 (MQ=255)
           tCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGcc                                                                                                                                     <  2:1459938/148‑1 (MQ=255)
                gAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACa                                                                                                                                >  2:1041704/1‑148 (MQ=255)
                gAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACa                                                                                                                                >  2:1399561/1‑148 (MQ=255)
                 aaaGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAg                                                                                                                               >  2:2457842/1‑148 (MQ=255)
                 aaaGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAg                                                                                                                               >  2:2826498/1‑148 (MQ=255)
                 aaaGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGCGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCATCGCGCTCTTTGGTGCCTAACac                                                                                                                               >  1:2989562/1‑147 (MQ=255)
                   aGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTAATCGAGCTCTTTGGTGCCTAACAGCg                                                                                                                             >  2:2664585/1‑148 (MQ=255)
                    gCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGt                                                                                                                            >  2:1689645/1‑148 (MQ=255)
                    gCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCGTTGCTGCCTAACAGCGt                                                                                                                            >  2:2053307/1‑148 (MQ=255)
                    gCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATAGAGCTCTTTGCTGCCTAACAGCGt                                                                                                                            >  2:2053412/1‑148 (MQ=255)
                    gCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTTAATCGAGCTCGTTGGGGGCGAACAGCgg                                                                                                                            >  1:2384968/1‑147 (MQ=255)
                    gCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGAGCGCGTTGCTGCCGAACAGCGt                                                                                                                            >  2:1731607/1‑148 (MQ=255)
                       gtgtTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCAAAGAGCTCATTGGTGGCACAAAGCGTgag                                                                                                                         >  1:1927409/1‑146 (MQ=255)
                        tgtTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTGATCGAGCTCTTTGCTGGCGAACAGCGTgcgc                                                                                                                        >  1:1071080/1‑148 (MQ=255)
                        tgtTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTTCTGCCGAAAAGCGTgcgc                                                                                                                        >  1:2735831/1‑148 (MQ=255)
                         gtTGCATCGGTAAACTGGCGCGGCGGCTGGGTGTGCCGCTCTACCACTTCACCTTGTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGcc                                                                                                                       <  2:2403200/148‑1 (MQ=255)
                         gtTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGcc                                                                                                                       <  2:2403100/148‑1 (MQ=255)
                               tCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCGTTGCTGCCTAACAGCGTGCGCCAGCCTg                                                                                                                 >  2:2921416/1‑148 (MQ=255)
                               tCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGGTGTTCATCGAGGTCTTTGCTGCCTAACAGCGTGCGCCAGCCTg                                                                                                                 >  1:2532375/1‑148 (MQ=255)
                                 ggTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCt                                                                                                               <  2:3469358/148‑1 (MQ=255)
                                 ggTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCt                                                                                                               <  1:1843121/148‑1 (MQ=255)
                                     aaaTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAg                                                                                                           <  1:284575/148‑1 (MQ=255)
                                     aaaTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAAAAGCGTGCGCCAGCCTGCTTAAg                                                                                                           >  2:3295681/1‑148 (MQ=255)
                                     aaaTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTTTTGATGGAGCTCTTTTGGGCCAAACAGCGGGCGCCCGCCCGCGTCAg                                                                                                           >  1:3267726/1‑148 (MQ=255)
                                       aTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCa                                                                                                         >  2:3440546/1‑148 (MQ=255)
                                       aTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCAGCTTCAGCa                                                                                                         >  2:2596853/1‑148 (MQ=255)
                                       aTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCAGCTTCAGCa                                                                                                         >  2:558592/1‑148 (MQ=255)
                                       aTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGAGTGCCGTCGTTTTCATCAACGAGCTCTTTGCAGCCTAAAAGCGTGCGACAGCCTTCATCAGCa                                                                                                         >  2:3441901/1‑148 (MQ=255)
                                            gcggcgGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGaaa                                                                                                    <  1:15201/148‑1 (MQ=255)
                                             cggcggCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGaaaa                                                                                                   <  2:1884858/148‑1 (MQ=255)
                                             cggcggCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGaaaa                                                                                                   <  2:708843/148‑1 (MQ=255)
                                                 ggcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAcgcg                                                                                               <  1:3186821/148‑1 (MQ=255)
                                                 ggcTGGGTTTGCCGCTCTACCACGTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAcgcg                                                                                               <  2:1160570/148‑1 (MQ=255)
                                                  gcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACgcgc                                                                                              <  2:959759/148‑1 (MQ=255)
                                                    tGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCTCAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGACTGCTTCAGCAAGAAAACGCGCtt                                                                                            <  1:2935716/148‑1 (MQ=255)
                                                            ccGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACa                                                                                    >  2:353423/1‑148 (MQ=255)
                                                               ctctACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAAt                                                                                 <  1:2765085/148‑1 (MQ=255)
                                                                tctACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTAAACGAGCTCATTTCTGCCTAAAACCGTTCGCCACCCAGCTTCAGCAACAAAAAACGCATTTTCGACaaaat                                                                                >  1:1364005/1‑146 (MQ=255)
                                                                 ctACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACACCGGGCGCCAGCCAGCATCAACAAAAAAACCCGCTTTATCCACaaaaat                                                                               >  1:3456340/1‑145 (MQ=255)
                                                                   aCCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCGTTGCTGCCTAACAGCGTGCGCCAGCCTGCGTCAGCAAGAAAACCCGCGTTAGCGACAAAatttc                                                                             >  2:1626213/1‑146 (MQ=255)
                                                                   aCCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGCCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGc                                                                             <  2:1423643/148‑1 (MQ=255)
                                                                    ccACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTGATCGAGCTAATTGGTGCCGAACAGCGTGCGGCAGCCGGCGTAAGCAAGAAAACACGCTTTATCGACaaaatatac                                                                            >  1:3459856/1‑142 (MQ=255)
                                                                      aCTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCtt                                                                          <  1:762797/148‑1 (MQ=255)
                                                                      aCTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCtt                                                                          <  1:2523248/148‑1 (MQ=255)
                                                                       cTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCttt                                                                         <  1:1182603/148‑1 (MQ=255)
                                                                       cTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCttt                                                                         <  2:2341416/148‑1 (MQ=255)
                                                                        ttCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCAGCTTCAGCCAGAAAACGCGCGTTAGCGACAAATTTGCCTTTg                                                                        >  2:63255/1‑148 (MQ=255)
                                                                           aCCTTTTTCACACAGCAACTCTTCGCCATTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTATTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCTCTTTAGCGACAAATTTGCCTTTGGCa                                                                     <  2:2907459/148‑1 (MQ=255)
                                                                           aCCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCa                                                                     <  2:3084675/148‑1 (MQ=255)
                                                                           aCCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCa                                                                     <  1:1622804/148‑1 (MQ=255)
                                                                           aCCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCa                                                                     <  2:1066618/148‑1 (MQ=255)
                                                                            ccTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCaa                                                                    <  2:2321080/148‑1 (MQ=255)
                                                                             cTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAAt                                                                   <  1:726245/148‑1 (MQ=255)
                                                                               ttttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGt                                                                 <  1:1004724/148‑1 (MQ=255)
                                                                                tttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTc                                                                <  2:2304013/148‑1 (MQ=255)
                                                                                tttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTc                                                                <  1:676418/148‑1 (MQ=255)
                                                                                tttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTc                                                                <  1:676798/148‑1 (MQ=255)
                                                                                tttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCCTTGGCAATGTc                                                                <  2:3279381/148‑1 (MQ=255)
                                                                                       caGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTTGCAATGTCCAGTTCg                                                         >  2:1953759/1‑148 (MQ=255)
                                                                                        aGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGa                                                        <  1:1101410/148‑1 (MQ=255)
                                                                                          cAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGGCTAACAGCGGGCGCCCGCCTGCGTCAGCAAGAAAACGCGCGTTAGCGGCAAATTTTCCTTTGGGAATGTCCAGTTCGAtt                                                      >  2:2685539/1‑147 (MQ=255)
                                                                                            aCTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTGATCGCGCTCGTTGGTGCCGAACAGCGGGCGCCCGCCGGCTTCAGCAACAAAAAGCGCGTTATCGACAAAATTTCCGTTGGGCATGTCCCCTTCGATAac                                                    >  1:1341583/1‑148 (MQ=255)
                                                                                               cATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTTCTGCCTAACAGCGTGCGCCAGCCGGCTTCAGCAAGAAAACGCGCTTTATCGACAAAATTGCCTTTGGCAATGTCCAGTTCGTTAacaca                                                 >  1:1777497/1‑148 (MQ=255)
                                                                                               cATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAacaca                                                 <  1:2816796/148‑1 (MQ=255)
                                                                                                       ttCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCATCGAGCTCTTTGCTGCCTAAAAGCGTGCGCCAGCCTGCGTCAGCAAGAAAACGCGCGTTAGCGGCAAATTTGCCTTTGGCCATGTCCAGGTCGATAACACACATGCGGa                                         >  2:3020448/1‑148 (MQ=255)
                                                                                                       ttCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGa                                         <  1:1238571/148‑1 (MQ=255)
                                                                                                          gCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAaca                                      <  1:2155337/148‑1 (MQ=255)
                                                                                                          gCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAaca                                      <  1:2155261/148‑1 (MQ=255)
                                                                                                                acaGGCAGTTGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACCGCGTGCGCCCGCCGGCTTCAGCAAGAAAACGCGCCGGAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCAt                                <  1:3350794/148‑1 (MQ=255)
                                                                                                                 caGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATc                               <  1:160208/148‑1 (MQ=255)
                                                                                                                 caGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCGTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATc                               <  1:410722/148‑1 (MQ=255)
                                                                                                                   ggCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCg                             <  1:1857608/148‑1 (MQ=255)
                                                                                                                      aGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGc                          <  1:3089997/148‑1 (MQ=255)
                                                                                                                           cGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAAt                     <  2:118491/148‑1 (MQ=255)
                                                                                                                             tGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTg                   <  1:2535141/148‑1 (MQ=255)
                                                                                                                                  tcgtTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACCCCGCATCCGGGCAGAATTGCATCa              >  2:1710277/1‑148 (MQ=255)
                                                                                                                                  tcgtTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAAATTTCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCa              >  2:1709010/1‑148 (MQ=255)
                                                                                                                                  tcgtTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCa              >  2:2527090/1‑148 (MQ=255)
                                                                                                                                   cgtTTTCTTCATCGAGCTCTTTTCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAAGCGCTTTAGCGACAAATTTTCCTTTGGCAAAGTCCAGTTCGATAACACACTTGCGGAACACCCCATCCGGGCAGAATTTCATCAg             >  1:342192/1‑148 (MQ=255)
                                                                                                                                    gtTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGa            <  1:1576351/148‑1 (MQ=255)
                                                                                                                                     ttttCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGAt           <  1:2334465/148‑1 (MQ=255)
                                                                                                                                           tcATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGAc     >  1:2224298/1‑148 (MQ=255)
                                                                                                                                            cATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACg    <  2:2379441/148‑1 (MQ=255)
                                                                                                                                            cATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACg    <  2:592879/148‑1 (MQ=255)
                                                                                                                                             aTCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACgg   <  2:488098/148‑1 (MQ=255)
                                                                                                                                             aTCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACgg   >  2:3003814/1‑148 (MQ=255)
                                                                                                                                             aTCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACgg   >  2:739455/1‑148 (MQ=255)
                                                                                                                                              tCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACggg  <  1:952598/148‑1 (MQ=255)
                                                                                                                                              tCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACggg  >  1:658205/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGG  >  CP009273/1839709‑1839998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAAT  >  CP009273/1839708‑1840002
                                                                                                                                                  |                                                                                                                                                    
CGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATAGAGCT                                                                                                                                                   >  SRR19973065.2026526/1‑150 (MQ=60)
    CCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTT                                                                                                                                               <  SRR19973065.1871998/150‑1 (MQ=60)
    CCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTT                                                                                                                                               <  SRR19973065.1872012/150‑1 (MQ=60)
     CCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTGTGGTCATCGCGCTCTTTG                                                                                                                                              >  SRR19973065.1180071/1‑150 (MQ=60)
       GGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCATCGAGCTCGTTGCT                                                                                                                                            >  SRR19973065.2462725/1‑150 (MQ=60)
       GGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCT                                                                                                                                            <  SRR19973065.753492/150‑1 (MQ=60)
          CATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCC                                                                                                                                         <  SRR19973065.2470733/150‑1 (MQ=60)
            TCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTA                                                                                                                                       <  SRR19973065.2796939/150‑1 (MQ=60)
                CGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTGATCGCGCTATTTTGTGCCTAACAA                                                                                                                                   >  SRR19973065.1720062/1‑150 (MQ=60)
                CGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGCGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCATCGCGCTCTTTGGTGCCTAACAC                                                                                                                                   >  SRR19973065.3007863/1‑150 (MQ=60)
                  AAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTAATCGAGCTCTTTGCTGCCGAACAGCG                                                                                                                                 >  SRR19973065.3494516/1‑150 (MQ=60)
                    AGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCATCGAGCTCTTTGCTGCCTAACAGCGTG                                                                                                                               >  SRR19973065.2356031/1‑150 (MQ=60)
                       AGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTGATCGAGCTCTTTGCTGGCGAACAGCGTGCGC                                                                                                                            >  SRR19973065.1078168/1‑150 (MQ=60)
                       AGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTTCTGCCGAAAAGCGTGCGC                                                                                                                            >  SRR19973065.2752849/1‑150 (MQ=60)
                             GCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCT                                                                                                                      <  SRR19973065.3449902/150‑1 (MQ=60)
                              CATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTAATCGAGCTCTTTGGTGCCTAACAGCGTGCGCCAGCCTG                                                                                                                     >  SRR19973065.2548245/1‑150 (MQ=60)
                              CATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGGTGTTCATCGAGGTCTTTGCTGCCTAACAGCGTGCGCCAGCCTG                                                                                                                     >  SRR19973065.2548269/1‑150 (MQ=60)
                               ATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCATAGGGCTGGTTGCTGCCGAAAAGCGGGCGCCCGCCTGC                                                                                                                    >  SRR19973065.2005600/1‑150 (MQ=60)
                                  GGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTC                                                                                                                 <  SRR19973065.1855337/150‑1 (MQ=60)
                                  GGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTC                                                                                                                 <  SRR19973065.1855278/150‑1 (MQ=60)
                                    TAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCATCGAGCTCTTTGGTGCCGAACAGCGTGCGCCAGCCTGCTTCAG                                                                                                               >  SRR19973065.1904576/1‑150 (MQ=60)
                                      AAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCA                                                                                                             <  SRR19973065.286450/150‑1 (MQ=60)
                                        ATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAG                                                                                                           <  SRR19973065.1064638/150‑1 (MQ=60)
                                          GGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAA                                                                                                         <  SRR19973065.2581604/150‑1 (MQ=60)
                                             GCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAC                                                                                                      <  SRR19973065.15427/150‑1 (MQ=60)
                                                GCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCG                                                                                                   <  SRR19973065.1251018/150‑1 (MQ=60)
                                                  GGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTAATAGATCTCTTTTCTTCCAAACAGCCTGCGCCAGCCAGCTTCATCAAGAAAAaacccg                                                                                                 >  SRR19973065.1357964/1‑144 (MQ=60)
                                                  GGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCT                                                                                                 <  SRR19973065.3206055/150‑1 (MQ=60)
                                                  GGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCGTCATCGAGCTCTTTGCTGCCAAACAGCGTGCGCCCCCCAGCTTCACCAAGAAAACGCGCT                                                                                                 >  SRR19973065.3212403/1‑150 (MQ=60)
                                                  GGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTAATCGAGCTCTTTTCTGCCaaacaccctgcgccccccaccttcagcaacaaaacgcgcg                                                                                                 >  SRR19973065.3405107/1‑110 (MQ=60)
                                                     TGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCTCAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGACTGCTTCAGCAAGAAAACGCGCTTTA                                                                                              <  SRR19973065.2953519/150‑1 (MQ=60)
                                                                CTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCATAGAGCTCTTTTGTGCCTAACAGCGGGCGCCAGCCAGCGTCAGCACGAAAAaccgcgttatcgacaaaatt                                                                                   >  SRR19973065.2767371/1‑130 (MQ=60)
                                                                CTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTT                                                                                   <  SRR19973065.2782271/150‑1 (MQ=60)
                                                                CTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACACCGGGCGCCAGCCagcatcaacaaaaaaacccgctttatccacaaaaat                                                                                   >  SRR19973065.3477403/1‑114 (MQ=60)
                                                                CTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTAAACGAGCTCTTTTCTGCCTAAAACCCTGCGCCAGCCAGCTTCAGCAAGAAAACcccctttttcgacaaaaat                                                                                   >  SRR19973065.2767770/1‑131 (MQ=60)
                                                                   TACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCC                                                                                <  SRR19973065.1545681/150‑1 (MQ=60)
                                                                   TACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTGATCGAGCTAATTGGTGCCGAACAGCGTGCGGCAGCCGGCGTAAGCAAGAAAACACGCTTTATCGACAAAatatac                                                                                >  SRR19973065.3480934/1‑144 (MQ=60)
                                                                   TACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTCATCGAGCTCTTTtggtccgaaaaccgttcgccagccagcttaagcaagaaaaagcgcgttagcgacaaaatttca                                                                                >  SRR19973065.3480745/1‑87 (MQ=60)
                                                                      CACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTT                                                                             <  SRR19973065.253720/150‑1 (MQ=60)
                                                                       ACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTG                                                                            <  SRR19973065.2539089/150‑1 (MQ=60)
                                                                       ACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTG                                                                            <  SRR19973065.767874/150‑1 (MQ=60)
                                                                       ACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTG                                                                            <  SRR19973065.2338771/150‑1 (MQ=60)
                                                                        CTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGG                                                                           <  SRR19973065.1189754/150‑1 (MQ=60)
                                                                        CTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGG                                                                           <  SRR19973065.1190093/150‑1 (MQ=60)
                                                                        CTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGG                                                                           <  SRR19973065.1630979/150‑1 (MQ=60)
                                                                            ACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAAT                                                                       <  SRR19973065.1633682/150‑1 (MQ=60)
                                                                              CTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGT                                                                     <  SRR19973065.731111/150‑1 (MQ=60)
                                                                               TTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTC                                                                    <  SRR19973065.2339873/150‑1 (MQ=60)
                                                                               TTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTC                                                                    <  SRR19973065.3302872/150‑1 (MQ=60)
                                                                                TTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCC                                                                   <  SRR19973065.1011394/150‑1 (MQ=60)
                                                                                 TTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCA                                                                  <  SRR19973065.681309/150‑1 (MQ=60)
                                                                                 TTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCA                                                                  <  SRR19973065.680925/150‑1 (MQ=60)
                                                                                 TTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCA                                                                  <  SRR19973065.681306/150‑1 (MQ=60)
                                                                                 TTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTGTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCA                                                                  <  SRR19973065.681048/150‑1 (MQ=60)
                                                                                   TCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTAATAGAGCTCGTTGCTGCCGAACAGCGTGCGCCAGCCAGCTTCAGCAAGAAAAAGCGCGTTAGCGACAAAatttccattgggaatgtcccgg                                                                >  SRR19973065.1098659/1‑128 (MQ=60)
                                                                                     ACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTC                                                              <  SRR19973065.1601012/150‑1 (MQ=60)
                                                                                     ACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTC                                                              <  SRR19973065.2885800/150‑1 (MQ=60)
                                                                                         AGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATA                                                          <  SRR19973065.1108185/150‑1 (MQ=60)
                                                                                         AGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATA                                                          <  SRR19973065.1108647/150‑1 (MQ=60)
                                                                                           CAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTGATCGCGCTCGTTGGTGCCGAACAGCGGGCGCCCGCCGGCTTCAGCAACAAAAAGCGCGTTATCGACAAAatttccgttgggcatgtccccttcgataac                                                        >  SRR19973065.1350461/1‑120 (MQ=60)
                                                                                             ACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACAC                                                      <  SRR19973065.664419/150‑1 (MQ=60)
                                                                                              CTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCAACGAGCTCGTTTCTGCCGAAAAGCGTGCGCCAGCCAGCGTCAGCAAGAAAACGCGCGTTATCGACAAATTTTCCTTTGGGAATGTGCAGGTCGGGAACACA                                                     >  SRR19973065.3483232/1‑150 (MQ=60)
                                                                                              CTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACA                                                     <  SRR19973065.2045407/150‑1 (MQ=60)
                                                                                              CTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTTCTGCCTAACAGCGTGCGCCAGCCGGCTTCAGCAAGAAAACGCGCTTTATCGACAAAATTGCCTTTGGCAATGTCCAGTTCGTTAACACA                                                     >  SRR19973065.1789114/1‑150 (MQ=60)
                                                                                               TCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGAGCTCTTTGgggccgaacaccctgcgcccccccgcgtcagccagaaaaagcgccttatcgcccaaattgacgttgggaatgtccacgtcgcgaacaaac                                                    >  SRR19973065.2676737/1‑60 (MQ=60)
                                                                                                CATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACT                                                   <  SRR19973065.2834202/150‑1 (MQ=60)
                                                                                                 ATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTGATCGCGCTCTTTTGTGCCGAACAGCGTGCGCCcccctgcgtcagccagaaaaaccgcggtatcgacaaaaattgctttgggcatgtgcagttcgataacacacat                                                  >  SRR19973065.2477649/1‑77 (MQ=60)
                                                                                                  TCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTG                                                 <  SRR19973065.182639/150‑1 (MQ=60)
                                                                                                        TTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAAC                                           <  SRR19973065.1246700/150‑1 (MQ=60)
                                                                                                           GCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACC                                        <  SRR19973065.415783/150‑1 (MQ=60)
                                                                                                           GCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACC                                        <  SRR19973065.2169123/150‑1 (MQ=60)
                                                                                                           GCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACC                                        <  SRR19973065.2169199/150‑1 (MQ=60)
                                                                                                           GCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACC                                        <  SRR19973065.2169194/150‑1 (MQ=60)
                                                                                                           GCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACC                                        <  SRR19973065.1454168/150‑1 (MQ=60)
                                                                                                              ACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCA                                     <  SRR19973065.2843944/150‑1 (MQ=60)
                                                                                                                 ACAGGCAGTTGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACCGCGTGCGCCCGCCGGCTTCAGCAAGAAAACGCGCCGGAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCC                                  <  SRR19973065.3371269/150‑1 (MQ=60)
                                                                                                                  CAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCG                                 <  SRR19973065.161528/150‑1 (MQ=60)
                                                                                                                  CAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCGTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCG                                 <  SRR19973065.413598/150‑1 (MQ=60)
                                                                                                                    GGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGG                               <  SRR19973065.1869828/150‑1 (MQ=60)
                                                                                                                    GGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGG                               <  SRR19973065.1869911/150‑1 (MQ=60)
                                                                                                                       AGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAG                            <  SRR19973065.3108861/150‑1 (MQ=60)
                                                                                                                       AGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAG                            <  SRR19973065.3108796/150‑1 (MQ=60)
                                                                                                                       AGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAG                            <  SRR19973065.3228029/150‑1 (MQ=60)
                                                                                                                       AGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTGTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAG                            <  SRR19973065.3108947/150‑1 (MQ=60)
                                                                                                                              TGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCA                     <  SRR19973065.2551046/150‑1 (MQ=60)
                                                                                                                              TGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCA                     <  SRR19973065.2550432/150‑1 (MQ=60)
                                                                                                                                  GTCGTTTTCTTCATCGAGCTCTTTTCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAAGCGCTTTAGCGACAAATTTTCCTTTGGCAAAGTCCAGTTCGATAACACACTTGCGGAACACCCCATCCGGGCAGAATTTCATCAG                 >  SRR19973065.344583/1‑150 (MQ=60)
                                                                                                                                     GTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATA              <  SRR19973065.1586852/150‑1 (MQ=60)
                                                                                                                                      TTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATAC             <  SRR19973065.2349048/150‑1 (MQ=60)
                                                                                                                                      TTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATAC             <  SRR19973065.1191141/150‑1 (MQ=60)
                                                                                                                                          CTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGAC         >  SRR19973065.2238479/1‑150 (MQ=60)
                                                                                                                                           TTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACG        >  SRR19973065.2995031/1‑150 (MQ=60)
                                                                                                                                             CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGG      >  SRR19973065.662555/1‑150 (MQ=60)
                                                                                                                                              ATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGC     >  SRR19973065.591698/1‑150 (MQ=60)
                                                                                                                                               TCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCA    <  SRR19973065.2179048/150‑1 (MQ=60)
                                                                                                                                               TCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCA    <  SRR19973065.958893/150‑1 (MQ=60)
                                                                                                                                                 GAGCTCTTGGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAAT  <  SRR19973065.3291440/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
CGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAAT  >  CP009273/1839708‑1840002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑