Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F55 I0 R1 519 344.2 11360120 95.0% 10792114 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 461,641 G→A 36.8% V376V (GTC→GTT)  ybaE ← putative ABC superfamily transporter periplasmic‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273461,6410GA36.8% 406.9 / inf 365V376V (GTC→GTT) ybaEputative ABC superfamily transporter periplasmic‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (105/125);  new base A (76/58);  total (181/184)
Fisher's exact test for biased strand distribution p-value = 5.03e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 373 total aligned reads displayed.

TGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCAGCTC  >  CP009273/461492‑461790
                                                                                                                                                      |                                                                                                                                                     
tGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCg                                                                                                                                                       <  2:4730315/150‑1 (MQ=255)
  tAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGAc                                                                                                                                                     <  1:4175239/150‑1 (MQ=255)
   aGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACt                                                                                                                                                    >  2:3373844/1‑150 (MQ=255)
   aGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACt                                                                                                                                                    >  1:2573976/1‑150 (MQ=255)
   aGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACt                                                                                                                                                    >  1:782276/1‑150 (MQ=255)
     gCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTc                                                                                                                                                  <  2:3414806/150‑1 (MQ=255)
      cGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCt                                                                                                                                                 <  2:4583050/150‑1 (MQ=255)
      cGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCt                                                                                                                                                 <  1:237307/150‑1 (MQ=255)
         tCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAAc                                                                                                                                              >  1:3723745/1‑150 (MQ=255)
            gCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGtt                                                                                                                                           <  2:4183208/150‑1 (MQ=255)
             ccATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGttt                                                                                                                                          >  2:793731/1‑150 (MQ=255)
               aTGGTATGAAGTTCTATCGGTAGGTGATAACCCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGt                                                                                                                                        <  2:3700816/150‑1 (MQ=255)
               aTGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGt                                                                                                                                        <  1:882882/150‑1 (MQ=255)
               aTGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGt                                                                                                                                        <  2:2081890/150‑1 (MQ=255)
                tGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGta                                                                                                                                       <  1:5044642/150‑1 (MQ=255)
                tGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGta                                                                                                                                       <  1:4594070/150‑1 (MQ=255)
                   tATGAAGTTCTATCGGGAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGtaata                                                                                                                                    <  2:5489070/150‑1 (MQ=255)
                       aaGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAAcc                                                                                                                                >  2:1848992/1‑150 (MQ=255)
                           tCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGAt                                                                                                                            >  2:513609/1‑150 (MQ=255)
                             tATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATACACCGGAtt                                                                                                                           >  1:3827865/1‑150 (MQ=255)
                               tCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATtgat                                                                                                                        >  2:687366/1‑150 (MQ=255)
                               tCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATtgat                                                                                                                        <  1:1044176/150‑1 (MQ=255)
                                 gtgaGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATtgatga                                                                                                                      <  2:2971027/147‑1 (MQ=255)
                                  gTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGAGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGaa                                                                                                                     >  1:1659276/1‑150 (MQ=255)
                                   tAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGaat                                                                                                                    >  1:3077351/1‑150 (MQ=255)
                                   tAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGaat                                                                                                                    >  1:3930041/1‑150 (MQ=255)
                                    aGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATCAACCGGATTGATGaata                                                                                                                   >  2:4960155/1‑150 (MQ=255)
                                     tgTGATAAAACAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTTGCTGT‑AATGCATGACTTGCGGTTATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGaataa                                                                                                                  <  1:4844449/149‑1 (MQ=255)
                                     ggTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGaataa                                                                                                                  <  1:2084308/150‑1 (MQ=255)
                                       tGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATg                                                                                                                <  1:5434370/150‑1 (MQ=255)
                                        gATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATgg                                                                                                               <  1:2251569/150‑1 (MQ=255)
                                          tAAACCTGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGgag                                                                                                             <  2:4010312/150‑1 (MQ=255)
                                             aCCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATc                                                                                                          <  2:2156724/150‑1 (MQ=255)
                                             aCCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATc                                                                                                          <  2:2789251/150‑1 (MQ=255)
                                                  cGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACttt                                                                                                     <  1:3578641/150‑1 (MQ=255)
                                                   gTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATCATGGAGATCACtttt                                                                                                    >  2:2463360/1‑150 (MQ=255)
                                                   gTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACtttt                                                                                                    >  2:132934/1‑150 (MQ=255)
                                                   gTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACtttt                                                                                                    >  1:4342644/1‑150 (MQ=255)
                                                   gTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACATGCAAATGCGGAAAATTCCAGCCAGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCAGACTTCTAACTATTGTAATAAACATGATTGATGAATAATGGAGATAACtatt                                                                                                    >  2:5027197/1‑150 (MQ=255)
                                                    tCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTc                                                                                                   >  1:5274788/1‑150 (MQ=255)
                                                      aaGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTcgc                                                                                                 >  1:3523157/1‑150 (MQ=255)
                                                      aaGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTcgc                                                                                                 <  2:780997/150‑1 (MQ=255)
                                                       aGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTcgcg                                                                                                <  2:1255760/150‑1 (MQ=255)
                                                        ggTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCgcgc                                                                                               >  1:123274/1‑150 (MQ=255)
                                                              cGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCa                                                                                         >  1:5241261/1‑150 (MQ=255)
                                                               ggTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCag                                                                                        >  2:3125794/1‑150 (MQ=255)
                                                                  aGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAgg                                                                                     >  1:5611929/1‑150 (MQ=255)
                                                                   gTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAggg                                                                                    >  1:1908596/1‑150 (MQ=255)
                                                                       gACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGagag                                                                                >  1:1521660/1‑150 (MQ=255)
                                                                          ttCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGTAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCg                                                                             >  2:2926352/1‑150 (MQ=255)
                                                                           tCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCgg                                                                            <  2:2743293/150‑1 (MQ=255)
                                                                              tCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGa                                                                         <  1:3800106/150‑1 (MQ=255)
                                                                               ccGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGAc                                                                        <  1:4842961/150‑1 (MQ=255)
                                                                                     ccTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTgc                                                                  <  1:3373844/150‑1 (MQ=255)
                                                                                       tGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTgcgc                                                                >  1:3131167/1‑150 (MQ=255)
                                                                                       tGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTgcgc                                                                <  2:4097387/150‑1 (MQ=255)
                                                                                       tGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTgcgc                                                                >  1:2678468/1‑150 (MQ=255)
                                                                                          cAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCCGAGGGAGAGTCGGGGACTTTTGCGCAAc                                                             >  2:4940895/1‑150 (MQ=255)
                                                                                          cAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAAc                                                             >  1:952401/1‑150 (MQ=255)
                                                                                          cAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAAc                                                             <  2:3953874/150‑1 (MQ=255)
                                                                                          cAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAAc                                                             >  1:3797302/1‑150 (MQ=255)
                                                                                          cAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAAc                                                             >  2:612531/1‑150 (MQ=255)
                                                                                          cAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAAc                                                             >  2:3628776/1‑150 (MQ=255)
                                                                                           aaTGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACg                                                            >  1:3013516/1‑150 (MQ=255)
                                                                                            aTGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGt                                                           >  2:3926227/1‑150 (MQ=255)
                                                                                               cGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCaa                                                        <  2:2305070/150‑1 (MQ=255)
                                                                                               cGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCaa                                                        <  1:1345384/150‑1 (MQ=255)
                                                                                               cGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCaa                                                        >  1:3294042/1‑150 (MQ=255)
                                                                                               cGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCaa                                                        <  2:5606196/150‑1 (MQ=255)
                                                                                               cGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTCTTGCGCAACGTCaa                                                        <  2:1438416/150‑1 (MQ=255)
                                                                                                 gAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAAt                                                      <  1:4289470/150‑1 (MQ=255)
                                                                                                      gTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCACCGTCAAATAGCaa                                                 >  2:1567528/1‑150 (MQ=255)
                                                                                                       tCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCaaa                                                <  1:1006353/150‑1 (MQ=255)
                                                                                                         cAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGCCTTTTGCGCACCGTCCAATAGCaaaaa                                              >  2:5312711/1‑150 (MQ=255)
                                                                                                         cAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCaaaaa                                              >  2:2496356/1‑150 (MQ=255)
                                                                                                            ccTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCt                                           <  2:1872165/150‑1 (MQ=255)
                                                                                                             cTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTa                                          >  2:4683596/1‑150 (MQ=255)
                                                                                                              tGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTaa                                         >  2:2418507/1‑150 (MQ=255)
                                                                                                                       aTGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGc                                 <  1:1960136/150‑1 (MQ=255)
                                                                                                                       aTGCATGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGc                                 <  2:479040/150‑1 (MQ=255)
                                                                                                                        tGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGcc                                <  1:5524153/150‑1 (MQ=255)
                                                                                                                         gCATGACTGGCTGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCg                               <  1:3997865/150‑1 (MQ=255)
                                                                                                                           aTGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCt                             <  1:2785492/150‑1 (MQ=255)
                                                                                                                           aTGACTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCt                             <  2:5187756/150‑1 (MQ=255)
                                                                                                                               cTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTg                         >  2:924354/1‑150 (MQ=255)
                                                                                                                               cTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTg                         >  2:1075754/1‑150 (MQ=255)
                                                                                                                               cTGGCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGCACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTg                         <  2:1631763/150‑1 (MQ=255)
                                                                                                                                 ggCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGAc                       <  1:2051802/150‑1 (MQ=255)
                                                                                                                                  gCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGAcc                      <  1:1848992/150‑1 (MQ=255)
                                                                                                                                  gCGGTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGAcc                      >  2:592241/1‑150 (MQ=255)
                                                                                                                                    ggTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTg                    <  1:2279709/150‑1 (MQ=255)
                                                                                                                                    ggTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTg                    >  2:761973/1‑150 (MQ=255)
                                                                                                                                    ggTGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTg                    <  1:1456496/150‑1 (MQ=255)
                                                                                                                                      tGATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGc                  >  2:1150287/1‑150 (MQ=255)
                                                                                                                                       gATCAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTACGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCt                 >  2:2597858/1‑150 (MQ=255)
                                                                                                                                        aTCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTg                <  1:4523238/150‑1 (MQ=255)
                                                                                                                                          cAGGTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCACAAACCTAAACTGATGCCGCTACTGACCTGGCTGAc              >  1:916683/1‑150 (MQ=255)
                                                                                                                                            ggTTTTCTCCAACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTGAccc            >  1:2413676/1‑150 (MQ=255)
                                                                                                                                              ttttCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTGACCCGt          >  1:658942/1‑150 (MQ=255)
                                                                                                                                               tttCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTGACCCGtt         >  2:3787910/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGT‑AATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCAGCTC  >  CP009273/461492‑461790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A