Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,690,981 G→T 27.5% A110E (GCG→GAG)  uidR ← transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,690,9810GT27.5% 660.1 / 213.2 304A110E (GCG→GAG) uidRtranscriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (95/124);  new base T (44/40);  total (139/165)
Fisher's exact test for biased strand distribution p-value = 1.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 305 total aligned reads displayed.

CAGTAAACGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCC  >  CP009273/1690833‑1691130
                                                                                                                                                    |                                                                                                                                                     
    aaaCGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATa                                                                                                                                                  <  2:294647/150‑1 (MQ=255)
       cGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAAtt                                                                                                                                               <  1:1949576/150‑1 (MQ=255)
        gTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAAttt                                                                                                                                              >  2:3682727/1‑150 (MQ=255)
         tGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTc                                                                                                                                             <  2:2467322/150‑1 (MQ=255)
            aGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTTCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAac                                                                                                                                          <  2:2410736/150‑1 (MQ=255)
            aGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAac                                                                                                                                          >  1:2803578/1‑150 (MQ=255)
            aGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCAGACTTTCAac                                                                                                                                          >  2:2555786/1‑150 (MQ=255)
               ctttGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCGGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATATTTTCAtccac                                                                                                                                       <  2:949682/148‑1 (MQ=255)
                 ttGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAAccacca                                                                                                                                     <  2:3953017/150‑1 (MQ=255)
                  tGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAg                                                                                                                                    >  1:299101/1‑150 (MQ=255)
                  tGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAg                                                                                                                                    >  1:2620307/1‑150 (MQ=255)
                  tGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAg                                                                                                                                    <  2:2801447/150‑1 (MQ=255)
                    ccGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAgcg                                                                                                                                  >  2:2038455/1‑150 (MQ=255)
                    ccGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAgcg                                                                                                                                  <  2:3953562/150‑1 (MQ=255)
                     cGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAgcgc                                                                                                                                 >  1:2394721/1‑150 (MQ=255)
                      gtgtTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGcc                                                                                                                                >  1:1650299/1‑150 (MQ=255)
                        gtTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCg                                                                                                                              <  2:2138014/150‑1 (MQ=255)
                          tGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGtt                                                                                                                            >  2:3162043/1‑150 (MQ=255)
                            aTGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGt                                                                                                                          <  1:2083751/150‑1 (MQ=255)
                            aTGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGt                                                                                                                          <  1:3473165/150‑1 (MQ=255)
                                  gggCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAg                                                                                                                    <  2:1665778/150‑1 (MQ=255)
                                  gggCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAg                                                                                                                    <  1:1789482/150‑1 (MQ=255)
                                        aTCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCa                                                                                                              <  1:3235057/150‑1 (MQ=255)
                                        aTCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCa                                                                                                              >  2:4748785/1‑150 (MQ=255)
                                         tctcGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCAt                                                                                                             <  1:2507599/150‑1 (MQ=255)
                                         tctcGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCAt                                                                                                             >  2:2499099/1‑150 (MQ=255)
                                         tctcGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCAt                                                                                                             <  1:967239/150‑1 (MQ=255)
                                          ctcGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATg                                                                                                            <  2:338607/150‑1 (MQ=255)
                                           tcGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATgg                                                                                                           >  2:2726609/1‑150 (MQ=255)
                                             gCCTTTTTGGTGGGCATCACGCATCCGCTGGGAAACATATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGTTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGt                                                                                                         <  1:4161084/150‑1 (MQ=255)
                                               cTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTga                                                                                                       >  2:443228/1‑150 (MQ=255)
                                                tttttGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgag                                                                                                      <  1:2764453/150‑1 (MQ=255)
                                                tttttGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgag                                                                                                      <  1:2562528/150‑1 (MQ=255)
                                                   ttGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgagaga                                                                                                   >  1:2441089/1‑150 (MQ=255)
                                                    tGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgagagag                                                                                                  <  1:229411/150‑1 (MQ=255)
                                                     gCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgagagaga                                                                                                 >  1:3984088/1‑150 (MQ=255)
                                                     gCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGATTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgagagaga                                                                                                 <  1:2671195/150‑1 (MQ=255)
                                                       tGGGCATCACGCATCCGCTGGGCAACGAATTCCGTGATCGTCATTTGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCTTAATTTCAACCACCAGCGCCCGTTGTCCATAGGCTTCATGGGTGAGAGAGACa                                                                                               <  2:1984333/150‑1 (MQ=255)
                                                       ggggCATCACGCTTCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATATTTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACa                                                                                               <  1:3212807/149‑1 (MQ=255)
                                                        gggCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACaa                                                                                              <  2:3500224/150‑1 (MQ=255)
                                                         ggCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAAt                                                                                             >  2:1496350/1‑150 (MQ=255)
                                                            aTCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGa                                                                                          >  1:1105073/1‑150 (MQ=255)
                                                             tCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGAc                                                                                         >  1:549520/1‑150 (MQ=255)
                                                               aCGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTc                                                                                       >  2:4544228/1‑150 (MQ=255)
                                                                  cATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGAc                                                                                    <  2:1940207/150‑1 (MQ=255)
                                                                    tCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCa                                                                                  <  2:1739694/150‑1 (MQ=255)
                                                                     ccGCTGGGCAACAAATTCCGTGATCTTCATATGCTTATTTTTAAGCTTGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCat                                                                                 <  1:492726/150‑1 (MQ=255)
                                                                     ccGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCat                                                                                 <  2:4228760/150‑1 (MQ=255)
                                                                        cTGGGAAAAAAATTCCTTTATCGTAATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCATTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCAtata                                                                              <  1:2158594/150‑1 (MQ=255)
                                                                        cTGGGAAAAAAATTCCGTGATCGTCATATGATTATTTTTAAGCTTGGCGGCGACCTGTGGGTTACGAATCCCTTCCTCCATAATTTCAACCCCCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCAtata                                                                              <  1:1199401/150‑1 (MQ=255)
                                                                         tGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAg                                                                             >  2:561454/1‑150 (MQ=255)
                                                                         tGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAg                                                                             <  1:4091217/150‑1 (MQ=255)
                                                                           ggCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTc                                                                           <  2:487907/150‑1 (MQ=255)
                                                                             caacaaATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCaa                                                                         >  2:1292841/1‑150 (MQ=255)
                                                                              aacaaATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAAc                                                                        <  2:1809674/150‑1 (MQ=255)
                                                                                caaATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGa                                                                      <  2:749281/150‑1 (MQ=255)
                                                                                caaATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGa                                                                      >  1:1460943/1‑150 (MQ=255)
                                                                                caaATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGa                                                                      <  2:471242/150‑1 (MQ=255)
                                                                                  aaTTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGaaa                                                                    >  1:227808/1‑150 (MQ=255)
                                                                                    ttCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATg                                                                  <  2:2215208/150‑1 (MQ=255)
                                                                                        gTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATc                                                              >  2:889307/1‑150 (MQ=255)
                                                                                           aTCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCt                                                           >  1:2370930/1‑150 (MQ=255)
                                                                                           aTCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCt                                                           >  1:3509414/1‑150 (MQ=255)
                                                                                            tCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCtt                                                          <  2:3996792/150‑1 (MQ=255)
                                                                                             cGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTc                                                         <  1:4673842/150‑1 (MQ=255)
                                                                                              gTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCa                                                        <  2:1711604/150‑1 (MQ=255)
                                                                                               tCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCaa                                                       <  2:1549147/150‑1 (MQ=255)
                                                                                                      cTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTc                                                <  1:1436917/150‑1 (MQ=255)
                                                                                                       ttATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTcc                                               <  1:3884566/150‑1 (MQ=255)
                                                                                                           ttttAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGa                                           <  2:986773/150‑1 (MQ=255)
                                                                                                             ttAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGaaa                                         >  2:2597502/1‑150 (MQ=255)
                                                                                                              tAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAAc                                        <  2:4093396/150‑1 (MQ=255)
                                                                                                              tAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAAc                                        <  1:3729024/150‑1 (MQ=255)
                                                                                                                 gCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACggg                                     >  1:1344449/1‑150 (MQ=255)
                                                                                                                   aTGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAACGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGcc                                   <  1:985955/150‑1 (MQ=255)
                                                                                                                   aTGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGcc                                   >  2:1774168/1‑150 (MQ=255)
                                                                                                                    tGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCa                                  <  1:4725182/150‑1 (MQ=255)
                                                                                                                       cggcgACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAgcg                               <  2:256192/150‑1 (MQ=255)
                                                                                                                        ggcgACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAgcgc                              >  2:1908814/1‑150 (MQ=255)
                                                                                                                         gcgACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGcc                             <  2:1166888/150‑1 (MQ=255)
                                                                                                                           gACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCCCCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCct                           <  2:4630246/150‑1 (MQ=255)
                                                                                                                           gACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCct                           <  1:3319986/150‑1 (MQ=255)
                                                                                                                                gtgGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCt                      <  1:2080257/150‑1 (MQ=255)
                                                                                                                                gtgGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCt                      <  1:3661206/150‑1 (MQ=255)
                                                                                                                                   ggTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGt                   <  2:327698/150‑1 (MQ=255)
                                                                                                                                   ggTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGt                   <  1:1883198/150‑1 (MQ=255)
                                                                                                                                     ttACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTcc                 >  2:2994059/1‑150 (MQ=255)
                                                                                                                                     ttACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTcc                 >  2:1968247/1‑150 (MQ=255)
                                                                                                                                      tACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCt                <  2:2237260/150‑1 (MQ=255)
                                                                                                                                        cGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGt              <  1:3086543/150‑1 (MQ=255)
                                                                                                                                         gCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTa             >  2:1217901/1‑150 (MQ=255)
                                                                                                                                         gCATCCCGTCCTCCATAATTTCACCCACCAGCGCCCGTTGTCCAACGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCGTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTa             <  1:4433048/150‑1 (MQ=255)
                                                                                                                                          cATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTaa            >  1:1137187/1‑150 (MQ=255)
                                                                                                                                            tCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGa          >  1:726327/1‑150 (MQ=255)
                                                                                                                                             cccTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGAt         >  1:925804/1‑150 (MQ=255)
                                                                                                                                              ccTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATa        <  1:1692426/150‑1 (MQ=255)
                                                                                                                                                ttCCGCCATAATTTCAACCCCCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAAt      <  2:141388/150‑1 (MQ=255)
                                                                                                                                                 tCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATc     >  1:1261058/1‑150 (MQ=255)
                                                                                                                                                   cGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGc   <  1:4296949/150‑1 (MQ=255)
                                                                                                                                                    gCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGcc  >  1:3119824/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGTAAACGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCC  >  CP009273/1690833‑1691130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A