Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F342 I0 R1	1331	225.9	7641038	95.8%	7320114	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,852,913	C→A	42.0%	E60* (GAA→TAA)	ydjJ ←	putative Zn‑dependent NAD(P)‑binding oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,852,913	0	C	A	42.0%	262.4 / 265.3	256	E60* (GAA→TAA)	ydjJ	putative Zn‑dependent NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (72/76); new base A (49/59); total (121/135)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

AGATGTTATATTTGCCTTCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTGGTATTTCTGCTGAAATAATTTTCATTGTGCCC  >  CP009273/1852766‑1853058

  aTGTTATATTTGCCTTCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTAtt                                                                                                                                                 >  2:3274446/1‑148 (MQ=255)

  aTGTTATATTTGCCTTCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTAtt                                                                                                                                                 >  2:2506380/1‑148 (MQ=255)

  aTGTTATATTTGCCTTCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTAtt                                                                                                                                                 >  2:586486/1‑148 (MQ=255)

  aTGTTATATTTGCCTTCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTAtt                                                                                                                                                 >  2:586719/1‑148 (MQ=255)

     ttATATTTGCCTTCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGAt                                                                                                                                              <  2:2569706/148‑1 (MQ=255)

     ttATATTTGCCTTCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGAt                                                                                                                                              <  1:1342337/148‑1 (MQ=255)

     ttATATTTGCCTTCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGAt                                                                                                                                              <  2:3454254/148‑1 (MQ=255)

      tataTTTGCCTTCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGAtt                                                                                                                                             >  2:2521992/1‑148 (MQ=255)

      tataTTTGCCTTCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGAtt                                                                                                                                             <  1:220287/148‑1 (MQ=255)

       atatTTGCCTTCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGAttt                                                                                                                                            >  2:285714/1‑148 (MQ=255)

            tGCCTTCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTc                                                                                                                                       <  2:3048315/148‑1 (MQ=255)

              ccTTCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCtt                                                                                                                                     >  2:1069351/1‑148 (MQ=255)

               cTTCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCttt                                                                                                                                    <  1:1450939/148‑1 (MQ=255)

                ttCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTa                                                                                                                                   <  1:2346378/148‑1 (MQ=255)

                ttCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGt                                                                                                                                        >  2:3499474/1‑143 (MQ=255)

                 tCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCACATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAg                                                                                                                                  >  1:3462262/1‑148 (MQ=255)

                  ccAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAgg                                                                                                                                 >  2:2031102/1‑148 (MQ=255)

                  ccAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAgg                                                                                                                                 <  2:436674/148‑1 (MQ=255)

                      actgataCGACTGTGACCGCAAGGAACGCCAGGTTCTATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGa                                                                                                                             <  1:451913/142‑1 (MQ=255)

                      aCAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGa                                                                                                                             >  1:463024/1‑148 (MQ=255)

                      aCAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGa                                                                                                                             <  2:2488372/148‑1 (MQ=255)

                      aCAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGa                                                                                                                             <  2:2195705/148‑1 (MQ=255)

                       cAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGaa                                                                                                                            <  2:2969021/148‑1 (MQ=255)

                          tAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATaa                                                                                                                         >  2:3157333/1‑148 (MQ=255)

                          tAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATaa                                                                                                                         >  2:2271145/1‑148 (MQ=255)

                           aaCGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATaaa                                                                                                                        <  1:3232396/148‑1 (MQ=255)

                               aCAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACggg                                                                                                                    <  1:3058281/148‑1 (MQ=255)

                               aCAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACggg                                                                                                                    <  1:1550192/148‑1 (MQ=255)

                                cAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGc                                                                                                                   >  1:730203/1‑148 (MQ=255)

                                 aGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGcc                                                                                                                  >  2:3090257/1‑148 (MQ=255)

                                 aGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCAGTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGcc                                                                                                                  >  2:2597556/1‑148 (MQ=255)

                                   tGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTg                                                                                                                <  2:704962/148‑1 (MQ=255)

                                    gACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGa                                                                                                               >  1:1616408/1‑148 (MQ=255)

                                    gACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGa                                                                                                               <  1:2747690/148‑1 (MQ=255)

                                    gACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGa                                                                                                               <  1:530745/148‑1 (MQ=255)

                                          aaGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCaaa                                                                                                         <  2:2532369/148‑1 (MQ=255)

                                            ggAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAAc                                                                                                       >  1:3131459/1‑148 (MQ=255)

                                             gAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAAcc                                                                                                      >  1:2189463/1‑148 (MQ=255)

                                                   cAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTAc                                                                                                >  2:1350796/1‑148 (MQ=255)

                                                   cAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTAc                                                                                                <  2:2821729/148‑1 (MQ=255)

                                                    aGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACa                                                                                               >  2:3578337/1‑148 (MQ=255)

                                                      gTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATc                                                                                             >  1:2275940/1‑148 (MQ=255)

                                                       ttCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCTAAACCAGGTACATCt                                                                                            >  1:3509208/1‑148 (MQ=255)

                                                       ttCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCt                                                                                            >  1:3508848/1‑148 (MQ=255)

                                                       ttCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCt                                                                                            <  2:3692326/148‑1 (MQ=255)

                                                       ttCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTGTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTAAATCt                                                                                            >  1:2564170/1‑148 (MQ=255)

                                                         cGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGa                                                                                          <  2:2365161/148‑1 (MQ=255)

                                                           atatTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAAc                                                                                        >  1:825222/1‑148 (MQ=255)

                                                             atTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACca                                                                                      >  2:810571/1‑148 (MQ=255)

                                                               ttACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACcaca                                                                                    >  2:918770/1‑148 (MQ=255)

                                                                tACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACaa                                                                                   <  1:1110518/148‑1 (MQ=255)

                                                                  cccGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAAt                                                                                 <  2:463024/148‑1 (MQ=255)

                                                                  cccGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAAt                                                                                 <  1:2521992/148‑1 (MQ=255)

                                                                  cccGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAAt                                                                                 <  2:2383822/148‑1 (MQ=255)

                                                                   ccGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATa                                                                                <  1:2405248/148‑1 (MQ=255)

                                                                    cGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATAc                                                                               <  1:934373/148‑1 (MQ=255)

                                                                      aTCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCg                                                                             >  1:1011097/1‑148 (MQ=255)

                                                                      aTCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCg                                                                             >  1:3602185/1‑148 (MQ=255)

                                                                       tCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGa                                                                            >  2:1880138/1‑148 (MQ=255)

                                                                            cGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACatat                                                                       <  1:2535581/148‑1 (MQ=255)

                                                                            cGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACatat                                                                       >  2:3402325/1‑148 (MQ=255)

                                                                              gTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTc                                                                     <  1:2491387/148‑1 (MQ=255)

                                                                               tttAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCt                                                                    >  2:176239/1‑148 (MQ=255)

                                                                                 tAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTAc                                                                  <  1:1870576/148‑1 (MQ=255)

                                                                                      ttGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTaa                                                             >  2:3147673/1‑148 (MQ=255)

                                                                                       tGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAAt                                                            <  2:662666/148‑1 (MQ=255)

                                                                                            aCGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCaaaa                                                       <  1:2036173/148‑1 (MQ=255)

                                                                                                 gCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCa                                                  <  2:2800530/148‑1 (MQ=255)

                                                                                                 gCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCa                                                  >  1:3342276/1‑148 (MQ=255)

                                                                                                  cTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAACTACCGACATATTCTACTTTAATCAAAACTTCAt                                                 <  2:1086930/148‑1 (MQ=255)

                                                                                                  cTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCAt                                                 <  1:3697601/148‑1 (MQ=255)

                                                                                                     cccACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCAtctt                                              <  1:3590465/148‑1 (MQ=255)

                                                                                                      cgacaGCCACATCCGTGCCGGCGCTTTCTTGACCCAGGGCAATTTCTTGATTTGGGTCTTTAGGCGGATTAAACGGGCCTGATTCAAAACCATGTTCATCTGATCCACAAATACCGACATATTCTACTTTAATCAAAACTTCAtcttc                                             <  2:2245271/146‑1 (MQ=255)

                                                                                                      ccACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCAtcttc                                             <  1:224806/148‑1 (MQ=255)

                                                                                                        acaGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAATCCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCACAACTTCATcttctt                                           >  2:681650/1‑148 (MQ=255)

                                                                                                          aGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTa                                         >  2:2951701/1‑148 (MQ=255)

                                                                                                           gCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAg                                        <  1:3045817/148‑1 (MQ=255)

                                                                                                            ccACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAAGTTCATCTTCTTTAgg                                       >  1:748299/1‑148 (MQ=255)

                                                                                                              acaACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCa                                     <  2:3508893/148‑1 (MQ=255)

                                                                                                              acaACCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGTTTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACCTCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCa                                     <  2:3746939/148‑1 (MQ=255)

                                                                                                                aaCCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACt                                   >  2:190677/1‑148 (MQ=255)

                                                                                                                aaCCGTCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACt                                   <  1:167030/148‑1 (MQ=255)

                                                                                                                 aCCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGTGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTg                                  <  2:1611006/148‑1 (MQ=255)

                                                                                                                 aCCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGTGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTg                                  <  2:901650/148‑1 (MQ=255)

                                                                                                                    gTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTGGTa                               <  1:1264776/148‑1 (MQ=255)

                                                                                                                     tCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTGGTAt                              >  1:564302/1‑148 (MQ=255)

                                                                                                                     tCCCGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTGGTAt                              <  1:2913627/148‑1 (MQ=255)

                                                                                                                      cccGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTGGTAtt                             >  1:2339378/1‑148 (MQ=255)

                                                                                                                       ccGGCGCATTCATGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTGGTAttt                            <  1:833898/148‑1 (MQ=255)

                                                                                                                                  aTGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTGGTATTTCTGCTGAaata                 <  1:1183177/148‑1 (MQ=255)

                                                                                                                                  aTGACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTGGTATTTCTGCTGAaata                 >  2:730372/1‑148 (MQ=255)

                                                                                                                                    gACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTGGTATTTCTGCTGAaataat               <  2:3567187/148‑1 (MQ=255)

                                                                                                                                    gACCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATATTCTTTAGGCACTGGTATTTCTGCTGAaataat               <  1:3448624/148‑1 (MQ=255)

                                                                                                                                     aCCCAGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTGGTATTTCTGCTGAAATAAtt              <  1:2962628/148‑1 (MQ=255)

                                                                                                                                         aGGCCAATTTATTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTGGTATTTCTGCTGAAATAATTTTCa          <  2:1207190/148‑1 (MQ=255)

                                                                                                                                           gCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTGGTATTTCTGCTGAAATAATTTTCAtt        >  2:3806651/1‑148 (MQ=255)

                                                                                                                                            ccAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTGGTATTTCTGCTGAAATAATTTTCATtg       <  2:2276205/148‑1 (MQ=255)

                                                                                                                                              aaTTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTGGTATTTCTGCTGAAATAATTTTCATtgtg     <  2:985729/148‑1 (MQ=255)

                                                                                                                                                 ttCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTGGTATTTCTGCTGAAATAATTTTCATTGTGccc  >  2:1259163/1‑148 (MQ=255)

                                                                                                                                                 ttCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTGGTATTTCTGCTGAAATAATTTTCATTGTGccc  >  2:1856307/1‑148 (MQ=255)

AGATGTTATATTTGCCTTCCAGACAGTAACGACAGTGACCGCAAGGAACGCCAGGTTCGATATTTACCCGATCCCCCGGTTTAAATTTGCGCACGCGGCTTCCCACAGCCACAACCGTCCCGGCGCATTCATGACCCAGGCCAATTTCTTGATTTGGGTCTTTAGGCGGAATAAACGGGCCTGATTCAAAACCATGTACATCTGAACCACAAATACCGACATATTCTACTTTAATCAAAACTTCATCTTCTTTAGGCACTGGTATTTCTGCTGAAATAATTTTCATTGTGCCC  >  CP009273/1852766‑1853058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A