Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,687,003 T→A 26.2% R36* (AGA→TGA)  uidC ← putative outer membrane porin for beta‑glucuronides porin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,687,0030TA26.2% 760.6 / 295.9 419R36* (AGA→TGA) uidCputative outer membrane porin for beta‑glucuronides porin protein
Reads supporting (aligned to +/- strand):  ref base T (158/150);  new base A (64/45);  total (223/196)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 420 total aligned reads displayed.

TAAACATAATACGCCCCGCCTTCAACGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAAT  >  CP009273/1686855‑1687151
                                                                                                                                                    |                                                                                                                                                    
     ataataCGCCCCGCCTTCAACGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCg                                                                                                                                                >  1:2411586/1‑150 (MQ=255)
       aataCGCCCCGCCTTCAACGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTa                                                                                                                                              >  2:1475862/1‑150 (MQ=255)
       aataCGCCCCGCCTTCAACGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTa                                                                                                                                              >  2:4376363/1‑150 (MQ=255)
       aataCGCCCCGCCTTCAACGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCAGATGCGTa                                                                                                                                              >  1:4853529/1‑150 (MQ=255)
        ataCGCCCCGCCTTCAACGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAg                                                                                                                                             >  2:4843981/1‑150 (MQ=255)
          aCGCCCCGCCTTCAACGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTc                                                                                                                                           <  1:2347983/150‑1 (MQ=255)
            gCCCCGCCTTCAACGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTccc                                                                                                                                         >  2:4563452/1‑150 (MQ=255)
            gCCCCGCCTTCAACGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTccc                                                                                                                                         <  1:4756026/150‑1 (MQ=255)
            gCCCCGCCTTCAACGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTccc                                                                                                                                         >  1:4426766/1‑150 (MQ=255)
            gCCCCGCCTTCAACGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCAGATGCGTAGTccc                                                                                                                                         >  2:4595827/1‑150 (MQ=255)
             ccccGCCTTCAACGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCGTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCg                                                                                                                                        <  1:3998771/150‑1 (MQ=255)
              cccGCCTTCAACGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGc                                                                                                                                       >  2:593709/1‑150 (MQ=255)
                    ttCAACGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCAAGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATc                                                                                                                                 <  2:5276587/150‑1 (MQ=255)
                       aaCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTc                                                                                                                              <  1:3180316/150‑1 (MQ=255)
                       aaCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTc                                                                                                                              >  1:5001159/1‑150 (MQ=255)
                            cAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCa                                                                                                                         >  1:4805364/1‑150 (MQ=255)
                             aataatATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAg                                                                                                                        >  1:2093132/1‑150 (MQ=255)
                               taataTTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGtt                                                                                                                      <  2:5073419/150‑1 (MQ=255)
                               taataTTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGtt                                                                                                                      >  2:2002799/1‑150 (MQ=255)
                                 atatTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTgc                                                                                                                    <  1:2846810/150‑1 (MQ=255)
                                  tatTGGAATAAAAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTgcg                                                                                                                   >  1:2737392/1‑150 (MQ=255)
                                      ggAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGcgccgc                                                                                                               >  2:4455231/1‑150 (MQ=255)
                                           aataaCCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAg                                                                                                          >  1:4039176/1‑150 (MQ=255)
                                           aataaCCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAg                                                                                                          >  1:741876/1‑150 (MQ=255)
                                            ataaCCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGc                                                                                                         <  2:4474400/150‑1 (MQ=255)
                                              aaCCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCag                                                                                                       >  2:3934068/1‑150 (MQ=255)
                                               aCCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCaga                                                                                                      <  2:1166540/150‑1 (MQ=255)
                                                ccACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCagag                                                                                                     >  2:2950317/1‑150 (MQ=255)
                                                 cACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAgg                                                                                                    <  1:1313135/150‑1 (MQ=255)
                                                   cTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTa                                                                                                  >  2:1752219/1‑150 (MQ=255)
                                                   cTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTa                                                                                                  >  1:5086716/1‑150 (MQ=255)
                                                     aTTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCACTTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAg                                                                                                >  2:4865331/1‑150 (MQ=255)
                                                     aTTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAg                                                                                                >  1:83940/1‑150 (MQ=255)
                                                     aTTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATATCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAg                                                                                                <  1:558119/150‑1 (MQ=255)
                                                      ttGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAgg                                                                                               <  1:3281993/150‑1 (MQ=255)
                                                       tGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGc                                                                                              >  1:4818201/1‑150 (MQ=255)
                                                       tGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGc                                                                                              >  1:4771747/1‑150 (MQ=255)
                                                        gAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGcc                                                                                             <  1:1810550/150‑1 (MQ=255)
                                                          aaTCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCag                                                                                           >  2:734146/1‑150 (MQ=255)
                                                          aaTCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCag                                                                                           <  1:5195722/150‑1 (MQ=255)
                                                          aaTCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCag                                                                                           <  1:2592872/150‑1 (MQ=255)
                                                                gCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAg                                                                                     >  2:1548034/1‑150 (MQ=255)
                                                                  aaTCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGcc                                                                                   >  2:2426163/1‑150 (MQ=255)
                                                                  aaTCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCCGAGGTAAGGCCAGAGGCAGcc                                                                                   >  2:3466410/1‑150 (MQ=255)
                                                                   aTCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCg                                                                                  >  1:2022337/1‑150 (MQ=255)
                                                                      ctccCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCa                                                                               <  1:4477302/150‑1 (MQ=255)
                                                                       tccCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAg                                                                              >  1:1558928/1‑150 (MQ=255)
                                                                       tccCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAg                                                                              <  1:3520138/150‑1 (MQ=255)
                                                                         ccTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGc                                                                            <  2:5001159/150‑1 (MQ=255)
                                                                          cTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCa                                                                           >  2:2153753/1‑150 (MQ=255)
                                                                          cTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCa                                                                           <  2:1622910/150‑1 (MQ=255)
                                                                              aCCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATa                                                                       <  1:1931719/150‑1 (MQ=255)
                                                                               cccATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATaa                                                                      <  1:1391254/150‑1 (MQ=255)
                                                                                ccATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAAc                                                                     <  2:3232950/150‑1 (MQ=255)
                                                                                ccATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAAc                                                                     <  1:4278327/150‑1 (MQ=255)
                                                                                ccATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAAc                                                                     >  2:664062/1‑150 (MQ=255)
                                                                                 cATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGCTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACg                                                                    <  2:87522/150‑1 (MQ=255)
                                                                                  aTGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACgg                                                                   >  2:1338974/1‑150 (MQ=255)
                                                                                        aaaTATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTCCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATgg                                                             >  1:3330114/1‑150 (MQ=255)
                                                                                         aaTATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGc                                                            >  2:2394039/1‑150 (MQ=255)
                                                                                           tatCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCa                                                          <  1:1727448/150‑1 (MQ=255)
                                                                                            atCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCAc                                                         <  2:3380142/150‑1 (MQ=255)
                                                                                             tctcTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACt                                                        >  1:1774532/1‑150 (MQ=255)
                                                                                               tctTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTAt                                                      >  2:5143744/1‑150 (MQ=255)
                                                                                               tctTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTAt                                                      >  2:3848907/1‑150 (MQ=255)
                                                                                               tcgtCCCGCGCCAGCTCTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCTTTATGATGCGTAGTCCCTCTTCATCGTCAGCGAGTTGCTCCGCATAAGCAGAGGTAAGGCAAGAGGAAGCCGTCAGGCAAATAACGGCCATGGCCACTAt                                                      <  2:787225/147‑1 (MQ=255)
                                                                                                 ttCCCGCGCCAGCAGTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCCGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCTGGAAAATAACGGCCATGGCCACTAttt                                                    <  1:3592491/150‑1 (MQ=255)
                                                                                                   cccGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTAttttt                                                  <  2:2495824/150‑1 (MQ=255)
                                                                                                       cgcCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCa                                              >  1:2503714/1‑150 (MQ=255)
                                                                                                        gcCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCAt                                             >  1:1076462/1‑150 (MQ=255)
                                                                                                        gcCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCAt                                             >  2:3685948/1‑150 (MQ=255)
                                                                                                             cACTGGGCTTATCCGCCCTGCGCAATTCGTTTGTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTg                                        >  2:2634353/1‑150 (MQ=255)
                                                                                                               cTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCAGATGTGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCa                                      >  2:3080205/1‑150 (MQ=255)
                                                                                                                   gCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCtt                                  <  1:1676603/150‑1 (MQ=255)
                                                                                                                   gCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCtt                                  <  2:4233886/150‑1 (MQ=255)
                                                                                                                   gCTTAT‑CGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCttt                                 <  2:1301007/150‑1 (MQ=255)
                                                                                                                    cTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCttt                                 <  2:2259124/150‑1 (MQ=255)
                                                                                                                    cTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCtgt                                 >  2:4274725/1‑148 (MQ=255)
                                                                                                                     ttATCCGCCCTGCGCAATTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTg                                >  1:1469421/1‑150 (MQ=255)
                                                                                                                       aTCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGAt                              >  1:5247504/1‑150 (MQ=255)
                                                                                                                        tCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATg                             <  1:115996/150‑1 (MQ=255)
                                                                                                                            cccTGCGCAATTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGAAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCt                         <  1:1858297/150‑1 (MQ=255)
                                                                                                                                 cgcAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGatta                    <  1:3389794/150‑1 (MQ=255)
                                                                                                                                 cgcAATTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCACATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTGTGATGTTCTGatta                    >  1:3897242/1‑150 (MQ=255)
                                                                                                                                    aaTTCGTTTTTCTGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGAGGTTCTGATTAttt                 >  1:2962404/1‑150 (MQ=255)
                                                                                                                                    aaTTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCCGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTAttt                 >  2:4977546/1‑150 (MQ=255)
                                                                                                                                    aaTTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTGTGATGTTCTGATTAttt                 >  1:3083574/1‑150 (MQ=255)
                                                                                                                                     aTTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCGTTGATGTTCTGATTAtttt                >  2:2062102/1‑150 (MQ=255)
                                                                                                                                     aTTCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTAtttt                <  1:2443308/150‑1 (MQ=255)
                                                                                                                                      ttCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCCGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTAttttt               <  2:4087203/150‑1 (MQ=255)
                                                                                                                                      ttCGTTTTTCAGTCAGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTAttttt               <  2:4741572/150‑1 (MQ=255)
                                                                                                                                       tCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTa              <  1:4563452/150‑1 (MQ=255)
                                                                                                                                         gTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTAtt            >  1:2478093/1‑150 (MQ=255)
                                                                                                                                          tttttCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTg           <  2:1207346/150‑1 (MQ=255)
                                                                                                                                            tttCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGaa         >  2:101268/1‑150 (MQ=255)
                                                                                                                                                aGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATatt     >  1:3696472/1‑150 (MQ=255)
                                                                                                                                                aGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATatt     >  1:4203144/1‑150 (MQ=255)
                                                                                                                                                 gTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATatta    >  1:2200943/1‑150 (MQ=255)
                                                                                                                                                   cTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATattaat  <  2:2752946/150‑1 (MQ=255)
                                                                                                                                                   cTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCACATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATattaat  >  1:935148/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TAAACATAATACGCCCCGCCTTCAACGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAAT  >  CP009273/1686855‑1687151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A