Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,669,592 G→T 100% F342L (TTC→TTA)  pntB ← pyridine nucleotide transhydrogenase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,669,5920GT97.3% 687.2 / 7.7 219F342L (TTC→TTA) pntBpyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (1/5);  new base T (116/97);  total (117/102)
Fisher's exact test for biased strand distribution p-value = 9.94e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 219 total aligned reads displayed.

TAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGA  >  CP009273/1669447‑1669737
                                                                                                                                                  |                                                                                                                                                 
tAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTaaa                                                                                                                                                <  1:3765168/149‑1 (MQ=255)
    aCCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGca                                                                                                                                            >  2:2893180/1‑149 (MQ=255)
    aCCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGca                                                                                                                                            >  2:622624/1‑149 (MQ=255)
        aTCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACAtt                                                                                                                                        <  2:2027376/149‑1 (MQ=255)
         tCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTa                                                                                                                                       >  2:3884613/1‑149 (MQ=255)
          cacatgTAGGGTTTCTGTATCTGCAAAGTAATAATTGATCTCGACCTTTTCCAGCACGTTGTAATAGGGTACTTTTGCTTCAGCCAGAATTAGGTTCATTTGCCCAGGAAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTaa                                                                                                                                      <  1:3604317/144‑1 (MQ=255)
          cACCAGTCCGGTATCGTTATCAGAAATGTCATCATTGATATCGTCCATTTCCACCACGTTGTAATACGGTACTTTTGCTTCCGCCAGAAATACTTTAATATGTCCAGGAAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTaa                                                                                                                                      <  1:1556023/149‑1 (MQ=255)
          cACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTaa                                                                                                                                      <  2:2595640/149‑1 (MQ=255)
              aGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATAcc                                                                                                                                  >  2:843698/1‑149 (MQ=255)
               gTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCa                                                                                                                                 >  1:2677334/1‑149 (MQ=255)
                   ggtatcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAg                                                                                                                             <  2:2486175/149‑1 (MQ=255)
                    gtatcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgc                                                                                                                            <  2:3405266/149‑1 (MQ=255)
                    gtatcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgc                                                                                                                            <  2:3405286/149‑1 (MQ=255)
                     tatcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgcg                                                                                                                           >  2:2126859/1‑149 (MQ=255)
                     tatcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgcg                                                                                                                           >  1:1581176/1‑149 (MQ=255)
                     tatcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgag                                                                                                                           >  2:2332914/1‑147 (MQ=255)
                       tcgttatcAGAAATGTAATATTTGATCTGGTCCATTTCCCGCACTTTGTAATACGTTACTTTTGCTTCAGCCAGCAATACGTTCATATGGCCAGGAAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgcgcg                                                                                                                         <  1:1112699/149‑1 (MQ=255)
                             ncAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAttt                                                                                                                   >  1:3519337/2‑149 (MQ=255)
                              cAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTtc                                                                                                                  >  2:544031/1‑149 (MQ=255)
                               aGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTtct                                                                                                                 >  2:748241/1‑149 (MQ=255)
                                     gTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTaa                                                                                                           <  2:3031993/149‑1 (MQ=255)
                                     gTCATCATTGATCTCGTCAATTTCCAGCACGATGTCATAGGTTACTTTTGCTTCAGCCAGCAATACGTTAATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTaa                                                                                                           <  1:2909246/149‑1 (MQ=255)
                                      tcatcaTTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAt                                                                                                          >  1:3844281/1‑149 (MQ=255)
                                       catcatTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAtg                                                                                                         <  2:3844022/149‑2 (MQ=255)
                                        atcatTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAttt                                                                                                        >  1:4087691/1‑149 (MQ=255)
                                         tcatTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTc                                                                                                       <  2:64474/149‑1 (MQ=255)
                                          catTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCa                                                                                                      <  1:1675610/149‑1 (MQ=255)
                                           atTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCACA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAg                                                                                                     >  1:3994861/1‑149 (MQ=255)
                                           atTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAg                                                                                                     >  1:1017942/1‑149 (MQ=255)
                                            ttGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGc                                                                                                    >  2:1162208/1‑149 (MQ=255)
                                             tGATCTCGTCCATTTCCAGCACGATGTCATACGGTAATTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCg                                                                                                   <  1:3234623/149‑1 (MQ=255)
                                               aTCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAc                                                                                                 >  1:3784479/1‑149 (MQ=255)
                                               aTCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAc                                                                                                 >  1:3818959/1‑149 (MQ=255)
                                               aTCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAc                                                                                                 >  2:3678484/1‑149 (MQ=255)
                                               aTCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAc                                                                                                 <  2:1219444/149‑1 (MQ=255)
                                                tctcGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACa                                                                                                <  2:843156/149‑1 (MQ=255)
                                                tctcGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACa                                                                                                >  2:1092007/1‑149 (MQ=255)
                                                   cGTCCTTTTCCAGCACTTTGTAATAGGGTATTTTTGATTCAGCCAGAAATCCTTTAATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGAACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGa                                                                                             <  1:3679883/149‑1 (MQ=255)
                                                   cGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGa                                                                                             >  1:1172094/1‑149 (MQ=255)
                                                      ccATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGatat                                                                                          >  2:3595703/1‑149 (MQ=255)
                                                      ccATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGatat                                                                                          >  2:1549367/1‑149 (MQ=255)
                                                        aTTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTg                                                                                        >  2:2459216/1‑149 (MQ=255)
                                                           tCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGAAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgcgc                                                                                     <  1:2734687/149‑1 (MQ=255)
                                                           tCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTAATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgcgc                                                                                     <  1:2733653/149‑1 (MQ=255)
                                                             cAGCACGATGTCATAGGTTACTTTTGCTTCAGCCAGAAATACGTTAATATGTCCCGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCt                                                                                   <  1:1995692/149‑1 (MQ=255)
                                                                    aTGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGAc                                                                            <  2:3349741/149‑1 (MQ=255)
                                                                    aTGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGAc                                                                            <  2:1613247/149‑1 (MQ=255)
                                                                     tGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACt                                                                           >  1:1151975/1‑149 (MQ=255)
                                                                     tGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACt                                                                           >  1:3330330/1‑149 (MQ=255)
                                                                     tGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACt                                                                           >  2:4012366/1‑149 (MQ=255)
                                                                       tCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGc                                                                         >  1:1601476/1‑149 (MQ=255)
                                                                         aTACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCCGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCa                                                                       <  2:1896719/149‑1 (MQ=255)
                                                                            cgttagttttGCTTCAGCCAGCAATCCGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTGCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGc                                                                    <  2:1430502/143‑1 (MQ=255)
                                                                              gTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCg                                                                  >  2:311244/1‑149 (MQ=255)
                                                                              gTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCg                                                                  >  1:3628207/1‑149 (MQ=255)
                                                                                  ttttGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAcc                                                              >  1:395869/1‑149 (MQ=255)
                                                                                    ttGATTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAcccc                                                            <  1:4112102/149‑1 (MQ=255)
                                                                                       cTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGa                                                         >  2:3744558/1‑149 (MQ=255)
                                                                                        ttCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAg                                                        >  2:3295242/1‑149 (MQ=255)
                                                                                         tCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGt                                                       <  2:550623/149‑1 (MQ=255)
                                                                                           agccagcAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTaa                                                     >  2:706220/1‑149 (MQ=255)
                                                                                           agccagcAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTaa                                                     >  1:477708/1‑149 (MQ=255)
                                                                                            gccagcAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGTGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAt                                                    >  1:3128591/1‑149 (MQ=255)
                                                                                            gccagcAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAt                                                    >  1:1815719/1‑149 (MQ=255)
                                                                                            gccagcAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAt                                                    <  2:2093572/149‑1 (MQ=255)
                                                                                              cagcAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGa                                                  >  1:583147/1‑149 (MQ=255)
                                                                                                gcAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATc                                                >  2:2384905/1‑149 (MQ=255)
                                                                                                  aaTACTTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCAc                                              <  1:1708769/149‑1 (MQ=255)
                                                                                                  aaTACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCAc                                              <  2:177940/149‑1 (MQ=255)
                                                                                                  aaTACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCAc                                              <  2:1420193/149‑1 (MQ=255)
                                                                                                    tACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTg                                            >  2:3661976/1‑149 (MQ=255)
                                                                                                         tCATATGTCCAGGCAAC‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATgg                                       <  2:3143119/149‑1 (MQ=255)
                                                                                                         tCATATGACCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCCGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATgg                                       <  2:3143943/149‑1 (MQ=255)
                                                                                                           atatGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGa                                     >  2:3858874/1‑149 (MQ=255)
                                                                                                            tatGTCCAGGCAAA‑CGCCCCCCTACGGGTTGGATACCTAAACGAAATTTAATACCACGAGCGCTAATTTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAg                                    <  2:397785/149‑1 (MQ=255)
                                                                                                              tGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtt                                  >  2:46950/1‑149 (MQ=255)
                                                                                                               gTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGttt                                 <  2:3217020/149‑1 (MQ=255)
                                                                                                                tCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtttt                                >  2:2528569/1‑149 (MQ=255)
                                                                                                                 ccAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGttttt                               <  2:3200832/149‑1 (MQ=255)
                                                                                                                 ccAGGCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGttttt                               >  2:3342974/1‑149 (MQ=255)
                                                                                                                   cagCAAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATCCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTc                              <  1:1095062/147‑1 (MQ=255)
                                                                                                                    ggCAAC‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcag                            <  1:847300/149‑1 (MQ=255)
                                                                                                                     gCAAA‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcagc                           >  2:3785927/1‑149 (MQ=255)
                                                                                                                       aaa‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcagcag                         >  1:3368069/1‑149 (MQ=255)
                                                                                                                        aa‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGt                        >  1:947309/1‑149 (MQ=255)
                                                                                                                        aa‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGt                        >  2:2745093/1‑149 (MQ=255)
                                                                                                                        aa‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTAAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGt                        <  1:2332914/149‑1 (MQ=255)
                                                                                                                        aa‑CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAGTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGt                        >  1:3005390/1‑149 (MQ=255)
                                                                                                                            gCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTcc                     <  1:677116/149‑1 (MQ=255)
                                                                                                                            gCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTcc                     <  1:2126906/149‑1 (MQ=255)
                                                                                                                              cccGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGc                   >  1:1375733/1‑149 (MQ=255)
                                                                                                                               ccGCGACCGGGTGGATACCTAAACCCACATTAATACCACGAGCGCGCAATGTCACAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCt                  <  1:2557674/149‑1 (MQ=255)
                                                                                                                                cgcgACCGGGTGGATACCTAAACGCACATTAATACCCCGAGCGCGCAATTTCACCGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTg                 <  1:1945143/149‑1 (MQ=255)
                                                                                                                                   gACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGtct              >  2:4040768/1‑149 (MQ=255)
                                                                                                                                   gACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGtct              >  2:4040748/1‑149 (MQ=255)
                                                                                                                                    aCCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGtctc             >  1:2823018/1‑149 (MQ=255)
                                                                                                                                     ccGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCAGCTGTctct            <  1:868415/149‑1 (MQ=255)
                                                                                                                                           ggATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGACTGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCg      >  2:3547175/1‑149 (MQ=255)
                                                                                                                                            gATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCgg     >  2:438271/1‑149 (MQ=255)
                                                                                                                                               aCCTAAACGCACATTTATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGa  <  2:3519337/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAA‑CGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGA  >  CP009273/1669447‑1669737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTT  >  CP009273/1669444‑1669740
                                                                                                                                                    |                                                                                                                                                    
CGTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTA                                                                                                                                                     >  SRR19973068.1213304/1‑150 (MQ=60)
CGTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTA                                                                                                                                                     >  SRR19973068.3898736/1‑150 (MQ=60)
CGTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTA                                                                                                                                                     >  SRR19973068.1213024/1‑150 (MQ=60)
   TAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAAC                                                                                                                                                  <  SRR19973068.3787654/150‑1 (MQ=60)
      CACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCA                                                                                                                                               >  SRR19973068.1213740/1‑150 (MQ=60)
       ACAAATCACCAGTACGGTATCGGTATCAGAAAAGTCATCATTGATCTCGTCAATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGAAATACGTTCATTTGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCAC                                                                                                                                              <  SRR19973068.2542320/150‑1 (MQ=60)
        CCAATCACCAGTACGGTATCGGTATCAACAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACA                                                                                                                                             >  SRR19973068.2621540/1‑150 (MQ=60)
             cacatgtagggtttctGTATCTGCAAAGTAATAATTGATCTCGACCTTTTCCAGCACGTTGTAATAGGGTACTTTTGCTTCAGCCAGAATTAGGTTCATTTGCCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAAT                                                                                                                                        <  SRR19973068.3626202/134‑1 (MQ=56)
             CACCAGTCCGGTATCGTTATCAGAAATGTCATCATTGATATCGTCCATTTCCACCACGTTGTAATACGGTACTTTTGCTTCCGCCAGAAATACTTTAATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAAT                                                                                                                                        <  SRR19973068.1566263/150‑1 (MQ=60)
             CACCAGTACGTTATCGGTATCAGAAAAGTCATCATTGATCTCGGCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAAT                                                                                                                                        <  SRR19973068.1566313/150‑1 (MQ=60)
               CCAGTACGGTATCGGTATCAGAAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGTTACTTTTGCTTCAGCCAGAAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATAC                                                                                                                                      <  SRR19973068.1986624/150‑1 (MQ=60)
                 AGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCA                                                                                                                                    >  SRR19973068.2694025/1‑150 (MQ=60)
                 AGTACGGTATCGGTATCAGCAAAGTCATCAATGATCTCGTCCATTTCCAGCACGATTTCATACTGTACTTATTCTTCAGACACCAATAATTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACAaaaatacaa                                                                                                                                    >  SRR19973068.400566/1‑141 (MQ=60)
                  GTACGGTATCGGTATCAGAAAAGTAATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGAAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCAC                                                                                                                                   <  SRR19973068.1862539/150‑1 (MQ=60)
                   tacgttatggttatcataaatgtaATCATTGATCTCGTAAATTTCCAGCAAGATGTATTCCGTTAATTTTGGTTCTGCCAGCAATATGTTATTATGTCCAGGCAAAGGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACG                                                                                                                                  <  SRR19973068.2911183/126‑1 (MQ=45)
                   TACGGTATCGTTATAAGAAAAGTAATCTTTGATCTCGTCCATTTCCAGCACGATGTAATAGGGTAATTTTGCTTCAGCCAGCAATACGTTCATATGTCCTGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACG                                                                                                                                  <  SRR19973068.2489868/150‑1 (MQ=60)
                       GTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCG                                                                                                                              >  SRR19973068.1591566/1‑150 (MQ=60)
                          tcgttatcagaaatgtaatatTTGATCTGGTCCATTTCCCGCACTTTGTAATACGTTACTTTTGCTTCAGCCAGCAATACGTTCATATGGCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGC                                                                                                                           <  SRR19973068.1120128/129‑1 (MQ=60)
                          TCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGTTGTCATAGGGTAATTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGC                                                                                                                           <  SRR19973068.1120050/150‑1 (MQ=60)
                           CGGTATCAGCAAAGTCATCATTTATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATCCGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCA                                                                                                                          <  SRR19973068.3258167/150‑1 (MQ=60)
                             GTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAT                                                                                                                        >  SRR19973068.3469060/1‑150 (MQ=60)
                               ATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTT                                                                                                                      <  SRR19973068.3649164/150‑1 (MQ=60)
                               ANCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTT                                                                                                                      >  SRR19973068.3540741/1‑150 (MQ=60)
                                        GTCATCATTGATCTCGTCCTTTTCCAGCACGTTTTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAT                                                                                                             <  SRR19973068.855167/150‑1 (MQ=60)
                                        GTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAT                                                                                                             >  SRR19973068.3867476/1‑150 (MQ=60)
                                        GTCATCATTGATCTCGTCAATTTCCAGCACGATGTCATAGGTTACTTTTGCTTCAGCCAGCAATACGTTAATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAT                                                                                                             <  SRR19973068.2927342/150‑1 (MQ=60)
                                         TCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATT                                                                                                            >  SRR19973068.2729081/1‑150 (MQ=60)
                                          CATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTT                                                                                                           >  SRR19973068.4112201/1‑150 (MQ=60)
                                          CATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTT                                                                                                           >  SRR19973068.4126339/1‑150 (MQ=60)
                                            TCATTGATCTCGTCCATTTCCAGCACGATGTCTTACGGTACTTTTGCTTCAGCCAGCAATCCGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCA                                                                                                         <  SRR19973068.1607920/150‑1 (MQ=60)
                                            TCATTGATCTCGTCCATTTCCAGCACGATGTCATAGGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCA                                                                                                         <  SRR19973068.3736387/150‑1 (MQ=60)
                                            TCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCA                                                                                                         <  SRR19973068.3439132/150‑1 (MQ=60)
                                             CATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCACACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAG                                                                                                        >  SRR19973068.4019009/1‑150 (MQ=60)
                                             CATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAG                                                                                                        >  SRR19973068.4019484/1‑150 (MQ=60)
                                             CATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAG                                                                                                        >  SRR19973068.1024553/1‑150 (MQ=60)
                                             CATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAG                                                                                                        >  SRR19973068.754132/1‑150 (MQ=60)
                                             CATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAG                                                                                                        <  SRR19973068.1686406/150‑1 (MQ=60)
                                             CATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAG                                                                                                        <  SRR19973068.1686459/150‑1 (MQ=60)
                                                TGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGA                                                                                                     >  SRR19973068.3567077/1‑150 (MQ=60)
                                                TGATCTCGTCCATTTCCAGCACGATGTCATACGGTAATTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGA                                                                                                     <  SRR19973068.3254505/150‑1 (MQ=60)
                                                 tctctggtcatttgcccgcacgttttctttgttttttttttcttcagcaagaaatactttattaggtccagcaAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAC                                                                                                    <  SRR19973068.1760673/77‑1 (MQ=60)
                                                 tatctcgtccttttcctgaagtttgtaatacgttatTTTTGCTCCAGCCAGAAATACTTTAATATGCCCAGCCAAACCCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAC                                                                                                    <  SRR19973068.20049/114‑1 (MQ=60)
                                                 GATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAC                                                                                                    >  SRR19973068.3841934/1‑150 (MQ=60)
                                                 GATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAC                                                                                                    >  SRR19973068.3807055/1‑150 (MQ=60)
                                                 GATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAC                                                                                                    >  SRR19973068.3807084/1‑150 (MQ=60)
                                                 GATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAC                                                                                                    >  SRR19973068.1789619/1‑150 (MQ=60)
                                                     TCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGA                                                                                                >  SRR19973068.1179078/1‑150 (MQ=60)
                                                     TCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGA                                                                                                >  SRR19973068.1179929/1‑150 (MQ=60)
                                                      CGTCCTTTTCCAGCACTTTGTAATAGGGTATTTTTGATTCAGCCAGAAATCCTTTAATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGAACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGAT                                                                                               <  SRR19973068.3702099/150‑1 (MQ=60)
                                                       GTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATA                                                                                              >  SRR19973068.3810134/1‑150 (MQ=60)
                                                          CATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTG                                                                                           >  SRR19973068.1641698/1‑150 (MQ=60)
                                                          CATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTG                                                                                           >  SRR19973068.3698269/1‑150 (MQ=60)
                                                           ATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGC                                                                                          >  SRR19973068.2249134/1‑150 (MQ=60)
                                                             TTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGC                                                                                        >  SRR19973068.1777720/1‑150 (MQ=60)
                                                              tcccccactttgtaataggtTACTTTTGCTTCACCCAGAAATACTTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCC                                                                                       <  SRR19973068.1309181/130‑1 (MQ=60)
                                                              TCCAGCACGATGTCATACGTTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCC                                                                                       <  SRR19973068.2751546/150‑1 (MQ=60)
                                                              TCCAGCACGATGTCATACGGTACTTTTGCTTCCGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCC                                                                                       <  SRR19973068.2751538/150‑1 (MQ=60)
                                                              TCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTAATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCC                                                                                       <  SRR19973068.2750508/150‑1 (MQ=60)
                                                               ccagcactttttaatcggttatttttgcttccgccaccattactttaatttgccctgcaaaaccCCCCGCGACCGGGTGTATACCTAAACGCACATTAATCCCACGAGCGCGAAATTTCACCGTAATTTCAGCGACAGGATATTGCGCCT                                                                                      <  SRR19973068.455097/86‑1 (MQ=60)
                                                               ccagcacttttaattcgggtacttttgttccccccagaaatcctttcatatgccctggaaacCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCT                                                                                      <  SRR19973068.2538241/88‑1 (MQ=60)
                                                                CAGCACGATGTCATAGGTTACTTTTGCTTCAGCCAGAAATACGTTAATATGTCCCGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTG                                                                                     <  SRR19973068.2008845/150‑1 (MQ=60)
                                                                       ATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACT                                                                              >  SRR19973068.1159652/1‑150 (MQ=60)
                                                                       ATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACT                                                                              >  SRR19973068.3350655/1‑150 (MQ=60)
                                                                       ATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACT                                                                              >  SRR19973068.2786694/1‑150 (MQ=60)
                                                                       ATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACT                                                                              >  SRR19973068.1159685/1‑150 (MQ=60)
                                                                         GTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGC                                                                            >  SRR19973068.1611975/1‑150 (MQ=60)
                                                                                gtaaaTTTTGCTACAGCCAGCAATACTTTCTTATGGCCAGGAAACCGCCCCGCGACCGGGTGTATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCG                                                                     <  SRR19973068.4035504/145‑1 (MQ=60)
                                                                                GGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCG                                                                     >  SRR19973068.3650206/1‑150 (MQ=60)
                                                                                  TACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTA                                                                   >  SRR19973068.3793057/1‑150 (MQ=60)
                                                                                   ACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAC                                                                  >  SRR19973068.1450624/1‑150 (MQ=60)
                                                                                    CTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACC                                                                 >  SRR19973068.398632/1‑150 (MQ=60)
                                                                                      TTTGCTTCAGCCAGCAATCCGTTCATAGGCCCAGGCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCCCGAGCGCGCAATTTCTCCGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGAACCCC                                                               <  SRR19973068.3852972/150‑1 (MQ=60)
                                                                                       TTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCG                                                              <  SRR19973068.4136866/150‑1 (MQ=60)
                                                                                       TTGATTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCG                                                              <  SRR19973068.4136697/150‑1 (MQ=60)
                                                                                        TGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGG                                                             >  SRR19973068.945526/1‑150 (MQ=60)
                                                                                             CAGCCAGCAATAGTTTCATATGGCCAGGCAACCGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAA                                                        <  SRR19973068.2944117/150‑1 (MQ=60)
                                                                                             CAGCCAGCAATAGGTTAATAGGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAA                                                        <  SRR19973068.2943959/150‑1 (MQ=60)
                                                                                             CAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAA                                                        >  SRR19973068.481080/1‑150 (MQ=60)
                                                                                              AGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGTGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAT                                                       >  SRR19973068.3147771/1‑150 (MQ=60)
                                                                                              AGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAT                                                       >  SRR19973068.1827650/1‑150 (MQ=60)
                                                                                               GCCAGAAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATG                                                      <  SRR19973068.3371694/150‑1 (MQ=60)
                                                                                                CCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGA                                                     >  SRR19973068.587124/1‑150 (MQ=60)
                                                                                                 CAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGAT                                                    >  SRR19973068.3216859/1‑150 (MQ=60)
                                                                                                    CAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCAC                                                 >  SRR19973068.654205/1‑150 (MQ=60)
                                                                                                     aatccgttcatttttcctgcCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACT                                                <  SRR19973068.1005467/130‑1 (MQ=60)
                                                                                                     AATACTTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACT                                                <  SRR19973068.1719676/150‑1 (MQ=60)
                                                                                                         gttaaatttgcccagcCAAACGCCCCGCGACCGGGTGGATACCTAAACGCAAATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAAT                                            <  SRR19973068.3884073/134‑1 (MQ=60)
                                                                                                          GTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATG                                           >  SRR19973068.1033853/1‑150 (MQ=60)
                                                                                                            TCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACTAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGG                                         >  SRR19973068.577415/1‑150 (MQ=60)
                                                                                                             CATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGA                                        <  SRR19973068.311534/150‑1 (MQ=60)
                                                                                                                  gccccgcaaccccCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGAAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTT                                   <  SRR19973068.1053151/137‑1 (MQ=60)
                                                                                                                  GTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTT                                   >  SRR19973068.2518333/1‑150 (MQ=60)
                                                                                                                   TCCAGGAAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTT                                  <  SRR19973068.3908080/150‑1 (MQ=60)
                                                                                                                     cagcaaacCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATCCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCA                                <  SRR19973068.1102347/142‑1 (MQ=60)
                                                                                                                       gccaacccCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGC                              <  SRR19973068.853039/142‑1 (MQ=60)
                                                                                                                       GGCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGC                              <  SRR19973068.853064/150‑1 (MQ=60)
                                                                                                                         CAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAG                            >  SRR19973068.3388536/1‑150 (MQ=60)
                                                                                                                          AAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGT                           >  SRR19973068.953652/1‑150 (MQ=60)
                                                                                                                          AAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAGTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGT                           >  SRR19973068.3023931/1‑150 (MQ=60)
                                                                                                                           AACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTAAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTT                          <  SRR19973068.2347925/150‑1 (MQ=60)
                                                                                                                             CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCC                        >  SRR19973068.1417135/1‑150 (MQ=60)
                                                                                                                              GCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCG                       <  SRR19973068.2140766/150‑1 (MQ=60)
                                                                                                                              GCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCG                       <  SRR19973068.681422/150‑1 (MQ=60)
                                                                                                                              GCCCCGCGACCGGGTGGATACCTAAACGCACATTAAGACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCG                       <  SRR19973068.2140813/150‑1 (MQ=60)
                                                                                                                               CCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGC                      >  SRR19973068.1384507/1‑150 (MQ=60)
                                                                                                                                 CCGCGACCGGGTGGATACCTAAACCCACATTAATACCACGAGCGCGCAATGTCACAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTG                    <  SRR19973068.2573883/150‑1 (MQ=60)
                                                                                                                                  CGCGACCGGGTGGATACCTAAACGCACATTAATACCCCGAGCGCGCAATTTCACCGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGT                   <  SRR19973068.1958066/150‑1 (MQ=60)
                                                                                                                                   GCGACCGGTTGGATAAATAAACGCAAATTAATACCAAGAGCGCGCAATTTCTCATTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGACGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTC                  <  SRR19973068.3345528/150‑1 (MQ=60)
                                                                                                                                     GACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTC                >  SRR19973068.2840581/1‑150 (MQ=60)
                                                                                                                                       CCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCAGCTGTCTCTT              <  SRR19973068.874316/150‑1 (MQ=60)
                                                                                                                                       CCGGGTGGATACCTAAACCCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCAGCTGTCTCTT              <  SRR19973068.873866/150‑1 (MQ=60)
                                                                                                                                                 ACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGAT    >  SRR19973068.1785178/1‑150 (MQ=60)
                                                                                                                                                   CTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTT  <  SRR19973068.3315429/150‑1 (MQ=60)
                                                                                                                                                   CTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTT  <  SRR19973068.941957/150‑1 (MQ=60)
                                                                                                                                                   CTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTT  <  SRR19973068.3545883/150‑1 (MQ=60)
                                                                                                                                                   CTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTACAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTT  <  SRR19973068.4022950/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
CGTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTT  >  CP009273/1669444‑1669740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑