Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,855,239 A→T 61.9% N117K (AAT→AAA)  ydjL ← putative Zn‑dependent NAD(P)‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,855,2390AT61.9% 232.8 / 285.9 264N117K (AAT→AAA) ydjLputative Zn‑dependent NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (55/45);  new base T (85/77);  total (142/122)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 279 total aligned reads displayed.

CGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCT  >  CP009273/1855091‑1855384
                                                                                                                                                    |                                                                                                                                                 
cgATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGtat                                                                                                                                                  >  1:4866233/1‑150 (MQ=255)
 gATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGtttt                                                                                                                                                 <  2:202333/150‑1 (MQ=255)
  aTGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGtttta                                                                                                                                                >  2:190822/1‑150 (MQ=255)
   tGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCACCACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGtattat                                                                                                                                               <  1:1050842/150‑1 (MQ=255)
   tGGATTTGTAGGAATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCAGGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGtattat                                                                                                                                               <  1:4990116/150‑1 (MQ=255)
    ggATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCAACAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGttttatt                                                                                                                                              <  2:2121736/150‑1 (MQ=255)
      acttGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGtattattat                                                                                                                                            <  1:5093010/148‑1 (MQ=255)
      aTTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGttttattat                                                                                                                                            >  2:1378093/1‑150 (MQ=255)
      aTTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGtattattat                                                                                                                                            <  2:2860847/150‑1 (MQ=255)
       tttGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATc                                                                                                                                           <  1:3986173/150‑1 (MQ=255)
           tAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGa                                                                                                                                       <  1:2558713/150‑1 (MQ=255)
             ggCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGAcc                                                                                                                                     >  1:1322014/1‑150 (MQ=255)
               cATGACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACACTATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCaa                                                                                                                                   >  1:4793191/1‑150 (MQ=255)
                aTTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCATGAACCAGACAATATTTGGAAAAACCACAGCCCCAGGTTTTATTATCCAGACCAAg                                                                                                                                  >  1:2347185/1‑150 (MQ=255)
                aTTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAg                                                                                                                                  >  1:5168011/1‑150 (MQ=255)
                aTTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAg                                                                                                                                  >  1:4328782/1‑150 (MQ=255)
                aTTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAAACAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTATGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAg                                                                                                                                  >  2:1334743/1‑150 (MQ=255)
                 ttACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAgg                                                                                                                                 <  1:2290055/150‑1 (MQ=255)
                   aCAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGtt                                                                                                                               >  1:3282762/1‑150 (MQ=255)
                    cAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGttt                                                                                                                              >  1:1367497/1‑150 (MQ=255)
                     aGATAGGGTCAAGTACGTCTGCGTCAACAAAATCAACAAAATCAGGTAATTACCACAACGCATTACGAAGAATTTTTAGAATTTCACAAGGAACCAGACAATATTTGGAAAAACCCACGCACCATGATTTATAAACCAGAACAAGATTTa                                                                                                                             >  1:381139/1‑150 (MQ=255)
                         aGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACttt                                                                                                                         <  2:593191/150‑1 (MQ=255)
                          gggTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACtttt                                                                                                                        <  2:2918301/150‑1 (MQ=255)
                             tCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCt                                                                                                                     <  2:4188256/150‑1 (MQ=255)
                             tCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGAATTATTATCCAGACCAAGGATTACTTTTTCt                                                                                                                     >  2:3493454/1‑150 (MQ=255)
                               aaGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATGTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAATACCACCGCCCCGGGTGTGATGATCCAGCCCAAGGGGTGCTTTTGCTgg                                                                                                                   >  2:2820299/1‑149 (MQ=255)
                                aGTACGGCTTCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTAATTTTTCTGTa                                                                                                                  >  1:1394964/1‑150 (MQ=255)
                                aGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTa                                                                                                                  >  1:5239317/1‑150 (MQ=255)
                                aGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTACACCAGGAACCACACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGATTACTTTTTCTGTa                                                                                                                  >  2:2409086/1‑150 (MQ=255)
                                       cTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGACCCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAAcaca                                                                                                           >  2:4511803/1‑150 (MQ=255)
                                           gTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGaaa                                                                                                       >  2:2724532/1‑150 (MQ=255)
                                            tCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGaaaa                                                                                                      <  1:1112261/150‑1 (MQ=255)
                                            tCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGaaaa                                                                                                      >  2:3154328/1‑150 (MQ=255)
                                             ccTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAAt                                                                                                     >  1:342451/1‑150 (MQ=255)
                                              cTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATc                                                                                                    <  1:1869513/150‑1 (MQ=255)
                                              cTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATc                                                                                                    <  1:2838209/150‑1 (MQ=255)
                                               tCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCa                                                                                                   >  2:1283199/1‑150 (MQ=255)
                                                cATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACAAGGAACCAGACAATATTAGGAAAAACCACCGCCCCAGTTTTTATTATCCAGACCAATGTTTACCTTTTCTGTACAACACAGAAAATCAc                                                                                                  >  1:1577653/1‑150 (MQ=255)
                                                 aTAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCAcc                                                                                                 >  1:2003994/1‑150 (MQ=255)
                                                    aTCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTg                                                                                              <  1:3654254/150‑1 (MQ=255)
                                                     tCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGt                                                                                             >  2:5089321/1‑150 (MQ=255)
                                                       aaCACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTc                                                                                           <  2:1022331/150‑1 (MQ=255)
                                                        acacCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTca                                                                                          <  2:1957041/150‑1 (MQ=255)
                                                         cacCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTcac                                                                                         >  1:4226769/1‑150 (MQ=255)
                                                          acCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTcaca                                                                                        <  1:1727705/150‑1 (MQ=255)
                                                            cATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAgg                                                                                      >  2:4715241/1‑150 (MQ=255)
                                                             aTCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGc                                                                                     >  1:4628564/1‑150 (MQ=255)
                                                             aTCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTCTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGc                                                                                     >  2:3253279/1‑150 (MQ=255)
                                                               cAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCg                                                                                   <  2:1843568/150‑1 (MQ=255)
                                                                aGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCgg                                                                                  >  2:2635195/1‑150 (MQ=255)
                                                                    aTTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACaa                                                                              >  1:2211715/1‑150 (MQ=255)
                                                                         ccACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATTTTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACAcc                                                                         <  1:1239831/150‑1 (MQ=255)
                                                                         ccACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACAcc                                                                         <  1:3076482/150‑1 (MQ=255)
                                                                            caACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCa                                                                      >  2:754219/1‑150 (MQ=255)
                                                                             aaCGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCaa                                                                     >  1:2936944/1‑150 (MQ=255)
                                                                               cGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAAc                                                                   <  1:3817927/150‑1 (MQ=255)
                                                                                 cATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGt                                                                 >  2:4999897/1‑150 (MQ=255)
                                                                                     aCGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGAcc                                                             <  2:1658755/150‑1 (MQ=255)
                                                                                     aCGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGAcc                                                             <  1:1440681/150‑1 (MQ=255)
                                                                                     aCGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGAcc                                                             <  2:2303317/150‑1 (MQ=255)
                                                                                     aCGATGAATTGTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGAcc                                                             <  1:3928577/150‑1 (MQ=255)
                                                                                       gATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGc                                                           >  2:3044623/1‑150 (MQ=255)
                                                                                       gATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGc                                                           >  1:656251/1‑150 (MQ=255)
                                                                                        aTGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCt                                                          >  1:2809461/1‑150 (MQ=255)
                                                                                          gAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGt                                                        <  2:4371595/150‑1 (MQ=255)
                                                                                          gAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGt                                                        >  2:3624412/1‑150 (MQ=255)
                                                                                           aaTTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGtt                                                       >  2:2429031/1‑150 (MQ=255)
                                                                                            aTTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTa                                                      >  2:1681146/1‑150 (MQ=255)
                                                                                             ttttGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTAt                                                     <  2:5309185/150‑1 (MQ=255)
                                                                                                tGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCg                                                  <  1:569767/150‑1 (MQ=255)
                                                                                                  agaATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGAt                                                >  1:261080/1‑150 (MQ=255)
                                                                                                  agaATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGAt                                                >  1:4961010/1‑150 (MQ=255)
                                                                                                     aTTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATacg                                             >  2:3753862/1‑150 (MQ=255)
                                                                                                          aCCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGAcgcg                                        >  2:2016106/1‑150 (MQ=255)
                                                                                                            cAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGtt                                      >  2:5155686/1‑150 (MQ=255)
                                                                                                            cAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGtt                                      <  1:1615937/150‑1 (MQ=255)
                                                                                                              ggAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGc                                    <  2:5265971/150‑1 (MQ=255)
                                                                                                                   cAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCAc                               <  2:2211715/150‑1 (MQ=255)
                                                                                                                    aGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACt                              >  2:3892861/1‑150 (MQ=255)
                                                                                                                       cAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTc                           <  2:880573/150‑1 (MQ=255)
                                                                                                                        aaTATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTcc                          >  2:4937119/1‑150 (MQ=255)
                                                                                                                        aaTATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTcc                          <  1:4192376/150‑1 (MQ=255)
                                                                                                                        aaTATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTcc                          >  2:938834/1‑150 (MQ=255)
                                                                                                                             ttGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCt                     <  1:4709019/150‑1 (MQ=255)
                                                                                                                               ggAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCttt                   <  1:1173275/150‑1 (MQ=255)
                                                                                                                                gAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTg                  <  1:4372896/150‑1 (MQ=255)
                                                                                                                                gAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTg                  <  1:2773022/150‑1 (MQ=255)
                                                                                                                                 aaaaaCCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCTCCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGa                 <  2:4793191/150‑1 (MQ=255)
                                                                                                                                 aaaaaCCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCCCTTTCCAGTCTTTGa                 >  2:2786184/1‑150 (MQ=255)
                                                                                                                                 aaaaaCCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGa                 <  1:3327019/150‑1 (MQ=255)
                                                                                                                                 aaaaaCCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGa                 <  1:84881/150‑1 (MQ=255)
                                                                                                                                   aaaCCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACt               >  1:2894616/1‑150 (MQ=255)
                                                                                                                                   aaaCCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGAGACGACGCGTTGCCCCACTTTCCAGTCTTTGACt               >  2:3018395/1‑150 (MQ=255)
                                                                                                                                    aaCCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACtt              >  2:1163320/1‑150 (MQ=255)
                                                                                                                                     accaccGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACttt             <  2:1474253/150‑1 (MQ=255)
                                                                                                                                      ccaccGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACtttt            >  1:4141785/1‑150 (MQ=255)
                                                                                                                                             cccAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCaa     >  2:4336823/1‑150 (MQ=255)
                                                                                                                                             cccAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTATGACTTTTTCACCaa     >  2:4044816/1‑150 (MQ=255)
                                                                                                                                              ccAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAAc    >  2:405360/1‑150 (MQ=255)
                                                                                                                                              ccAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAAc    >  1:684932/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCT  >  CP009273/1855091‑1855384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCTGCGC  >  CP009273/1855091‑1855388
                                                                                                                                                    |                                                                                                                                                     
CGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTT                                                                                                                                                      <  SRR19973062.1125652/150‑1 (MQ=60)
CGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTAT                                                                                                                                                      >  SRR19973062.4891322/1‑150 (MQ=60)
 GATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTT                                                                                                                                                     >  SRR19973062.863491/1‑150 (MQ=60)
   TGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCACCACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTAT                                                                                                                                                   <  SRR19973062.1055775/150‑1 (MQ=60)
   TGGATTTGTAGGAATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCAGGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTAT                                                                                                                                                   <  SRR19973062.5016483/150‑1 (MQ=60)
      ACTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTAT                                                                                                                                                <  SRR19973062.5119970/150‑1 (MQ=60)
       TTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATC                                                                                                                                               <  SRR19973062.4006326/150‑1 (MQ=60)
       TTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGAAGATGAATTTTGAGAATTTCACCAGTAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATC                                                                                                                                               <  SRR19973062.3498228/150‑1 (MQ=60)
         TGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTTAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCA                                                                                                                                             >  SRR19973062.2397252/1‑150 (MQ=60)
         TGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCA                                                                                                                                             <  SRR19973062.2242549/150‑1 (MQ=60)
         TGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCA                                                                                                                                             >  SRR19973062.2315353/1‑150 (MQ=60)
          GTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAG                                                                                                                                            <  SRR19973062.2930510/150‑1 (MQ=60)
          GTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAG                                                                                                                                            <  SRR19973062.84867/150‑1 (MQ=60)
           TAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGA                                                                                                                                           <  SRR19973062.584138/150‑1 (MQ=60)
           TAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGA                                                                                                                                           <  SRR19973062.2572254/150‑1 (MQ=60)
           TAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGA                                                                                                                                           <  SRR19973062.2572333/150‑1 (MQ=60)
           TAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGA                                                                                                                                           <  SRR19973062.137556/150‑1 (MQ=60)
             GGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACC                                                                                                                                         >  SRR19973062.1328032/1‑150 (MQ=60)
               CATGACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACACTATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAA                                                                                                                                       >  SRR19973062.4818067/1‑150 (MQ=60)
                ATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCATGAACCAGACAATATTTGGAAAAACCACAGCCCCAGGTTTTATTATCCAGACCAAG                                                                                                                                      >  SRR19973062.2359671/1‑150 (MQ=60)
                ATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAG                                                                                                                                      >  SRR19973062.5195318/1‑150 (MQ=60)
                ATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAG                                                                                                                                      >  SRR19973062.4351487/1‑150 (MQ=60)
                ATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAG                                                                                                                                      >  SRR19973062.4351078/1‑150 (MQ=60)
                 ttaattttatgttaaagatctgctgataacacataataaaaacaatcagggttttaccacaaagcatgtcgattaattttgactttttaaccagtaacatGACAATATTTGGAAAAACCACCTCCCCAGGTTTTATTATCCAGACCAAGG                                                                                                                                     <  SRR19973062.1384668/50‑1 (MQ=60)
                 TTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGG                                                                                                                                     <  SRR19973062.2301918/150‑1 (MQ=60)
                   ACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTT                                                                                                                                   >  SRR19973062.3299771/1‑150 (MQ=60)
                    CAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGTATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTT                                                                                                                                  <  SRR19973062.1621177/150‑1 (MQ=60)
                    CAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTT                                                                                                                                  <  SRR19973062.756488/150‑1 (MQ=60)
                    CAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTT                                                                                                                                  >  SRR19973062.1373801/1‑150 (MQ=60)
                      GATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTAC                                                                                                                                <  SRR19973062.3096142/150‑1 (MQ=60)
                       ATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACT                                                                                                                               <  SRR19973062.4912937/150‑1 (MQ=60)
                       ATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACT                                                                                                                               >  SRR19973062.667363/1‑150 (MQ=60)
                       ATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACT                                                                                                                               >  SRR19973062.4896486/1‑150 (MQ=60)
                       ATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACT                                                                                                                               <  SRR19973062.5147699/150‑1 (MQ=60)
                       ATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACT                                                                                                                               <  SRR19973062.5147762/150‑1 (MQ=60)
                          GGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTT                                                                                                                            <  SRR19973062.2622999/150‑1 (MQ=60)
                            GTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTC                                                                                                                          <  SRR19973062.4303377/150‑1 (MQ=60)
                             TCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGTTTTACTTTTTCT                                                                                                                         >  SRR19973062.4045728/1‑150 (MQ=60)
                               AAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGT                                                                                                                       >  SRR19973062.3739460/1‑150 (MQ=60)
                                AGTACGGCTTCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTAATTTTTCTGTA                                                                                                                      >  SRR19973062.1401582/1‑150 (MQ=60)
                                AGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTA                                                                                                                      >  SRR19973062.5266918/1‑150 (MQ=60)
                                AGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTA                                                                                                                      <  SRR19973062.2992721/150‑1 (MQ=60)
                                AGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTA                                                                                                                      >  SRR19973062.3975707/1‑150 (MQ=60)
                                    CGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAAC                                                                                                                  <  SRR19973062.1447226/150‑1 (MQ=60)
                                            TCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAA                                                                                                          <  SRR19973062.1117483/150‑1 (MQ=60)
                                            TCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAA                                                                                                          <  SRR19973062.3399691/150‑1 (MQ=60)
                                             CCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAAT                                                                                                         >  SRR19973062.1419200/1‑150 (MQ=60)
                                             CCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAAT                                                                                                         >  SRR19973062.344028/1‑150 (MQ=60)
                                              CTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATC                                                                                                        >  SRR19973062.4727051/1‑150 (MQ=60)
                                              CTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATC                                                                                                        >  SRR19973062.3411391/1‑150 (MQ=60)
                                              CTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATC                                                                                                        <  SRR19973062.1878939/150‑1 (MQ=60)
                                              CTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATC                                                                                                        <  SRR19973062.2853221/150‑1 (MQ=60)
                                               TCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCA                                                                                                       >  SRR19973062.2806399/1‑150 (MQ=60)
                                                CATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACAAGGAACCAGACAATATTAGGAAAAACCACCGCCCCAGTTTTTATTATCCAGACCAATGTTTACCTTTTCTGTACAACACAGAAAATCAC                                                                                                      >  SRR19973062.1585461/1‑150 (MQ=60)
                                                 ATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACC                                                                                                     >  SRR19973062.2014295/1‑150 (MQ=60)
                                                 ATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGATTTATTATCCAGACCAAGGATTACTTTTTCTGTACAACAAAGAAAATCACC                                                                                                     >  SRR19973062.2825400/1‑150 (MQ=60)
                                                 ATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAAATTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGAACAAGGTTTACTTTTTCTGTACAACACAGAAAATCACC                                                                                                     >  SRR19973062.855676/1‑150 (MQ=60)
                                                   AATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTT                                                                                                   <  SRR19973062.2290618/150‑1 (MQ=60)
                                                   AATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTT                                                                                                   >  SRR19973062.4993500/1‑150 (MQ=60)
                                                   AATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTT                                                                                                   <  SRR19973062.1859503/150‑1 (MQ=60)
                                                    ATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTG                                                                                                  <  SRR19973062.3672983/150‑1 (MQ=60)
                                                     TCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGT                                                                                                 <  SRR19973062.2855995/150‑1 (MQ=60)
                                                      CAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTT                                                                                                <  SRR19973062.570378/150‑1 (MQ=60)
                                                       AACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAGGATTTTCTTTTTCTGTACAACACAGAAAATCACCTTGTTC                                                                                               <  SRR19973062.4182702/150‑1 (MQ=60)
                                                       AACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTC                                                                                               >  SRR19973062.5041543/1‑150 (MQ=60)
                                                         CACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCAC                                                                                             >  SRR19973062.4248673/1‑150 (MQ=60)
                                                         CACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCAC                                                                                             <  SRR19973062.368976/150‑1 (MQ=60)
                                                          ACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACA                                                                                            <  SRR19973062.1736124/150‑1 (MQ=60)
                                                          ACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTGGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACA                                                                                            <  SRR19973062.1736336/150‑1 (MQ=60)
                                                           CCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAG                                                                                           <  SRR19973062.476628/150‑1 (MQ=60)
                                                            CATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGG                                                                                          >  SRR19973062.4848614/1‑150 (MQ=60)
                                                             ATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGC                                                                                         >  SRR19973062.4652787/1‑150 (MQ=60)
                                                               CAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCG                                                                                       <  SRR19973062.4394726/150‑1 (MQ=60)
                                                                    ATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAA                                                                                  >  SRR19973062.2222797/1‑150 (MQ=60)
                                                                        CCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACAC                                                                              >  SRR19973062.3968792/1‑150 (MQ=60)
                                                                         CCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATTTTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACC                                                                             <  SRR19973062.1245577/150‑1 (MQ=60)
                                                                         CCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATTTTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACC                                                                             <  SRR19973062.1245620/150‑1 (MQ=60)
                                                                         CCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATTTTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACC                                                                             <  SRR19973062.1245646/150‑1 (MQ=60)
                                                                         CCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACC                                                                             <  SRR19973062.3858500/150‑1 (MQ=60)
                                                                         CCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACC                                                                             <  SRR19973062.3092403/150‑1 (MQ=60)
                                                                          CACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCG                                                                            >  SRR19973062.4266766/1‑150 (MQ=60)
                                                                             AACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAA                                                                         >  SRR19973062.2952400/1‑150 (MQ=60)
                                                                               CGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAAC                                                                       <  SRR19973062.3837453/150‑1 (MQ=60)
                                                                                     ACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACC                                                                 <  SRR19973062.3836702/150‑1 (MQ=60)
                                                                                     ACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACC                                                                 <  SRR19973062.1447708/150‑1 (MQ=60)
                                                                                     ACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACC                                                                 <  SRR19973062.1447676/150‑1 (MQ=60)
                                                                                     ACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACC                                                                 >  SRR19973062.2807829/1‑150 (MQ=60)
                                                                                     ACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACC                                                                 <  SRR19973062.1447675/150‑1 (MQ=60)
                                                                                     ACGATGAATTGTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACC                                                                 <  SRR19973062.3948550/150‑1 (MQ=60)
                                                                                      CGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCG                                                                >  SRR19973062.3077771/1‑150 (MQ=60)
                                                                                       GATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGC                                                               >  SRR19973062.3327757/1‑150 (MQ=60)
                                                                                       GATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGC                                                               >  SRR19973062.3327740/1‑150 (MQ=60)
                                                                                       GATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGC                                                               >  SRR19973062.659313/1‑150 (MQ=60)
                                                                                        ATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCT                                                              <  SRR19973062.2147535/150‑1 (MQ=60)
                                                                                        ATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCT                                                              >  SRR19973062.2824324/1‑150 (MQ=60)
                                                                                          GAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGT                                                            >  SRR19973062.4644296/1‑150 (MQ=60)
                                                                                           AATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTT                                                           <  SRR19973062.4166697/150‑1 (MQ=60)
                                                                                           AATTTTGAGAATTTCACCAGGAACCAGACAATATATGGAAAAACCACCGCACCAGGTATTATTATCCAGACAAAGGTTTACTTTTTCTGTAAAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCACACGTGACCGCTGTT                                                           >  SRR19973062.2052185/1‑150 (MQ=60)
                                                                                            ATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTA                                                          >  SRR19973062.1161679/1‑150 (MQ=60)
                                                                                                TGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCG                                                      <  SRR19973062.572441/150‑1 (MQ=60)
                                                                                                TGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCG                                                      <  SRR19973062.572403/150‑1 (MQ=60)
                                                                                                TGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCG                                                      >  SRR19973062.2607524/1‑150 (MQ=60)
                                                                                                  AGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGAT                                                    >  SRR19973062.262389/1‑150 (MQ=60)
                                                                                                  AGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGAT                                                    >  SRR19973062.4987097/1‑150 (MQ=60)
                                                                                                    AATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACGACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATAC                                                  >  SRR19973062.2665340/1‑150 (MQ=60)
                                                                                                     ATTACACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACG                                                 >  SRR19973062.1076296/1‑150 (MQ=60)
                                                                                                           CCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGT                                           >  SRR19973062.4232593/1‑150 (MQ=60)
                                                                                                           CCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGT                                           <  SRR19973062.4122845/150‑1 (MQ=60)
                                                                                                            CAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTT                                          <  SRR19973062.1623935/150‑1 (MQ=60)
                                                                                                             AGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTG                                         >  SRR19973062.594349/1‑150 (MQ=60)
                                                                                                                AACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGATAACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCC                                      >  SRR19973062.3855012/1‑150 (MQ=60)
                                                                                                                AACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCC                                      >  SRR19973062.4395512/1‑150 (MQ=60)
                                                                                                                   CAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCAC                                   >  SRR19973062.3525877/1‑150 (MQ=60)
                                                                                                                    AGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACT                                  <  SRR19973062.2658418/150‑1 (MQ=60)
                                                                                                                      ACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTT                                >  SRR19973062.1681630/1‑150 (MQ=60)
                                                                                                                       CAATATTTGGAAAAACCACCGCCCCAGGTATTAATATCCAGACCAAGGTTAACTTATTCTGTACAAAACAGAAAATCACCTTGTTCACAGGCCTGACAAACACCGCAAAAGTGACCGCTGTTATCCGATACGAAGCGTTGacacacattc                               >  SRR19973062.499660/1‑140 (MQ=60)
                                                                                                                        AATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCC                              <  SRR19973062.4214121/150‑1 (MQ=60)
                                                                                                                          TATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAG                            >  SRR19973062.4662729/1‑150 (MQ=60)
                                                                                                                             TTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCT                         <  SRR19973062.4733605/150‑1 (MQ=60)
                                                                                                                              TGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGAGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTGCCAGTCTT                        >  SRR19973062.2916911/1‑150 (MQ=60)
                                                                                                                               GGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTT                       <  SRR19973062.1178670/150‑1 (MQ=60)
                                                                                                                               GGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTT                       <  SRR19973062.1178767/150‑1 (MQ=60)
                                                                                                                               GGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTT                       <  SRR19973062.1384546/150‑1 (MQ=60)
                                                                                                                               GGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTT                       >  SRR19973062.2889726/1‑150 (MQ=60)
                                                                                                                                GAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTG                      <  SRR19973062.4395331/150‑1 (MQ=60)
                                                                                                                                GAAAAACCACCGACCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTG                      <  SRR19973062.2787679/150‑1 (MQ=60)
                                                                                                                                 AAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGA                     <  SRR19973062.3344199/150‑1 (MQ=60)
                                                                                                                                 AAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGA                     >  SRR19973062.4870583/1‑150 (MQ=60)
                                                                                                                                 AAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGA                     <  SRR19973062.85450/150‑1 (MQ=60)
                                                                                                                                  AAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGAC                    >  SRR19973062.1207940/1‑150 (MQ=60)
                                                                                                                                   AAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACT                   >  SRR19973062.2909889/1‑150 (MQ=60)
                                                                                                                                      CCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTT                >  SRR19973062.4163254/1‑150 (MQ=60)
                                                                                                                                       CACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTT               >  SRR19973062.5208144/1‑150 (MQ=60)
                                                                                                                                       CACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTT               <  SRR19973062.1902096/150‑1 (MQ=60)
                                                                                                                                       CACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTT               <  SRR19973062.1989655/150‑1 (MQ=60)
                                                                                                                                           GCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCACACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACC           >  SRR19973062.1380748/1‑150 (MQ=60)
                                                                                                                                              CCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAAC        >  SRR19973062.5239460/1‑150 (MQ=60)
                                                                                                                                              CCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAAC        >  SRR19973062.1587066/1‑150 (MQ=60)
                                                                                                                                              CCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAAC        >  SRR19973062.688104/1‑150 (MQ=60)
                                                                                                                                                AGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCT      >  SRR19973062.1224243/1‑150 (MQ=60)
                                                                                                                                                   TTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCTGCG   <  SRR19973062.4378565/150‑1 (MQ=60)
                                                                                                                                                   TTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCTGCG   >  SRR19973062.4619087/1‑150 (MQ=60)
                                                                                                                                                    TTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCTGCGC  >  SRR19973062.1191351/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCTGCGC  >  CP009273/1855091‑1855388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑