Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,849,550 C→A 37.2% G227C (GGC→TGC)  ydjG ← alpha‑Keto reductase, NADH‑dependent; can use methylglyoxal as substrate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,849,5500CA37.2% 356.7 / 250.7 244G227C (GGC→TGC) ydjGalpha‑Keto reductase, NADH‑dependent; can use methylglyoxal as substrate
Reads supporting (aligned to +/- strand):  ref base C (68/85);  new base A (43/48);  total (111/133)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

CAGTGCCAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCG  >  CP009273/1849401‑1849698
                                                                                                                                                     |                                                                                                                                                    
 aGTGCCAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCg                                                                                                                                                     <  1:2353110/150‑1 (MQ=255)
    gCCAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTc                                                                                                                                                  >  1:4601744/1‑150 (MQ=255)
    gCCAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTc                                                                                                                                                  <  2:3644080/150‑1 (MQ=255)
     ccAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTca                                                                                                                                                 <  1:684002/150‑1 (MQ=255)
         agTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGACCGTAATCACGAGTGATGGTGCAGGTcaacaa                                                                                                                                             >  2:2491435/1‑150 (MQ=255)
           tGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATc                                                                                                                                           <  2:4568621/150‑1 (MQ=255)
              gAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCt                                                                                                                                        >  2:774101/1‑150 (MQ=255)
              gAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCt                                                                                                                                        <  1:4211825/150‑1 (MQ=255)
              gAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCt                                                                                                                                        <  1:914648/150‑1 (MQ=255)
                 ttGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGct                                                                                                                                     <  2:1929504/150‑1 (MQ=255)
                  tgtgCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGctc                                                                                                                                    >  2:4120075/1‑150 (MQ=255)
                    tgCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTa                                                                                                                                  <  2:1919891/150‑1 (MQ=255)
                    tgCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTa                                                                                                                                  <  1:3135827/150‑1 (MQ=255)
                    tgCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTa                                                                                                                                  <  1:1009131/150‑1 (MQ=255)
                     gCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAg                                                                                                                                 >  2:2800677/1‑150 (MQ=255)
                     gCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAg                                                                                                                                 >  2:3807650/1‑150 (MQ=255)
                     gCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAg                                                                                                                                 >  2:3681365/1‑150 (MQ=255)
                        cTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCgg                                                                                                                              <  2:4429027/150‑1 (MQ=255)
                         tGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCggg                                                                                                                             >  1:3217832/1‑150 (MQ=255)
                           aTAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGa                                                                                                                           <  1:3942926/150‑1 (MQ=255)
                           aTAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGa                                                                                                                           >  2:3301931/1‑150 (MQ=255)
                            tAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGaa                                                                                                                          >  2:2989283/1‑150 (MQ=255)
                               cGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATaa                                                                                                                       <  1:3050534/150‑1 (MQ=255)
                                     caAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGa                                                                                                                 >  2:4312834/1‑150 (MQ=255)
                                     caAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGa                                                                                                                 <  1:1341615/150‑1 (MQ=255)
                                      aaaGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGaa                                                                                                                >  1:4392094/1‑150 (MQ=255)
                                       aaGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAAc                                                                                                               >  2:2574321/1‑150 (MQ=255)
                                        aGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAAcc                                                                                                              <  2:1136016/150‑1 (MQ=255)
                                          ttgCTGCCACTGTTCAATCATATCAATCACTTTCAGCATGTTTTCACGCTGGATCCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTTATCTCGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACcac                                                                                                            <  1:2783784/148‑1 (MQ=255)
                                          tGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACcac                                                                                                            <  2:2275052/150‑1 (MQ=255)
                                              tGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATg                                                                                                        <  1:4343372/150‑1 (MQ=255)
                                              tGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATg                                                                                                        <  2:742925/150‑1 (MQ=255)
                                                  aCTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCAt                                                                                                    <  2:3902694/150‑1 (MQ=255)
                                                     gTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTAt                                                                                                 <  2:1687515/150‑1 (MQ=255)
                                                      ttCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATc                                                                                                <  2:3340055/150‑1 (MQ=255)
                                                         aaGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACg                                                                                             >  2:3008635/1‑150 (MQ=255)
                                                          aGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGa                                                                                            >  1:4368132/1‑150 (MQ=255)
                                                          aGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGa                                                                                            <  1:4408429/150‑1 (MQ=255)
                                                          aGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGa                                                                                            >  2:3554237/1‑150 (MQ=255)
                                                                tcaatcaCTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGt                                                                                      >  1:1092911/1‑150 (MQ=255)
                                                                  aatcaCTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTgg                                                                                    >  2:255795/1‑150 (MQ=255)
                                                                   atcaCTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGgc                                                                                   >  1:3650078/1‑150 (MQ=255)
                                                                      aCTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGgcagc                                                                                >  1:3764872/1‑150 (MQ=255)
                                                                          tCAGCATGTTTTCACGCTGGAGCCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGtt                                                                            >  2:4248042/1‑150 (MQ=255)
                                                                          tCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGtt                                                                            >  2:1548040/1‑150 (MQ=255)
                                                                          tCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGtt                                                                            >  1:3311736/1‑150 (MQ=255)
                                                                           cAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTc                                                                           >  1:2047807/1‑150 (MQ=255)
                                                                            aGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGTAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCg                                                                          >  1:1596009/1‑150 (MQ=255)
                                                                            aGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCg                                                                          <  2:1402146/150‑1 (MQ=255)
                                                                              cATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGtt                                                                        <  2:4290817/150‑1 (MQ=255)
                                                                              cATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGtt                                                                        <  2:476842/150‑1 (MQ=255)
                                                                               aTGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGttt                                                                       >  2:95831/1‑150 (MQ=255)
                                                                               aTGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGttt                                                                       <  1:2568980/150‑1 (MQ=255)
                                                                                tGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGtttt                                                                      <  1:1808011/150‑1 (MQ=255)
                                                                                tGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGtttt                                                                      <  1:3631606/150‑1 (MQ=255)
                                                                                 gTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTc                                                                     >  2:1058565/1‑150 (MQ=255)
                                                                                  ttttCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTcc                                                                    <  1:1191483/150‑1 (MQ=255)
                                                                                  ttttCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTcc                                                                    <  2:2081571/150‑1 (MQ=255)
                                                                                    ttCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCAt                                                                  <  1:3091201/150‑1 (MQ=255)
                                                                                       aCGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGc                                                               <  1:210216/150‑1 (MQ=255)
                                                                                         gCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGccc                                                             >  1:3385747/1‑150 (MQ=255)
                                                                                          cTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCg                                                            <  1:284349/150‑1 (MQ=255)
                                                                                            ggAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGt                                                          <  1:1242802/150‑1 (MQ=255)
                                                                                             gAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAAGGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTc                                                         <  2:3826180/150‑1 (MQ=255)
                                                                                              aaCCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCg                                                        <  1:1333285/150‑1 (MQ=255)
                                                                                              aaCCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCg                                                        >  1:3124485/1‑150 (MQ=255)
                                                                                              aaCCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCg                                                        >  1:1500704/1‑150 (MQ=255)
                                                                                                 cAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAgg                                                     <  2:2032050/150‑1 (MQ=255)
                                                                                                 cAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAgg                                                     >  2:1858755/1‑150 (MQ=255)
                                                                                                 cAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAgg                                                     >  2:2065380/1‑150 (MQ=255)
                                                                                                     cTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATAc                                                 <  1:3944629/150‑1 (MQ=255)
                                                                                                     cTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATAc                                                 <  1:1065555/150‑1 (MQ=255)
                                                                                                      tttATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACt                                                <  1:605202/150‑1 (MQ=255)
                                                                                                         aTTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTa                                             <  1:774096/150‑1 (MQ=255)
                                                                                                         aTTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTa                                             <  2:623772/150‑1 (MQ=255)
                                                                                                                cGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGcc                                      >  2:1787841/1‑150 (MQ=255)
                                                                                                                cGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGcc                                      >  2:122296/1‑150 (MQ=255)
                                                                                                                cGAGCGACGCCCTTAACGTAATCACGAGTGATGGTGCAGGTCAACAATACCTGCTCTAGCGGGTAATAAACATGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTACGTTTACCATAGCCCGGTCGAGCATACTGTATATCGcc                                      >  2:122689/1‑150 (MQ=255)
                                                                                                                 gAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCt                                     >  2:2309446/1‑150 (MQ=255)
                                                                                                                 gAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCt                                     >  1:2486991/1‑150 (MQ=255)
                                                                                                                  aGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTg                                    <  1:4013069/150‑1 (MQ=255)
                                                                                                                     gccgccCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGaat                                 <  2:3827974/150‑1 (MQ=255)
                                                                                                                       cgccCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGaataa                               <  2:1724739/150‑1 (MQ=255)
                                                                                                                        gccCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGaataat                              <  2:1278091/150‑1 (MQ=255)
                                                                                                                         cccGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAataata                             >  1:2922588/1‑150 (MQ=255)
                                                                                                                           cGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATc                           <  2:3207341/150‑1 (MQ=255)
                                                                                                                           cGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATc                           <  2:2160022/150‑1 (MQ=255)
                                                                                                                            ggAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATcc                          >  1:274685/1‑150 (MQ=255)
                                                                                                                            ggAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATcc                          >  1:4452961/1‑150 (MQ=255)
                                                                                                                              aaCGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAg                        <  1:4242397/150‑1 (MQ=255)
                                                                                                                                    aTCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCAcc                  <  1:2642502/150‑1 (MQ=255)
                                                                                                                                     tCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGGGGATACGGTATTTCGCCTGTGTAATAGCCAGTTCACCa                 >  2:2601455/1‑150 (MQ=255)
                                                                                                                                     tCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCa                 <  2:1619862/150‑1 (MQ=255)
                                                                                                                                      cACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCat                <  2:2093363/150‑1 (MQ=255)
                                                                                                                                         gAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATAtt             <  1:2009384/150‑1 (MQ=255)
                                                                                                                                               tGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGAt       >  2:4636370/1‑150 (MQ=255)
                                                                                                                                                 gTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATAc     <  2:2185415/150‑1 (MQ=255)
                                                                                                                                                   gCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTc   >  1:3488065/1‑150 (MQ=255)
                                                                                                                                                   gCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTc   >  1:3488215/1‑150 (MQ=255)
                                                                                                                                                   gCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTc   <  1:532176/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAGTGCCAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCG  >  CP009273/1849401‑1849698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A