Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 463,179 C→A 91.0% I104I (ATC→ATA)  cof → thiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273463,1790CA91.0% 757.9 / 65.1 276I104I (ATC→ATA) cofthiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase
Reads supporting (aligned to +/- strand):  ref base C (12/13);  new base A (123/128);  total (135/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 281 total aligned reads displayed.

CGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGA  >  CP009273/463031‑463325
                                                                                                                                                    |                                                                                                                                                  
cgcTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCTGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATc                                                                                                                                                    <  1:4674771/149‑1 (MQ=255)
   tATCGCTGGATGCGTATTTGAGTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTc                                                                                                                                                 <  2:4633401/149‑1 (MQ=255)
     tCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCaa                                                                                                                                               >  1:1228297/1‑149 (MQ=255)
       gCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATg                                                                                                                                             >  1:2818868/1‑149 (MQ=255)
        cTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAACga                                                                                                                                            <  1:4377382/149‑1 (MQ=255)
         tGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATgac                                                                                                                                           <  1:185452/149‑1 (MQ=255)
         tGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATgac                                                                                                                                           >  1:1707829/1‑149 (MQ=255)
            aTGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATgacgac                                                                                                                                        <  1:3775072/149‑1 (MQ=255)
              gCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACgg                                                                                                                                      <  1:2182225/149‑1 (MQ=255)
               cGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggt                                                                                                                                     <  1:3266321/149‑1 (MQ=255)
                   tttGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCCGCATGCATATATTCAATGACGACggttggt                                                                                                                                 >  1:4612007/1‑149 (MQ=255)
                   tttGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggttggt                                                                                                                                 >  1:3228984/1‑149 (MQ=255)
                   tttGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggttggt                                                                                                                                 >  2:1593292/1‑149 (MQ=255)
                    ttGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggttggtt                                                                                                                                <  2:1013420/149‑1 (MQ=255)
                      gATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTa                                                                                                                              <  1:4272617/149‑1 (MQ=255)
                         tACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCg                                                                                                                           <  2:3088836/149‑1 (MQ=255)
                          aCCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCgg                                                                                                                          >  2:839689/1‑149 (MQ=255)
                             ggCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGaa                                                                                                                       <  2:3759805/149‑1 (MQ=255)
                              gCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGaaa                                                                                                                      >  1:3550276/1‑149 (MQ=255)
                                 aCGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAagag                                                                                                                   >  2:2009184/1‑149 (MQ=255)
                                 aCGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAagag                                                                                                                   >  1:3718302/1‑149 (MQ=255)
                                 aCGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAagag                                                                                                                   >  1:3594985/1‑149 (MQ=255)
                                 aCGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAagag                                                                                                                   >  2:615757/1‑149 (MQ=255)
                                 aCGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAagag                                                                                                                   >  1:1195345/1‑149 (MQ=255)
                                  cGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAgaga                                                                                                                  <  2:1451125/149‑1 (MQ=255)
                                  cGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAgaga                                                                                                                  <  2:946229/149‑1 (MQ=255)
                                   ggAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGAt                                                                                                                 <  2:2109021/149‑1 (MQ=255)
                                    gAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATc                                                                                                                >  1:3041479/1‑149 (MQ=255)
                                     aaCGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATcc                                                                                                               >  1:3458864/1‑149 (MQ=255)
                                       cgcgcgTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCt                                                                                                             <  1:4094282/149‑1 (MQ=255)
                                       cgcgcgTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCt                                                                                                             <  1:2618249/149‑1 (MQ=255)
                                        gcgcgTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTg                                                                                                            <  2:520940/149‑1 (MQ=255)
                                         cgcgTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGc                                                                                                           <  1:2250265/149‑1 (MQ=255)
                                            gTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGACAGAGATCCCTGCgtt                                                                                                        >  2:1884106/1‑149 (MQ=255)
                                            gTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgtt                                                                                                        >  2:1884266/1‑149 (MQ=255)
                                             tGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgttg                                                                                                       <  1:1791798/149‑1 (MQ=255)
                                              gCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgttgt                                                                                                      >  2:1312215/1‑149 (MQ=255)
                                              gCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgttgt                                                                                                      <  2:3856820/149‑1 (MQ=255)
                                               cATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgttgtt                                                                                                     <  1:2049557/149‑1 (MQ=255)
                                               cATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgttgtt                                                                                                     >  1:2390775/1‑149 (MQ=255)
                                               cATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgttgtt                                                                                                     <  1:4651218/149‑1 (MQ=255)
                                                 ttCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGc                                                                                                   >  2:2230254/1‑149 (MQ=255)
                                                    tctGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAgg                                                                                                >  1:866666/1‑149 (MQ=255)
                                                       ggAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCAt                                                                                             <  1:63576/149‑1 (MQ=255)
                                                       ggAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCAt                                                                                             <  2:3228094/149‑1 (MQ=255)
                                                           ggTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGt                                                                                         <  1:3071684/149‑1 (MQ=255)
                                                             tGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCt                                                                                       <  1:2779096/149‑1 (MQ=255)
                                                                aCTTTTACATCGTGATGATTTACCTGCGGATGTCGCTGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCtata                                                                                    <  2:1944551/149‑1 (MQ=255)
                                                                 cTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAg                                                                                   >  2:438233/1‑149 (MQ=255)
                                                                  ttttACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGc                                                                                  >  2:2939991/1‑149 (MQ=255)
                                                                   tttACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCg                                                                                 >  1:1673806/1‑149 (MQ=255)
                                                                    ttACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGGATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCgg                                                                                <  2:2487653/149‑1 (MQ=255)
                                                                       cATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGttt                                                                             <  2:4242040/149‑1 (MQ=255)
                                                                        aTCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGtttt                                                                            >  1:4020897/1‑149 (MQ=255)
                                                                        aTCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGtttt                                                                            <  1:3888594/149‑1 (MQ=255)
                                                                        aTCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGtttt                                                                            <  2:581177/149‑1 (MQ=255)
                                                                           gTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGt                                                                         <  2:4094283/149‑1 (MQ=255)
                                                                           gTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGt                                                                         >  1:3558700/1‑149 (MQ=255)
                                                                           gTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGt                                                                         >  1:870949/1‑149 (MQ=255)
                                                                            tgatgaTTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGtt                                                                        <  2:3594985/149‑1 (MQ=255)
                                                                            tgatgaTTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGtt                                                                        <  2:3158416/149‑1 (MQ=255)
                                                                                gatTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCa                                                                    <  2:2138219/149‑1 (MQ=255)
                                                                                gatTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCa                                                                    <  1:2540230/149‑1 (MQ=255)
                                                                                 atTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAg                                                                   >  1:2414167/1‑149 (MQ=255)
                                                                                  tttACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGa                                                                  <  1:2727092/149‑1 (MQ=255)
                                                                                    tACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATa                                                                <  1:1720058/149‑1 (MQ=255)
                                                                                    tACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATa                                                                >  2:4544585/1‑149 (MQ=255)
                                                                                     aCCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATaa                                                               <  2:4031028/149‑1 (MQ=255)
                                                                                       cTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATc                                                             <  2:1498108/149‑1 (MQ=255)
                                                                                        tGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCg                                                            >  2:3690563/1‑149 (MQ=255)
                                                                                         gCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGa                                                           <  2:4700221/149‑1 (MQ=255)
                                                                                          cGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGAt                                                          <  1:2466014/149‑1 (MQ=255)
                                                                                           ggATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATg                                                         >  1:4506002/1‑149 (MQ=255)
                                                                                            gATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGt                                                        >  1:1451295/1‑149 (MQ=255)
                                                                                              tGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCa                                                      >  2:4433518/1‑149 (MQ=255)
                                                                                                 cgcgGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTAGTCTATAGCGGATTTCGTTATCAGATAATCGATGTCaaaa                                                   >  2:1176380/1‑149 (MQ=255)
                                                                                                 cgcgGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGAATATATTCAATGACGACGGTAGGTTTACCGGGAAAGAGATCCCTGCGTTGTAGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCaaaa                                                   >  2:3564273/1‑149 (MQ=255)
                                                                                                       gCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGc                                             <  2:1761183/149‑1 (MQ=255)
                                                                                                             gCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCg                                       >  2:2948830/1‑149 (MQ=255)
                                                                                                              cTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCgg                                      >  1:4064857/1‑149 (MQ=255)
                                                                                                              cTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCgg                                      >  1:4290311/1‑149 (MQ=255)
                                                                                                                 tATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAg                                   >  2:2335403/1‑149 (MQ=255)
                                                                                                                    cAGCAATGGGATACCCGAGCCAGCCTGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGt                                >  2:4250617/1‑149 (MQ=255)
                                                                                                                    cAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGt                                >  2:3509041/1‑149 (MQ=255)
                                                                                                                    cAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGt                                >  1:1746386/1‑149 (MQ=255)
                                                                                                                       cAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCAc                             <  1:3613202/149‑1 (MQ=255)
                                                                                                                          tGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCaa                          <  2:1890592/149‑1 (MQ=255)
                                                                                                                          tGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCaa                          <  1:1503654/149‑1 (MQ=255)
                                                                                                                             gATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGAt                       <  2:4061702/149‑1 (MQ=255)
                                                                                                                                 cccGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGc                   <  1:4346779/149‑1 (MQ=255)
                                                                                                                                 cccGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGc                   <  2:4718316/149‑1 (MQ=255)
                                                                                                                                 cccGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGc                   >  2:1057673/1‑149 (MQ=255)
                                                                                                                                      gCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCtg              >  2:1165927/1‑149 (MQ=255)
                                                                                                                                         cgcatgcatATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATCATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTgg           >  2:4437979/2‑149 (MQ=255)
                                                                                                                                          gcatgcatATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGc          <  2:2390775/149‑1 (MQ=255)
                                                                                                                                             tgcatATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGAt       <  2:3137143/149‑1 (MQ=255)
                                                                                                                                              gcatATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATc      >  2:27872/1‑149 (MQ=255)
                                                                                                                                               catATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCa     <  2:2448495/149‑1 (MQ=255)
                                                                                                                                               catATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCa     <  2:3010870/149‑1 (MQ=255)
                                                                                                                                                  atCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCacga  <  2:3793037/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGA  >  CP009273/463031‑463325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCAC  >  CP009273/463031‑463323
                                                                                                                                                    |                                                                                                                                                
CGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCTGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCT                                                                                                                                                 <  SRR19973071.4691410/150‑1 (MQ=60)
CGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATAT                                                                                                                                                 >  SRR19973071.1759260/1‑150 (MQ=60)
   TATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCA                                                                                                                                              <  SRR19973071.3703838/150‑1 (MQ=60)
   TATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCA                                                                                                                                              <  SRR19973071.3703998/150‑1 (MQ=60)
    ATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAA                                                                                                                                             >  SRR19973071.1232616/1‑150 (MQ=60)
     TCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAAT                                                                                                                                            <  SRR19973071.3270804/150‑1 (MQ=60)
      CGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATG                                                                                                                                           <  SRR19973071.2002695/150‑1 (MQ=60)
      CGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATG                                                                                                                                           >  SRR19973071.2828835/1‑150 (MQ=60)
        CTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGAC                                                                                                                                         >  SRR19973071.1714162/1‑150 (MQ=60)
        CTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAACGAC                                                                                                                                         <  SRR19973071.4392760/150‑1 (MQ=60)
         TGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACG                                                                                                                                        <  SRR19973071.186222/150‑1 (MQ=60)
         TGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACG                                                                                                                                        <  SRR19973071.2589118/150‑1 (MQ=60)
            ATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACG                                                                                                                                     <  SRR19973071.3788491/150‑1 (MQ=60)
             TGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGG                                                                                                                                    >  SRR19973071.1292373/1‑150 (MQ=60)
             TGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGG                                                                                                                                    >  SRR19973071.4582503/1‑150 (MQ=60)
              GCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGT                                                                                                                                   <  SRR19973071.2190300/150‑1 (MQ=60)
               CGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTT                                                                                                                                  <  SRR19973071.3277870/150‑1 (MQ=60)
                  ATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCCGCATGCATATATTCAATGACGACGGTTGGT                                                                                                                               >  SRR19973071.4628451/1‑150 (MQ=60)
                  ATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGT                                                                                                                               >  SRR19973071.3240332/1‑150 (MQ=60)
                    TTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTT                                                                                                                             <  SRR19973071.2825918/150‑1 (MQ=60)
                      GATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTAC                                                                                                                           <  SRR19973071.4287760/150‑1 (MQ=60)
                       ATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACC                                                                                                                          >  SRR19973071.331841/1‑150 (MQ=60)
                       ATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACC                                                                                                                          >  SRR19973071.1691196/1‑150 (MQ=60)
                          ACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGG                                                                                                                       <  SRR19973071.2362151/150‑1 (MQ=60)
                             GGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAA                                                                                                                    >  SRR19973071.3562665/1‑150 (MQ=60)
                                AACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGACAGAG                                                                                                                 >  SRR19973071.3607475/1‑150 (MQ=60)
                                AACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAG                                                                                                                 >  SRR19973071.1199427/1‑150 (MQ=60)
                                AACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAG                                                                                                                 >  SRR19973071.3577710/1‑150 (MQ=60)
                                AACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAG                                                                                                                 >  SRR19973071.3577754/1‑150 (MQ=60)
                                AACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAG                                                                                                                 >  SRR19973071.3731476/1‑150 (MQ=60)
                                AACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAG                                                                                                                 >  SRR19973071.3957271/1‑150 (MQ=60)
                                AACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAG                                                                                                                 >  SRR19973071.2549285/1‑150 (MQ=60)
                                   GGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATC                                                                                                              <  SRR19973071.2860851/150‑1 (MQ=60)
                                   GGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATC                                                                                                              >  SRR19973071.3052294/1‑150 (MQ=60)
                                    GAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCC                                                                                                             >  SRR19973071.3471067/1‑150 (MQ=60)
                                     AACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCC                                                                                                            >  SRR19973071.3072150/1‑150 (MQ=60)
                                       CGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTG                                                                                                          <  SRR19973071.4108828/150‑1 (MQ=60)
                                       CGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTG                                                                                                          <  SRR19973071.2627607/150‑1 (MQ=60)
                                       CGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTG                                                                                                          <  SRR19973071.2627701/150‑1 (MQ=60)
                                         CGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCG                                                                                                        <  SRR19973071.2258561/150‑1 (MQ=60)
                                            GTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGACAGAGATCCCTGCGTTG                                                                                                     >  SRR19973071.2092813/1‑150 (MQ=60)
                                            GTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTG                                                                                                     >  SRR19973071.2652821/1‑150 (MQ=60)
                                             TGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGT                                                                                                    <  SRR19973071.1798411/150‑1 (MQ=60)
                                             TGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGT                                                                                                    <  SRR19973071.1798473/150‑1 (MQ=60)
                                             TGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGT                                                                                                    <  SRR19973071.1798399/150‑1 (MQ=60)
                                             TGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGT                                                                                                    >  SRR19973071.1819095/1‑150 (MQ=60)
                                              GCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTT                                                                                                   >  SRR19973071.2399372/1‑150 (MQ=60)
                                              GCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTT                                                                                                   <  SRR19973071.3400742/150‑1 (MQ=60)
                                               CATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTG                                                                                                  >  SRR19973071.2771153/1‑150 (MQ=60)
                                               CATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTG                                                                                                  <  SRR19973071.2057076/150‑1 (MQ=60)
                                               CATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTG                                                                                                  <  SRR19973071.4667777/150‑1 (MQ=60)
                                               CATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTG                                                                                                  <  SRR19973071.4667783/150‑1 (MQ=60)
                                                ATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGATGGTTGGTTTACCGGGACAGAGATCCCTGCGTTGTTGC                                                                                                 >  SRR19973071.2227474/1‑150 (MQ=60)
                                                ATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGC                                                                                                 >  SRR19973071.2227468/1‑150 (MQ=60)
                                                   CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGG                                                                                              <  SRR19973071.3827422/150‑1 (MQ=60)
                                                   CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGG                                                                                              >  SRR19973071.869773/1‑150 (MQ=60)
                                                   CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGG                                                                                              >  SRR19973071.869767/1‑150 (MQ=60)
                                                     CTGGAAGGTGAACTTGTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCA                                                                                            <  SRR19973071.1475240/150‑1 (MQ=60)
                                                       GGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATT                                                                                          <  SRR19973071.63931/150‑1 (MQ=60)
                                                        GAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTT                                                                                         <  SRR19973071.1439695/150‑1 (MQ=60)
                                                          AGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGT                                                                                       <  SRR19973071.2027127/150‑1 (MQ=60)
                                                           GGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTC                                                                                      >  SRR19973071.4070484/1‑150 (MQ=60)
                                                           GGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTC                                                                                      <  SRR19973071.3082565/150‑1 (MQ=60)
                                                            GTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGACAGAGATCCCTGCGTTGTTGCAGGCATTTGTCT                                                                                     >  SRR19973071.3763001/1‑150 (MQ=60)
                                                            GTGAACTTTAACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCT                                                                                     >  SRR19973071.3763136/1‑150 (MQ=60)
                                                             TGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTA                                                                                    <  SRR19973071.2788868/150‑1 (MQ=60)
                                                               AACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATA                                                                                  >  SRR19973071.4497984/1‑150 (MQ=60)
                                                               AACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATA                                                                                  >  SRR19973071.4497974/1‑150 (MQ=60)
                                                               AACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATA                                                                                  >  SRR19973071.1535369/1‑150 (MQ=60)
                                                               AACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATA                                                                                  <  SRR19973071.2801953/150‑1 (MQ=60)
                                                                ACTTTTACATCGTGATGATTTACCTGCGGATGTCGCTGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAG                                                                                 >  SRR19973071.4314299/1‑150 (MQ=60)
                                                                  TTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCG                                                                               >  SRR19973071.1680207/1‑150 (MQ=60)
                                                                  TTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCG                                                                               >  SRR19973071.1679963/1‑150 (MQ=60)
                                                                    TTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGT                                                                             >  SRR19973071.3474886/1‑150 (MQ=60)
                                                                       CATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTT                                                                          >  SRR19973071.4094050/1‑150 (MQ=60)
                                                                       CATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTT                                                                          >  SRR19973071.4035032/1‑150 (MQ=60)
                                                                       CATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTT                                                                          >  SRR19973071.4092740/1‑150 (MQ=60)
                                                                        ATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTC                                                                         <  SRR19973071.4304155/150‑1 (MQ=60)
                                                                        ATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTC                                                                         <  SRR19973071.3902324/150‑1 (MQ=60)
                                                                         TCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGAAGTCGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCG                                                                        <  SRR19973071.2450142/150‑1 (MQ=60)
                                                                          CGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGT                                                                       >  SRR19973071.3571103/1‑150 (MQ=60)
                                                                          CGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGT                                                                       >  SRR19973071.3571099/1‑150 (MQ=60)
                                                                          CGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGT                                                                       >  SRR19973071.4142498/1‑150 (MQ=60)
                                                                          CGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGT                                                                       >  SRR19973071.874067/1‑150 (MQ=60)
                                                                            TGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTA                                                                     <  SRR19973071.92991/150‑1 (MQ=60)
                                                                             GATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTAT                                                                    >  SRR19973071.3548767/1‑150 (MQ=60)
                                                                             GATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTAT                                                                    >  SRR19973071.2407621/1‑150 (MQ=60)
                                                                             GATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTAT                                                                    >  SRR19973071.4387625/1‑150 (MQ=60)
                                                                             GATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTAT                                                                    >  SRR19973071.2407670/1‑150 (MQ=60)
                                                                                GATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAG                                                                 >  SRR19973071.4569874/1‑150 (MQ=60)
                                                                                GATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAG                                                                 <  SRR19973071.2549466/150‑1 (MQ=60)
                                                                                GATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAG                                                                 >  SRR19973071.2422880/1‑150 (MQ=60)
                                                                                 ATTTACCTGCGGATGTCGCGGAGCTGGTACTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGA                                                                >  SRR19973071.3269970/1‑150 (MQ=60)
                                                                                  TTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGAT                                                               <  SRR19973071.2736788/150‑1 (MQ=60)
                                                                                   TTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGACAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATA                                                              >  SRR19973071.490063/1‑150 (MQ=60)
                                                                                    TACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAA                                                             <  SRR19973071.4370311/150‑1 (MQ=60)
                                                                                    TACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAA                                                             <  SRR19973071.1598917/150‑1 (MQ=60)
                                                                                    TACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAA                                                             <  SRR19973071.1726365/150‑1 (MQ=60)
                                                                                    TACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAA                                                             <  SRR19973071.1726456/150‑1 (MQ=60)
                                                                                      CCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATC                                                           <  SRR19973071.930605/150‑1 (MQ=60)
                                                                                      CCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATC                                                           <  SRR19973071.1201856/150‑1 (MQ=60)
                                                                                          CGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATG                                                       <  SRR19973071.2474981/150‑1 (MQ=60)
                                                                                          CGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATG                                                       >  SRR19973071.4522018/1‑150 (MQ=60)
                                                                                          CGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATG                                                       >  SRR19973071.3180775/1‑150 (MQ=60)
                                                                                          CGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATG                                                       >  SRR19973071.3180778/1‑150 (MQ=60)
                                                                                          CGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATG                                                       >  SRR19973071.426152/1‑150 (MQ=60)
                                                                                           GGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGT                                                      >  SRR19973071.1456609/1‑150 (MQ=60)
                                                                                           GGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGT                                                      >  SRR19973071.1456612/1‑150 (MQ=60)
                                                                                            GATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTC                                                     >  SRR19973071.1748637/1‑150 (MQ=60)
                                                                                            GATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTC                                                     >  SRR19973071.1748622/1‑150 (MQ=60)
                                                                                             ATGTCGCGGAGCTGGTGCTGTATCATCTATGTGATACCCGAGCCATCATGCATATATTCAATGACGACGGGTGTTTTAGGTGGAAAGAGATCCCTGAGTTGTTGCAGGCATTTGTCTTTAGCGTTTTTCGTTATCAGATAATCGATGTCA                                                    <  SRR19973071.2466651/150‑1 (MQ=60)
                                                                                                TCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAA                                                 <  SRR19973071.4682282/150‑1 (MQ=60)
                                                                                                    GGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAT                                             >  SRR19973071.4040032/1‑150 (MQ=60)
                                                                                                     GAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATG                                            <  SRR19973071.3922897/150‑1 (MQ=60)
                                                                                                       GCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCC                                          <  SRR19973071.2678377/150‑1 (MQ=60)
                                                                                                          GGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT                                       <  SRR19973071.1819298/150‑1 (MQ=60)
                                                                                                             GCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGG                                    >  SRR19973071.4305498/1‑150 (MQ=60)
                                                                                                             GCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGG                                    >  SRR19973071.4079246/1‑150 (MQ=60)
                                                                                                                 TATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGC                                >  SRR19973071.850881/1‑150 (MQ=60)
                                                                                                                   TCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGT                              >  SRR19973071.1752910/1‑150 (MQ=60)
                                                                                                                       CAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACC                          <  SRR19973071.3625691/150‑1 (MQ=60)
                                                                                                                       CAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACC                          <  SRR19973071.3625712/150‑1 (MQ=60)
                                                                                                                       CAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACC                          <  SRR19973071.3625844/150‑1 (MQ=60)
                                                                                                                          TGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCATG                       <  SRR19973071.227533/150‑1 (MQ=60)
                                                                                                                          TGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAG                       <  SRR19973071.1509096/150‑1 (MQ=60)
                                                                                                                           GGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGA                      >  SRR19973071.4639104/1‑150 (MQ=60)
                                                                                                                               TACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTG                  >  SRR19973071.2882267/1‑150 (MQ=60)
                                                                                                                                 CCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCT                <  SRR19973071.4362101/150‑1 (MQ=60)
                                                                                                                                 CCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCT                <  SRR19973071.4362076/150‑1 (MQ=60)
                                                                                                                                  CCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTT               <  SRR19973071.552560/150‑1 (MQ=60)
                                                                                                                                  CCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTT               <  SRR19973071.640138/150‑1 (MQ=60)
                                                                                                                                     AGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTG            <  SRR19973071.2783921/150‑1 (MQ=60)
                                                                                                                                               CATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCAC  >  SRR19973071.869771/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                
CGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCAC  >  CP009273/463031‑463323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑