Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,669,592 G→T 100% F342L (TTC→TTA)  pntB ← pyridine nucleotide transhydrogenase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,669,5920GT98.5% 630.4 / ‑0.5 198F342L (TTC→TTA) pntBpyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (103/92);  total (105/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 198 total aligned reads displayed.

TAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTG  >  CP009273/1669447‑1669736
                                                                                                                                                 |                                                                                                                                                
     ccAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGca                                                                                                                                           >  1:1957494/1‑148 (MQ=255)
      cAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGcac                                                                                                                                          >  1:143208/1‑148 (MQ=255)
       aaTCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGcaca                                                                                                                                         >  2:891852/1‑148 (MQ=255)
         tCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACAtt                                                                                                                                       >  2:668767/1‑148 (MQ=255)
          cACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTa                                                                                                                                      >  1:3311701/1‑148 (MQ=255)
            ccAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAAt                                                                                                                                    >  2:2095485/1‑148 (MQ=255)
                  cggtatcggtatCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGTTGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACg                                                                                                                              <  2:2112868/148‑1 (MQ=255)
                   ggtatcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGa                                                                                                                             <  2:1144203/148‑1 (MQ=255)
                     tatcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgc                                                                                                                           >  1:3519755/1‑148 (MQ=255)
                      atcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgcg                                                                                                                          >  2:3498410/1‑148 (MQ=255)
                        cggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgcgcg                                                                                                                        >  2:2824637/1‑148 (MQ=255)
                            atcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAt                                                                                                                    >  1:1044481/1‑148 (MQ=255)
                              cAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAttt                                                                                                                  >  1:14830/1‑148 (MQ=255)
                                 cAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTtctc                                                                                                               >  1:3198230/1‑148 (MQ=255)
                                 cAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTtctc                                                                                                               >  2:3334509/1‑148 (MQ=255)
                                   aaGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAg                                                                                                             >  2:3464828/1‑148 (MQ=255)
                                    aGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGt                                                                                                            >  2:2329252/1‑148 (MQ=255)
                                    aGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGt                                                                                                            >  1:1601882/1‑148 (MQ=255)
                                     gtaatcttTGATCTCGTCCATTTCCAGCACTATTTAATACGGTACTTTTGATTCAGCAAGCAATACGTTCATTTGTCCAGCAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTa                                                                                                           <  1:993267/145‑1 (MQ=255)
                                     gtaatcatTTATCTCGTCAATTTCCAGCACTATTTCTTACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGCAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTa                                                                                                           <  1:412676/145‑1 (MQ=255)
                                     gTCATCATTGTTCTCGTCCTTTTCCTGCACGATGTCATACGTTACTTTTGATTCAGCCAGCAATACTTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTa                                                                                                           <  1:2304487/148‑1 (MQ=255)
                                     gTCATCATTGATCTCGTCCATTTCCAGCACGTTGTAATACGGTACTTTTGCTTCAGCCACCAATACGTTCATAGGGCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTa                                                                                                           <  1:2901978/148‑1 (MQ=255)
                                      tcatctTTGATCTCGTCCATTTCCAGCACGATGTCATAGGGTACTTTTGCTTCAGCCAGCAATACGTTCATTTGTCCTGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTaa                                                                                                          <  1:2360889/148‑1 (MQ=255)
                                       catcatTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAt                                                                                                         <  1:2200345/148‑1 (MQ=255)
                                       catcatTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAt                                                                                                         >  1:1289124/1‑148 (MQ=255)
                                       catcatTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAt                                                                                                         >  1:271786/1‑148 (MQ=255)
                                         tcatTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAttt                                                                                                       <  2:1240030/148‑1 (MQ=255)
                                          catTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTc                                                                                                      >  2:3391817/1‑148 (MQ=255)
                                            ttGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAg                                                                                                    >  2:2897064/1‑148 (MQ=255)
                                            ttGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAg                                                                                                    <  2:683106/148‑1 (MQ=255)
                                               aTCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGa                                                                                                 >  1:2380233/1‑148 (MQ=255)
                                               aTCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGa                                                                                                 <  2:80722/148‑1 (MQ=255)
                                                tctcGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAc                                                                                                >  1:557908/1‑148 (MQ=255)
                                                tctcGTCAATTTCCAGCACGATGTCATACGTTAATTTTGCTACAGCCAGAAATACTTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAc                                                                                                <  1:860637/148‑1 (MQ=255)
                                                      ccATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGata                                                                                          >  2:2219745/1‑148 (MQ=255)
                                                       cttttCCAGCACTATGTCATACGTTACTTTTGCTTCAGCCAGCAATCCGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGatat                                                                                         <  2:2625970/146‑1 (MQ=255)
                                                       cATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGatat                                                                                         >  1:3391152/1‑148 (MQ=255)
                                                       cATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGatat                                                                                         >  1:2628295/1‑148 (MQ=255)
                                                        aTTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATAtt                                                                                        >  1:3454998/1‑148 (MQ=255)
                                                        aTTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATAtt                                                                                        >  1:2359855/1‑148 (MQ=255)
                                                          ttCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCACTTTCTCAGTAATTTCAGCGACAGGATATTgc                                                                                      >  1:2871300/1‑148 (MQ=255)
                                                          ttCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgc                                                                                      >  1:1960025/1‑148 (MQ=255)
                                                            ccaccaCGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATTTGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgcgc                                                                                    <  2:353417/148‑1 (MQ=255)
                                                            ccAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgcgc                                                                                    <  2:636921/148‑1 (MQ=255)
                                                              aGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCACTTTCTCAGTAATTTCAGCGACAGGATATTGCGCCt                                                                                  >  2:2387690/1‑148 (MQ=255)
                                                                cACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTgc                                                                                >  1:180166/1‑148 (MQ=255)
                                                                cACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTgc                                                                                >  1:2151747/1‑148 (MQ=255)
                                                                  cGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTgcgc                                                                              >  2:1646080/1‑148 (MQ=255)
                                                                  cGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTgcgc                                                                              >  2:1564786/1‑148 (MQ=255)
                                                                    aTGTAATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGa                                                                            <  2:3594012/148‑1 (MQ=255)
                                                                     tGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGAc                                                                           >  2:746556/1‑148 (MQ=255)
                                                                      gTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACt                                                                          >  2:3672468/1‑148 (MQ=255)
                                                                       tCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACCCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTg                                                                         <  2:405109/148‑1 (MQ=255)
                                                                         aTACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGcc                                                                       <  2:3627785/148‑1 (MQ=255)
                                                                          tACGTTATTTTTGCTTCCGCCAGCAATACGTTAATCTGGCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCa                                                                      <  1:891852/148‑1 (MQ=255)
                                                                          tACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCa                                                                      >  2:3012298/1‑148 (MQ=255)
                                                                            cGGTACTTTTGATTCAGCCAGCAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATg                                                                    <  1:1220679/148‑1 (MQ=255)
                                                                               tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCg                                                                 >  1:3115312/1‑148 (MQ=255)
                                                                                aCTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGt                                                                >  2:3093495/1‑148 (MQ=255)
                                                                                aCTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGt                                                                <  2:1573641/148‑1 (MQ=255)
                                                                                aCTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGt                                                                >  1:3418630/1‑148 (MQ=255)
                                                                                aCTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGt                                                                >  1:328461/1‑148 (MQ=255)
                                                                                          cagccagaAATACTTTCATTTGTCCAGGAAAACCCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGt                                                      <  2:4872/148‑1 (MQ=255)
                                                                                             ccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAt                                                   <  1:3370783/148‑1 (MQ=255)
                                                                                                 cAATACTTTCATTTGTCCTGGAAACCGCCCCGCGACCGGGTGGATACCTAAACGCACTTTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATc                                               <  1:1145397/148‑1 (MQ=255)
                                                                                                 cAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATc                                               >  2:2143106/1‑148 (MQ=255)
                                                                                                   aTACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCAc                                             >  1:3576342/1‑148 (MQ=255)
                                                                                                   aTACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCAc                                             >  2:2569290/1‑148 (MQ=255)
                                                                                                    tACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACt                                            >  2:1155882/1‑148 (MQ=255)
                                                                                                    tACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACt                                            <  2:623888/148‑1 (MQ=255)
                                                                                                    tACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACt                                            >  2:1347744/1‑148 (MQ=255)
                                                                                                              tGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGt                                  <  2:2537448/148‑1 (MQ=255)
                                                                                                              tGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGt                                  <  2:1601884/148‑1 (MQ=255)
                                                                                                               gTCCAGGCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtt                                 <  2:2252500/148‑1 (MQ=255)
                                                                                                               gTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtt                                 <  1:2329252/148‑1 (MQ=255)
                                                                                                                tCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGttt                                <  2:3820443/148‑1 (MQ=255)
                                                                                                                 ccAGGCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtttt                               <  1:3389172/148‑1 (MQ=255)
                                                                                                                  cAGGCAAACCCCCCGCGACCGGGTGGATACCTAAACGCACTTTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGttttt                              <  1:2355122/148‑1 (MQ=255)
                                                                                                                   aGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTACTGATCACTGAATGGGAGTTTTTc                             >  2:1066038/1‑148 (MQ=255)
                                                                                                                   aGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTc                             >  1:2722148/1‑148 (MQ=255)
                                                                                                                   aGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTc                             <  1:3316077/148‑1 (MQ=255)
                                                                                                                    ggCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTca                            >  2:186425/1‑148 (MQ=255)
                                                                                                                     gCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcag                           >  1:2116642/1‑148 (MQ=255)
                                                                                                                     gCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcag                           <  2:557908/148‑1 (MQ=255)
                                                                                                                      cAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcagc                          >  1:1885244/1‑148 (MQ=255)
                                                                                                                        aaCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcagcag                        <  2:2486164/148‑1 (MQ=255)
                                                                                                                         aCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGt                       >  1:570685/1‑148 (MQ=255)
                                                                                                                         aCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGt                       >  2:675813/1‑148 (MQ=255)
                                                                                                                          cGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGtt                      >  1:2422926/1‑148 (MQ=255)
                                                                                                                          cGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGtt                      >  2:1410775/1‑148 (MQ=255)
                                                                                                                           gCCCCGCGACCGGGTGGATACCTAACCGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTc                     <  2:2273034/148‑1 (MQ=255)
                                                                                                                                    ccGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGtctc            <  1:2824637/148‑1 (MQ=255)
                                                                                                                                      gggTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCtctt          <  2:2996012/148‑1 (MQ=255)
                                                                                                                                       ggTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCtcttc         <  2:3454998/148‑1 (MQ=255)
                                                                                                                                           gATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCg     >  1:37604/1‑148 (MQ=255)
                                                                                                                                            aTACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCgg    >  2:1056352/1‑148 (MQ=255)
                                                                                                                                            aTACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCgg    >  2:109945/1‑148 (MQ=255)
                                                                                                                                            aTACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCgg    >  2:1129897/1‑148 (MQ=255)
                                                                                                                                             tACCTAAACGCACATTAATACCACGAGCGCGCAAGTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGt   <  1:3785496/148‑1 (MQ=255)
                                                                                                                                              aCCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTg  <  1:3775717/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTG  >  CP009273/1669447‑1669736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTC  >  CP009273/1669450‑1669741
                                                                                                                                              |                                                                                                                                                     
CACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCA                                                                                                                                                >  SRR19973069.1971057/1‑150 (MQ=60)
 ACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCAC                                                                                                                                               >  SRR19973069.144503/1‑150 (MQ=60)
     ATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTA                                                                                                                                           >  SRR19973069.3333406/1‑150 (MQ=60)
        ACCTGTACGTTATCGTTATCAGAAAAGTCATCTTTGATCTCGTCCATTACCAGCACGATGTCATACGTTACTTTTGCTTCAGCCAGAAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATA                                                                                                                                        <  SRR19973069.1099224/150‑1 (MQ=60)
         ccagttcgttttggttatcagaaaattaATCATTGATCTCGTCATTTTCCAGCACTATTTAATACGTTTTTTTTGATTCAGCCAGAAATCCTTTAATATGTCCAGCAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATAC                                                                                                                                       <  SRR19973069.1312628/122‑1 (MQ=31)
          CAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACC                                                                                                                                      >  SRR19973069.1457488/1‑150 (MQ=60)
             TACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGAAATACGTTCATTTGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACG                                                                                                                                   <  SRR19973069.516347/150‑1 (MQ=60)
                GGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGC                                                                                                                                >  SRR19973069.3542491/1‑150 (MQ=60)
                GGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGC                                                                                                                                <  SRR19973069.3795727/150‑1 (MQ=60)
                   atcttTATCAGCAACGTCATCTTTTATATCGTCAATTTCCAGCACTTTTTAATACGTTACTTTTGCTTCAGCCAGCAATAGGTTAATTGGTCAAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCG                                                                                                                             <  SRR19973069.3247042/145‑1 (MQ=54)
                       GTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAT                                                                                                                         >  SRR19973069.1051737/1‑150 (MQ=60)
                       GTATCAGAAAAGTAATATTTGATCTCGTCCATTTCCAGCACTATTTCATACGTTACTTTTGATTCAGCCAGAAATACTTTCATAGGTCCTGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAT                                                                                                                         <  SRR19973069.3588249/150‑1 (MQ=60)
                         ATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTT                                                                                                                       >  SRR19973069.15049/1‑150 (MQ=60)
                            AGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTC                                                                                                                    >  SRR19973069.1304693/1‑150 (MQ=60)
                            AGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTC                                                                                                                    >  SRR19973069.3219023/1‑150 (MQ=60)
                              CAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAG                                                                                                                  <  SRR19973069.3146931/150‑1 (MQ=60)
                               AAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGT                                                                                                                 >  SRR19973069.1612675/1‑150 (MQ=60)
                               AAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGT                                                                                                                 >  SRR19973069.1612677/1‑150 (MQ=60)
                                  GTCATCATTGTTCTCGTCCTTTTCCTGCACGATGTCATACGTTACTTTTGATTCAGCCAGCAATACTTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAT                                                                                                              <  SRR19973069.2320159/150‑1 (MQ=60)
                                  GTCATCATTGATCTCGTCCATTTCCAGCACGTTGTAATACGGTACTTTTGCTTCAGCCACCAATACGTTCATAGGGCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAT                                                                                                              <  SRR19973069.2921148/150‑1 (MQ=60)
                                  GTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAT                                                                                                              >  SRR19973069.1297740/1‑150 (MQ=60)
                                  GTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAT                                                                                                              >  SRR19973069.273709/1‑150 (MQ=60)
                                  GTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAT                                                                                                              <  SRR19973069.2320357/150‑1 (MQ=60)
                                  GTCATAATTGATCTCGTCCATTTCCAGCACGTTGTCATACGGTACTTTTGCTTCAGCCAAAAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAT                                                                                                              <  SRR19973069.3017441/150‑1 (MQ=60)
                                  GTAATCTTTGATCTCGTCCATTTCCAGCACTATTTAATACGGTACTTTTGATTCAGCAAGCAATACGTTCATTTGTCCAGCAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAT                                                                                                              <  SRR19973069.1000017/150‑1 (MQ=60)
                                  GTAATCATTTATCTCGTCAATTTCCAGCACTATTTCTTACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGCAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAT                                                                                                              <  SRR19973069.415696/150‑1 (MQ=60)
                                   TCATCTTTGATCTCGTCCATTTCCAGCACGATGTCATAGGGTACTTTTGCTTCAGCCAGCAATACGTTCATTTGTCCTGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATT                                                                                                             <  SRR19973069.2376934/150‑1 (MQ=60)
                                    CATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTT                                                                                                            <  SRR19973069.2215279/150‑1 (MQ=60)
                                    AATCATTTATCTCGTCCATTTCCAGCACTATGTCATACGGTACTTTTGATTCACCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTT                                                                                                            <  SRR19973069.362573/150‑1 (MQ=60)
                                         TTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCG                                                                                                       <  SRR19973069.598449/150‑1 (MQ=60)
                                          TGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGA                                                                                                      >  SRR19973069.2396377/1‑150 (MQ=60)
                                           GATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAC                                                                                                     >  SRR19973069.561791/1‑150 (MQ=60)
                                           GATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAC                                                                                                     >  SRR19973069.563580/1‑150 (MQ=60)
                                             TCTCGTCAATTTCCAGCACGATGTCATACGTTAATTTTGCTACAGCCAGAAATACTTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAG                                                                                                   <  SRR19973069.866650/150‑1 (MQ=60)
                                              CTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGG                                                                                                  >  SRR19973069.2235889/1‑150 (MQ=60)
                                                  TCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATAT                                                                                              >  SRR19973069.3413301/1‑150 (MQ=60)
                                                  TCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATAT                                                                                              >  SRR19973069.2646212/1‑150 (MQ=60)
                                                  TCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATAT                                                                                              >  SRR19973069.2645891/1‑150 (MQ=60)
                                                   CCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATT                                                                                             >  SRR19973069.278016/1‑150 (MQ=60)
                                                   CCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATT                                                                                             >  SRR19973069.3477386/1‑150 (MQ=60)
                                                   CCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATT                                                                                             >  SRR19973069.2375894/1‑150 (MQ=60)
                                                   CCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATT                                                                                             >  SRR19973069.2375887/1‑150 (MQ=60)
                                                   CCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATT                                                                                             >  SRR19973069.2375711/1‑150 (MQ=60)
                                                    CTTTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTAATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTG                                                                                            <  SRR19973069.3481560/150‑1 (MQ=60)
                                                     ATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCACTTTCTCAGTAATTTCAGCGACAGGATATTGC                                                                                           >  SRR19973069.2890156/1‑150 (MQ=60)
                                                     ATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGC                                                                                           >  SRR19973069.896655/1‑150 (MQ=60)
                                                     ATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGC                                                                                           >  SRR19973069.1973604/1‑150 (MQ=60)
                                                     ATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGC                                                                                           >  SRR19973069.1973607/1‑150 (MQ=60)
                                                           AGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGC                                                                                     >  SRR19973069.181669/1‑150 (MQ=60)
                                                           AGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGC                                                                                     >  SRR19973069.2166486/1‑150 (MQ=60)
                                                           AGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTCC                                                                                     >  SRR19973069.2382766/1‑150 (MQ=60)
                                                                       TACGTTATTTTTGCTTCCGCCAGCAATACGTTAATCTGGCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATG                                                                         <  SRR19973069.898000/150‑1 (MQ=60)
                                                                         CGGTACTTTTGATTCAGCCAGCAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCC                                                                       <  SRR19973069.1229030/150‑1 (MQ=60)
                                                                          GGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCG                                                                      >  SRR19973069.3135786/1‑150 (MQ=60)
                                                                           GTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGT                                                                     >  SRR19973069.330692/1‑150 (MQ=60)
                                                                           GTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGT                                                                     >  SRR19973069.3440887/1‑150 (MQ=60)
                                                                           GTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGT                                                                     >  SRR19973069.330682/1‑150 (MQ=60)
                                                                             ACTTTTGCTTCCGCCACCAATACGTTCATATGACCCGGAAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAC                                                                   <  SRR19973069.3402130/150‑1 (MQ=60)
                                                                                    CTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAG                                                            >  SRR19973069.2168615/1‑150 (MQ=60)
                                                                                    CTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAG                                                            >  SRR19973069.3365381/1‑150 (MQ=60)
                                                                                         GCCAGCAATACGTTCATTTGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCCCGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATG                                                       <  SRR19973069.2109968/150‑1 (MQ=60)
                                                                                          CCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGA                                                      <  SRR19973069.3392824/150‑1 (MQ=60)
                                                                                           CAGCAATACGTTCATATGTCCAGGCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGAT                                                     <  SRR19973069.2153420/150‑1 (MQ=60)
                                                                                           CAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGAGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGAT                                                     >  SRR19973069.2867986/1‑150 (MQ=60)
                                                                                            AGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATC                                                    >  SRR19973069.3836542/1‑150 (MQ=60)
                                                                                              CAATACTTTCATTTGTCCTGGAAACCGCCCCGCGACCGGGTGGATACCTAAACGCACTTTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCAC                                                  <  SRR19973069.1153390/150‑1 (MQ=60)
                                                                                              CAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCAC                                                  >  SRR19973069.3599658/1‑150 (MQ=60)
                                                                                               AATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACT                                                 >  SRR19973069.2545041/1‑150 (MQ=60)
                                                                                                       CATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGA                                         >  SRR19973069.1174396/1‑150 (MQ=60)
                                                                                                            GTCCAGGCAAACGCCCCGCGACGGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTT                                    <  SRR19973069.2345098/150‑1 (MQ=60)
                                                                                                            GCCCAGGAAAACGCCCCGCGACCGGGTGGTTACCTAAACGCACATTAATACCACGAGCGCGAAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTT                                    <  SRR19973069.409786/150‑1 (MQ=60)
                                                                                                             TCCAGGCAACCGCCCCGCGACCGGGTGGATACCTAAACGCAAATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTT                                   <  SRR19973069.954846/150‑1 (MQ=60)
                                                                                                              CCAGGCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTC                                  <  SRR19973069.3411310/150‑1 (MQ=60)
                                                                                                              CCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTC                                  >  SRR19973069.2739915/1‑150 (MQ=60)
                                                                                                              CCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTC                                  >  SRR19973069.2740387/1‑150 (MQ=60)
                                                                                                              CCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTC                                  <  SRR19973069.3411364/150‑1 (MQ=60)
                                                                                                               ccgcaaacccCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCCCGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCA                                 <  SRR19973069.2275850/140‑1 (MQ=60)
                                                                                                               CAGGCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCA                                 <  SRR19973069.2371122/150‑1 (MQ=60)
                                                                                                               CAGGCAAACCCCCCGCGACCGGGTGGATACCTAAACGCACTTTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCA                                 <  SRR19973069.2371130/150‑1 (MQ=60)
                                                                                                                AGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAG                                >  SRR19973069.1286107/1‑150 (MQ=60)
                                                                                                                AGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAG                                >  SRR19973069.2131233/1‑150 (MQ=60)
                                                                                                                AGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAG                                <  SRR19973069.3336987/150‑1 (MQ=60)
                                                                                                                AGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAG                                <  SRR19973069.3337817/150‑1 (MQ=60)
                                                                                                                 GGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGC                               >  SRR19973069.1898136/1‑150 (MQ=60)
                                                                                                                 GGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGC                               >  SRR19973069.1898331/1‑150 (MQ=60)
                                                                                                                    AAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGT                            >  SRR19973069.574637/1‑150 (MQ=60)
                                                                                                                     AACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTT                           >  SRR19973069.2439283/1‑150 (MQ=60)
                                                                                                                       CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCC                         <  SRR19973069.243453/150‑1 (MQ=60)
                                                                                                                          CCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCT                      <  SRR19973069.2896113/150‑1 (MQ=60)
                                                                                                                              CGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCT                  <  SRR19973069.3431606/150‑1 (MQ=60)
                                                                                                                                ACCGGGTGGATACCTAACCGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCT                <  SRR19973069.344797/150‑1 (MQ=60)
                                                                                                                                ACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCT                <  SRR19973069.1535431/150‑1 (MQ=60)
                                                                                                                                 CCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTT               <  SRR19973069.2843318/150‑1 (MQ=60)
                                                                                                                                      TGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCG          >  SRR19973069.38112/1‑150 (MQ=60)
                                                                                                                                          TACCTAAACGCACATTAATACCACGAGCGCGCAATGTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGA      <  SRR19973069.1910080/150‑1 (MQ=60)
                                                                                                                                          TACCTAAACGCACATTAATACCACGAGCGCGCAAGTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGA      <  SRR19973069.3810057/150‑1 (MQ=60)
                                                                                                                                           ACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGAT     <  SRR19973069.3800898/150‑1 (MQ=60)
                                                                                                                                           ACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGAT     <  SRR19973069.3800245/150‑1 (MQ=60)
                                                                                                                                             CTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTTCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTT   >  SRR19973069.2369734/1‑150 (MQ=60)
                                                                                                                                              TAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTC  <  SRR19973069.3137840/150‑1 (MQ=60)
                                                                                                                                              TAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTC  <  SRR19973069.3137284/150‑1 (MQ=60)
                                                                                                                                              |                                                                                                                                                     
CACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTC  >  CP009273/1669450‑1669741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑