Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,667,443 T→G 26.6% T106P (ACC→CCC)  mdtJ ← multidrug efflux system transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,667,4430TG26.6% 387.1 / 270.1 287T106P (ACC→CCC) mdtJmultidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base T (111/100);  new base G (43/33);  total (154/133)
Fisher's exact test for biased strand distribution p-value = 5.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

Only 100 of 288 total aligned reads displayed.

CAAATATTTTGCGACGAAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGG  >  CP009273/1667295‑1667592
                                                                                                                                                    |                                                                                                                                                     
cAAATATTTTGCGACGAAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGCTTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTa                                                                                                                                                      <  2:2288395/150‑1 (MQ=255)
 aaaTATTTTGCGACGAAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGAc                                                                                                                                                     <  2:2587487/150‑1 (MQ=255)
  aaTATTTTGCGACGAAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTAcc                                                                                                                                                    <  1:875980/150‑1 (MQ=255)
  aaTATTTTGCGACGAAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTAcc                                                                                                                                                    <  1:3194519/150‑1 (MQ=255)
  aaTATTTTGCGACGAAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTAcc                                                                                                                                                    >  2:1998292/1‑150 (MQ=255)
    tatTTTGCGACGAAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTg                                                                                                                                                  >  2:354576/1‑150 (MQ=255)
        ttGCGACGAAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGAttt                                                                                                                                              <  2:3178916/150‑1 (MQ=255)
         tGCGACGAAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGAtttt                                                                                                                                             <  1:2406184/150‑1 (MQ=255)
           cgacgaAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTAt                                                                                                                                           <  2:2666948/150‑1 (MQ=255)
              cgaAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCaa                                                                                                                                        >  1:2782167/1‑150 (MQ=255)
               gaAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAac                                                                                                                                       >  1:1318125/1‑150 (MQ=255)
                     cGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATc                                                                                                                                 >  2:2507027/1‑150 (MQ=255)
                     cGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATc                                                                                                                                 >  1:3901597/1‑150 (MQ=255)
                        cAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCg                                                                                                                              >  2:3362255/1‑150 (MQ=255)
                        cAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCg                                                                                                                              <  1:2648005/150‑1 (MQ=255)
                        cAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATCCCg                                                                                                                              >  2:1401313/1‑150 (MQ=255)
                          gAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGc                                                                                                                            <  1:199574/150‑1 (MQ=255)
                           aaaaTTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCg                                                                                                                           <  2:838523/150‑1 (MQ=255)
                            aaaTTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGa                                                                                                                          >  2:1667272/1‑150 (MQ=255)
                             aaTTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGAc                                                                                                                         >  1:2969939/1‑150 (MQ=255)
                             aaTTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGAc                                                                                                                         >  1:332926/1‑150 (MQ=255)
                              aTTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGAcc                                                                                                                        <  1:414882/150‑1 (MQ=255)
                                 tCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAgg                                                                                                                     >  2:1344422/1‑150 (MQ=255)
                                 tCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATCCCGGCGACCAgg                                                                                                                     >  1:1161963/1‑150 (MQ=255)
                                     aaaGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGggtgg                                                                                                                 >  2:4088322/1‑150 (MQ=255)
                                     aaaGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGggtgg                                                                                                                 >  2:1764367/1‑150 (MQ=255)
                                       aGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGtt                                                                                                               >  1:3540256/1‑150 (MQ=255)
                                        gACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTa                                                                                                              >  2:4414558/1‑150 (MQ=255)
                                          cGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAAc                                                                                                            >  1:2382845/1‑150 (MQ=255)
                                           gTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAAcc                                                                                                           >  2:1023133/1‑150 (MQ=255)
                                             tAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCg                                                                                                         >  1:676422/1‑150 (MQ=255)
                                             tAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCg                                                                                                         >  1:1882927/1‑150 (MQ=255)
                                              aGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCgg                                                                                                        >  1:3382792/1‑150 (MQ=255)
                                               gCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGc                                                                                                       >  1:601797/1‑150 (MQ=255)
                                                cAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCCTTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCa                                                                                                      <  1:79002/150‑1 (MQ=255)
                                                cAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCa                                                                                                      <  2:125297/150‑1 (MQ=255)
                                                 aaCGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCaa                                                                                                     >  2:1412925/1‑150 (MQ=255)
                                                 aaCGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCaa                                                                                                     <  1:4052267/150‑1 (MQ=255)
                                                     aTTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAAtttt                                                                                                 <  2:4596015/150‑1 (MQ=255)
                                                     aTTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAAtttt                                                                                                 >  1:206587/1‑150 (MQ=255)
                                                       ttCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTtca                                                                                               >  1:2472186/1‑150 (MQ=255)
                                                        tCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTtcat                                                                                              >  2:2118776/1‑150 (MQ=255)
                                                          cAGCCCGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACACTCCCGGCGACCAGGGTGGTTAACCCGGCAATTTtcatca                                                                                            <  2:4154240/150‑1 (MQ=255)
                                                          cAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTtcatca                                                                                            >  1:1107291/1‑150 (MQ=255)
                                                           aGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAg                                                                                           >  2:4615810/1‑150 (MQ=255)
                                                            gCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGc                                                                                          >  2:3460868/1‑150 (MQ=255)
                                                             cACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCg                                                                                         <  2:2379871/150‑1 (MQ=255)
                                                               cGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGAt                                                                                       >  1:4099440/1‑150 (MQ=255)
                                                                  ttGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATaaa                                                                                    <  1:2123058/150‑1 (MQ=255)
                                                                   tGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAAc                                                                                   >  1:3124659/1‑150 (MQ=255)
                                                                     ccAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACtt                                                                                 <  1:529802/150‑1 (MQ=255)
                                                                      cAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACttt                                                                                <  1:289399/150‑1 (MQ=255)
                                                                      cAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACttt                                                                                <  2:1861032/150‑1 (MQ=255)
                                                                         tGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTtcgt                                                                             <  1:3816104/150‑1 (MQ=255)
                                                                          gccagccaGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTtcgtc                                                                            <  1:4254072/150‑1 (MQ=255)
                                                                          gccagccaGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTtcgtc                                                                            <  2:1130403/150‑1 (MQ=255)
                                                                           ccagccagGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTtcgtcg                                                                           <  1:1879761/150‑1 (MQ=255)
                                                                             agccagGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGaa                                                                         >  1:1745504/1‑150 (MQ=255)
                                                                                cagGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATaa                                                                      <  1:4103241/150‑1 (MQ=255)
                                                                                cagGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTAAGCGCTTTACGGGGACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATaa                                                                      >  2:4320032/1‑150 (MQ=255)
                                                                                     ggcgTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACaaaa                                                                 <  2:1304995/150‑1 (MQ=255)
                                                                                      gcgTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAAc                                                                <  2:79233/150‑1 (MQ=255)
                                                                                        gTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGc                                                              >  1:3416378/1‑150 (MQ=255)
                                                                                         tGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCt                                                             <  2:2938526/150‑1 (MQ=255)
                                                                                          gAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTa                                                            >  1:2925337/1‑150 (MQ=255)
                                                                                           aaCCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTaa                                                           <  1:454341/150‑1 (MQ=255)
                                                                                           aaCCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTaa                                                           <  1:276033/150‑1 (MQ=255)
                                                                                             cccATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAAc                                                         >  1:3257945/1‑150 (MQ=255)
                                                                                              ccATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACa                                                        >  2:3022914/1‑150 (MQ=255)
                                                                                                 ttCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAgg                                                     >  2:2362205/1‑150 (MQ=255)
                                                                                                   cAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGta                                                   <  2:685316/150‑1 (MQ=255)
                                                                                                      aCTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGtaata                                                <  1:4615753/150‑1 (MQ=255)
                                                                                                       cTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGtaataa                                               <  1:679529/150‑1 (MQ=255)
                                                                                                       cTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGtaataa                                               <  2:489012/150‑1 (MQ=255)
                                                                                                          cgcCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTaataaata                                            >  1:2269482/1‑150 (MQ=255)
                                                                                                           gcCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAataaataa                                           <  2:2749696/150‑1 (MQ=255)
                                                                                                             cATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATaaataaaa                                         <  2:2122973/150‑1 (MQ=255)
                                                                                                              aTGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATaaataaaat                                        >  1:2876618/1‑150 (MQ=255)
                                                                                                               tGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATaaataaaata                                       <  2:700214/150‑1 (MQ=255)
                                                                                                                 gTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATAcc                                     >  2:1540654/1‑150 (MQ=255)
                                                                                                                  ttCACCTCCATTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCg                                    <  1:3724645/150‑1 (MQ=255)
                                                                                                                   tCACCTCCAGTTCAGGTTTACGCGCTTTACGGGGACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGa                                   <  1:898481/150‑1 (MQ=255)
                                                                                                                   tCACCGCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGa                                   >  2:3329276/1‑150 (MQ=255)
                                                                                                                    cACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGAt                                  <  1:4411280/150‑1 (MQ=255)
                                                                                                                    cACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGAt                                  <  1:2024152/150‑1 (MQ=255)
                                                                                                                       cTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATAcc                               >  1:1021455/1‑150 (MQ=255)
                                                                                                                        tCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCt                              >  2:932188/1‑150 (MQ=255)
                                                                                                                          cAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTc                            >  1:1880972/1‑150 (MQ=255)
                                                                                                                          cAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTc                            <  2:2308367/150‑1 (MQ=255)
                                                                                                                            gTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTccc                          >  2:2643967/1‑150 (MQ=255)
                                                                                                                               cAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCcaca                       >  2:1474104/1‑150 (MQ=255)
                                                                                                                                aGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAg                      <  1:1712130/150‑1 (MQ=255)
                                                                                                                                  gTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAgcg                    >  2:3738326/1‑150 (MQ=255)
                                                                                                                                  gTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAgcg                    >  1:1398469/1‑150 (MQ=255)
                                                                                                                                         cgcTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGc             <  2:4526029/150‑1 (MQ=255)
                                                                                                                                          gcTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCt            >  2:328054/1‑150 (MQ=255)
                                                                                                                                           cTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTa           >  1:4479572/1‑150 (MQ=255)
                                                                                                                                            tttACGGGGACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTAc          >  2:1622728/1‑150 (MQ=255)
                                                                                                                                                cGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCt      <  1:4370075/150‑1 (MQ=255)
                                                                                                                                                 gggTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTa     >  2:2466065/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAAATATTTTGCGACGAAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGG  >  CP009273/1667295‑1667592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A