Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,690,981 G→T 15.0% A110E (GCG→GAG)  uidR ← transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,690,9810GT15.0% 805.7 / 84.2 247A110E (GCG→GAG) uidRtranscriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (113/97);  new base T (15/22);  total (128/119)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 248 total aligned reads displayed.

AGTAAACGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCC  >  CP009273/1690834‑1691130
                                                                                                                                                   |                                                                                                                                                     
  tAAACGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCAt                                                                                                                                                   <  2:4030878/150‑1 (MQ=255)
   aaaCGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATa                                                                                                                                                  <  2:617223/150‑1 (MQ=255)
   aaaCGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTACCGCCATa                                                                                                                                                  >  2:1100015/1‑150 (MQ=255)
      cGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAAtt                                                                                                                                               <  1:4431643/150‑1 (MQ=255)
          aaGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCaa                                                                                                                                           >  1:2739855/1‑150 (MQ=255)
                 tGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAg                                                                                                                                    >  2:1584560/1‑150 (MQ=255)
                  gCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAgc                                                                                                                                   >  2:2618285/1‑150 (MQ=255)
                        ttGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGt                                                                                                                             <  2:223723/150‑1 (MQ=255)
                         tGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGtt                                                                                                                            <  2:2432125/150‑1 (MQ=255)
                              tCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCa                                                                                                                       >  1:93048/1‑150 (MQ=255)
                                tGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCaaa                                                                                                                     <  1:2046875/150‑1 (MQ=255)
                                  ggCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAgg                                                                                                                   >  2:364177/1‑150 (MQ=255)
                                  ggCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAgg                                                                                                                   >  2:4080650/1‑150 (MQ=255)
                                   gCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGc                                                                                                                  >  1:2660049/1‑150 (MQ=255)
                                        tctcGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCAt                                                                                                             >  1:4559117/1‑150 (MQ=255)
                                         ctcGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATg                                                                                                            >  1:3802129/1‑150 (MQ=255)
                                           cGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATggg                                                                                                          <  2:1881546/150‑1 (MQ=255)
                                            gCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGt                                                                                                         <  2:1726266/150‑1 (MQ=255)
                                            gCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGt                                                                                                         >  1:1603912/1‑150 (MQ=255)
                                             ccTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTg                                                                                                        <  2:3205290/150‑1 (MQ=255)
                                              cTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTga                                                                                                       >  1:2810842/1‑150 (MQ=255)
                                               tttttGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgag                                                                                                      >  1:852622/1‑150 (MQ=255)
                                                 tttGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgagag                                                                                                    <  2:361045/150‑1 (MQ=255)
                                                  ttGCTGTGCATCACGCATACGCTGGGCAACAAATTCAGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCACTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgagaga                                                                                                   <  2:1226727/150‑1 (MQ=255)
                                                  ttGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgagaga                                                                                                   >  2:1344717/1‑150 (MQ=255)
                                                   tGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgagagag                                                                                                  >  1:2544668/1‑150 (MQ=255)
                                                    gCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgagagaga                                                                                                 >  1:1086465/1‑150 (MQ=255)
                                                       gggCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACaa                                                                                              >  1:575179/1‑150 (MQ=255)
                                                         gCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATg                                                                                            <  1:1463043/150‑1 (MQ=255)
                                                           aTCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGa                                                                                          >  2:1708201/1‑150 (MQ=255)
                                                            tCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGAc                                                                                         >  2:2161389/1‑150 (MQ=255)
                                                             cACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACt                                                                                        <  2:2736385/150‑1 (MQ=255)
                                                              aCGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTc                                                                                       <  2:1448502/150‑1 (MQ=255)
                                                               cGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCg                                                                                      <  2:4357037/150‑1 (MQ=255)
                                                                  aTCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGAcc                                                                                   >  2:3302108/1‑150 (MQ=255)
                                                                   tCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCa                                                                                  >  1:3112622/1‑150 (MQ=255)
                                                                   tCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCa                                                                                  >  1:4219071/1‑150 (MQ=255)
                                                                     cGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCata                                                                                <  1:3485468/150‑1 (MQ=255)
                                                                      gCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCatat                                                                               <  1:1698016/150‑1 (MQ=255)
                                                                           gCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCa                                                                          <  2:4537371/150‑1 (MQ=255)
                                                                           gCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCa                                                                          <  1:1259550/150‑1 (MQ=255)
                                                                            caacaaATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCaa                                                                         >  1:3504241/1‑150 (MQ=255)
                                                                            caacaaATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCaa                                                                         <  1:567578/150‑1 (MQ=255)
                                                                             aacaaATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAAc                                                                        >  2:3769703/1‑150 (MQ=255)
                                                                               caaATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGa                                                                      >  1:3154550/1‑150 (MQ=255)
                                                                                  aTTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAAt                                                                   >  1:103046/1‑150 (MQ=255)
                                                                                   ttCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATg                                                                  >  2:1425735/1‑150 (MQ=255)
                                                                                   ttCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATg                                                                  <  2:3499243/150‑1 (MQ=255)
                                                                                    tCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGa                                                                 >  1:3444267/1‑150 (MQ=255)
                                                                                    tCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGa                                                                 >  1:3915770/1‑150 (MQ=255)
                                                                                    tCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGa                                                                 <  2:1290932/150‑1 (MQ=255)
                                                                                     ccGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGaa                                                                >  1:1293130/1‑150 (MQ=255)
                                                                                      cGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAAt                                                               <  1:1001853/150‑1 (MQ=255)
                                                                                       gTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATc                                                              >  2:2896508/1‑150 (MQ=255)
                                                                                          aTCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCt                                                           <  2:1548379/150‑1 (MQ=255)
                                                                                           tCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCtt                                                          <  1:140790/150‑1 (MQ=255)
                                                                                            cGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTc                                                         <  1:3992645/150‑1 (MQ=255)
                                                                                               cATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAAt                                                      <  1:3393775/150‑1 (MQ=255)
                                                                                               cATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAAt                                                      <  2:3549652/150‑1 (MQ=255)
                                                                                                atatGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATc                                                     <  2:3285378/150‑1 (MQ=255)
                                                                                                atatGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATc                                                     >  2:4139207/1‑150 (MQ=255)
                                                                                                 tatGCTTATTTTTATGCATGGCGGCGACCTGTGGTTTACGCATCCCTTCCGCCATAATTTAATCCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGTCCATATAGTCAACGAAATGAATCCCTTCAATCg                                                    <  1:2531157/150‑1 (MQ=255)
                                                                                                   tGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGt                                                  <  1:601425/150‑1 (MQ=255)
                                                                                                     cTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCCCCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTc                                                <  2:2357263/150‑1 (MQ=255)
                                                                                                        aTTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCg                                             >  1:2913942/1‑150 (MQ=255)
                                                                                                         tttttAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTccctg                                            >  1:851717/1‑148 (MQ=255)
                                                                                                          ttttAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGa                                           >  1:2463632/1‑150 (MQ=255)
                                                                                                           tttAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGaa                                          <  2:3107893/150‑1 (MQ=255)
                                                                                                           tttAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGaa                                          <  1:1541729/150‑1 (MQ=255)
                                                                                                             tAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAAc                                        <  1:953962/150‑1 (MQ=255)
                                                                                                             tAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAAc                                        <  1:817227/150‑1 (MQ=255)
                                                                                                                gCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTACAATCGGTTCCCGGAAACggg                                     >  2:657852/1‑150 (MQ=255)
                                                                                                                 cATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGc                                    >  1:190879/1‑150 (MQ=255)
                                                                                                                   tGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCCCCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCa                                  <  2:1937208/150‑1 (MQ=255)
                                                                                                                   tGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCa                                  <  1:1749992/150‑1 (MQ=255)
                                                                                                                   tGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCa                                  >  1:739252/1‑150 (MQ=255)
                                                                                                                   tGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCa                                  >  1:3857338/1‑150 (MQ=255)
                                                                                                                   tGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCa                                  <  1:761874/150‑1 (MQ=255)
                                                                                                                   tGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCa                                  <  2:170100/150‑1 (MQ=255)
                                                                                                                   tGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCa                                  <  1:363953/150‑1 (MQ=255)
                                                                                                                    ggcggcGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAg                                 >  2:2810239/1‑150 (MQ=255)
                                                                                                                    ggcggcGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAg                                 >  2:340495/1‑150 (MQ=255)
                                                                                                                       ggcgACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAgcgc                              >  2:2323667/1‑150 (MQ=255)
                                                                                                                          gACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCct                           >  2:1220650/1‑150 (MQ=255)
                                                                                                                          gACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCct                           >  1:4351168/1‑150 (MQ=255)
                                                                                                                          gACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCct                           >  1:1997217/1‑150 (MQ=255)
                                                                                                                           aCCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCctc                          <  2:3457438/150‑1 (MQ=255)
                                                                                                                            ccTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCctct                         <  1:385235/150‑1 (MQ=255)
                                                                                                                             cTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCtctc                        >  2:2477436/1‑150 (MQ=255)
                                                                                                                              tgtgGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTcc                       >  2:546179/1‑150 (MQ=255)
                                                                                                                               gtgGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCt                      <  2:2281602/150‑1 (MQ=255)
                                                                                                                               gtgGGTTACGAATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGTGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCATTCAATCGGTTCCCGGAAACGGGACAGCGCCCTCTCCt                      >  2:87897/1‑150 (MQ=255)
                                                                                                                                  ggTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGt                   <  1:3556929/150‑1 (MQ=255)
                                                                                                                                   gTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTc                  >  1:3784186/1‑150 (MQ=255)
                                                                                                                                     tACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCt                <  2:1649797/150‑1 (MQ=255)
                                                                                                                                         cATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTaa            >  2:2654385/1‑150 (MQ=255)
                                                                                                                                            cccTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGAt         <  1:1366876/150‑1 (MQ=255)
                                                                                                                                                tCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCTAAGGTTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATc     <  1:3107069/150‑1 (MQ=255)
                                                                                                                                                tCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATc     <  1:2101856/150‑1 (MQ=255)
                                                                                                                                                tCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATc     >  2:188063/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AGTAAACGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCC  >  CP009273/1690834‑1691130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A