Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 465,341 A→C 41.8% I339L (ATT→CTT)  mdlA → putative multidrug transporter subunit of ABC superfamily, ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273465,3410AC41.8% 46.0 / inf 220I339L (ATT→CTT) mdlAputative multidrug transporter subunit of ABC superfamily, ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base A (68/59);  new base C (33/59);  total (101/119)
Fisher's exact test for biased strand distribution p-value = 1.33e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 221 total aligned reads displayed.

CCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCATTTCGACGTC  >  CP009273/465194‑465490
                                                                                                                                                   |                                                                                                                                                     
ccAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATAtt                                                                                                                                                     >  2:2285670/1‑150 (MQ=255)
  aaTGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCa                                                                                                                                                   <  2:1295366/150‑1 (MQ=255)
    tGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCAcc                                                                                                                                                 >  1:1929478/1‑150 (MQ=255)
     gctggcgctggcATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCa                                                                                                                                                <  1:45065/150‑1 (MQ=255)
     gctggcgctggcATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCa                                                                                                                                                <  2:3275520/150‑1 (MQ=255)
      ctggcgctggcATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAg                                                                                                                                               >  1:419670/1‑150 (MQ=255)
       tggcgctggcATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGt                                                                                                                                              >  1:1526571/1‑150 (MQ=255)
        ggcgctggcATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGtt                                                                                                                                             >  1:3881012/1‑150 (MQ=255)
        ggcgctggcATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGtt                                                                                                                                             >  1:3779406/1‑150 (MQ=255)
        ggcgctggcATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGtt                                                                                                                                             >  2:3484727/1‑150 (MQ=255)
         gcgctggcATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTc                                                                                                                                            <  2:2231767/150‑1 (MQ=255)
          cgctggcATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCa                                                                                                                                           <  2:3612867/150‑1 (MQ=255)
             tggcATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGt                                                                                                                                        >  2:947920/1‑150 (MQ=255)
              ggcATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTa                                                                                                                                       >  1:3638241/1‑150 (MQ=255)
              ggcATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTa                                                                                                                                       >  1:1665707/1‑150 (MQ=255)
               gcATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTAt                                                                                                                                      <  2:3881394/150‑1 (MQ=255)
               gcATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTAt                                                                                                                                      <  2:1214599/150‑1 (MQ=255)
               gcATGGATGTTTAACAGTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTAt                                                                                                                                      <  1:958775/150‑1 (MQ=255)
                 aTGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGAGGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATcc                                                                                                                                    <  2:3973120/150‑1 (MQ=255)
                  tGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCg                                                                                                                                   >  2:2128265/1‑150 (MQ=255)
                  tGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCg                                                                                                                                   >  1:2099429/1‑150 (MQ=255)
                   ggATGATTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGc                                                                                                                                  <  2:2337853/150‑1 (MQ=255)
                       gTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGAc                                                                                                                              >  1:1463389/1‑150 (MQ=255)
                       gTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGAc                                                                                                                              >  1:1463361/1‑150 (MQ=255)
                        tttAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCTGTTCACGTATCCGCAGACt                                                                                                                             <  2:458523/150‑1 (MQ=255)
                         ttAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTg                                                                                                                            <  2:130369/150‑1 (MQ=255)
                         ttAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTg                                                                                                                            <  1:2876229/150‑1 (MQ=255)
                         ttAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTg                                                                                                                            <  2:2086317/150‑1 (MQ=255)
                           aaCATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGAc                                                                                                                          >  1:1310004/1‑150 (MQ=255)
                                  tgGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTg                                                                                                                   <  2:3344143/150‑1 (MQ=255)
                                  tgGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGAGGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTg                                                                                                                   <  1:691636/150‑1 (MQ=255)
                                   ggAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTgc                                                                                                                  >  2:1659978/1‑150 (MQ=255)
                                     aaCGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTgcgc                                                                                                                >  2:2541808/1‑150 (MQ=255)
                                     aaCGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTgcgc                                                                                                                >  2:122914/1‑150 (MQ=255)
                                      aCGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCt                                                                                                               <  2:3018854/150‑1 (MQ=255)
                                       cGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTg                                                                                                              >  2:1304735/1‑150 (MQ=255)
                                        gTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTgg                                                                                                             >  1:2578773/1‑150 (MQ=255)
                                           gTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGaaa                                                                                                          >  1:194223/1‑150 (MQ=255)
                                                gctgcGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTc                                                                                                     >  1:3098320/1‑150 (MQ=255)
                                                gctgcGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTc                                                                                                     <  1:3365062/150‑1 (MQ=255)
                                                  tgcGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCaa                                                                                                   <  1:3317736/150‑1 (MQ=255)
                                                   gcGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAAt                                                                                                  <  2:2854826/150‑1 (MQ=255)
                                                          gCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGccc                                                                                           <  2:647632/150‑1 (MQ=255)
                                                             gTATTCGCGCGATGCTGGCGGAAGCGCAGGTGGTGAACGATGGTAGAGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACTTATCCGCAGACTGACCATCCTGATCTGGAAAACGTCAATTTCGCCCTGa                                                                                        <  1:769019/150‑1 (MQ=255)
                                                              tATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGaa                                                                                       <  2:2233129/150‑1 (MQ=255)
                                                              tATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGaa                                                                                       >  2:3147446/1‑150 (MQ=255)
                                                                ttCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAAc                                                                                     <  1:3434872/150‑1 (MQ=255)
                                                                       gATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCa                                                                              <  1:1751572/150‑1 (MQ=255)
                                                                        aTGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAg                                                                             >  1:2801214/1‑150 (MQ=255)
                                                                         tGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGa                                                                            >  1:2912081/1‑150 (MQ=255)
                                                                           cTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCTCATGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAAACCGTTCAGATg                                                                          <  2:95279/150‑1 (MQ=255)
                                                                           cTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATg                                                                          <  2:1987800/150‑1 (MQ=255)
                                                                            tGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGc                                                                         <  2:480643/150‑1 (MQ=255)
                                                                               cGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTgg                                                                      >  1:1587464/1‑150 (MQ=255)
                                                                               cGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTgg                                                                      <  2:1148236/150‑1 (MQ=255)
                                                                                ggAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTggg                                                                     <  2:1266757/150‑1 (MQ=255)
                                                                                ggAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTggg                                                                     <  2:3439932/150‑1 (MQ=255)
                                                                                ggAAGAGCCGGTGGTGAACGATGGTATCGAACCCGTGCCGGAAGGGCGTGGAGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCATACTTACCATCCTGCGCTGGAAAACGTCAATTTCGAACTGAAACCCGGTCAGATGCTggg                                                                     <  2:369171/150‑1 (MQ=255)
                                                                                   aGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTAt                                                                  >  1:2213222/1‑150 (MQ=255)
                                                                                    gcgcCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATTTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATc                                                                 <  1:538692/150‑1 (MQ=255)
                                                                                    gcgcCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATc                                                                 <  1:606558/150‑1 (MQ=255)
                                                                                       ccGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGc                                                              <  2:3847712/150‑1 (MQ=255)
                                                                                        cGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCg                                                             >  2:2502237/1‑150 (MQ=255)
                                                                                         ggtggtGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCgg                                                            <  2:743827/150‑1 (MQ=255)
                                                                                           tggtgAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGc                                                          >  2:2035261/1‑150 (MQ=255)
                                                                                            ggtgAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGcc                                                         <  1:397569/150‑1 (MQ=255)
                                                                                             gtgAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCg                                                        <  1:1029461/150‑1 (MQ=255)
                                                                                              tgAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGa                                                       >  2:279138/1‑150 (MQ=255)
                                                                                              tgAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGa                                                       <  2:3520811/150‑1 (MQ=255)
                                                                                                  cGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTgg                                                   <  2:3843467/150‑1 (MQ=255)
                                                                                                   gATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGt                                                  >  2:552228/1‑150 (MQ=255)
                                                                                                    aTGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGtt                                                 <  1:751812/150‑1 (MQ=255)
                                                                                                     tGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTc                                                >  2:3805006/1‑150 (MQ=255)
                                                                                                      ggTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTcc                                               <  2:2754396/150‑1 (MQ=255)
                                                                                                      ggTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTcc                                               <  2:3103542/150‑1 (MQ=255)
                                                                                                       gTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCg                                              >  1:2775923/1‑150 (MQ=255)
                                                                                                          gCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCa                                           <  1:350811/150‑1 (MQ=255)
                                                                                                                   tGCCGGAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTAccc                                  >  2:802558/1‑150 (MQ=255)
                                                                                                                       ggAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCtgtt                              >  1:2265397/1‑150 (MQ=255)
                                                                                                                       ggAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCtgtt                              <  1:2238038/150‑1 (MQ=255)
                                                                                                                       ggAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCtgtt                              <  2:1529372/150‑1 (MQ=255)
                                                                                                                        gAAGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCtgttg                             >  1:3079189/1‑150 (MQ=255)
                                                                                                                         aaGGGCGTGGCGAACTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCtgttgt                            >  2:1784021/1‑150 (MQ=255)
                                                                                                                         aaGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCtgttgt                            >  2:2402003/1‑150 (MQ=255)
                                                                                                                          aGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTc                           >  1:3266364/1‑150 (MQ=255)
                                                                                                                                    gAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAg                 >  2:176662/1‑150 (MQ=255)
                                                                                                                                     aaCTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGc                >  1:2309764/1‑150 (MQ=255)
                                                                                                                                     aaCTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGc                >  2:2672785/1‑150 (MQ=255)
                                                                                                                                     aaCTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGc                <  2:1061321/150‑1 (MQ=255)
                                                                                                                                       cTGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTAGTCGCTCATTCAGCGt              >  1:2741434/1‑150 (MQ=255)
                                                                                                                                        tGGATGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTc             >  1:1189002/1‑150 (MQ=255)
                                                                                                                                          gATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCAt           <  1:2775430/150‑1 (MQ=255)
                                                                                                                                          gATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGATGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCAt           <  1:1730220/150‑1 (MQ=255)
                                                                                                                                           aTGTAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCAtt          <  1:1824814/150‑1 (MQ=255)
                                                                                                                                           aTGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCAtt          <  2:1166274/150‑1 (MQ=255)
                                                                                                                                           aTGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCAtt          >  1:711827/1‑150 (MQ=255)
                                                                                                                                              tAAATCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCATTTCg       <  2:4125267/150‑1 (MQ=255)
                                                                                                                                                aaTCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCATTTCGAc     <  1:3032524/150‑1 (MQ=255)
                                                                                                                                                aaTCTTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCATTTCGAc     <  1:204753/150‑1 (MQ=255)
                                                                                                                                                   atTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCATTTCGACGTc  >  1:2373618/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAGTTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCATTTCGACGTC  >  CP009273/465194‑465490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A