Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,858,693 T→A 19.9% L196Q (CTG→CAG)  yeaD → D‑hexose‑6‑phosphate epimerase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,858,6930TA19.9% 677.4 / 160.3 297L196Q (CTG→CAG) yeaDD‑hexose‑6‑phosphate epimerase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (149/88);  new base A (27/32);  total (176/121)
Fisher's exact test for biased strand distribution p-value = 1.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

Only 100 of 302 total aligned reads displayed.

TTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGA  >  CP009273/1858544‑1858841
                                                                                                                                                     |                                                                                                                                                    
ttGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACt                                                                                                                                                      <  1:1061813/150‑1 (MQ=255)
    gAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGAcc                                                                                                                                                  >  1:1321312/1‑150 (MQ=255)
    gAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGAcc                                                                                                                                                  >  2:1218636/1‑150 (MQ=255)
     aaaTCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCg                                                                                                                                                 >  1:4867263/1‑150 (MQ=255)
      aaTCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGa                                                                                                                                                >  2:4656790/1‑150 (MQ=255)
      aaTCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGa                                                                                                                                                >  2:2623763/1‑150 (MQ=255)
       aTCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGAt                                                                                                                                               >  1:3322424/1‑150 (MQ=255)
         cGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATgg                                                                                                                                             <  2:5261862/150‑1 (MQ=255)
          gATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGt                                                                                                                                            <  2:5295913/150‑1 (MQ=255)
          gATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGt                                                                                                                                            <  2:4330190/150‑1 (MQ=255)
          gATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGt                                                                                                                                            >  2:1991785/1‑150 (MQ=255)
           aTCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTa                                                                                                                                           >  2:3344203/1‑150 (MQ=255)
           aTCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTa                                                                                                                                           >  2:1667637/1‑150 (MQ=255)
             cTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACAGACCGATGGTAtt                                                                                                                                         <  1:1119455/150‑1 (MQ=255)
                 aaTCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACAGACCGATGGTATTCAGa                                                                                                                                     >  2:2052130/1‑150 (MQ=255)
                    cacaTGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCt                                                                                                                                  >  1:3009977/1‑150 (MQ=255)
                      caTGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTc                                                                                                                                >  1:1605606/1‑150 (MQ=255)
                             aaTTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGAcc                                                                                                                         >  1:2917575/1‑150 (MQ=255)
                              aTTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACAGACCGATGGTATTCAGACCTTCCCTGACCg                                                                                                                        >  2:559615/1‑150 (MQ=255)
                                 tGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTAc                                                                                                                     <  1:3614795/150‑1 (MQ=255)
                                 tGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTAc                                                                                                                     >  2:488427/1‑150 (MQ=255)
                                 tGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTAc                                                                                                                     <  1:3000485/150‑1 (MQ=255)
                                 tGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACAGACCGATGGTATTCAGACCTTCCCTGACCGTAc                                                                                                                     >  2:5024041/1‑150 (MQ=255)
                                  gAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTAcc                                                                                                                    <  1:4948791/150‑1 (MQ=255)
                                  gAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTAcc                                                                                                                    <  2:1864413/150‑1 (MQ=255)
                                  gAAAACACCTCTGCCCTGCATACCTACTTTAAAGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTACATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTAcc                                                                                                                    <  2:3779279/150‑1 (MQ=255)
                                      ccaccTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATc                                                                                                                <  1:3088066/150‑1 (MQ=255)
                                             tGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTa                                                                                                         >  2:2261829/1‑150 (MQ=255)
                                              gCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTAt                                                                                                        <  1:282350/150‑1 (MQ=255)
                                                 cTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTg                                                                                                     <  2:1935907/150‑1 (MQ=255)
                                                    cATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAAt                                                                                                  <  1:4612313/150‑1 (MQ=255)
                                                     aTACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATc                                                                                                 >  2:3306394/1‑150 (MQ=255)
                                                       aCCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTTAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACAGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCca                                                                                               >  1:2368879/1‑150 (MQ=255)
                                                       aCCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCca                                                                                               <  2:4136000/150‑1 (MQ=255)
                                                         cTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCcaca                                                                                             >  1:5270707/1‑150 (MQ=255)
                                                          tACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACaa                                                                                            >  2:1799947/1‑150 (MQ=255)
                                                            cTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGa                                                                                          >  2:4304285/1‑150 (MQ=255)
                                                               tAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTg                                                                                       >  1:267057/1‑150 (MQ=255)
                                                                  cGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAg                                                                                    >  2:4782869/1‑150 (MQ=255)
                                                                    tGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCg                                                                                  >  1:253254/1‑150 (MQ=255)
                                                                     gggTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGt                                                                                 >  1:3220601/1‑150 (MQ=255)
                                                                        tGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGAt                                                                              >  1:4242629/1‑150 (MQ=255)
                                                                        tGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACAGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGAt                                                                              <  1:3337737/150‑1 (MQ=255)
                                                                          atatCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTa                                                                            >  2:1432882/1‑150 (MQ=255)
                                                                              cGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAatga                                                                        <  2:1024655/150‑1 (MQ=255)
                                                                               gCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTActgat                                                                       <  1:528472/150‑1 (MQ=255)
                                                                                cTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAatgatg                                                                      >  2:2360622/1‑150 (MQ=255)
                                                                                 tAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAAtgatga                                                                     >  2:3566852/1‑150 (MQ=255)
                                                                                  aaGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGaa                                                                    >  1:962419/1‑150 (MQ=255)
                                                                                           gTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAAt                                                           <  2:4959392/150‑1 (MQ=255)
                                                                                           gTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACAGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAAt                                                           >  2:3765879/1‑150 (MQ=255)
                                                                                           gTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACAGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAAt                                                           <  1:1220651/150‑1 (MQ=255)
                                                                                            tCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATc                                                          >  2:347860/1‑150 (MQ=255)
                                                                                                tgggctgggcGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGtat                                                      >  1:5169301/1‑150 (MQ=255)
                                                                                                  ggctgggcGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACAGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGtatta                                                    <  2:5218945/150‑1 (MQ=255)
                                                                                                    ctgggcGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACAGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATc                                                  <  1:2052130/150‑1 (MQ=255)
                                                                                                     tgggcGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCg                                                 >  2:2795315/1‑150 (MQ=255)
                                                                                                      gggcGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGc                                                >  1:2134541/1‑150 (MQ=255)
                                                                                                         cGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGt                                             >  2:3118290/1‑150 (MQ=255)
                                                                                                           aTCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACAGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAg                                           <  2:5029336/150‑1 (MQ=255)
                                                                                                              gCTTCATTGATAAAGTGAATGACGCGAAAGAAAATTTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGcc                                        >  2:626728/1‑150 (MQ=255)
                                                                                                              gCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGcc                                        <  1:913080/150‑1 (MQ=255)
                                                                                                              gCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGcc                                        >  2:3144978/1‑150 (MQ=255)
                                                                                                               cTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACAGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGcca                                       >  1:1429249/1‑150 (MQ=255)
                                                                                                                ttCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGccac                                      >  1:3982332/1‑150 (MQ=255)
                                                                                                                 tCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGccacc                                     >  2:1605596/1‑150 (MQ=255)
                                                                                                                  cATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGccacca                                    >  1:4214648/1‑150 (MQ=255)
                                                                                                                   aTTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAg                                   >  2:3104738/1‑150 (MQ=255)
                                                                                                                     tGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACGGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGca                                 >  1:4342777/1‑150 (MQ=255)
                                                                                                                       aTAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGcatc                               <  2:4892452/150‑1 (MQ=255)
                                                                                                                         aaaGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGcatcat                             >  2:2121014/1‑150 (MQ=255)
                                                                                                                         aaaGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGcatcat                             <  2:4700602/150‑1 (MQ=255)
                                                                                                                         aaaGTGAATGACGCGAAAGAAAATGTACAGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGcatcat                             <  1:4983734/150‑1 (MQ=255)
                                                                                                                          aaGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCatcatc                            <  2:4223157/150‑1 (MQ=255)
                                                                                                                           aGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCt                           <  1:3127578/150‑1 (MQ=255)
                                                                                                                           aGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCt                           <  1:4551509/150‑1 (MQ=255)
                                                                                                                           aGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCt                           >  2:4388022/1‑150 (MQ=255)
                                                                                                                            gTGAATGACGCGAAAGAAAATGTACAGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTg                          >  2:2941253/1‑150 (MQ=255)
                                                                                                                               aaTGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCGTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAAc                       <  1:568525/150‑1 (MQ=255)
                                                                                                                               aaTGACGCGAAAGAAAATGTACAGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAAc                       >  2:835846/1‑150 (MQ=255)
                                                                                                                                  gACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGtt                    >  2:91540/1‑150 (MQ=255)
                                                                                                                                  gACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGtt                    <  2:3742497/150‑1 (MQ=255)
                                                                                                                                  gACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCAACTGAACGtt                    <  1:1957527/150‑1 (MQ=255)
                                                                                                                                   aCGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTg                   <  1:1844552/150‑1 (MQ=255)
                                                                                                                                     gcgAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTc                 <  2:191225/150‑1 (MQ=255)
                                                                                                                                       gaaagaaaATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCgg               >  2:4571329/1‑150 (MQ=255)
                                                                                                                                       gaaagaaaATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCgg               >  1:1251445/1‑150 (MQ=255)
                                                                                                                                       gaaagaaaATGTACAGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCgg               >  1:822918/1‑150 (MQ=255)
                                                                                                                                        aaagaaaATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGc              >  2:3927622/1‑150 (MQ=255)
                                                                                                                                          agaaaATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTg            >  2:4030147/1‑150 (MQ=255)
                                                                                                                                          agaaaATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTg            >  2:3846138/1‑150 (MQ=255)
                                                                                                                                          agaaaATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTg            >  1:4059213/1‑150 (MQ=255)
                                                                                                                                          agaaaATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTg            >  1:4430388/1‑150 (MQ=255)
                                                                                                                                          agaaaATGTACAGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTg            <  1:5024275/150‑1 (MQ=255)
                                                                                                                                            aaaaTGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGa          >  1:3542317/1‑150 (MQ=255)
                                                                                                                                            aaaaTGTACTGACCGATGGTATTCAGACCTTACCACACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATAAAAGAAGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGa          >  1:1861015/1‑150 (MQ=255)
                                                                                                                                               aTGTACAGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAAcc       <  2:4622817/150‑1 (MQ=255)
                                                                                                                                                 gTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGACCCCg     >  2:175287/1‑150 (MQ=255)
                                                                                                                                                 gTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCg     >  1:2232246/1‑150 (MQ=255)
                                                                                                                                                    cTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGa  >  2:247161/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATCCACAAGATTGCAGCGTGATTAATGATGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGA  >  CP009273/1858544‑1858841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A