Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,859,132 A→C 100% A254A (GCT→GCG)  yeaE ← aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,859,1320AC99.2% 952.0 / ‑4.0 239A254A (GCT→GCG) yeaEaldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (118/119);  total (119/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 245 total aligned reads displayed.

ACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAG  >  CP009273/1858985‑1859278
                                                                                                                                                   |                                                                                                                                                  
aCGTTAATTTACTTTAATGTTGTGTGCCGGGTGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGc                                                                                                                                                  <  1:1254869/150‑1 (MQ=255)
 cGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGcc                                                                                                                                                 >  2:3315053/1‑150 (MQ=255)
  gctactttACGTGAATGTTGTGTGCCGGGTGCAACGCAGCCGGCACACAACATCACACCAGATCCCGCGCAGTATTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATACCGCTGAAGAAAGTTCGACCTCACGCAccgccg                                                                                                                                                <  2:3982332/145‑1 (MQ=255)
  gTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACAAAACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATTCCTTATCCAGCATAGCTAATTCCGCTGAATAAAGTTAGACCTCAAGCAccgccg                                                                                                                                                >  1:2202450/1‑150 (MQ=255)
      aTTTCCGTTAATGTGGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCAtt                                                                                                                                            <  1:4897553/150‑1 (MQ=255)
       tttATGTTATTTTTGTGTGCCGGGTGCATTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCAttt                                                                                                                                           <  1:2798626/150‑1 (MQ=255)
           cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTttgtt                                                                                                                                       <  2:1661352/150‑1 (MQ=255)
            gTTAATGTTGTGTGCCGGGTGCAATGCAACCGGCACACAACAACACACCAAATACAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTttgttg                                                                                                                                      >  2:5164854/1‑150 (MQ=255)
             ttAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTgg                                                                                                                                     <  2:2089846/150‑1 (MQ=255)
                  gttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATg                                                                                                                                >  1:3987998/1‑150 (MQ=255)
                  gttgtGTGCCGGGTGCAATGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATg                                                                                                                                <  1:3724912/150‑1 (MQ=255)
                   ttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATgg                                                                                                                               <  2:5053866/150‑1 (MQ=255)
                    tgtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATggg                                                                                                                              <  2:408497/150‑1 (MQ=255)
                     gtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGc                                                                                                                             >  2:3957547/1‑150 (MQ=255)
                       gtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCaa                                                                                                                           >  1:1543559/1‑150 (MQ=255)
                        ggCCGGGTGCACGGCAGCCGCCCCACACCATCCCACCCTATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCCAGCACCGCCGCATTTTGTTGGACATGGGCTAt                                                                                                                          <  1:4320511/149‑1 (MQ=255)
                             tgTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTgg                                                                                                                     >  2:1282644/2‑150 (MQ=255)
                             ggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTgg                                                                                                                     >  2:2337356/1‑150 (MQ=255)
                              gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGc                                                                                                                    >  1:2475484/1‑150 (MQ=255)
                              gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGc                                                                                                                    >  1:4675099/1‑150 (MQ=255)
                                  aaTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCt                                                                                                                >  1:4360815/1‑150 (MQ=255)
                                       aTCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTgg                                                                                                           <  2:5236533/150‑1 (MQ=255)
                                           ggCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATc                                                                                                       >  2:4420647/1‑150 (MQ=255)
                                             cacacaCCATCACCCCCTAGCCAGCGCAGTGTTTCCTTTTGGTGCCGGATATGCCTTCTCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGc                                                                                                     <  2:2856295/150‑1 (MQ=255)
                                             cacacaACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTGTTGGAATCGc                                                                                                     >  2:4127879/1‑150 (MQ=255)
                                              acacaACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGcc                                                                                                    <  2:1221524/150‑1 (MQ=255)
                                                acaacaTCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCAt                                                                                                  <  2:902129/150‑1 (MQ=255)
                                                         cacCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCtg                                                                                         <  2:4323417/150‑1 (MQ=255)
                                                         cacCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCtg                                                                                         <  1:3205108/150‑1 (MQ=255)
                                                            cATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAATTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCAGCTTTTGGAATAGCCATCACACCCtgatt                                                                                      >  1:230785/1‑149 (MQ=255)
                                                            cATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCtgatg                                                                                      <  1:3268831/150‑1 (MQ=255)
                                                             atatCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCtgatga                                                                                     <  2:4231390/150‑1 (MQ=255)
                                                               atCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAATCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCAGATGACt                                                                                   >  1:3108892/1‑150 (MQ=255)
                                                                  cAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGAt                                                                                <  2:892350/150‑1 (MQ=255)
                                                                  cAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTGGGAATCGCCATCACACCCTGATGACTGAt                                                                                <  2:4621607/150‑1 (MQ=255)
                                                                    gcgcAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCa                                                                              <  2:3136360/150‑1 (MQ=255)
                                                                    gcgcAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCa                                                                              <  1:1984056/150‑1 (MQ=255)
                                                                     cgcAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCAc                                                                             >  2:3202414/1‑150 (MQ=255)
                                                                       cAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCAccc                                                                           <  2:2051518/150‑1 (MQ=255)
                                                                        aGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCa                                                                          <  1:1837641/150‑1 (MQ=255)
                                                                            tttCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGcc                                                                      >  1:1635602/1‑150 (MQ=255)
                                                                             ttCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCca                                                                     <  1:884314/150‑1 (MQ=255)
                                                                                cTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaaca                                                                  >  1:418936/1‑150 (MQ=255)
                                                                                cTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaaca                                                                  >  2:4803127/1‑150 (MQ=255)
                                                                                  tttGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAAt                                                                <  2:785146/150‑1 (MQ=255)
                                                                                    tGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATAc                                                              >  1:5094934/1‑150 (MQ=255)
                                                                                        gCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTtgt                                                          <  1:2122205/150‑1 (MQ=255)
                                                                                        gCCGGATATGCCTTATCCAGCATAGCTAAGTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTtgt                                                          <  1:4371496/150‑1 (MQ=255)
                                                                                         ccGGATATTCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCACGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTtgtg                                                         <  1:1467317/150‑1 (MQ=255)
                                                                                         ccGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTtgtg                                                         >  2:2936153/1‑150 (MQ=255)
                                                                                          cGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGc                                                        >  2:655865/1‑150 (MQ=255)
                                                                                           ggATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGcc                                                       <  2:1454788/150‑1 (MQ=255)
                                                                                            gATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATGCTTGTGCcg                                                      >  1:631368/1‑150 (MQ=255)
                                                                                            gATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCcg                                                      >  1:2624010/1‑150 (MQ=255)
                                                                                              tatGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCcgcg                                                    >  2:4777160/1‑150 (MQ=255)
                                                                                               atGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCgcgc                                                   >  1:3960092/1‑150 (MQ=255)
                                                                                                tGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCt                                                  <  2:2202458/150‑1 (MQ=255)
                                                                                                tGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCt                                                  <  1:3499792/150‑1 (MQ=255)
                                                                                                 gCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTg                                                 >  2:246509/1‑150 (MQ=255)
                                                                                                   cTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCACGCACCGCCGCATTGTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGat                                               <  2:4256460/150‑1 (MQ=255)
                                                                                                     tATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGatat                                             >  2:1934691/1‑150 (MQ=255)
                                                                                                     tATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGatat                                             >  2:1934589/1‑150 (MQ=255)
                                                                                                     tATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGatat                                             >  2:4035557/1‑150 (MQ=255)
                                                                                                     tATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGatat                                             <  2:1577177/150‑1 (MQ=255)
                                                                                                     tATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGatat                                             <  1:3730675/150‑1 (MQ=255)
                                                                                                       tCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGATTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATtg                                           <  1:4213779/150‑1 (MQ=255)
                                                                                                        ccAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATtgt                                          <  1:3024794/150‑1 (MQ=255)
                                                                                                         cAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATtgtg                                         <  2:381965/150‑1 (MQ=255)
                                                                                                           gCATAGCTAATTCCGCAGAAGAAAGTTCCACCTCAAGCCCCGCCGCATTATGTTTGACATGGGCAATCGTGGCCGCTTTTGGAATCGACATCACACCCTGAAGTCTGATCAACCACGCCAACAATACTTGTGCCGCGCTGATATTGTTAg                                       >  2:1142493/1‑150 (MQ=255)
                                                                                                            cATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGc                                      >  1:698915/1‑150 (MQ=255)
                                                                                                             aTAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACATGTGCCGCGCTGATATTGAGAGCa                                     >  2:3529542/1‑150 (MQ=255)
                                                                                                              tAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCAt                                    >  1:570961/1‑150 (MQ=255)
                                                                                                               aGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCAGATGACTGATCACCCACGCCAACAAAACTTGTGCCGCGCTGATATTGTGAGCaag                                   >  1:4980090/1‑148 (MQ=255)
                                                                                                                 cTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCAtttg                                 >  2:4976039/1‑150 (MQ=255)
                                                                                                                 cTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCAtgtg                                 <  1:4762891/150‑1 (MQ=255)
                                                                                                                 cTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCGTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCAtgtg                                 >  2:306178/1‑150 (MQ=255)
                                                                                                                  tAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGc                                >  1:4574652/1‑150 (MQ=255)
                                                                                                                   aaTTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCa                               >  1:294573/1‑150 (MQ=255)
                                                                                                                     ttCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAt                             <  1:2406697/150‑1 (MQ=255)
                                                                                                                      tCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt                            >  1:4456197/1‑150 (MQ=255)
                                                                                                                       ccGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAttt                           <  2:2817089/150‑1 (MQ=255)
                                                                                                                        cGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATAGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTc                          >  2:3657803/1‑150 (MQ=255)
                                                                                                                         gCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCg                         <  1:2072182/150‑1 (MQ=255)
                                                                                                                          cTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGt                        >  2:5189101/1‑150 (MQ=255)
                                                                                                                           tGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGtt                       >  2:4940873/1‑150 (MQ=255)
                                                                                                                            gaagaaAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCACCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTg                      >  2:4549602/1‑150 (MQ=255)
                                                                                                                              agaaAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGAc                    <  1:1733623/150‑1 (MQ=255)
                                                                                                                              agaaAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGAc                    <  1:5205641/150‑1 (MQ=255)
                                                                                                                                 aaGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTAc                 >  1:5160004/1‑150 (MQ=255)
                                                                                                                                  aGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTAcc                <  1:1023598/150‑1 (MQ=255)
                                                                                                                                  aGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTAcc                <  1:3600833/150‑1 (MQ=255)
                                                                                                                                  aGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTAcc                <  2:469481/150‑1 (MQ=255)
                                                                                                                                       gACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGtt           >  2:544739/1‑150 (MQ=255)
                                                                                                                                         ccTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGtttt         <  2:3461818/150‑1 (MQ=255)
                                                                                                                                          cTCAAGCACCGCCGCATTTTGTTGGACATGGGCAAACGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCCACACAACGTGAGCCGCGCTGATATTGTGAGCATGAGCAAATTCGAAGACTACCGCGttatt        >  2:108027/1‑150 (MQ=255)
                                                                                                                                           tCAAGCACCGCCGCATTTTGTTGGACTTGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATTCTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGtttttt       <  2:3267571/150‑1 (MQ=255)
                                                                                                                                             aaGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTaa     >  2:3086868/1‑150 (MQ=255)
                                                                                                                                             aaGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTaa     >  2:3946102/1‑150 (MQ=255)
                                                                                                                                             aaGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTaa     >  2:25673/1‑150 (MQ=255)
                                                                                                                                                caccGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAg  >  2:2691952/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAG  >  CP009273/1858985‑1859278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCC  >  CP009273/1858983‑1859281
                                                                                                                                                     |                                                                                                                                                     
AAACTTTAATTTACTTTATTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACC                                                                                                                                                       <  SRR19973062.2925572/150‑1 (MQ=60)
  ACGTTAATTTACTTTAATGTTGTGTGCCGGGTGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGC                                                                                                                                                     <  SRR19973062.1260663/150‑1 (MQ=60)
    tttaatttttgttttTGTTGTGTGCCGGGTGAATTGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCG                                                                                                                                                   <  SRR19973062.3952083/135‑1 (MQ=60)
    GTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCAAATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGACTTATCCAGCAAAGCTAATTACGCTGAAGAAAGTTAGACCTCAAGAACCGCCG                                                                                                                                                   >  SRR19973062.2213501/1‑150 (MQ=60)
    GTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACAAAACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATTCCTTATCCAGCATAGCTAATTCCGCTGAATAAAGTTAGACCTCAAGCACCGCCG                                                                                                                                                   >  SRR19973062.2213493/1‑150 (MQ=60)
       AATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCCCCCAACATCACACCATATCCCGCGCAGGTTTTGCGTTTGGTGCCGGGTATTCCTTATACAGCCTAGCGAAATCCGCTGAAGAAAGGTCGACCACAAGCACCGCCGCca                                                                                                                                                >  SRR19973062.131034/1‑148 (MQ=60)
        ATTTCCGTTAATGTGGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATT                                                                                                                                               <  SRR19973062.4922747/150‑1 (MQ=60)
         TTTATGTTATTTTTGTGTGCCGGGTGCATTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTT                                                                                                                                              <  SRR19973062.2813430/150‑1 (MQ=60)
            ACGTTAATGTTGTGTGGCGGGTGCAATGCATCCGGCACACAACAACACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCTTATACAGCAAAGCGAATTCCGCTGAAGAAAGTTCGAACTCAAGCACCGCCGCATTTTTT                                                                                                                                           >  SRR19973062.5340314/1‑150 (MQ=60)
              tttattgttgtttgcgggttccaatgaacccgcaaaACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTG                                                                                                                                         <  SRR19973062.4969284/114‑1 (MQ=60)
                    GTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATG                                                                                                                                   >  SRR19973062.4008156/1‑150 (MQ=60)
                    GTTGTGTGCCGGGTGCAATGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATG                                                                                                                                   <  SRR19973062.3744027/150‑1 (MQ=60)
                     TTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGG                                                                                                                                  <  SRR19973062.3998871/150‑1 (MQ=60)
                      TGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGG                                                                                                                                 >  SRR19973062.1569517/1‑150 (MQ=60)
                         GTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAA                                                                                                                              >  SRR19973062.1551162/1‑150 (MQ=60)
                          ggccgggtgcacggcagccgccccacaccatcccacccTATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCCAGCACCGCCGCATTTTGTTGGACATGGGCTAT                                                                                                                             <  SRR19973062.4342782/112‑1 (MQ=60)
                            CCGGGTGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCG                                                                                                                           <  SRR19973062.3204655/150‑1 (MQ=60)
                            CCGGGTCCACTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCG                                                                                                                           <  SRR19973062.513598/150‑1 (MQ=60)
                              GGGTGCAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTG                                                                                                                         <  SRR19973062.171219/150‑1 (MQ=60)
                                GTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGC                                                                                                                       >  SRR19973062.2488745/1‑150 (MQ=60)
                                GTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGC                                                                                                                       >  SRR19973062.4699546/1‑150 (MQ=60)
                                    AATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCT                                                                                                                   >  SRR19973062.282986/1‑150 (MQ=60)
                                    AATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCT                                                                                                                   >  SRR19973062.4383206/1‑150 (MQ=60)
                                    AATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCT                                                                                                                   >  SRR19973062.4074522/1‑150 (MQ=60)
                                    AATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCG                                                                                                                   >  SRR19973062.2374222/1‑150 (MQ=60)
                                    AATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCG                                                                                                                   >  SRR19973062.871918/1‑150 (MQ=60)
                                        aacccgcCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTG                                                                                                               <  SRR19973062.481266/143‑1 (MQ=60)
                                               CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGC                                                                                                        >  SRR19973062.4364901/1‑150 (MQ=60)
                                                ACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCC                                                                                                       >  SRR19973062.306092/1‑150 (MQ=60)
                                                  ACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCCT                                                                                                     >  SRR19973062.1052235/1‑150 (MQ=60)
                                                        TCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACC                                                                                               <  SRR19973062.374559/150‑1 (MQ=60)
                                                           CACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTG                                                                                            <  SRR19973062.3221753/150‑1 (MQ=60)
                                                              CATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAATTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCAGCTTTTGGAATAGCCATCACACCCTGATT                                                                                         >  SRR19973062.231972/1‑150 (MQ=60)
                                                              CATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATG                                                                                         >  SRR19973062.1953051/1‑150 (MQ=60)
                                                              CATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATG                                                                                         <  SRR19973062.3285785/150‑1 (MQ=60)
                                                              CATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATG                                                                                         <  SRR19973062.3285659/150‑1 (MQ=60)
                                                                 ATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAATCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCAGATGACT                                                                                      >  SRR19973062.3124899/1‑150 (MQ=60)
                                                                   CCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGA                                                                                    <  SRR19973062.3000576/150‑1 (MQ=60)
                                                                     AGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATC                                                                                  <  SRR19973062.4681488/150‑1 (MQ=60)
                                                                     AGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATC                                                                                  <  SRR19973062.481226/150‑1 (MQ=60)
                                                                     AGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATC                                                                                  <  SRR19973062.2124485/150‑1 (MQ=60)
                                                                      GCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCA                                                                                 <  SRR19973062.1994265/150‑1 (MQ=60)
                                                                      GCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCA                                                                                 <  SRR19973062.1993958/150‑1 (MQ=60)
                                                                      GCGCAGTTTTTACTTTTGGTGCCGGATTTGCCTTATCCATCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCTCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCA                                                                                 <  SRR19973062.3279948/150‑1 (MQ=60)
                                                                          AGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCA                                                                             <  SRR19973062.1846778/150‑1 (MQ=60)
                                                                           GTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCAC                                                                            <  SRR19973062.3070603/150‑1 (MQ=60)
                                                                              TTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCC                                                                         >  SRR19973062.1643669/1‑150 (MQ=60)
                                                                              TTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCC                                                                         >  SRR19973062.452760/1‑150 (MQ=60)
                                                                               TTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCA                                                                        <  SRR19973062.888101/150‑1 (MQ=60)
                                                                                  CTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACA                                                                     >  SRR19973062.420742/1‑150 (MQ=60)
                                                                                     TTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATA                                                                  >  SRR19973062.2437899/1‑150 (MQ=60)
                                                                                      TGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATAC                                                                 >  SRR19973062.5121898/1‑150 (MQ=60)
                                                                                      TGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATAC                                                                 <  SRR19973062.5115840/150‑1 (MQ=60)
                                                                                         TGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTG                                                              >  SRR19973062.1738525/1‑150 (MQ=60)
                                                                                          GCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGT                                                             <  SRR19973062.2132995/150‑1 (MQ=60)
                                                                                          GCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGT                                                             <  SRR19973062.4210077/150‑1 (MQ=60)
                                                                                          GCCGGATATGCCTTATCCAGCATAGCTAAGTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGT                                                             <  SRR19973062.4393924/150‑1 (MQ=60)
                                                                                           CCGGATATTCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCACGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTG                                                            <  SRR19973062.1474498/150‑1 (MQ=60)
                                                                                           CCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTG                                                            <  SRR19973062.5192143/150‑1 (MQ=60)
                                                                                              GATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATGCTTGTGCCG                                                         >  SRR19973062.634333/1‑150 (MQ=60)
                                                                                              GATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATGCTTGTGCCG                                                         >  SRR19973062.634309/1‑150 (MQ=60)
                                                                                              GATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCG                                                         >  SRR19973062.2637847/1‑150 (MQ=60)
                                                                                                TATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCG                                                       >  SRR19973062.3789464/1‑150 (MQ=60)
                                                                                                 ATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGC                                                      >  SRR19973062.3980176/1‑150 (MQ=60)
                                                                                                  TGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCT                                                     <  SRR19973062.3517556/150‑1 (MQ=60)
                                                                                                   GCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTG                                                    <  SRR19973062.619688/150‑1 (MQ=60)
                                                                                                     CTTATCCATCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCCCGCACCGCCGCATTTTGTTGGACATGGGCACTCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGAT                                                  <  SRR19973062.3294339/150‑1 (MQ=60)
                                                                                                       TATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATAT                                                <  SRR19973062.3749818/150‑1 (MQ=60)
                                                                                                         TCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTG                                              <  SRR19973062.4608737/150‑1 (MQ=60)
                                                                                                         TCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGATTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTG                                              <  SRR19973062.4235628/150‑1 (MQ=60)
                                                                                                          CCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGT                                             >  SRR19973062.420886/1‑150 (MQ=60)
                                                                                                          CCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGT                                             <  SRR19973062.3040572/150‑1 (MQ=60)
                                                                                                            AGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGA                                           <  SRR19973062.2156872/150‑1 (MQ=60)
                                                                                                              CATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGC                                         >  SRR19973062.702143/1‑150 (MQ=60)
                                                                                                                TAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCAT                                       >  SRR19973062.573640/1‑150 (MQ=60)
                                                                                                                 AGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCAGATGACTGATCACCCACGCCAACAAAACTTGTGCCGCGCTGATATTGTGAGCAAG                                      >  SRR19973062.5006368/1‑150 (MQ=60)
                                                                                                                   CTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTG                                    <  SRR19973062.4787653/150‑1 (MQ=60)
                                                                                                                   CTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTG                                    <  SRR19973062.4787634/150‑1 (MQ=60)
                                                                                                                    TAATTCCGCTGAAGAACGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGC                                   >  SRR19973062.4290631/1‑150 (MQ=60)
                                                                                                                    TAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGC                                   >  SRR19973062.4598556/1‑150 (MQ=60)
                                                                                                                     AATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCA                                  <  SRR19973062.2914163/150‑1 (MQ=60)
                                                                                                                     AATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCA                                  >  SRR19973062.295997/1‑150 (MQ=60)
                                                                                                                      ATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAA                                 >  SRR19973062.4264914/1‑150 (MQ=60)
                                                                                                                       TTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTTATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAT                                <  SRR19973062.5063210/150‑1 (MQ=60)
                                                                                                                       TTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAT                                <  SRR19973062.2419618/150‑1 (MQ=60)
                                                                                                                       TTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAT                                <  SRR19973062.259430/150‑1 (MQ=60)
                                                                                                                       TTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAT                                <  SRR19973062.2047204/150‑1 (MQ=60)
                                                                                                                        TCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATT                               <  SRR19973062.658926/150‑1 (MQ=60)
                                                                                                                        TCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATT                               >  SRR19973062.4478900/1‑150 (MQ=60)
                                                                                                                        TCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATT                               >  SRR19973062.4478603/1‑150 (MQ=60)
                                                                                                                         CCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTTGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTT                              <  SRR19973062.1279652/150‑1 (MQ=60)
                                                                                                                         CCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTT                              <  SRR19973062.1761550/150‑1 (MQ=60)
                                                                                                                           GCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCG                            <  SRR19973062.2082792/150‑1 (MQ=60)
                                                                                                                             TGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATAGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTT                          <  SRR19973062.2086576/150‑1 (MQ=60)
                                                                                                                                AGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGAC                       <  SRR19973062.1742063/150‑1 (MQ=60)
                                                                                                                                AGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGAC                       >  SRR19973062.1493204/1‑150 (MQ=60)
                                                                                                                                AGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGAC                       >  SRR19973062.3550457/1‑150 (MQ=60)
                                                                                                                                AGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGAC                       >  SRR19973062.3701898/1‑150 (MQ=60)
                                                                                                                                AGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGAC                       <  SRR19973062.5233108/150‑1 (MQ=60)
                                                                                                                                AGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCAGATGACTGATCACCCACGCCAACAATAATTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGAC                       >  SRR19973062.1493085/1‑150 (MQ=60)
                                                                                                                                 GAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAATTCGTTGACT                      >  SRR19973062.2367565/1‑150 (MQ=60)
                                                                                                                                 GAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTGTTGGAAACGCCCTCACACCCTGTTGACTGCTCACCCACGCCAACcaaacgtgggccccgcagcgaatgtgagcaagtgcaataacgttgacg                      >  SRR19973062.2114680/1‑102 (MQ=60)
                                                                                                                                   AAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTAC                    >  SRR19973062.5187269/1‑150 (MQ=60)
                                                                                                                                    AGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACC                   <  SRR19973062.1028396/150‑1 (MQ=60)
                                                                                                                                    AGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACC                   <  SRR19973062.5226375/150‑1 (MQ=60)
                                                                                                                                    AGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACC                   <  SRR19973062.4400364/150‑1 (MQ=60)
                                                                                                                                    AGTTCGACCTCAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACC                   <  SRR19973062.3619138/150‑1 (MQ=60)
                                                                                                                                              CAAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTA         <  SRR19973062.2615984/150‑1 (MQ=60)
                                                                                                                                               AAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAA        >  SRR19973062.3730149/1‑150 (MQ=60)
                                                                                                                                               AAGCACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCAGATGACGGATCACCCACGCCAACAATACTTGTGCCGCGCGGATATTGTGAGCATGTGCAATTTCGGTGACTACCGCGGTTTTTAA        >  SRR19973062.2789731/1‑150 (MQ=60)
                                                                                                                                                  CACCGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAG     <  SRR19973062.438316/150‑1 (MQ=60)
                                                                                                                                                     CGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCC  <  SRR19973062.3332191/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCC  >  CP009273/1858983‑1859281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑