Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 462,982 A→T 24.8% T39S (ACT→TCT)  cof → thiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273462,9820AT24.8% 459.5 / 172.5 226T39S (ACT→TCT) cofthiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase
Reads supporting (aligned to +/- strand):  ref base A (79/91);  new base T (34/22);  total (113/113)
Fisher's exact test for biased strand distribution p-value = 8.96e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

Only 100 of 229 total aligned reads displayed.

ATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCG  >  CP009273/462833‑463129
                                                                                                                                                     |                                                                                                                                                    
aTCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCa                                                                                                                                                      >  2:1892937/1‑150 (MQ=255)
   aCCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCtt                                                                                                                                                   >  1:1150017/1‑150 (MQ=255)
   aCCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACtt                                                                                                                                                   <  1:2289505/150‑1 (MQ=255)
     cATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACtttt                                                                                                                                                 <  1:861644/150‑1 (MQ=255)
        tAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGcc                                                                                                                                              <  1:383289/150‑1 (MQ=255)
         aaCCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCa                                                                                                                                             >  1:830838/1‑150 (MQ=255)
              tgtCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACgggg                                                                                                                                        >  2:3025383/1‑150 (MQ=255)
              tgtCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACgggg                                                                                                                                        >  2:1550274/1‑150 (MQ=255)
                    agagTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCAt                                                                                                                                  >  1:31680/1‑150 (MQ=255)
                    agagTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCAt                                                                                                                                  >  1:31628/1‑150 (MQ=255)
                     gagTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATg                                                                                                                                 <  1:957671/150‑1 (MQ=255)
                     gagTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATg                                                                                                                                 <  1:3505428/150‑1 (MQ=255)
                         ggAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCt                                                                                                                             >  1:1800621/1‑150 (MQ=255)
                             aaagaaaTGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGgag                                                                                                                         <  2:2191561/150‑1 (MQ=255)
                             aaagaaaTGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGgag                                                                                                                         <  2:1453017/150‑1 (MQ=255)
                                  aaTGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATgca                                                                                                                    >  2:2555547/1‑150 (MQ=255)
                                         cGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATAtt                                                                                                             >  1:1352429/1‑150 (MQ=255)
                                         cGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATAtt                                                                                                             >  2:1849535/1‑150 (MQ=255)
                                          gTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATtc                                                                                                            <  2:3647080/150‑1 (MQ=255)
                                             tGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTGTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCg                                                                                                         <  2:1229986/150‑1 (MQ=255)
                                             tGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCg                                                                                                         <  2:564271/150‑1 (MQ=255)
                                             tGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCg                                                                                                         >  1:1572951/1‑150 (MQ=255)
                                              ggCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCgg                                                                                                        >  1:305895/1‑150 (MQ=255)
                                              ggCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCgg                                                                                                        >  1:2350897/1‑150 (MQ=255)
                                              ggCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCgg                                                                                                        >  1:1265105/1‑150 (MQ=255)
                                               gcagcaTTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCggg                                                                                                       <  1:1258427/150‑1 (MQ=255)
                                                   caTTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGgcgc                                                                                                   >  2:2735722/1‑150 (MQ=255)
                                                    aTTTGATATGGATGGCACTTTATTGCTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCt                                                                                                  <  1:2854418/150‑1 (MQ=255)
                                                    aTTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCt                                                                                                  >  2:1362018/1‑150 (MQ=255)
                                                    aTTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCt                                                                                                  >  1:2308361/1‑150 (MQ=255)
                                                    aTTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCt                                                                                                  >  1:1880395/1‑150 (MQ=255)
                                                      ttGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTAt                                                                                                >  1:4048730/1‑150 (MQ=255)
                                                        gATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCg                                                                                              <  2:2201164/150‑1 (MQ=255)
                                                        gATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCg                                                                                              <  2:643853/150‑1 (MQ=255)
                                                          tatGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCt                                                                                            <  1:1263539/150‑1 (MQ=255)
                                                          tatGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCt                                                                                            >  2:1178944/1‑150 (MQ=255)
                                                           atggatggCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTg                                                                                           >  1:432235/1‑150 (MQ=255)
                                                              gatggCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGAt                                                                                        <  1:1034176/150‑1 (MQ=255)
                                                                 ggCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCg                                                                                     <  2:3908027/150‑1 (MQ=255)
                                                                 ggCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCg                                                                                     <  1:2690351/150‑1 (MQ=255)
                                                                  gCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGt                                                                                    >  2:1957215/1‑150 (MQ=255)
                                                                      tttATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCCCGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑ttt                                                                               <  2:299442/150‑1 (MQ=255)
                                                                         aTTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGAt                                                                            <  1:2516936/150‑1 (MQ=255)
                                                                           tGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTa                                                                          <  2:3609540/150‑1 (MQ=255)
                                                                           tGATGCCCGACAATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCAATCGCTGGATGCGTA‑TTTGATTa                                                                          >  2:1561145/1‑150 (MQ=255)
                                                                                 ccGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCa                                                                    <  2:1645086/150‑1 (MQ=255)
                                                                                   gACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAAc                                                                  >  2:3474712/1‑150 (MQ=255)
                                                                                   gACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAAc                                                                  >  1:617425/1‑150 (MQ=255)
                                                                                   gACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAAc                                                                  <  2:3594014/150‑1 (MQ=255)
                                                                                   gACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAAc                                                                  >  2:2922357/1‑150 (MQ=255)
                                                                                    aCCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACg                                                                 >  2:844475/1‑150 (MQ=255)
                                                                                       atcatTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGaa                                                              <  1:2075404/150‑1 (MQ=255)
                                                                                         catTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAAcg                                                            >  1:2778592/1‑150 (MQ=255)
                                                                                          atTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAAcgc                                                           >  2:3687227/1‑150 (MQ=255)
                                                                                              aGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGt                                                       >  2:1694353/1‑150 (MQ=255)
                                                                                                gTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGc                                                     <  1:3982991/150‑1 (MQ=255)
                                                                                                 tGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCa                                                    >  1:2504806/1‑150 (MQ=255)
                                                                                                      aaaCCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATtctc                                               <  2:2397613/150‑1 (MQ=255)
                                                                                                      aaaCCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATAGTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATtctc                                               <  2:2397704/150‑1 (MQ=255)
                                                                                                       aaCCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTctct                                              >  2:3781065/1‑150 (MQ=255)
                                                                                                       aaCCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTctct                                              <  2:1410115/150‑1 (MQ=255)
                                                                                                        aCCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTTGATTACCGGCAACGGAACGCGCGTGCATTctct                                              <  1:542260/150‑1 (MQ=255)
                                                                                                        aCCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTg                                             <  1:2976406/150‑1 (MQ=255)
                                                                                                         cccTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTgg                                            >  1:728009/1‑150 (MQ=255)
                                                                                                            tctctACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAg                                         >  2:2650339/1‑150 (MQ=255)
                                                                                                             ctctACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAgg                                        <  1:3416719/150‑1 (MQ=255)
                                                                                                              tctACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGt                                       <  1:2437403/150‑1 (MQ=255)
                                                                                                                tACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGa                                     >  2:2800471/1‑150 (MQ=255)
                                                                                                                 aCTTTGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGaa                                    >  2:1531738/1‑150 (MQ=255)
                                                                                                                 aCTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGaa                                    <  2:3902354/150‑1 (MQ=255)
                                                                                                                 aCTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGaa                                    <  2:4130168/150‑1 (MQ=255)
                                                                                                                   tttGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACt                                  <  2:3895676/150‑1 (MQ=255)
                                                                                                                     tGGCGCGACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACttt                                >  1:3296398/1‑150 (MQ=255)
                                                                                                                        cgcgACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTAc                             <  2:232422/150‑1 (MQ=255)
                                                                                                                        cgcgACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTAc                             >  1:3277457/1‑150 (MQ=255)
                                                                                                                          cgACTGCGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACAt                           >  2:3163998/1‑150 (MQ=255)
                                                                                                                            aCTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCg                         <  2:1079806/150‑1 (MQ=255)
                                                                                                                              tGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGtg                       >  2:2379218/1‑150 (MQ=255)
                                                                                                                                cGTGAACGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGtgat                     <  2:3730087/150‑1 (MQ=255)
                                                                                                                                cGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGtgat                     >  1:2787950/1‑150 (MQ=255)
                                                                                                                                  tGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGtgatga                   <  2:2308235/150‑1 (MQ=255)
                                                                                                                                  tGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGtgatga                   <  2:1506021/150‑1 (MQ=255)
                                                                                                                                     aCGCGACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGAttt                <  2:1924689/150‑1 (MQ=255)
                                                                                                                                     aCGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGAttt                <  2:2074685/150‑1 (MQ=255)
                                                                                                                                       gcgACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTAc              >  2:1224369/1‑150 (MQ=255)
                                                                                                                                        cgACATTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTAcc             >  1:1470693/1‑150 (MQ=255)
                                                                                                                                            aTTACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCg         >  2:1770492/1‑150 (MQ=255)
                                                                                                                                            aTTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCg         >  1:1891470/1‑150 (MQ=255)
                                                                                                                                            aTTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCg         >  2:891300/1‑150 (MQ=255)
                                                                                                                                             ttACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCgg        >  2:1794346/1‑150 (MQ=255)
                                                                                                                                             ttACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCgg        >  2:3339092/1‑150 (MQ=255)
                                                                                                                                             ttACCCTCTCTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCgg        >  2:1794397/1‑150 (MQ=255)
                                                                                                                                             ttACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCgg        >  1:2648823/1‑150 (MQ=255)
                                                                                                                                             ttACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCgg        >  1:2648816/1‑150 (MQ=255)
                                                                                                                                               aCCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGAt      >  1:3731023/1‑150 (MQ=255)
                                                                                                                                               aCCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGAt      <  1:710650/150‑1 (MQ=255)
                                                                                                                                                 ccTCTCTTGTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGt    <  2:578105/150‑1 (MQ=255)
                                                                                                                                                 ccTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGt    <  2:3570306/150‑1 (MQ=255)
                                                                                                                                                  cTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTc   <  2:3151180/150‑1 (MQ=255)
                                                                                                                                                  cTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTc   <  1:4164058/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTA‑TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCG  >  CP009273/462833‑463129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A