Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,663,893 C→T 27.5% intergenic (‑44/‑63) ynfL ← / → ynfM LysR family putative transcriptional regulator/putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,663,8930CT27.5% 320.7 / 150.7 190intergenic (‑44/‑63)ynfL/ynfMLysR family putative transcriptional regulator/putative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base C (56/79);  new base T (27/25);  total (84/106)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01

Only 100 of 197 total aligned reads displayed.

GACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAATCAATTTATTAAACGC  >  CP009273/1663744‑1664042
                                                                                                                                                     |                                                                                                                                                     
gACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAAc                                                                                                                                                       <  2:4146648/150‑1 (MQ=255)
gACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAAc                                                                                                                                                       >  2:3635515/1‑150 (MQ=255)
 aCTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACa                                                                                                                                                      <  1:975371/150‑1 (MQ=255)
   ttAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGaataat                                                                                                                                                    >  2:2030886/1‑150 (MQ=255)
    tAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAAtt                                                                                                                                                   >  1:3542739/1‑150 (MQ=255)
    tAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAAtt                                                                                                                                                   >  1:3542307/1‑150 (MQ=255)
        ggcggTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAGTATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAATAATTCGAt                                                                                                                                               <  1:2918592/150‑1 (MQ=255)
        ggcggTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAATAATTCGAt                                                                                                                                               <  1:1896998/150‑1 (MQ=255)
        ggcggTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGAt                                                                                                                                               >  1:3028240/1‑150 (MQ=255)
         gcggTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAATAATTCGAtt                                                                                                                                              <  1:4055335/150‑1 (MQ=255)
           ggTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATCTTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGACTAATTCGAtttt                                                                                                                                            >  2:3412551/1‑150 (MQ=255)
             ttGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTcc                                                                                                                                          >  1:418661/1‑150 (MQ=255)
              tGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCt                                                                                                                                         <  2:1968930/150‑1 (MQ=255)
                  aaaTATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGAAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGAGTATTAATATATTAGACAGAACAATTCGATTTTCCTAccc                                                                                                                                     >  2:1205320/1‑150 (MQ=255)
                   aaTATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCt                                                                                                                                    <  2:1302348/150‑1 (MQ=255)
                     tatTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTAt                                                                                                                                  <  2:1173220/150‑1 (MQ=255)
                      atTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATg                                                                                                                                 <  1:4274338/150‑1 (MQ=255)
                      atTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATg                                                                                                                                 <  1:3285465/150‑1 (MQ=255)
                         cAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGtat                                                                                                                              <  2:1736087/150‑1 (MQ=255)
                           ggCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATaa                                                                                                                            <  2:3163856/150‑1 (MQ=255)
                             cGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGc                                                                                                                          <  1:4501828/150‑1 (MQ=255)
                                  aGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGAt                                                                                                                     <  2:2229912/150‑1 (MQ=255)
                                   gcggcgCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATc                                                                                                                    <  1:349698/150‑1 (MQ=255)
                                   gcggcgCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATc                                                                                                                    <  1:349705/150‑1 (MQ=255)
                                    cggcgCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCt                                                                                                                   <  2:2231211/150‑1 (MQ=255)
                                     ggcgCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTCTAAGCCTGATCTa                                                                                                                  >  2:882778/1‑150 (MQ=255)
                                     ggcgCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTa                                                                                                                  >  2:203311/1‑150 (MQ=255)
                                        gagcCCGAAATGCAGCTCTACCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAg                                                                                                               <  2:3699375/148‑1 (MQ=255)
                                             cGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCata                                                                                                          <  1:4009660/150‑1 (MQ=255)
                                              gAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCatat                                                                                                         <  1:3627993/150‑1 (MQ=255)
                                              gAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCatat                                                                                                         <  1:240534/150‑1 (MQ=255)
                                              gAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCatat                                                                                                         <  1:3627987/150‑1 (MQ=255)
                                                  tGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAg                                                                                                     <  1:1631513/150‑1 (MQ=255)
                                                    cAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCa                                                                                                   <  2:2969467/150‑1 (MQ=255)
                                                                 acagcaacaAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTg                                                                                      <  2:2495270/150‑1 (MQ=255)
                                                                   agcaacaAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAg                                                                                    >  2:906698/1‑150 (MQ=255)
                                                                        caaAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGta                                                                               >  1:3582981/1‑150 (MQ=255)
                                                                        caaAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGta                                                                               <  1:2634342/150‑1 (MQ=255)
                                                                            gTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGtactac                                                                           >  1:4066170/1‑150 (MQ=255)
                                                                                cGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACt                                                                       <  1:2111398/150‑1 (MQ=255)
                                                                                  cAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGt                                                                     <  1:4617555/150‑1 (MQ=255)
                                                                                   aGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGtt                                                                    >  2:2658580/1‑150 (MQ=255)
                                                                                    gATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTg                                                                   <  2:2697986/150‑1 (MQ=255)
                                                                                         cGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGgc                                                              <  2:1973717/150‑1 (MQ=255)
                                                                                          gAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCATACCCTATGAATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGAGAGCCGTACTACAACTGTTGATGgcg                                                             >  1:4086384/1‑150 (MQ=255)
                                                                                              ttCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTCTGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTcc                                                         <  1:2685047/150‑1 (MQ=255)
                                                                                               tCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCg                                                        <  1:3726869/150‑1 (MQ=255)
                                                                                                cAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCgg                                                       <  1:506716/150‑1 (MQ=255)
                                                                                                cAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCgg                                                       <  1:122954/150‑1 (MQ=255)
                                                                                                 aaTATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGc                                                      <  2:333116/150‑1 (MQ=255)
                                                                                                  atatTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCa                                                     >  2:1198291/1‑150 (MQ=255)
                                                                                                  atatTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCa                                                     >  2:444168/1‑150 (MQ=255)
                                                                                                     ttCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGc                                                  <  2:3048977/150‑1 (MQ=255)
                                                                                                     ttCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGc                                                  <  2:2700991/150‑1 (MQ=255)
                                                                                                     ttCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGc                                                  <  2:3047996/150‑1 (MQ=255)
                                                                                                      tCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCg                                                 >  1:4527551/1‑150 (MQ=255)
                                                                                                         tatTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGaca                                              >  1:1372190/1‑150 (MQ=255)
                                                                                                         tatTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGaca                                              <  2:168901/150‑1 (MQ=255)
                                                                                                           tttAAAACATCTTATTTGAGATTATTAATTTATTAGACATAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGAAAGTTTTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACt                                            <  2:2326817/150‑1 (MQ=255)
                                                                                                              aaaaCATCTTATTTGAGATTATTAATATATTAGACAGAAAAATTCGATTTTACTACCATATGTATAAGACTGATCTAAAGGCATATTTAGCAAGGATTTCAAGTTAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGAc                                         >  1:3563903/1‑150 (MQ=255)
                                                                                                                 aCATCTTATTTGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACaag                                      >  2:1433954/1‑150 (MQ=255)
                                                                                                                 aCATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTACCAACCCTATGTATAAGCCTGATCTACAGGCATATTAAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTAGATGGCGCTCCGGCAAGCGACACTGACaag                                      >  2:1693864/1‑150 (MQ=255)
                                                                                                                  cATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACaagc                                     <  1:3224399/150‑1 (MQ=255)
                                                                                                                   aTCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACaagca                                    >  2:4077928/1‑150 (MQ=255)
                                                                                                                      ttATTTGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACaagcaaag                                 >  1:2249760/1‑150 (MQ=255)
                                                                                                                      ttATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACaagcaaag                                 >  2:1375576/1‑150 (MQ=255)
                                                                                                                         tttGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCAt                              >  1:4689359/1‑150 (MQ=255)
                                                                                                                          ttGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATTTATAAGCCTGATCTACAGGAATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCAtt                             <  1:100907/150‑1 (MQ=255)
                                                                                                                          ttGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCAtt                             >  2:2344598/1‑150 (MQ=255)
                                                                                                                           tGATATTATTAATATATTAGACATAACAATTCTATTTTCCTACCCTATGTATAAGCCTTATCTACAGGAATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGTGCTCCGGCAAGCGACACTGACAAGCAAAGCAttt                            <  1:4414547/150‑1 (MQ=255)
                                                                                                                           tGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCAttt                            <  2:35211/150‑1 (MQ=255)
                                                                                                                           tGAGATTATTAATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCAttt                            >  2:4587345/1‑150 (MQ=255)
                                                                                                                           tGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCAttt                            >  1:2722573/1‑150 (MQ=255)
                                                                                                                           tGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCAttt                            <  2:4554939/150‑1 (MQ=255)
                                                                                                                           tGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCAttt                            <  2:2104336/150‑1 (MQ=255)
                                                                                                                             agaTTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTtct                          >  1:3883212/1‑150 (MQ=255)
                                                                                                                              gaTTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCTTATTTAGCATGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCACGCGACACTGACAAGCAAAGCATTtctc                         <  1:2156266/150‑1 (MQ=255)
                                                                                                                                ttattaATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAg                       >  1:1534021/1‑150 (MQ=255)
                                                                                                                                ttattaATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAg                       >  2:2681931/1‑150 (MQ=255)
                                                                                                                                ttattaATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAg                       <  1:3918451/150‑1 (MQ=255)
                                                                                                                                 tattaATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCACGCGACACTGACAAGCTACGCAGTTCTCAGc                      >  2:2735714/1‑150 (MQ=255)
                                                                                                                                  attaATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGcc                     >  1:3599454/1‑150 (MQ=255)
                                                                                                                                   ttaATATATTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCa                    >  1:1146590/1‑150 (MQ=255)
                                                                                                                                   ttaATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCa                    >  1:244309/1‑150 (MQ=255)
                                                                                                                                      atatatTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAAt                 >  1:2289872/1‑150 (MQ=255)
                                                                                                                                      atatatTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAAt                 >  2:4271396/1‑150 (MQ=255)
                                                                                                                                       tatatTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAATc                <  2:713551/150‑1 (MQ=255)
                                                                                                                                       tatatTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAATc                <  2:4712030/150‑1 (MQ=255)
                                                                                                                                          atTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAATCAAt             >  1:400912/1‑150 (MQ=255)
                                                                                                                                          atTAGACAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAATCAAt             >  1:28905/1‑150 (MQ=255)
                                                                                                                                           ttAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAATCAAtt            <  2:2398149/150‑1 (MQ=255)
                                                                                                                                             aGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAATCAATtta          <  2:608361/150‑1 (MQ=255)
                                                                                                                                             aGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGAATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAATCAATtta          <  1:3130697/150‑1 (MQ=255)
                                                                                                                                               aCAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAATCAATttatt        >  1:1945100/1‑150 (MQ=255)
                                                                                                                                                cAGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAATCAATttatta       >  1:1388888/1‑150 (MQ=255)
                                                                                                                                                cAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAATCAATttatta       >  2:3824718/1‑150 (MQ=255)
                                                                                                                                                 aGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAATCAATTTATTaa      >  1:3090930/1‑150 (MQ=255)
                                                                                                                                                 aGAATAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAATCAATTTATTaa      >  2:527442/1‑150 (MQ=255)
                                                                                                                                                   aaCAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAATCAATTTATTAAAc    >  2:4250809/1‑150 (MQ=255)
                                                                                                                                                     cAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAATCAATTTATTAAAcgc  >  1:2490080/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTACCCTATGTATAAGCCTGATCTACAGGCATATTTAGCAAGGATTTCAAGTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAATCAATTTATTAAACGC  >  CP009273/1663744‑1664042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A