Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,836,192 T→A 100% I67I (ATA→ATT)  ynjH ← DUF1496 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,836,1920TA100.0% 888.7 / ‑6.7 272I67I (ATA→ATT) ynjHDUF1496 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (124/146);  total (124/148)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 272 total aligned reads displayed.

CGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTT  >  CP009273/1836045‑1836338
                                                                                                                                                    |                                                                                                                                                  
cgcTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAAt                                                                                                                                                  <  2:852500/150‑1 (MQ=255)
 gcTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAAtt                                                                                                                                                 <  1:4660491/150‑1 (MQ=255)
 gcTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAAtt                                                                                                                                                 <  2:2654522/150‑1 (MQ=255)
 gcTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAAtt                                                                                                                                                 >  2:3899780/1‑150 (MQ=255)
   tggtctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTcc                                                                                                                                               <  2:4225174/150‑1 (MQ=255)
    ggtctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCt                                                                                                                                              <  2:1007108/150‑1 (MQ=255)
    ggtctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCAATGCAGCAGAATTCCt                                                                                                                                              >  1:2202293/1‑150 (MQ=255)
        tggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATTGGGTTTA‑GCGCCGCCACACCAGCGGATTATTGCTAAGGGGTTTTTCATAACGCTGGCCTTGCAGCAGAAttacatagg                                                                                                                                          >  1:1900387/1‑143 (MQ=255)
        tggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCgg                                                                                                                                          <  1:1083175/150‑1 (MQ=255)
          gtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCt                                                                                                                                        <  1:4772976/150‑1 (MQ=255)
          gtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGGTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCt                                                                                                                                        >  2:2768995/1‑150 (MQ=255)
             cTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTa                                                                                                                                     <  1:4998282/150‑1 (MQ=255)
              tCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTaa                                                                                                                                    <  1:1223460/150‑1 (MQ=255)
                 ccTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTAAGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAAATCCGTCGGCGTTAATTa                                                                                                                                 >  1:2888689/1‑150 (MQ=255)
                   tGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTAcc                                                                                                                               <  2:1881859/150‑1 (MQ=255)
                      agagGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGcc                                                                                                                            <  2:2469243/150‑1 (MQ=255)
                       gagGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGccc                                                                                                                           <  1:377907/150‑1 (MQ=255)
                          gcgcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCtt                                                                                                                        <  2:2623592/150‑1 (MQ=255)
                           cgcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTc                                                                                                                       <  2:3346499/150‑1 (MQ=255)
                             cTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAg                                                                                                                     <  2:746836/150‑1 (MQ=255)
                              tGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGa                                                                                                                    >  2:1256299/1‑150 (MQ=255)
                               tcAATATACTCTGGCCCCTGCTGACAGTCCATTATTAGAGGCGTTTA‑TGTCTTTACGCTCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGaa                                                                                                                   <  2:4497992/149‑1 (MQ=255)
                                cAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGaat                                                                                                                  <  2:914263/150‑1 (MQ=255)
                                   tatCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGaataat                                                                                                               <  2:5287365/150‑1 (MQ=255)
                                   tatCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCAAGCATTGCAGCAGAATTCCATAGGCTTTAAATAACGCCCCAACAGaaaaaa                                                                                                               >  1:4662068/1‑149 (MQ=255)
                                      ccGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAAtttt                                                                                                            <  1:2349673/150‑1 (MQ=255)
                                         cTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGAt                                                                                                         <  2:2604575/150‑1 (MQ=255)
                                         cTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGAt                                                                                                         <  2:3691797/150‑1 (MQ=255)
                                            ggcccTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCt                                                                                                      <  1:4359948/148‑1 (MQ=255)
                                                ctgctgACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATa                                                                                                  <  2:3984401/150‑1 (MQ=255)
                                                ctgctgACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCAATGCAGCAGAATTCCTTCGGCTTTAATTAACGCCCCATCAGAATAATTTTGATCCTGATa                                                                                                  >  2:5173503/1‑150 (MQ=255)
                                                  gctgACATTCCATTATTAGAGGCGTTTA‑TGGCTTTAAGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTTTCATCACGCTGGCATTTCAGCAGAAATACATAGGCTTTAATTAACGCCCCAACAGAAAAATTTTTATCCTGATaaa                                                                                                >  1:4255807/1‑150 (MQ=255)
                                                    tgACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACg                                                                                              >  2:2083220/1‑150 (MQ=255)
                                                    tgACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACg                                                                                              <  2:1514098/150‑1 (MQ=255)
                                                       cATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgcag                                                                                           >  2:1173397/1‑150 (MQ=255)
                                                       cATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAAGTTTGATCCTGATAAACgcag                                                                                           >  2:3843001/1‑150 (MQ=255)
                                                       cATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCAAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgcgg                                                                                           >  2:1319068/1‑148 (MQ=255)
                                                          tCCATTATTAGAGGCGTTTA‑TTGCTTTAAGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCAAAACGCTATCAATGCAGCAGAAATCCATCGGCTTTAATTACCGCCCCTTCAGAATAAATTTGATACAGATAAAAGcaccac                                                                                        >  2:3572430/1‑150 (MQ=255)
                                                          tCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCAc                                                                                        >  2:3752076/1‑150 (MQ=255)
                                                           ccATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACt                                                                                       <  2:4684396/150‑1 (MQ=255)
                                                            cATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCAATGCAGCAGAATTACTTCGGCATTAATTAACGCCCCATCAGAATAAATTTGATACAGATAAACGCAGCACTg                                                                                      >  2:2419960/1‑150 (MQ=255)
                                                             attattAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGa                                                                                     <  1:1159484/150‑1 (MQ=255)
                                                                attaGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACAAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTa                                                                                  >  2:4775612/1‑150 (MQ=255)
                                                                attaGAGGCGTTTA‑TGGCTTTAAGCGCCGCCAGACCAGCGGATTATTGCTAAGGGTTTTGTCAACACGCAGGCATTGCAGCAGAAATCCATCGGCTTTAATTACCCCCCCATCAGAAAAATTTTTATCCTGATAAACGCAGCACAGAGTa                                                                                  >  1:1295707/1‑150 (MQ=255)
                                                                 ttaGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGttc                                                                                 <  1:72222/150‑3 (MQ=255)
                                                                  taGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACa                                                                                <  2:2911834/150‑1 (MQ=255)
                                                                    gagGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAgg                                                                              <  1:3456691/150‑1 (MQ=255)
                                                                        cGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTg                                                                          >  2:1879605/1‑150 (MQ=255)
                                                                         gTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAGGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTgc                                                                         >  2:1563235/1‑150 (MQ=255)
                                                                             a‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCGGCACTGAGTACAGGGCTGCGCTg                                                                     >  2:309166/1‑150 (MQ=255)
                                                                             a‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTg                                                                     <  1:1424616/150‑1 (MQ=255)
                                                                               tGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGa                                                                    >  2:2276685/1‑150 (MQ=255)
                                                                                 gCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAATGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCTCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGAACTGAGTACAGGGCTGCGCTGACt                                                                  >  2:2528570/1‑150 (MQ=255)
                                                                                 gCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACt                                                                  >  1:3016064/1‑150 (MQ=255)
                                                                                  cTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTg                                                                 >  1:3510937/1‑150 (MQ=255)
                                                                                   tttACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGc                                                                >  1:4747710/1‑150 (MQ=255)
                                                                                     tACGCGCCGCCAGACCAGCGGATTAGTGCTATGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGccc                                                              <  2:4992634/150‑1 (MQ=255)
                                                                                     tACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGccc                                                              >  1:4918781/1‑150 (MQ=255)
                                                                                     tACGCGCAGCCAGACAAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGAATTGCATCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGccc                                                              <  1:3312095/150‑1 (MQ=255)
                                                                                                  aCCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCtgaactgaac                                                 >  2:728368/1‑150 (MQ=255)
                                                                                                   ccAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAaca                                                <  2:3442794/150‑1 (MQ=255)
                                                                                                     aGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACt                                              >  1:1029606/1‑150 (MQ=255)
                                                                                                     aGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACt                                              >  2:593308/1‑150 (MQ=255)
                                                                                                     aGCGGATTAGAGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCATCAGAATAATTTTGATCCAGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACt                                              >  1:4072957/1‑150 (MQ=255)
                                                                                                        ggATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCACCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGAACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCt                                           >  1:1850079/1‑150 (MQ=255)
                                                                                                         gATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTg                                          <  2:2017745/150‑1 (MQ=255)
                                                                                                         gATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTg                                          <  1:4888739/150‑1 (MQ=255)
                                                                                                           ttAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGc                                        <  1:3899728/150‑1 (MQ=255)
                                                                                                             aGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCgg                                      <  1:4688001/150‑1 (MQ=255)
                                                                                                             aGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCgg                                      <  2:5045467/150‑1 (MQ=255)
                                                                                                               tGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTa                                    >  2:1675091/1‑150 (MQ=255)
                                                                                                                 cTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACg                                  >  1:2667848/1‑150 (MQ=255)
                                                                                                                    aGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGttt                               >  1:249711/1‑150 (MQ=255)
                                                                                                                       gTTTTGTAATCACGCTGGCATTGCATCATAATTCCTTCGGCTTTAATTACCGCCCCTTCATAATAATTTTGATCCTGATAAACGCAGCACTTAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTAcc                            <  1:208728/150‑1 (MQ=255)
                                                                                                                        ttttGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCt                           >  1:5046641/1‑150 (MQ=255)
                                                                                                                         tttGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTc                          >  2:459706/1‑150 (MQ=255)
                                                                                                                          ttGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTcc                         >  1:1972187/1‑150 (MQ=255)
                                                                                                                          ttGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTcc                         <  2:4329607/150‑1 (MQ=255)
                                                                                                                          ttGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTcc                         <  2:4650423/150‑1 (MQ=255)
                                                                                                                          ttGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTcc                         <  1:2984571/150‑1 (MQ=255)
                                                                                                                           tGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCa                        >  2:518058/1‑150 (MQ=255)
                                                                                                                           tGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCa                        >  2:1797216/1‑150 (MQ=255)
                                                                                                                            gTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCAc                       <  2:4658085/150‑1 (MQ=255)
                                                                                                                            gTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATGACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCAc                       <  2:195455/150‑1 (MQ=255)
                                                                                                                             tcatcaCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCCGACTAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTGTACCTCCCCg                      >  2:2963786/1‑150 (MQ=255)
                                                                                                                              catcaCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGt                     >  2:4444135/1‑150 (MQ=255)
                                                                                                                              catcaCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGt                     >  2:439604/1‑150 (MQ=255)
                                                                                                                                  aCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCgg                 >  1:3676048/1‑150 (MQ=255)
                                                                                                                                     cTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACg              >  1:2849509/1‑150 (MQ=255)
                                                                                                                                      tagCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCATAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGa             <  1:5173503/148‑1 (MQ=255)
                                                                                                                                       ggCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGAt            <  1:821203/150‑1 (MQ=255)
                                                                                                                                         cATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATaa          >  1:3863361/1‑150 (MQ=255)
                                                                                                                                          aTTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAAt         <  2:3164293/150‑1 (MQ=255)
                                                                                                                                          aTTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAAt         <  1:1215855/150‑1 (MQ=255)
                                                                                                                                           ttGCAGCATAATTCCTTCGTATTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCCCTGAGTAAATGGCCGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATg        <  1:1734229/150‑1 (MQ=255)
                                                                                                                                           ttGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATg        <  1:323907/150‑1 (MQ=255)
                                                                                                                                               agcagAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCggg    >  2:4019618/1‑150 (MQ=255)
                                                                                                                                               agcagAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCggg    >  2:1073851/1‑150 (MQ=255)
                                                                                                                                               agcagAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCggg    >  2:2088055/1‑150 (MQ=255)
                                                                                                                                                 cagAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGtt  >  1:1537466/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTA‑TGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTT  >  CP009273/1836045‑1836338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGC  >  CP009273/1836044‑1836341
                                                                                                                                                    |                                                                                                                                                     
GCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAA                                                                                                                                                      <  SRR19973062.1695575/150‑1 (MQ=60)
  GCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATT                                                                                                                                                    <  SRR19973062.4684870/150‑1 (MQ=60)
     GGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCT                                                                                                                                                 <  SRR19973062.3279053/150‑1 (MQ=60)
     GGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCAATGCAGCAGAATTCCT                                                                                                                                                 >  SRR19973062.2213336/1‑150 (MQ=60)
     GGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCACTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCT                                                                                                                                                 <  SRR19973062.1815288/150‑1 (MQ=60)
       TCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTC                                                                                                                                               <  SRR19973062.5342234/150‑1 (MQ=60)
        CTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCG                                                                                                                                              <  SRR19973062.3669507/150‑1 (MQ=60)
        CTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCG                                                                                                                                              <  SRR19973062.4879273/150‑1 (MQ=60)
         TGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGG                                                                                                                                             <  SRR19973062.1088278/150‑1 (MQ=60)
           GTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCT                                                                                                                                           <  SRR19973062.4797775/150‑1 (MQ=60)
              CTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTTTTGCTTTAAGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTTTAATCACGCTAGCATTGCAGCAGAAATGCTTCGGGTTTA                                                                                                                                        >  SRR19973062.1472890/1‑150 (MQ=60)
              CTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTA                                                                                                                                        <  SRR19973062.5024701/150‑1 (MQ=60)
               TCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAA                                                                                                                                       <  SRR19973062.1229146/150‑1 (MQ=60)
                CACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAAT                                                                                                                                      <  SRR19973062.4759372/150‑1 (MQ=60)
                  CCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTAAGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAAATCCGTCGGCGTTAATTA                                                                                                                                    >  SRR19973062.2903937/1‑150 (MQ=60)
                   CTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTAC                                                                                                                                   <  SRR19973062.3880263/150‑1 (MQ=60)
                       AGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCGTTAATTACCGCC                                                                                                                               >  SRR19973062.3071585/1‑150 (MQ=60)
                        GAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCC                                                                                                                              <  SRR19973062.2051936/150‑1 (MQ=60)
                        GAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCC                                                                                                                              <  SRR19973062.379597/150‑1 (MQ=60)
                            CGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGAGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTC                                                                                                                          <  SRR19973062.1540864/150‑1 (MQ=60)
                              CTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATTGGTTTAAGCGCCCCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTAGGCTTTAATTACCGCCCCATCAG                                                                                                                        >  SRR19973062.5071212/1‑150 (MQ=60)
                                GCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCATCAGAA                                                                                                                      >  SRR19973062.4362587/1‑150 (MQ=60)
                                GCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCGTTAATTACCGCCCCTTCAGAA                                                                                                                      >  SRR19973062.4362710/1‑150 (MQ=60)
                                    TATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAAT                                                                                                                  <  SRR19973062.109472/150‑1 (MQ=60)
                                    TATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCAAGCATTGCAGCAGAATTCCATAGGCTTTAAataacgccccaacagaaaaaa                                                                                                                  >  SRR19973062.4686455/1‑129 (MQ=60)
                                    TATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTGTATGGCTTTACGCGCCGCCACACCACCGGATTAGTGCTAAGGGTTTTGTCATCACGCAAGCATTTCAGCAGAATTACATAGGCTTTAATTAACGCCCCATCAGAAaaaa                                                                                                                  >  SRR19973062.4686176/1‑146 (MQ=60)
                                       CCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTT                                                                                                               <  SRR19973062.2362184/150‑1 (MQ=60)
                                       CCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTT                                                                                                               <  SRR19973062.1362930/150‑1 (MQ=60)
                                           TGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATC                                                                                                           <  SRR19973062.4709888/150‑1 (MQ=60)
                                           TGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATC                                                                                                           <  SRR19973062.4608143/150‑1 (MQ=60)
                                             GGCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCT                                                                                                         <  SRR19973062.4382339/150‑1 (MQ=60)
                                             GCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCT                                                                                                         <  SRR19973062.4382539/150‑1 (MQ=60)
                                             GCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCT                                                                                                         <  SRR19973062.4955402/150‑1 (MQ=60)
                                              CCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTG                                                                                                        <  SRR19973062.1162980/150‑1 (MQ=60)
                                                CCTGCTGACATTCCATTATTAGAGGCGTTTATTGCATTAAGCGCCGCCACACCAACGGATTAGTTCTAAGGGTTTTTTCATAAAGCTAGCAATTCAGCAGAATTACTTCGGCGTTAATTAACGCCCCAACAGAATAAATTTTTTCCTGAT                                                                                                      >  SRR19973062.2355181/1‑150 (MQ=33)
                                                CCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGAT                                                                                                      <  SRR19973062.1211482/150‑1 (MQ=60)
                                                CCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGAT                                                                                                      <  SRR19973062.2213430/150‑1 (MQ=60)
                                                CCTGCTGACATTCCATTATTAGAGGCGTGTTTTGCTTTACGCGCCGCCACACCCCCGGATTAGTGCTAAGGGTTTTGTCATAACGCTAGCAATGCAGCAGAATTACATCGGCGTTAATTACCGCCCCATCAGAATAAATTTGATCCAGAT                                                                                                      >  SRR19973062.1951606/1‑150 (MQ=60)
                                                  TGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAA                                                                                                    <  SRR19973062.3520355/150‑1 (MQ=60)
                                                   GCTGACATTCCATTATTAGAGGCGTTTATGGCTTTAAGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTTTCATCACGCTGGCATTTCAGCAGAAATACATAGGCTTTAATTAACGCCCCAACAGAAAAATTTTTATCCTGATAAA                                                                                                   >  SRR19973062.4277849/1‑150 (MQ=60)
                                                    CTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAAC                                                                                                  <  SRR19973062.2363981/150‑1 (MQ=60)
                                                       ACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCA                                                                                               <  SRR19973062.3430368/150‑1 (MQ=60)
                                                            CCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACT                                                                                          <  SRR19973062.3727196/150‑1 (MQ=60)
                                                              ATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGA                                                                                        <  SRR19973062.1164917/150‑1 (MQ=60)
                                                                 ATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTA                                                                                     <  SRR19973062.186670/150‑1 (MQ=60)
                                                                 ATTAGAGGCGTTTATGGCTTTAAGCGCCGCCAGACCAGCGGATTATTGCTAAGGGTTTTGTCAACACGCAGGCATTGCAGCAGAAATCCATCGGCTTTAATTACCCCCCCATCAGAAAAATTTTTATCCTGATAAACGCAGCACAGAGTA                                                                                     >  SRR19973062.1301639/1‑150 (MQ=60)
                                                                  TTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTTC                                                                                    <  SRR19973062.72712/150‑1 (MQ=60)
                                                                     GAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGG                                                                                 <  SRR19973062.3474313/150‑1 (MQ=60)
                                                                      AGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGG                                                                                <  SRR19973062.484355/150‑1 (MQ=60)
                                                                          GTTTATGGCTTTACGCGCCGCCAGACCAGAGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGC                                                                            >  SRR19973062.4744518/1‑150 (MQ=60)
                                                                           TTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCG                                                                           >  SRR19973062.2212459/1‑150 (MQ=60)
                                                                           TTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCG                                                                           <  SRR19973062.5110267/150‑1 (MQ=60)
                                                                           TTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCG                                                                           <  SRR19973062.5110179/150‑1 (MQ=60)
                                                                             TATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCCTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCT                                                                         <  SRR19973062.3903228/150‑1 (MQ=60)
                                                                             TATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCCTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCT                                                                         <  SRR19973062.3903217/150‑1 (MQ=60)
                                                                             TATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCT                                                                         <  SRR19973062.834693/150‑1 (MQ=60)
                                                                              ATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTG                                                                        <  SRR19973062.1431499/150‑1 (MQ=60)
                                                                                 GCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACT                                                                     <  SRR19973062.198433/150‑1 (MQ=60)
                                                                                 GCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACT                                                                     >  SRR19973062.3031783/1‑150 (MQ=60)
                                                                                 GCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACT                                                                     >  SRR19973062.3031819/1‑150 (MQ=60)
                                                                                  CTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTG                                                                    >  SRR19973062.183652/1‑150 (MQ=60)
                                                                                  CTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTG                                                                    >  SRR19973062.3528735/1‑150 (MQ=60)
                                                                                   TTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGC                                                                   >  SRR19973062.4772432/1‑150 (MQ=60)
                                                                                    TTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCATCAGAATAATTTTGATCCTGATAAACGAAGAACTGAGTACAGGGCTGCGCTGACTGCC                                                                  >  SRR19973062.1308489/1‑150 (MQ=60)
                                                                                     TACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCC                                                                 >  SRR19973062.4944344/1‑150 (MQ=60)
                                                                                     TACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCC                                                                 >  SRR19973062.3424132/1‑150 (MQ=60)
                                                                                     TACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCC                                                                 >  SRR19973062.4944334/1‑150 (MQ=60)
                                                                                     TACGCGCAGCCAGACAAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGAATTGCATCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCC                                                                 <  SRR19973062.3329222/150‑1 (MQ=60)
                                                                                         CGCCGCCAGACCTGCGTATTAGTGCTAAGGGTTTTGTCATCACGCTGGAATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCATTCATAATAATTTTGATCCTTATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCT                                                             <  SRR19973062.4121162/150‑1 (MQ=60)
                                                                                         CGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCT                                                             <  SRR19973062.3518733/150‑1 (MQ=60)
                                                                                                AGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGA                                                      >  SRR19973062.498194/1‑150 (MQ=60)
                                                                                                  ACCCGCGGATTAGTGCTATGGTTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTTATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAAC                                                    <  SRR19973062.3644999/150‑1 (MQ=60)
                                                                                                    CAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACAC                                                  >  SRR19973062.273387/1‑150 (MQ=60)
                                                                                                     AGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACT                                                 >  SRR19973062.1034550/1‑150 (MQ=60)
                                                                                                     AGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACT                                                 >  SRR19973062.1034432/1‑150 (MQ=60)
                                                                                                     AGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGATTTAATTACCGCCCCTTCAGAATAATTTTGATACTGATAAACGCAGCACTGAGTACAGTGCTGCGCTGACTGCCCGCCTGAACTGAACACT                                                 >  SRR19973062.4093880/1‑150 (MQ=60)
                                                                                                     AGCGGATTAGAGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCATCAGAATAATTTTGATCCAGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACT                                                 >  SRR19973062.4093874/1‑150 (MQ=60)
                                                                                                        GGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCT                                              >  SRR19973062.2137103/1‑150 (MQ=60)
                                                                                                        GGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCT                                              >  SRR19973062.2872833/1‑150 (MQ=60)
                                                                                                        GGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCT                                              >  SRR19973062.1297093/1‑150 (MQ=60)
                                                                                                        GGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCT                                              >  SRR19973062.2975548/1‑150 (MQ=60)
                                                                                                        GGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCACCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGAACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCT                                              >  SRR19973062.1859328/1‑150 (MQ=60)
                                                                                                         GATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACTGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTG                                             <  SRR19973062.4913952/150‑1 (MQ=60)
                                                                                                         GATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTG                                             <  SRR19973062.4913905/150‑1 (MQ=60)
                                                                                                         GATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTG                                             <  SRR19973062.818625/150‑1 (MQ=60)
                                                                                                           TTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGC                                           <  SRR19973062.3919642/150‑1 (MQ=60)
                                                                                                           TTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGC                                           <  SRR19973062.3919590/150‑1 (MQ=60)
                                                                                                             AGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGG                                         <  SRR19973062.4712506/150‑1 (MQ=60)
                                                                                                                 CTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACG                                     >  SRR19973062.2681843/1‑150 (MQ=60)
                                                                                                                    AGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTT                                  >  SRR19973062.250971/1‑150 (MQ=60)
                                                                                                                       GTTTTGTAATCACGCTGGCATTGCATCATAATTCCTTCGGCTTTAATTACCGCCCCTTCATAATAATTTTGATCCTGATAAACGCAGCACTTAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACC                               <  SRR19973062.209836/150‑1 (MQ=60)
                                                                                                                        TTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCT                              >  SRR19973062.190914/1‑150 (MQ=60)
                                                                                                                        TTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCT                              >  SRR19973062.5073396/1‑150 (MQ=60)
                                                                                                                          TTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCC                            >  SRR19973062.1982323/1‑150 (MQ=60)
                                                                                                                          TTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCC                            <  SRR19973062.3000221/150‑1 (MQ=60)
                                                                                                                             TCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACG                         <  SRR19973062.3110842/150‑1 (MQ=60)
                                                                                                                               ATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTAAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTC                       <  SRR19973062.3853671/150‑1 (MQ=60)
                                                                                                                                TCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCC                      >  SRR19973062.2822500/1‑150 (MQ=60)
                                                                                                                                  ACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGG                    >  SRR19973062.3694892/1‑150 (MQ=60)
                                                                                                                                   CGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGA                   >  SRR19973062.2556216/1‑150 (MQ=60)
                                                                                                                                    GCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGAC                  >  SRR19973062.2658377/1‑150 (MQ=60)
                                                                                                                                    GCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGAC                  <  SRR19973062.4784457/150‑1 (MQ=60)
                                                                                                                                     CTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACG                 >  SRR19973062.2864570/1‑150 (MQ=60)
                                                                                                                                      tagccTTGCAGCAGATTTCCTTCGGCTTTAATTACCGCCCCTTCTTAATAATTTTGTTCCTGTTACTCGCTGCCTTGTGTTCTGGGGTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCTCGTCCGGACGA                <  SRR19973062.5200899/145‑1 (MQ=60)
                                                                                                                                      TGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGA                >  SRR19973062.2956771/1‑150 (MQ=60)
                                                                                                                                      TAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCATAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGA                <  SRR19973062.5200826/150‑1 (MQ=60)
                                                                                                                                       GGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGAT               <  SRR19973062.824820/150‑1 (MQ=60)
                                                                                                                                       AGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTCCCTCCACGTCCGGACGAG               >  SRR19973062.895302/1‑150 (MQ=60)
                                                                                                                                         CATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAA             >  SRR19973062.3883080/1‑150 (MQ=60)
                                                                                                                                          ATTGCATCATAATTCCTTCGGCTTTAATTACCGCACATTCAGAATAATTTTGATCATTATAAACGCTGAACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAAT            <  SRR19973062.1221440/150‑1 (MQ=60)
                                                                                                                                          ATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAAT            <  SRR19973062.1221511/150‑1 (MQ=60)
                                                                                                                                          ATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAAT            >  SRR19973062.469914/1‑150 (MQ=60)
                                                                                                                                           TTGCAGCATAATTCCTTCGTATTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCCCTGAGTAAATGGCCGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATG           <  SRR19973062.1742671/150‑1 (MQ=60)
                                                                                                                                           TTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATG           <  SRR19973062.325412/150‑1 (MQ=60)
                                                                                                                                            TACAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACCGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGC          <  SRR19973062.1225944/150‑1 (MQ=60)
                                                                                                                                               AGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGG       >  SRR19973062.1710296/1‑150 (MQ=60)
                                                                                                                                                 CAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTT     >  SRR19973062.1545046/1‑150 (MQ=60)
                                                                                                                                                 CAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTT     <  SRR19973062.1262098/150‑1 (MQ=60)
                                                                                                                                                 CAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTT     >  SRR19973062.3023583/1‑150 (MQ=60)
                                                                                                                                                  AGAATTCCTTCTGATTTAATTACCGCCCATTCATAATAATTTTGATCCTGATAAACGCATCAATTTTTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTA    <  SRR19973062.1509484/150‑1 (MQ=60)
                                                                                                                                                    AATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGC  >  SRR19973062.3475749/1‑150 (MQ=60)
                                                                                                                                                    AATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGC  >  SRR19973062.3475785/1‑150 (MQ=60)
                                                                                                                                                    AATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGC  >  SRR19973062.2934307/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGC  >  CP009273/1836044‑1836341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑