Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,860,183 G→T 100% R183S (CGC→AGC)  mipA ← scaffolding protein for murein synthesizing machinery

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,860,1830GT99.5% 687.3 / ‑4.3 216R183S (CGC→AGC) mipAscaffolding protein for murein synthesizing machinery
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (88/127);  total (89/127)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 218 total aligned reads displayed.

TCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTT  >  CP009273/1860039‑1860327
                                                                                                                                                |                                                                                                                                                
   ggCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGa                                                                                                                                            <  2:938518/148‑1 (MQ=255)
   ggCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGa                                                                                                                                            >  2:1660150/1‑148 (MQ=255)
       gTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGActct                                                                                                                                        <  2:292432/148‑1 (MQ=255)
       gTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGActct                                                                                                                                        <  2:2438915/148‑1 (MQ=255)
       gTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGActct                                                                                                                                        <  2:1089165/148‑1 (MQ=255)
        tCAGTAAGTTCATCAGACAGACGGGTGTAGCGTGCGGGACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCtt                                                                                                                                       <  2:1865193/148‑1 (MQ=255)
         cAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCttt                                                                                                                                      >  1:1181661/1‑148 (MQ=255)
          aGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTACAGCCACGCAGACCGCTGCTAGCGGACTCTTTg                                                                                                                                     <  1:2843627/148‑1 (MQ=255)
           gTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTgc                                                                                                                                    >  2:537449/1‑148 (MQ=255)
              aCTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGcgcg                                                                                                                                 >  1:99995/1‑148 (MQ=255)
               cTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGa                                                                                                                                <  2:2390847/148‑1 (MQ=255)
               cTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGa                                                                                                                                >  2:3353385/1‑148 (MQ=255)
                 tcatcaGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATa                                                                                                                              <  2:431598/148‑1 (MQ=255)
                   atcaGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACg                                                                                                                            >  2:2500431/1‑148 (MQ=255)
                    tcaGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGc                                                                                                                           >  1:862779/1‑148 (MQ=255)
                       gacagacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCat                                                                                                                        <  2:2888956/148‑1 (MQ=255)
                          agacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCataat                                                                                                                     <  1:1762429/148‑1 (MQ=255)
                          agacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCataat                                                                                                                     <  1:1824693/148‑1 (MQ=255)
                          agacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCataat                                                                                                                     <  2:2603837/148‑1 (MQ=255)
                           gacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCataata                                                                                                                    <  2:2804483/148‑1 (MQ=255)
                           gacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCataata                                                                                                                    >  2:2758943/1‑148 (MQ=255)
                            acGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAg                                                                                                                   <  1:3070703/148‑1 (MQ=255)
                             cGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGt                                                                                                                  <  1:953696/148‑1 (MQ=255)
                               ggTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTAt                                                                                                                <  1:516726/148‑1 (MQ=255)
                               ggTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTAt                                                                                                                <  1:1930119/148‑1 (MQ=255)
                               ggTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTAt                                                                                                                <  2:2275389/148‑1 (MQ=255)
                                        tGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTgg                                                                                                       <  2:535087/148‑1 (MQ=255)
                                         gCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGt                                                                                                      <  2:524514/148‑1 (MQ=255)
                                          cGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGtt                                                                                                     <  1:1462571/148‑1 (MQ=255)
                                           ggTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGttt                                                                                                    <  2:3081516/148‑1 (MQ=255)
                                           ggTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGttt                                                                                                    <  2:17380/148‑1 (MQ=255)
                                           ggTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGttt                                                                                                    <  1:2763772/148‑1 (MQ=255)
                                           ggTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGttt                                                                                                    <  2:1795177/148‑1 (MQ=255)
                                             tACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTc                                                                                                  >  1:3161084/1‑148 (MQ=255)
                                              aCCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCg                                                                                                 <  1:2728497/148‑1 (MQ=255)
                                               ccGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCTCGATACGCCATAATAGTATTCGTTCTGGTTTTCGc                                                                                                <  1:1253300/148‑1 (MQ=255)
                                                 gTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTg                                                                                              <  2:377444/148‑1 (MQ=255)
                                                     acacTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCCTAAAAGTATTCGTTCTGGTTTTCGCTGTTcc                                                                                          >  1:3487976/1‑148 (MQ=255)
                                                     acacTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCAGAATAGTATTCGTTCTGGTTTTCGCTGTTcc                                                                                          >  2:3274736/1‑148 (MQ=255)
                                                          ccAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGc                                                                                     <  2:895288/148‑1 (MQ=255)
                                                           cAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGca                                                                                    <  1:3013322/148‑1 (MQ=255)
                                                            aGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGcac                                                                                   <  1:2153646/148‑1 (MQ=255)
                                                              tCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCacac                                                                                 <  1:2320723/148‑1 (MQ=255)
                                                                gCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCa                                                                               <  1:2895469/148‑1 (MQ=255)
                                                                 ccGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCaa                                                                              <  1:1671353/148‑1 (MQ=255)
                                                                  cGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAAt                                                                             <  1:2359720/148‑1 (MQ=255)
                                                                   gAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATa                                                                            <  1:1891296/148‑1 (MQ=255)
                                                                     ggAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATAcc                                                                          <  1:841053/148‑1 (MQ=255)
                                                                     ggAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATAcc                                                                          >  1:2782683/1‑148 (MQ=255)
                                                                     ggAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATAcc                                                                          >  1:1454388/1‑148 (MQ=255)
                                                                     ggAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATAcc                                                                          >  1:3480761/1‑148 (MQ=255)
                                                                      gAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATAccc                                                                         <  2:3172973/148‑1 (MQ=255)
                                                                           tGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGt                                                                    <  1:1600647/148‑1 (MQ=255)
                                                                               gCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAAAACCCGGAGTCACg                                                                >  1:177044/1‑148 (MQ=255)
                                                                                cTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACgg                                                               >  2:1058098/1‑148 (MQ=255)
                                                                                 tGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGt                                                              <  2:1665780/148‑1 (MQ=255)
                                                                                   gcgcTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCa                                                            <  1:174913/148‑1 (MQ=255)
                                                                                        cAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCa                                                       >  1:2628659/1‑148 (MQ=255)
                                                                                        cAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCa                                                       >  2:2991380/1‑148 (MQ=255)
                                                                                        cAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTATTTGCGCGATACGCCATAATATTATACGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCATGCCa                                                       >  2:1531281/1‑148 (MQ=255)
                                                                                           cTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCg                                                    >  1:1933741/1‑148 (MQ=255)
                                                                                           cTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCg                                                    <  1:2665162/148‑1 (MQ=255)
                                                                                           cTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCg                                                    >  1:2348146/1‑148 (MQ=255)
                                                                                           cTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCg                                                    <  1:2453459/148‑1 (MQ=255)
                                                                                             ccAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGtt                                                  <  2:2657381/148‑1 (MQ=255)
                                                                                             ccAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGtt                                                  >  2:1389759/1‑148 (MQ=255)
                                                                                              cAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTg                                                 <  2:2146251/148‑1 (MQ=255)
                                                                                                ggTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGgt                                               >  1:2640134/1‑148 (MQ=255)
                                                                                                ggTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGgt                                               >  2:1329911/1‑148 (MQ=255)
                                                                                                 gTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGgtg                                              >  2:1150979/1‑148 (MQ=255)
                                                                                                  tAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGgtgt                                             >  2:2169223/1‑148 (MQ=255)
                                                                                                    aGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTaa                                           >  2:684599/1‑148 (MQ=255)
                                                                                                    aGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTaa                                           <  2:1084824/148‑1 (MQ=255)
                                                                                                      ggCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACg                                         <  1:481738/148‑1 (MQ=255)
                                                                                                      ggCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACg                                         <  1:749662/148‑1 (MQ=255)
                                                                                                       gCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGa                                        <  2:30873/148‑1 (MQ=255)
                                                                                                         tCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATa                                      <  2:1204582/148‑1 (MQ=255)
                                                                                                          ccAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCATTGCGCGATACGCCATAATAGTATTAGTTCTGGTTTTCGCTGTTACACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATAc                                     >  1:1651562/1‑148 (MQ=255)
                                                                                                           cAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACa                                    >  2:3083062/1‑148 (MQ=255)
                                                                                                            aGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACaa                                   >  2:3057458/1‑148 (MQ=255)
                                                                                                              cTGTCGTTCGGGTTATATCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAAcc                                 >  1:828276/1‑148 (MQ=255)
                                                                                                              cTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCAGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAAcc                                 >  1:2586360/1‑148 (MQ=255)
                                                                                                               tGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCa                                <  2:2535520/148‑1 (MQ=255)
                                                                                                                gTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAg                               >  2:177093/1‑148 (MQ=255)
                                                                                                                     tCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCat                          >  1:1895347/1‑148 (MQ=255)
                                                                                                                       gggTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCatat                        <  2:3049150/148‑1 (MQ=255)
                                                                                                                         gTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCTTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATcc                      <  2:1460756/148‑1 (MQ=255)
                                                                                                                         gTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATcc                      >  1:297992/1‑148 (MQ=255)
                                                                                                                          ttATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATccc                     <  2:2841456/148‑1 (MQ=255)
                                                                                                                          ttATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAAGAGGATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATccc                     <  2:471743/148‑1 (MQ=255)
                                                                                                                                gcACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGa               <  2:936089/147‑1 (MQ=255)
                                                                                                                                  aCGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATg             >  1:1000168/1‑148 (MQ=255)
                                                                                                                                  aCGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATg             <  2:862779/148‑1 (MQ=255)
                                                                                                                                    gCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGcc           >  2:631600/1‑148 (MQ=255)
                                                                                                                                     cAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTAGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCg          <  1:135805/148‑1 (MQ=255)
                                                                                                                                       gACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGtt        >  2:3456505/1‑148 (MQ=255)
                                                                                                                                        aCCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTg       <  1:210667/148‑1 (MQ=255)
                                                                                                                                           gctgctAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTg    <  2:2852494/148‑1 (MQ=255)
                                                                                                                                            cTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGt   >  2:868052/1‑148 (MQ=255)
                                                                                                                                            cTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTACACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGt   >  1:3129606/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTT  >  CP009273/1860039‑1860327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTATCC  >  CP009273/1860034‑1860331
                                                                                                                                                     |                                                                                                                                                    
CACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCT                                                                                                                                                      <  SRR19973065.2787884/150‑1 (MQ=60)
CACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCT                                                                                                                                                      >  SRR19973065.2601952/1‑150 (MQ=60)
CACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCT                                                                                                                                                      <  SRR19973065.2588489/150‑1 (MQ=60)
CACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCT                                                                                                                                                      <  SRR19973065.1972054/150‑1 (MQ=60)
  CCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAG                                                                                                                                                    <  SRR19973065.2572952/150‑1 (MQ=60)
        GGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACT                                                                                                                                              <  SRR19973065.2140726/150‑1 (MQ=60)
          CTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCT                                                                                                                                            <  SRR19973065.2608335/150‑1 (MQ=60)
           TGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTT                                                                                                                                           >  SRR19973065.2513338/1‑150 (MQ=60)
            GTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTT                                                                                                                                          >  SRR19973065.1189146/1‑150 (MQ=60)
               AGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTACAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCG                                                                                                                                       <  SRR19973065.2861130/150‑1 (MQ=60)
                 TAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCG                                                                                                                                     >  SRR19973065.100913/1‑150 (MQ=60)
                       CATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGC                                                                                                                               >  SRR19973065.868331/1‑150 (MQ=60)
                       CATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTGCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGC                                                                                                                               >  SRR19973065.2747659/1‑150 (MQ=60)
                        ATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCC                                                                                                                              >  SRR19973065.1827639/1‑150 (MQ=60)
                               AGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAG                                                                                                                       <  SRR19973065.3519952/150‑1 (MQ=60)
                               AGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAG                                                                                                                       <  SRR19973065.1836706/150‑1 (MQ=60)
                               AGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAG                                                                                                                       <  SRR19973065.1773994/150‑1 (MQ=60)
                                 ACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTA                                                                                                                     <  SRR19973065.3089471/150‑1 (MQ=60)
                                 ACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTA                                                                                                                     <  SRR19973065.1334133/150‑1 (MQ=60)
                                  CGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTAT                                                                                                                    <  SRR19973065.960000/150‑1 (MQ=60)
                                   GGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATT                                                                                                                   <  SRR19973065.1163237/150‑1 (MQ=60)
                                   GGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATT                                                                                                                   <  SRR19973065.1904918/150‑1 (MQ=60)
                                    GGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTC                                                                                                                  <  SRR19973065.1942762/150‑1 (MQ=60)
                                    GGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTC                                                                                                                  <  SRR19973065.1724079/150‑1 (MQ=60)
                                    GGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTC                                                                                                                  <  SRR19973065.1942782/150‑1 (MQ=60)
                                    GGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTC                                                                                                                  <  SRR19973065.520144/150‑1 (MQ=60)
                                    GGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTC                                                                                                                  <  SRR19973065.1367573/150‑1 (MQ=60)
                                       GTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGGT                                                                                                               >  SRR19973065.1816642/1‑150 (MQ=60)
                                          GCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTG                                                                                                            <  SRR19973065.1910433/150‑1 (MQ=60)
                                             TGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTT                                                                                                         <  SRR19973065.2723909/150‑1 (MQ=60)
                                               CGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTT                                                                                                       <  SRR19973065.3484627/150‑1 (MQ=60)
                                               CGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTT                                                                                                       <  SRR19973065.1471969/150‑1 (MQ=60)
                                                GGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTC                                                                                                      >  SRR19973065.3067803/1‑150 (MQ=60)
                                                GGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTC                                                                                                      >  SRR19973065.3067570/1‑150 (MQ=60)
                                                GGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTC                                                                                                      >  SRR19973065.3180235/1‑150 (MQ=60)
                                                GGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTC                                                                                                      <  SRR19973065.2780948/150‑1 (MQ=60)
                                                GGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTC                                                                                                      <  SRR19973065.2780954/150‑1 (MQ=60)
                                                 GTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCG                                                                                                     <  SRR19973065.2526518/150‑1 (MQ=60)
                                                   ACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCT                                                                                                   <  SRR19973065.1781546/150‑1 (MQ=60)
                                                   ACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCT                                                                                                   <  SRR19973065.2745468/150‑1 (MQ=60)
                                                    CCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCTCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTG                                                                                                  <  SRR19973065.1261554/150‑1 (MQ=60)
                                                        AAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCATACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCC                                                                                              >  SRR19973065.3509051/1‑150 (MQ=60)
                                                        AAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCCTAAAAGTATTCGTTCTGGTTTTCGCTGTTCC                                                                                              >  SRR19973065.3509186/1‑150 (MQ=60)
                                                         AACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCA                                                                                             <  SRR19973065.959522/150‑1 (MQ=60)
                                                           CACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCCTAATAGTATTCGTTCTGGTTTTAGCTGTTCCACT                                                                                           >  SRR19973065.1238361/1‑150 (MQ=60)
                                                           CACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACT                                                                                           <  SRR19973065.2015429/150‑1 (MQ=60)
                                                                CAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATATCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACA                                                                                      >  SRR19973065.3022124/1‑150 (MQ=60)
                                                                CAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACA                                                                                      <  SRR19973065.3031776/150‑1 (MQ=60)
                                                                 AGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACAC                                                                                     <  SRR19973065.2167501/150‑1 (MQ=60)
                                                                 AGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACAC                                                                                     <  SRR19973065.2167497/150‑1 (MQ=60)
                                                                   TCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCA                                                                                   <  SRR19973065.2335267/150‑1 (MQ=60)
                                                                     GCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAAT                                                                                 <  SRR19973065.1671269/150‑1 (MQ=60)
                                                                     GCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAAT                                                                                 <  SRR19973065.2913147/150‑1 (MQ=60)
                                                                      CCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATA                                                                                <  SRR19973065.1682532/150‑1 (MQ=60)
                                                                       CGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATAC                                                                               <  SRR19973065.2374391/150‑1 (MQ=60)
                                                                       CGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATAC                                                                               <  SRR19973065.2372603/150‑1 (MQ=60)
                                                                        GAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACC                                                                              <  SRR19973065.2899639/150‑1 (MQ=60)
                                                                        GAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACC                                                                              >  SRR19973065.2799948/1‑150 (MQ=60)
                                                                        GAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACC                                                                              >  SRR19973065.1463758/1‑150 (MQ=60)
                                                                        GAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACC                                                                              >  SRR19973065.1463809/1‑150 (MQ=60)
                                                                        GAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACC                                                                              <  SRR19973065.1903730/150‑1 (MQ=60)
                                                                        GAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACC                                                                              >  SRR19973065.3501943/1‑150 (MQ=60)
                                                                          GGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCG                                                                            <  SRR19973065.846527/150‑1 (MQ=60)
                                                                          GGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCG                                                                            <  SRR19973065.846321/150‑1 (MQ=60)
                                                                          GGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCG                                                                            <  SRR19973065.743539/150‑1 (MQ=60)
                                                                             AGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATATCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATAACCGGAG                                                                         >  SRR19973065.92749/1‑150 (MQ=60)
                                                                                TGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCA                                                                      <  SRR19973065.1611343/150‑1 (MQ=60)
                                                                                  TAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAAAACCCGGAGTCACG                                                                    >  SRR19973065.178446/1‑150 (MQ=60)
                                                                                        GCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGG                                                              <  SRR19973065.176305/150‑1 (MQ=60)
                                                                                        GCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGG                                                              <  SRR19973065.177612/150‑1 (MQ=60)
                                                                                           CTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCA                                                           >  SRR19973065.2644918/1‑150 (MQ=60)
                                                                                           CTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCA                                                           >  SRR19973065.2644951/1‑150 (MQ=60)
                                                                                             CAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACC                                                         <  SRR19973065.237824/150‑1 (MQ=60)
                                                                                              AGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCG                                                        >  SRR19973065.2362780/1‑150 (MQ=60)
                                                                                              AGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCG                                                        >  SRR19973065.1946397/1‑150 (MQ=60)
                                                                                                CTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTT                                                      <  SRR19973065.2681656/150‑1 (MQ=60)
                                                                                                CTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTT                                                      <  SRR19973065.2468920/150‑1 (MQ=60)
                                                                                                   CAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGT                                                   >  SRR19973065.2656434/1‑150 (MQ=60)
                                                                                                   CAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAAAACCCGGAGTCACGGTCAGGCCACCGTTGGT                                                   >  SRR19973065.2656462/1‑150 (MQ=60)
                                                                                                      GTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTA                                                >  SRR19973065.2833559/1‑150 (MQ=60)
                                                                                                      GTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTA                                                >  SRR19973065.2339291/1‑150 (MQ=60)
                                                                                                       TAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAA                                               >  SRR19973065.3512539/1‑150 (MQ=60)
                                                                                                           GGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGAT                                           <  SRR19973065.754669/150‑1 (MQ=60)
                                                                                                           GGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGAT                                           <  SRR19973065.485015/150‑1 (MQ=60)
                                                                                                           GGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGAT                                           <  SRR19973065.3143287/150‑1 (MQ=60)
                                                                                                             CTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCATTGCGCGATACGCCATAATAGTATTAGTTCTGGTTTTCGCTGTTACACTGCACACCAATAACCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATAC                                         >  SRR19973065.1662639/1‑150 (MQ=60)
                                                                                                                CAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTCCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAAC                                      <  SRR19973065.1593230/150‑1 (MQ=60)
                                                                                                                 AGCTGTCGTTCGGGTTATATCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACC                                     >  SRR19973065.833695/1‑150 (MQ=60)
                                                                                                                 AGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACC                                     <  SRR19973065.1654924/150‑1 (MQ=60)
                                                                                                                 AGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCAGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACC                                     >  SRR19973065.2602468/1‑150 (MQ=60)
                                                                                                                   CTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAG                                   >  SRR19973065.969571/1‑150 (MQ=60)
                                                                                                                        GTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCAT                              >  SRR19973065.1907801/1‑150 (MQ=60)
                                                                                                                            GGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCC                          >  SRR19973065.299915/1‑150 (MQ=60)
                                                                                                                             GGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCC                         <  SRR19973065.3158497/150‑1 (MQ=60)
                                                                                                                              GTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCA                        >  SRR19973065.1894808/1‑150 (MQ=60)
                                                                                                                                  TAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACG                    >  SRR19973065.2087299/1‑150 (MQ=60)
                                                                                                                                   AGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGA                   >  SRR19973065.863105/1‑150 (MQ=60)
                                                                                                                                     CCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATG                 >  SRR19973065.1006814/1‑150 (MQ=60)
                                                                                                                                          CAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTAGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTT            <  SRR19973065.136969/150‑1 (MQ=60)
                                                                                                                                             ACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCT         <  SRR19973065.212265/150‑1 (MQ=60)
                                                                                                                                             ACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCT         <  SRR19973065.152837/150‑1 (MQ=60)
                                                                                                                                              CCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTG        <  SRR19973065.804279/150‑1 (MQ=60)
                                                                                                                                               CGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTACACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGT       >  SRR19973065.3148652/1‑150 (MQ=60)
                                                                                                                                                GCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTT      >  SRR19973065.2969955/1‑150 (MQ=60)
                                                                                                                                                 CTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTA     >  SRR19973065.3321195/1‑150 (MQ=60)
                                                                                                                                                 CTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTA     <  SRR19973065.493645/150‑1 (MQ=60)
                                                                                                                                                 CTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTA     <  SRR19973065.2745812/150‑1 (MQ=60)
                                                                                                                                                    CTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTATCC  >  SRR19973065.1146245/1‑150 (MQ=60)
                                                                                                                                                    CTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTATCC  <  SRR19973065.2538622/150‑1 (MQ=60)
                                                                                                                                                    CTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTATCC  >  SRR19973065.1146011/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTATCC  >  CP009273/1860034‑1860331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑