Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F56 I0 R1 543 285.0 9366284 95.7% 8963533 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,661,763 C→A 88.7% P353P (CCG→CCT)  mlc ← glucosamine anaerobic growth regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,661,7630CA88.7% 743.0 / 87.9 256P353P (CCG→CCT) mlcglucosamine anaerobic growth regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (15/14);  new base A (99/128);  total (114/142)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 256 total aligned reads displayed.

GAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCA  >  CP009273/1661618‑1661908
                                                                                                                                                 |                                                                                                                                                 
 aaTCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGa                                                                                                                                               >  1:377008/1‑149 (MQ=255)
 aaTCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGa                                                                                                                                               >  2:2844458/1‑149 (MQ=255)
  aTCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGac                                                                                                                                              >  2:619905/1‑148 (MQ=255)
   tCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAag                                                                                                                                             >  1:1228962/1‑149 (MQ=255)
   tCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAag                                                                                                                                             <  2:2088813/149‑1 (MQ=255)
    caacaaAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAaga                                                                                                                                            <  1:282718/149‑1 (MQ=255)
       cacaGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGa                                                                                                                                         <  2:467211/146‑1 (MQ=255)
          aGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGatat                                                                                                                                      >  1:4159416/1‑149 (MQ=255)
           gAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATc                                                                                                                                     <  1:2258429/149‑1 (MQ=255)
            aaCCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCt                                                                                                                                    >  2:1838744/1‑149 (MQ=255)
            aaCCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCt                                                                                                                                    <  1:1749667/149‑1 (MQ=255)
               cGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGcc                                                                                                                                 >  1:4495201/1‑149 (MQ=255)
                  tataCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCt                                                                                                                              >  2:362985/1‑149 (MQ=255)
                   ataCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCtt                                                                                                                             <  2:3442039/149‑1 (MQ=255)
                    taCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCttt                                                                                                                            <  2:2418286/149‑1 (MQ=255)
                     aCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTa                                                                                                                           <  2:1652488/149‑1 (MQ=255)
                       aTCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACt                                                                                                                         <  2:3862490/149‑1 (MQ=255)
                        tCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCTTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACtt                                                                                                                        >  1:3625771/1‑149 (MQ=255)
                        tCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACtt                                                                                                                        >  1:137065/1‑149 (MQ=255)
                        tCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACtt                                                                                                                        >  2:4203137/1‑149 (MQ=255)
                           cgTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAAc                                                                                                                     <  2:3837643/149‑1 (MQ=255)
                            gTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACg                                                                                                                    <  2:1882854/149‑1 (MQ=255)
                            gTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACg                                                                                                                    >  1:3996648/1‑149 (MQ=255)
                            gTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACg                                                                                                                    >  2:344164/1‑149 (MQ=255)
                             tCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACgg                                                                                                                   >  1:503491/1‑149 (MQ=255)
                               ttttACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTg                                                                                                                 >  2:1895916/1‑149 (MQ=255)
                                   aCCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGcc                                                                                                             <  2:1015278/149‑1 (MQ=255)
                                      aGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAAt                                                                                                          >  1:3016253/1‑149 (MQ=255)
                                      aGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAAt                                                                                                          <  1:3457033/149‑1 (MQ=255)
                                         gCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAg                                                                                                       <  2:1482014/149‑1 (MQ=255)
                                          cAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGt                                                                                                      <  1:4010225/149‑1 (MQ=255)
                                           agagcCTGCCATCGTGCCCTGGTTAGAAAACTGAGTTCTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATTCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTa                                                                                                     <  2:2747889/146‑1 (MQ=255)
                                           aGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTa                                                                                                     <  2:79093/149‑1 (MQ=255)
                                           aGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTa                                                                                                     <  2:4462434/149‑1 (MQ=255)
                                           aGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTGTACTTAACGGTGAGCCAATCAGTa                                                                                                     <  1:2252290/149‑1 (MQ=255)
                                            gcgcCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAt                                                                                                    >  1:2220776/1‑149 (MQ=255)
                                            gcgcCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAt                                                                                                    >  1:896031/1‑149 (MQ=255)
                                             cgcCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAtt                                                                                                   <  1:3527946/149‑1 (MQ=255)
                                                cTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAttttt                                                                                                <  2:3834133/149‑1 (MQ=255)
                                                 tGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAtttttt                                                                                               <  1:20768/149‑1 (MQ=255)
                                                  gCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAttttttt                                                                                              >  2:4050589/1‑148 (MQ=255)
                                                     aTCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTgg                                                                                           <  1:549532/149‑1 (MQ=255)
                                                     aTCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTgg                                                                                           <  2:2096677/149‑1 (MQ=255)
                                                     aTCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTgg                                                                                           <  1:3654255/149‑1 (MQ=255)
                                                      tCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTggg                                                                                          >  1:693279/1‑149 (MQ=255)
                                                        gTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGATGGGCCTGCTGACGGATGCTGTCTGAGATGACCTGGAAGAGGATATCTGCCGCTTTTCTTAACGGTGAGCCAATCAGTATTTTTTGTGGGtt                                                                                        <  1:1473184/149‑1 (MQ=255)
                                                        gTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGtt                                                                                        <  2:2779171/149‑1 (MQ=255)
                                                         tGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTta                                                                                       >  1:1328823/1‑149 (MQ=255)
                                                            ccTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaat                                                                                    <  2:2883843/149‑1 (MQ=255)
                                                             cTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaata                                                                                   <  1:4528920/149‑1 (MQ=255)
                                                             cTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaata                                                                                   <  1:4364420/149‑1 (MQ=255)
                                                             cTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaata                                                                                   >  1:3892926/1‑149 (MQ=255)
                                                             cTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATAAGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGTGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAAACAGTATTTTTTGTGGGTtaaata                                                                                   >  1:2989315/1‑149 (MQ=255)
                                                              tGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaataa                                                                                  >  1:4203584/1‑149 (MQ=255)
                                                                  tAGATAACTGAGTACTCTCAACGCTGATGTGCTTACTATACGCATGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGATTTACTTATCGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTc                                                                              <  1:75199/149‑1 (MQ=255)
                                                                    gAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCAc                                                                            <  2:2246102/149‑1 (MQ=255)
                                                                     aaaaCTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCAcc                                                                           >  2:3890818/1‑149 (MQ=255)
                                                                       aaCTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCat                                                                         >  1:3767119/1‑149 (MQ=255)
                                                                        aCTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCatg                                                                        >  1:429720/1‑149 (MQ=255)
                                                                          tGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCatgat                                                                      >  1:3116973/1‑149 (MQ=255)
                                                                                  ctcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGaa                                                              <  2:3563669/149‑1 (MQ=255)
                                                                                  ctcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGaa                                                              <  2:3678402/149‑1 (MQ=255)
                                                                                   tcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAAt                                                             <  2:4024082/149‑1 (MQ=255)
                                                                                     aaCGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATgc                                                           <  2:2465928/149‑1 (MQ=255)
                                                                                      aCGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATATTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATgcg                                                          >  2:454689/1‑149 (MQ=255)
                                                                                      aCGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATgcg                                                          >  2:1220160/1‑149 (MQ=255)
                                                                                         cTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGccc                                                       <  1:354369/149‑1 (MQ=255)
                                                                                           gATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGa                                                     <  2:3710033/149‑1 (MQ=255)
                                                                                             tgtgCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACa                                                   <  2:2230223/149‑1 (MQ=255)
                                                                                             tgtgCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACa                                                   <  1:106944/149‑1 (MQ=255)
                                                                                               tgCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGTCAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATg                                                 <  1:2014677/149‑1 (MQ=255)
                                                                                                gCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATgc                                                >  2:3183194/1‑149 (MQ=255)
                                                                                                 cTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATgcg                                               >  2:316811/1‑149 (MQ=255)
                                                                                                   gACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATgcgcg                                             >  2:594976/1‑149 (MQ=255)
                                                                                                       ataCGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCa                                         <  2:3258069/149‑1 (MQ=255)
                                                                                                         aCGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCAcc                                       >  2:3804163/1‑149 (MQ=255)
                                                                                                           gCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCAcccc                                     <  1:129463/149‑1 (MQ=255)
                                                                                                              ggAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGt                                  <  1:2586520/149‑1 (MQ=255)
                                                                                                                aaGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATCTCTGCCGCTTTACTTAACGGTGAGCNAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTaa                                <  1:4669093/149‑1 (MQ=255)
                                                                                                                 aGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAat                               >  1:156786/1‑149 (MQ=255)
                                                                                                                 aGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAat                               >  1:1913266/1‑149 (MQ=255)
                                                                                                                   ggCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatga                             <  2:3081459/149‑1 (MQ=255)
                                                                                                                    gCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatgat                            >  2:2159697/1‑149 (MQ=255)
                                                                                                                      ctgctgACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGt                          <  2:4002987/149‑1 (MQ=255)
                                                                                                                       tgctgACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTc                         >  1:1240047/1‑149 (MQ=255)
                                                                                                                        gctgACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCt                        >  2:4667550/1‑149 (MQ=255)
                                                                                                                        gctgACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCt                        <  2:659890/149‑1 (MQ=255)
                                                                                                                           gACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCtttt                     >  2:17/1‑149 (MQ=255)
                                                                                                                           gACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCtttt                     <  2:2327434/149‑1 (MQ=255)
                                                                                                                              ggATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGcc                  <  1:1814110/149‑1 (MQ=255)
                                                                                                                               gATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCa                 >  1:2519371/1‑149 (MQ=255)
                                                                                                                                aTGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATTCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAg                >  1:2746830/1‑149 (MQ=255)
                                                                                                                                 tGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGGGCGCCCACCCCGGTAATGCTGTCGGTTGCCAGt               >  2:2696357/1‑149 (MQ=255)
                                                                                                                                  gCTGTCTGAGATGACAGGGTAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTa              <  1:2928043/149‑1 (MQ=255)
                                                                                                                                  gCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTa              <  2:304161/149‑1 (MQ=255)
                                                                                                                                           gaTGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAATTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATcgccgc     >  1:1008733/1‑149 (MQ=255)
                                                                                                                                            aTGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCcgcg    >  1:4274075/1‑149 (MQ=255)
                                                                                                                                             tGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCgcgc   >  1:2099115/1‑149 (MQ=255)
                                                                                                                                             tGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCgcgc   <  2:1124113/149‑1 (MQ=255)
                                                                                                                                              gACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCa  >  2:1243851/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCA  >  CP009273/1661618‑1661908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATG  >  CP009273/1661614‑1661911
                                                                                                                                                     |                                                                                                                                                    
AGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACA                                                                                                                                                      <  SRR19973085.293207/150‑1 (MQ=60)
 GACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAG                                                                                                                                                     <  SRR19973085.4054826/150‑1 (MQ=60)
 GACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAG                                                                                                                                                     <  SRR19973085.4055212/150‑1 (MQ=60)
  ACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGG                                                                                                                                                    <  SRR19973085.269606/150‑1 (MQ=60)
    GAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGA                                                                                                                                                  >  SRR19973085.378478/1‑150 (MQ=60)
      ATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAG                                                                                                                                                <  SRR19973085.2429715/150‑1 (MQ=60)
      ATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAG                                                                                                                                                >  SRR19973085.1234295/1‑150 (MQ=60)
       TCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGA                                                                                                                                               <  SRR19973085.1074791/150‑1 (MQ=60)
        CAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAG                                                                                                                                              <  SRR19973085.283970/150‑1 (MQ=60)
           AAAAGAACAGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTTGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGATGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGTAGAGGAT                                                                                                                                           <  SRR19973085.2946180/150‑1 (MQ=60)
             AAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGACGAGGATAT                                                                                                                                         >  SRR19973085.4176740/1‑150 (MQ=60)
             AAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATAT                                                                                                                                         >  SRR19973085.2026925/1‑150 (MQ=60)
               GAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCT                                                                                                                                       >  SRR19973085.1308832/1‑150 (MQ=60)
               GAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCT                                                                                                                                       <  SRR19973085.2268193/150‑1 (MQ=60)
                AACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTG                                                                                                                                      >  SRR19973085.1927486/1‑150 (MQ=60)
                AACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTG                                                                                                                                      <  SRR19973085.1757299/150‑1 (MQ=60)
                  CCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCC                                                                                                                                    >  SRR19973085.4513908/1‑150 (MQ=60)
                   CGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCG                                                                                                                                   >  SRR19973085.4007779/1‑150 (MQ=60)
                   CGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCG                                                                                                                                   <  SRR19973085.2585544/150‑1 (MQ=60)
                   CGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCG                                                                                                                                   <  SRR19973085.2263901/150‑1 (MQ=60)
                   CGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCG                                                                                                                                   <  SRR19973085.4614884/150‑1 (MQ=60)
                    GTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGC                                                                                                                                  >  SRR19973085.4293018/1‑150 (MQ=60)
                     TTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCT                                                                                                                                 >  SRR19973085.4102093/1‑150 (MQ=60)
                     TTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCT                                                                                                                                 >  SRR19973085.3077216/1‑150 (MQ=60)
                       ATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTT                                                                                                                               >  SRR19973085.742823/1‑150 (MQ=60)
                       ATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTT                                                                                                                               >  SRR19973085.662853/1‑150 (MQ=60)
                       ATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTT                                                                                                                               >  SRR19973085.3580771/1‑150 (MQ=60)
                        TACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTA                                                                                                                              >  SRR19973085.4361000/1‑150 (MQ=60)
                        TACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTA                                                                                                                              >  SRR19973085.4361018/1‑150 (MQ=60)
                           ATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCTTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTT                                                                                                                           >  SRR19973085.3640887/1‑150 (MQ=60)
                           ATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTT                                                                                                                           >  SRR19973085.137832/1‑150 (MQ=60)
                           ATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTT                                                                                                                           <  SRR19973085.3376185/150‑1 (MQ=60)
                               CGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACG                                                                                                                       >  SRR19973085.2824733/1‑150 (MQ=60)
                               CGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACG                                                                                                                       >  SRR19973085.963165/1‑150 (MQ=60)
                               CGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACG                                                                                                                       >  SRR19973085.4013340/1‑150 (MQ=60)
                                GTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGG                                                                                                                      >  SRR19973085.505726/1‑150 (MQ=60)
                                GTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGG                                                                                                                      >  SRR19973085.505830/1‑150 (MQ=60)
                                GTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGG                                                                                                                      >  SRR19973085.506237/1‑150 (MQ=60)
                                      TACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCC                                                                                                                <  SRR19973085.2250458/150‑1 (MQ=60)
                                       ACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCA                                                                                                               <  SRR19973085.3514905/150‑1 (MQ=60)
                                       ACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCA                                                                                                               <  SRR19973085.4023533/150‑1 (MQ=60)
                                         CAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAAT                                                                                                             <  SRR19973085.1000889/150‑1 (MQ=60)
                                         CAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAAT                                                                                                             >  SRR19973085.3028928/1‑150 (MQ=60)
                                         CAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAAT                                                                                                             <  SRR19973085.2413211/150‑1 (MQ=60)
                                          AGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATC                                                                                                            <  SRR19973085.3558337/150‑1 (MQ=60)
                                          AGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATC                                                                                                            <  SRR19973085.3558185/150‑1 (MQ=60)
                                          AGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATC                                                                                                            <  SRR19973085.3471314/150‑1 (MQ=60)
                                              CAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTA                                                                                                        <  SRR19973085.4026988/150‑1 (MQ=60)
                                               AGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAT                                                                                                       >  SRR19973085.2230430/1‑150 (MQ=60)
                                               AGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAT                                                                                                       >  SRR19973085.900033/1‑150 (MQ=60)
                                               AGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAT                                                                                                       <  SRR19973085.4612750/150‑1 (MQ=60)
                                               AGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTGTACTTAACGGTGAGCCAATCAGTAT                                                                                                       <  SRR19973085.2262039/150‑1 (MQ=60)
                                                 CGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTT                                                                                                     <  SRR19973085.3542461/150‑1 (MQ=60)
                                                  GCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTCCTCTCAACGCTGATGTGCTGACTATCCGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTT                                                                                                    <  SRR19973085.1072113/150‑1 (MQ=60)
                                                  GCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTT                                                                                                    >  SRR19973085.3077821/1‑150 (MQ=60)
                                                     TGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTG                                                                                                 <  SRR19973085.1295380/150‑1 (MQ=60)
                                                     TGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTG                                                                                                 <  SRR19973085.1295387/150‑1 (MQ=60)
                                                     TGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTG                                                                                                 <  SRR19973085.20955/150‑1 (MQ=60)
                                                      GCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGT                                                                                                <  SRR19973085.2174218/150‑1 (MQ=60)
                                                      GCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGT                                                                                                <  SRR19973085.3471481/150‑1 (MQ=60)
                                                         ATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGG                                                                                             <  SRR19973085.552071/150‑1 (MQ=60)
                                                         ATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGG                                                                                             >  SRR19973085.696346/1‑150 (MQ=60)
                                                         ATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGG                                                                                             <  SRR19973085.3669513/150‑1 (MQ=60)
                                                           CGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTT                                                                                           >  SRR19973085.4259206/1‑150 (MQ=60)
                                                           CGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTT                                                                                           >  SRR19973085.1916651/1‑150 (MQ=60)
                                                           CGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTT                                                                                           <  SRR19973085.1379502/150‑1 (MQ=60)
                                                           CGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTT                                                                                           <  SRR19973085.4515839/150‑1 (MQ=60)
                                                           CGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTT                                                                                           <  SRR19973085.4515882/150‑1 (MQ=60)
                                                            GTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGATGGGCCTGCTGACGGATGCTGTCTGAGATGACCTGGAAGAGGATATCTGCCGCTTTTCTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTA                                                                                          <  SRR19973085.1479555/150‑1 (MQ=60)
                                                            GTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTA                                                                                          <  SRR19973085.2705232/150‑1 (MQ=60)
                                                            GTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTA                                                                                          >  SRR19973085.1334687/1‑150 (MQ=60)
                                                              atacgagatggctgagttagtgactggagttcagacgtgtgctcttccgatctGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAA                                                                                        <  SRR19973085.1100848/97‑1 (MQ=60)
                                                                CCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATA                                                                                      <  SRR19973085.3749980/150‑1 (MQ=60)
                                                                CCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATA                                                                                      >  SRR19973085.3908962/1‑150 (MQ=60)
                                                                CCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATAAGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGTGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAAACAGTATTTTTTGTGGGTTAAATA                                                                                      >  SRR19973085.3001918/1‑150 (MQ=60)
                                                                 CTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAA                                                                                     >  SRR19973085.4221032/1‑150 (MQ=60)
                                                                 CTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAA                                                                                     <  SRR19973085.4382505/150‑1 (MQ=60)
                                                                 CTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAA                                                                                     <  SRR19973085.4547770/150‑1 (MQ=60)
                                                                      TAGATAACTGAGTACTCTCAACGCTGATGTGCTTACTATACGCATGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGATTTACTTATCGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCA                                                                                <  SRR19973085.75683/150‑1 (MQ=60)
                                                                       AGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCAC                                                                               <  SRR19973085.3904976/150‑1 (MQ=60)
                                                                        GAATACTGAGTACTCTCAACGCTGATGTGCTGACTATTCGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAAGTCACC                                                                              <  SRR19973085.4291297/150‑1 (MQ=60)
                                                                        GAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACC                                                                              <  SRR19973085.1593216/150‑1 (MQ=60)
                                                                          AAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCAT                                                                            >  SRR19973085.3782787/1‑150 (MQ=60)
                                                                          AAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCAT                                                                            >  SRR19973085.3782803/1‑150 (MQ=60)
                                                                           AACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATG                                                                           >  SRR19973085.431589/1‑150 (MQ=60)
                                                                             CTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGAT                                                                         >  SRR19973085.3129865/1‑150 (MQ=60)
                                                                                      CTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAAT                                                                >  SRR19973085.845591/1‑150 (MQ=60)
                                                                                         AACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCG                                                             >  SRR19973085.21247/1‑150 (MQ=60)
                                                                                            GCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCC                                                          >  SRR19973085.1797457/1‑150 (MQ=60)
                                                                                             CTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCG                                                         <  SRR19973085.355794/150‑1 (MQ=60)
                                                                                                 TGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACAT                                                     >  SRR19973085.4085669/1‑150 (MQ=60)
                                                                                                 TGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACAT                                                     <  SRR19973085.107598/150‑1 (MQ=60)
                                                                                                   TGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGTCAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGC                                                   <  SRR19973085.2023358/150‑1 (MQ=60)
                                                                                                               gtggtgacgataTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCG                                       <  SRR19973085.1372437/138‑1 (MQ=60)
                                                                                                               GCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCG                                       <  SRR19973085.130214/150‑1 (MQ=60)
                                                                                                                  GGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTA                                    <  SRR19973085.2597585/150‑1 (MQ=60)
                                                                                                                  GGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTA                                    >  SRR19973085.700934/1‑150 (MQ=60)
                                                                                                                    AAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAAT                                  >  SRR19973085.157632/1‑150 (MQ=60)
                                                                                                                    AAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATCTCTGCCGCTTTACTTAACGGTGAGCNAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAAT                                  <  SRR19973085.4688364/150‑1 (MQ=60)
                                                                                                                    AAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAAT                                  >  SRR19973085.1921428/1‑150 (MQ=60)
                                                                                                                    AAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAAT                                  >  SRR19973085.1920844/1‑150 (MQ=60)
                                                                                                                     AGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATG                                 <  SRR19973085.364428/150‑1 (MQ=60)
                                                                                                                      GGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGA                                >  SRR19973085.3697337/1‑150 (MQ=60)
                                                                                                                       GGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGAT                               <  SRR19973085.1673330/150‑1 (MQ=60)
                                                                                                                          CTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTC                            >  SRR19973085.1245456/1‑150 (MQ=60)
                                                                                                                           TGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCT                           <  SRR19973085.1364126/150‑1 (MQ=60)
                                                                                                                           TGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCT                           <  SRR19973085.1364182/150‑1 (MQ=60)
                                                                                                                             CTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTT                         >  SRR19973085.3014713/1‑150 (MQ=60)
                                                                                                                             CTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTT                         >  SRR19973085.3015114/1‑150 (MQ=60)
                                                                                                                              TGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTT                        <  SRR19973085.4206360/150‑1 (MQ=60)
                                                                                                                                  GGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCA                    <  SRR19973085.1822015/150‑1 (MQ=60)
                                                                                                                                  GGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCA                    >  SRR19973085.2530198/1‑150 (MQ=60)
                                                                                                                                   GATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATTCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAG                   >  SRR19973085.2758344/1‑150 (MQ=60)
                                                                                                                                   GATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAG                   >  SRR19973085.2046523/1‑150 (MQ=60)
                                                                                                                                    ATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGT                  <  SRR19973085.2266952/150‑1 (MQ=60)
                                                                                                                                     TGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTA                 <  SRR19973085.2748601/150‑1 (MQ=60)
                                                                                                                                      GCTGTCTGAGATGACAGGGTAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAG                <  SRR19973085.2940470/150‑1 (MQ=60)
                                                                                                                                         GTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATC             <  SRR19973085.515283/150‑1 (MQ=60)
                                                                                                                                           CTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGC           >  SRR19973085.1520933/1‑150 (MQ=60)
                                                                                                                                           CTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGC           >  SRR19973085.124368/1‑150 (MQ=60)
                                                                                                                                           CTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGC           >  SRR19973085.1520957/1‑150 (MQ=60)
                                                                                                                                           CTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGC           >  SRR19973085.3867844/1‑150 (MQ=60)
                                                                                                                                           CTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAAAGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAAATCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGC           >  SRR19973085.3944405/1‑150 (MQ=60)
                                                                                                                                            TGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCC          >  SRR19973085.2937063/1‑150 (MQ=60)
                                                                                                                                            TGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCC          >  SRR19973085.2937419/1‑150 (MQ=60)
                                                                                                                                              AGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGC        >  SRR19973085.439216/1‑150 (MQ=60)
                                                                                                                                              AGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAATTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGC        >  SRR19973085.1013204/1‑150 (MQ=60)
                                                                                                                                               GATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCG       >  SRR19973085.4291665/1‑150 (MQ=60)
                                                                                                                                                ATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGC      >  SRR19973085.2108188/1‑150 (MQ=60)
                                                                                                                                                ATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGC      >  SRR19973085.2108228/1‑150 (MQ=60)
                                                                                                                                                   ACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAAT   <  SRR19973085.459482/150‑1 (MQ=60)
                                                                                                                                                   ACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCACACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAAT   <  SRR19973085.918100/150‑1 (MQ=60)
                                                                                                                                                    CAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATG  >  SRR19973085.115913/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATG  >  CP009273/1661614‑1661911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑