Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F218 I0 R1	1506	278.7	9275112	94.5%	8764980	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,862,142	C→A	46.9%	I326I (ATC→ATA)	yeaG →	protein kinase, endogenous substrate unidentified; autokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,862,142	0	C	A	46.9%	175.5 / inf	249	I326I (ATC→ATA)	yeaG	protein kinase, endogenous substrate unidentified; autokinase
Reads supporting (aligned to +/- strand): ref base C (72/60); new base A (49/68); total (121/128)
Fisher's exact test for biased strand distribution p-value = 5.67e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

ACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGTTTTTCCATTCTTTCGCGCCTGAAAG  >  CP009273/1861993‑1862287

aCCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATc                                                                                                                                                   >  1:642635/1‑150 (MQ=255)

  cGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGt                                                                                                                                                 >  2:4592681/1‑150 (MQ=255)

      aCTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAg                                                                                                                                             <  1:1995156/150‑1 (MQ=255)

      aCTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAg                                                                                                                                             >  1:3878540/1‑150 (MQ=255)

        tCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGt                                                                                                                                           <  1:3726002/150‑1 (MQ=255)

          aGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGc                                                                                                                                         >  2:936780/1‑150 (MQ=255)

          aGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGc                                                                                                                                         <  1:2643855/150‑1 (MQ=255)

           ggAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGAGCGTGTTTACATCGTGAAGGTGcc                                                                                                                                        >  1:1702288/1‑150 (MQ=255)

              aGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTa                                                                                                                                     <  1:3059701/150‑1 (MQ=255)

              aGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTa                                                                                                                                     <  1:1350369/150‑1 (MQ=255)

               ggTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAt                                                                                                                                    <  1:3906560/150‑1 (MQ=255)

               ggTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAt                                                                                                                                    <  1:3906451/150‑1 (MQ=255)

                gTAACTACAACGGGACGGAAGGTATCTCCTCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAtt                                                                                                                                   <  2:2208662/150‑1 (MQ=255)

                gTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAtt                                                                                                                                   >  1:1926891/1‑150 (MQ=255)

                gTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAtt                                                                                                                                   >  1:1927366/1‑150 (MQ=255)

                   aCTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTAttgct                                                                                                                                <  2:1433040/150‑1 (MQ=255)

                   aCTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTAttgct                                                                                                                                <  2:3275795/150‑1 (MQ=255)

                   aCTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTAttgct                                                                                                                                >  1:4536078/1‑150 (MQ=255)

                    cTACGACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTAttgctt                                                                                                                               <  1:109159/150‑1 (MQ=255)

                        aaCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTgcgc                                                                                                                           >  2:4511955/1‑150 (MQ=255)

                           gggACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCAtt                                                                                                                        <  2:2629121/150‑1 (MQ=255)

                            ggACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCAttt                                                                                                                       <  1:1362223/150‑1 (MQ=255)

                               cGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg                                                                                                                    >  2:4350284/1‑150 (MQ=255)

                               cGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCg                                                                                                                    >  2:71135/1‑150 (MQ=255)

                                 gAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa                                                                                                                  <  1:2863044/150‑1 (MQ=255)

                                 gAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa                                                                                                                  <  2:2767079/150‑1 (MQ=255)

                                 gAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa                                                                                                                  >  1:1813639/1‑150 (MQ=255)

                                  aaGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAag                                                                                                                 >  2:1859441/1‑150 (MQ=255)

                                  aaGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAag                                                                                                                 >  2:4187825/1‑150 (MQ=255)

                                  aaGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAag                                                                                                                 <  2:1192617/150‑1 (MQ=255)

                                  aaGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAag                                                                                                                 >  1:4173900/1‑150 (MQ=255)

                                    ggTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAagag                                                                                                               <  1:1801445/150‑1 (MQ=255)

                                     gTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAgaga                                                                                                              <  2:4473857/150‑1 (MQ=255)

                                      tATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGAt                                                                                                             <  1:2268667/150‑1 (MQ=255)

                                       aTCTCCGCCCTGCCGTTCAACGGGATTATTCTCACACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATc                                                                                                            >  2:3621901/1‑150 (MQ=255)

                                        tctcCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCa                                                                                                           <  2:330827/150‑1 (MQ=255)

                                        tctcCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCa                                                                                                           >  2:544285/1‑150 (MQ=255)

                                          tcCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa                                                                                                         >  1:4149479/1‑150 (MQ=255)

                                          tcCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa                                                                                                         >  2:2453167/1‑150 (MQ=255)

                                           ccGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaaa                                                                                                        <  2:2741116/150‑1 (MQ=255)

                                           ccGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaaa                                                                                                        >  1:2524380/1‑150 (MQ=255)

                                             gCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATc                                                                                                      >  1:1234155/1‑150 (MQ=255)

                                              cccTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCt                                                                                                     <  1:677258/150‑1 (MQ=255)

                                                cTGCCGTTCAACGGTATTATTCTCGCTCACTCGTACGTGTCCGATTGGGTCACTTTCAGTAATAACAAAAACAACGAAGCCTTCGTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTAc                                                                                                   <  1:1256910/150‑1 (MQ=255)

                                                cTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTAc                                                                                                   <  1:58140/150‑1 (MQ=255)

                                                    cGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGTTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga                                                                                               >  1:3831763/1‑150 (MQ=255)

                                                    cGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga                                                                                               >  1:4233949/1‑150 (MQ=255)

                                                     gTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGaa                                                                                              >  1:1151826/1‑150 (MQ=255)

                                                      ttCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGaaa                                                                                             >  2:4160670/1‑150 (MQ=255)

                                                      ttCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGaaa                                                                                             >  1:489338/1‑150 (MQ=255)

                                                      ttCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTGGCGCATTTCCGAAGAGATCAAAATCTACGAGaaa                                                                                             >  1:1061848/1‑150 (MQ=255)

                                                        cAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAAtt                                                                                           <  2:4307911/150‑1 (MQ=255)

                                                         aaCGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTg                                                                                          >  2:429822/1‑150 (MQ=255)

                                                          aCGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGc                                                                                         <  1:925018/150‑1 (MQ=255)

                                                           cGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCt                                                                                        <  1:516135/150‑1 (MQ=255)

                                                             ggATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTa                                                                                      >  2:3783781/1‑150 (MQ=255)

                                                                  attCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATcac                                                                                 >  2:2614926/1‑150 (MQ=255)

                                                                    tctcGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAg                                                                               <  2:3865872/150‑1 (MQ=255)

                                                                    tctcGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAg                                                                               >  1:1423417/1‑150 (MQ=255)

                                                                        gCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGaa                                                                           <  1:866943/150‑1 (MQ=255)

                                                                                gAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCa                                                                   >  2:570391/1‑150 (MQ=255)

                                                                                 aaCGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCAc                                                                  >  1:2425248/1‑150 (MQ=255)

                                                                                    gAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGcc                                                               <  1:4635616/150‑1 (MQ=255)

                                                                                     aGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGccc                                                              >  2:2870045/1‑150 (MQ=255)

                                                                                             tGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGcc                                                      <  2:3467760/150‑1 (MQ=255)

                                                                                                gTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCt                                                   <  1:2136608/150‑1 (MQ=255)

                                                                                                gTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCt                                                   <  2:1271423/150‑1 (MQ=255)

                                                                                                gTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGACGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCt                                                   <  2:641374/150‑1 (MQ=255)

                                                                                                gTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCt                                                   <  1:4329649/150‑1 (MQ=255)

                                                                                                 tCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTg                                                  <  2:3645783/150‑1 (MQ=255)

                                                                                                    cTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCa                                               <  1:883231/150‑1 (MQ=255)

                                                                                                        ccGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCt                                           <  2:3708572/150‑1 (MQ=255)

                                                                                                          gTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCg                                         >  1:3230167/1‑150 (MQ=255)

                                                                                                           taataaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGa                                        >  1:4442573/1‑150 (MQ=255)

                                                                                                               aaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAaca                                    <  1:3372688/150‑1 (MQ=255)

                                                                                                                aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAacac                                   <  1:3653138/150‑1 (MQ=255)

                                                                                                                   aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGt                                >  1:2008806/1‑150 (MQ=255)

                                                                                                                     aacaacGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCa                              >  2:2517717/1‑150 (MQ=255)

                                                                                                                     aacaacGAAGCCTTCCTCGATAGTGTTTACATCGTGAAGGTGCAGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCa                              >  1:2780484/1‑150 (MQ=255)

                                                                                                                         acGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGtt                          <  1:3587976/150‑1 (MQ=255)

                                                                                                                          cGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGttt                         <  1:1028799/150‑1 (MQ=255)

                                                                                                                          cGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGttt                         <  2:2281628/150‑1 (MQ=255)

                                                                                                                          cGAAGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGttt                         <  1:1480541/150‑1 (MQ=255)

                                                                                                                            aaGCCTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGttttt                       <  1:2330428/150‑1 (MQ=255)

                                                                                                                              gCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGTTTTTcc                     >  2:1973805/1‑150 (MQ=255)

                                                                                                                               ccTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGTTTTTCCa                    <  2:3958828/150‑1 (MQ=255)

                                                                                                                                cTTCCTCGATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGTTTTTCCAt                   <  2:1610971/150‑1 (MQ=255)

                                                                                                                                   ccTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGTTTTTCCATTCt                >  2:3505783/1‑150 (MQ=255)

                                                                                                                                     tCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGTTTTTCCATTCttt              >  1:2075818/1‑150 (MQ=255)

                                                                                                                                       gATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGTTTTTCCATTCTTTcg            >  1:3495853/1‑150 (MQ=255)

                                                                                                                                       gATCGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGTTTTTCCATTCTTTcg            >  1:1617498/1‑150 (MQ=255)

                                                                                                                                        aTCGTGTTTACATCGTGAAGGTGCCGTATTGCTTTCGCATTTCCGAAGAGATCAAAATCTACGAGAAATATCTAAATCACAGAGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGATTATCCATTCTTTagc           >  1:2394933/1‑150 (MQ=255)

                                                                                                                                        aTCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGTTTTTCCATTCTTTcgc           >  1:3207705/1‑150 (MQ=255)

                                                                                                                                          cGTGTTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGTTTTTCCATTCTTTCgcgc         >  1:3694290/1‑150 (MQ=255)

                                                                                                                                            tgtTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGTTTTTCCATTCTTTCGCGCCt       >  2:3122551/1‑150 (MQ=255)

                                                                                                                                            tgtTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGTTTTTCCATTCTTTCGCGCCt       <  1:3348086/150‑1 (MQ=255)

                                                                                                                                             gtTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGTTTTTCCATTCTTTCGCGCCTg      <  1:2689630/150‑1 (MQ=255)

                                                                                                                                             gtTTACATCGTGAAGGTGCCGTACTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGTTTTTCCATTCTTTCGCGCCTg      >  2:1734791/1‑150 (MQ=255)

                                                                                                                                             gtTTACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGTTTTTCCATTCTTTCGCGCCTg      <  1:4222549/150‑1 (MQ=255)

                                                                                                                                               ttACATAGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGTTTTTCCATTCTTTCGCGCCTGaa    >  1:3649855/1‑150 (MQ=255)

ACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTGGCACGCTCGAAACACTGTCACGTTTTTCCATTCTTTCGCGCCTGAAAG  >  CP009273/1861993‑1862287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A