Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,865,422 C→A 72.3% R261R (CGA→AGA)  yeaI → putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,865,4220CA72.3% 313.0 / 195.7 206R261R (CGA→AGA) yeaIputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (32/25);  new base A (65/83);  total (97/109)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 206 total aligned reads displayed.

CACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGT  >  CP009273/1865275‑1865567
                                                                                                                                                   |                                                                                                                                                 
cacaATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGa                                                                                                                                                 <  1:2106683/150‑1 (MQ=255)
 acaATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGAt                                                                                                                                                <  2:1948708/150‑1 (MQ=255)
 acaATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGAt                                                                                                                                                <  2:1102142/150‑1 (MQ=255)
   aaTCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTa                                                                                                                                              >  2:909293/1‑150 (MQ=255)
   aaTCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTa                                                                                                                                              >  2:4342890/1‑150 (MQ=255)
            aGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGaa                                                                                                                                     >  1:236930/1‑150 (MQ=255)
            aGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGaa                                                                                                                                     >  1:4363947/1‑150 (MQ=255)
            aGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGaa                                                                                                                                     >  1:3117077/1‑150 (MQ=255)
             gTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAAc                                                                                                                                    >  2:1467059/1‑150 (MQ=255)
             gTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAAc                                                                                                                                    >  2:3925611/1‑150 (MQ=255)
             gTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAAc                                                                                                                                    <  2:3430331/150‑1 (MQ=255)
               tataACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTa                                                                                                                                  >  1:2541793/1‑150 (MQ=255)
                  aaCGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATgg                                                                                                                               >  1:1974264/1‑150 (MQ=255)
                  aaCGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATgg                                                                                                                               >  2:2426061/1‑150 (MQ=255)
                  aaCGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGGTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATgg                                                                                                                               >  1:2504054/1‑150 (MQ=255)
                    cGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGcc                                                                                                                             >  2:2405390/1‑150 (MQ=255)
                    cGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGcc                                                                                                                             >  2:2405412/1‑150 (MQ=255)
                        gACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTa                                                                                                                         <  2:3594000/150‑1 (MQ=255)
                        gACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTa                                                                                                                         <  2:640690/150‑1 (MQ=255)
                        gACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTa                                                                                                                         <  2:1792681/150‑1 (MQ=255)
                         aCTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTaa                                                                                                                        <  1:3151402/150‑1 (MQ=255)
                           tATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATa                                                                                                                      >  2:3999663/1‑150 (MQ=255)
                             ttCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGc                                                                                                                    >  2:858970/1‑150 (MQ=255)
                                  tGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCattat                                                                                                               <  2:4227799/150‑1 (MQ=255)
                                   gTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCattatt                                                                                                              <  1:898456/150‑1 (MQ=255)
                                   gTATGTCGTCGATTACTGTCCGGACAACCAGACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCattatt                                                                                                              <  1:899404/150‑1 (MQ=255)
                                    tATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCAttatta                                                                                                             <  2:602595/150‑1 (MQ=255)
                                    tATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCAttatta                                                                                                             >  1:1314277/1‑150 (MQ=255)
                                     aTGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTATCCAGCGAACTATTGCCGTTAATAGAATtattat                                                                                                            >  2:2703104/1‑150 (MQ=255)
                                      tGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATg                                                                                                           <  1:893180/150‑1 (MQ=255)
                                         cgtcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCt                                                                                                        >  2:2467216/1‑150 (MQ=255)
                                           tcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTgg                                                                                                      >  1:708849/1‑150 (MQ=255)
                                             gATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCa                                                                                                    <  1:4746577/150‑1 (MQ=255)
                                             gATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCa                                                                                                    <  1:1557399/150‑1 (MQ=255)
                                              aTTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCAt                                                                                                   <  2:1426447/150‑1 (MQ=255)
                                              aTTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCAt                                                                                                   <  1:3028936/150‑1 (MQ=255)
                                                    gTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAt                                                                                             <  1:2405390/150‑1 (MQ=255)
                                                    gTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAt                                                                                             <  2:1392853/150‑1 (MQ=255)
                                                    gTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAt                                                                                             <  1:2405412/150‑1 (MQ=255)
                                                     tCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAtg                                                                                            <  2:2212192/150‑1 (MQ=255)
                                                       cGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAtgct                                                                                          >  1:553856/1‑150 (MQ=255)
                                                           cAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCaa                                                                                      >  1:2140067/1‑150 (MQ=255)
                                                           cAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCaa                                                                                      >  1:3645116/1‑150 (MQ=255)
                                                           cAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCaa                                                                                      >  1:4243740/1‑150 (MQ=255)
                                                                aTACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAacttac                                                                                 >  2:3028845/1‑150 (MQ=255)
                                                                   cTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTc                                                                              <  2:2748689/150‑1 (MQ=255)
                                                                   cTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTc                                                                              >  1:618439/1‑150 (MQ=255)
                                                                     gCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTa                                                                            <  1:4435623/150‑1 (MQ=255)
                                                                      cGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTAc                                                                           >  2:1687811/1‑150 (MQ=255)
                                                                        aCCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTg                                                                         >  2:2234041/1‑150 (MQ=255)
                                                                         ccTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGa                                                                        <  2:4626244/150‑1 (MQ=255)
                                                                          cTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGAc                                                                       >  2:1520941/1‑150 (MQ=255)
                                                                           tGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGAct                                                                      >  1:3185061/1‑150 (MQ=255)
                                                                           tGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGAct                                                                      >  2:4639326/1‑150 (MQ=255)
                                                                             gggAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGActct                                                                    <  1:1713102/150‑1 (MQ=255)
                                                                             gggAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGActct                                                                    <  1:4676587/150‑1 (MQ=255)
                                                                               gAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTgg                                                                  <  2:4232238/150‑1 (MQ=255)
                                                                                aaTCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGa                                                                 >  2:1457785/1‑150 (MQ=255)
                                                                                 aTCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGAt                                                                <  2:4758800/150‑1 (MQ=255)
                                                                                 aTCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGAt                                                                <  1:4277253/150‑1 (MQ=255)
                                                                                  tCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATg                                                               <  2:3337354/150‑1 (MQ=255)
                                                                                    aaCTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAg                                                             <  1:3786807/150‑1 (MQ=255)
                                                                                       tataCAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGtat                                                          >  2:1055393/1‑150 (MQ=255)
                                                                                         taCAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATaa                                                        <  2:1195229/150‑1 (MQ=255)
                                                                                         taCAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATaa                                                        <  1:3161380/150‑1 (MQ=255)
                                                                                           cAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAAtt                                                      >  2:1926295/1‑150 (MQ=255)
                                                                                           cAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAAtt                                                      >  2:1879031/1‑150 (MQ=255)
                                                                                            aaaaaTATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATta                                                     <  1:641999/150‑1 (MQ=255)
                                                                                             aaaaTATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATtat                                                    <  2:236930/150‑1 (MQ=255)
                                                                                              aaaTATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATtata                                                   >  1:1762748/1‑150 (MQ=255)
                                                                                                atatTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACt                                                 >  1:4736754/1‑150 (MQ=255)
                                                                                                 tatTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTa                                                >  2:211887/1‑150 (MQ=255)
                                                                                                   ttGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATc                                              <  2:1203429/150‑1 (MQ=255)
                                                                                                   ttGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATc                                              >  1:4172808/1‑150 (MQ=255)
                                                                                                     ggTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTg                                            >  2:3728788/1‑150 (MQ=255)
                                                                                                     ggTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTg                                            <  1:3872407/150‑1 (MQ=255)
                                                                                                       tttGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGt                                          <  1:2978491/150‑1 (MQ=255)
                                                                                                          gTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGtata                                       >  2:4535932/1‑150 (MQ=255)
                                                                                                           tCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTatat                                      >  2:3755695/1‑150 (MQ=255)
                                                                                                                gggCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATTTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTc                                 <  2:4525824/150‑1 (MQ=255)
                                                                                                                gggCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTc                                 >  1:3975013/1‑150 (MQ=255)
                                                                                                                   cATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTcgcg                              <  1:1416307/150‑1 (MQ=255)
                                                                                                                    aTTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCgg                             >  1:277800/1‑150 (MQ=255)
                                                                                                                      ttttACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGa                           >  2:2641878/1‑150 (MQ=255)
                                                                                                                       tttACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGAGAACTATGGCAGTTAATAGCATTATTATTTCTGGCATCGCTATGCTGCAAATTACTTCTACTGACTCTGGATGATTATAATTATACTATCTGGTATATAAGTCGCGGGAt                          <  1:983186/150‑1 (MQ=255)
                                                                                                                               tCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGttt                  <  1:2088239/150‑1 (MQ=255)
                                                                                                                                 tttATTATCATGCGCACAATATTAGCCAGCGAACTATGTACGTTAATAGAATTATTATGTCTTGCATATCTATGCTGCAAATTAATTCTACTTACTCTGGATGAGTATAATTATACTATATTGTATATAAGTAGCGGGATTTAAttttac                >  1:378609/1‑148 (MQ=255)
                                                                                                                                  ttattaTCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCa               <  1:1096669/150‑1 (MQ=255)
                                                                                                                                   tattatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAg              <  1:3456661/150‑1 (MQ=255)
                                                                                                                                      tatCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTaa           <  2:4223183/150‑1 (MQ=255)
                                                                                                                                      tatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTaa           <  2:4456799/150‑1 (MQ=255)
                                                                                                                                      tatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTaa           >  1:2316168/1‑150 (MQ=255)
                                                                                                                                      tatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTaa           <  2:1304102/150‑1 (MQ=255)
                                                                                                                                      tatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTACAGTaa           >  1:2884060/1‑150 (MQ=255)
                                                                                                                                         cATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACt        >  2:4127466/1‑150 (MQ=255)
                                                                                                                                         cATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACt        <  1:780196/150‑1 (MQ=255)
                                                                                                                                           tGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgt      >  2:2936380/1‑150 (MQ=255)
                                                                                                                                             cgcACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgttt    <  2:1729711/150‑1 (MQ=255)
                                                                                                                                              gcACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgtttg   <  2:3828794/150‑1 (MQ=255)
                                                                                                                                               cacaAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgtttgt  >  1:1085831/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGT  >  CP009273/1865275‑1865567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGT  >  CP009273/1865275‑1865570
                                                                                                                                                   |                                                                                                                                                    
CACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGA                                                                                                                                                    <  SRR19973081.2120093/150‑1 (MQ=60)
   AATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTA                                                                                                                                                 <  SRR19973081.750185/150‑1 (MQ=60)
      CTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCC                                                                                                                                              >  SRR19973081.4483548/1‑150 (MQ=60)
           CAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGA                                                                                                                                         <  SRR19973081.1267806/150‑1 (MQ=60)
            AGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAA                                                                                                                                        >  SRR19973081.238893/1‑150 (MQ=60)
            AGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAA                                                                                                                                        >  SRR19973081.3136169/1‑150 (MQ=60)
            AGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAA                                                                                                                                        >  SRR19973081.4390361/1‑150 (MQ=60)
               TATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTA                                                                                                                                     >  SRR19973081.2557636/1‑150 (MQ=60)
               TATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTA                                                                                                                                     >  SRR19973081.2556931/1‑150 (MQ=60)
                  AACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGG                                                                                                                                  >  SRR19973081.1986672/1‑150 (MQ=60)
                  AACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGGTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGG                                                                                                                                  >  SRR19973081.2519659/1‑150 (MQ=60)
                         ACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAA                                                                                                                           <  SRR19973081.3171551/150‑1 (MQ=60)
                         ACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAA                                                                                                                           <  SRR19973081.3170631/150‑1 (MQ=60)
                         ACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAA                                                                                                                           <  SRR19973081.2016750/150‑1 (MQ=60)
                          CTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTTCGACCTGGGGAATCAACTATAAAAAAATATTGGTTTGTATGTGGGCATTTTTAATGTTATTTATTATAATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAAT                                                                                                                          >  SRR19973081.3778535/1‑150 (MQ=60)
                                TTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATT                                                                                                                    <  SRR19973081.3802674/150‑1 (MQ=60)
                                TTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATT                                                                                                                    <  SRR19973081.2332790/150‑1 (MQ=60)
                                 TTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTA                                                                                                                   <  SRR19973081.921149/150‑1 (MQ=60)
                                  TGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTAT                                                                                                                  <  SRR19973081.629075/150‑1 (MQ=60)
                                   GTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATT                                                                                                                 >  SRR19973081.3534985/1‑150 (MQ=60)
                                   GTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATT                                                                                                                 <  SRR19973081.904656/150‑1 (MQ=60)
                                   GTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATT                                                                                                                 <  SRR19973081.904487/150‑1 (MQ=60)
                                   GTATGTCGTCGATTACTGTCCGGACAACCAGACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATT                                                                                                                 <  SRR19973081.905443/150‑1 (MQ=60)
                                    TATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTA                                                                                                                >  SRR19973081.1323066/1‑150 (MQ=60)
                                      TGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATG                                                                                                              <  SRR19973081.899158/150‑1 (MQ=60)
                                      TGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATG                                                                                                              <  SRR19973081.899558/150‑1 (MQ=60)
                                         CGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCT                                                                                                           <  SRR19973081.4784071/150‑1 (MQ=60)
                                           TCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGG                                                                                                         >  SRR19973081.713572/1‑150 (MQ=60)
                                            CGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGC                                                                                                        >  SRR19973081.4448920/1‑150 (MQ=60)
                                             GATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCA                                                                                                       <  SRR19973081.1567489/150‑1 (MQ=60)
                                             GATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCA                                                                                                       <  SRR19973081.4775287/150‑1 (MQ=60)
                                             GATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCA                                                                                                       <  SRR19973081.1567498/150‑1 (MQ=60)
                                              ATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCAT                                                                                                      <  SRR19973081.3047596/150‑1 (MQ=60)
                                                    GTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAT                                                                                                <  SRR19973081.2420196/150‑1 (MQ=60)
                                                    GTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAT                                                                                                <  SRR19973081.2420218/150‑1 (MQ=60)
                                                    GTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAT                                                                                                <  SRR19973081.2420213/150‑1 (MQ=60)
                                                    GTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAT                                                                                                <  SRR19973081.2420132/150‑1 (MQ=60)
                                                       CGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCT                                                                                             >  SRR19973081.557804/1‑150 (MQ=60)
                                                           CAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAA                                                                                         >  SRR19973081.4269659/1‑150 (MQ=60)
                                                           CAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAA                                                                                         >  SRR19973081.2153709/1‑150 (MQ=60)
                                                           CAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAA                                                                                         >  SRR19973081.2153706/1‑150 (MQ=60)
                                                           CAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAA                                                                                         >  SRR19973081.3667099/1‑150 (MQ=60)
                                                             ACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACT                                                                                       <  SRR19973081.3908566/150‑1 (MQ=60)
                                                              CCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTT                                                                                      <  SRR19973081.1687570/150‑1 (MQ=60)
                                                                   CTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTC                                                                                 >  SRR19973081.622762/1‑150 (MQ=60)
                                                                     GCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTA                                                                               <  SRR19973081.4462300/150‑1 (MQ=60)
                                                                           TGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACT                                                                         >  SRR19973081.3471147/1‑150 (MQ=60)
                                                                           TGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACT                                                                         >  SRR19973081.3204394/1‑150 (MQ=60)
                                                                             GGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCT                                                                       <  SRR19973081.4704959/150‑1 (MQ=60)
                                                                             GGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCT                                                                       <  SRR19973081.1724370/150‑1 (MQ=60)
                                                                             GGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCT                                                                       <  SRR19973081.1724340/150‑1 (MQ=60)
                                                                              GGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTG                                                                      >  SRR19973081.3049242/1‑150 (MQ=60)
                                                                                 ATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGAT                                                                   <  SRR19973081.4303303/150‑1 (MQ=60)
                                                                                 ATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGAT                                                                   <  SRR19973081.4303335/150‑1 (MQ=60)
                                                                                    aacgatacacaactactgggttggcgGTGGGCATTTTTACTGTTCTTGATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAG                                                                <  SRR19973081.3810068/124‑1 (MQ=60)
                                                                                    AACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAG                                                                <  SRR19973081.1386447/150‑1 (MQ=60)
                                                                                    AACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAG                                                                <  SRR19973081.473964/150‑1 (MQ=60)
                                                                                    AACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAG                                                                <  SRR19973081.3810065/150‑1 (MQ=60)
                                                                                    AACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAG                                                                <  SRR19973081.3810066/150‑1 (MQ=60)
                                                                                     ACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGT                                                               <  SRR19973081.1846502/150‑1 (MQ=60)
                                                                                         TACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAA                                                           <  SRR19973081.3180646/150‑1 (MQ=60)
                                                                                            AAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTA                                                        <  SRR19973081.646428/150‑1 (MQ=60)
                                                                                              AAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATA                                                      >  SRR19973081.1774281/1‑150 (MQ=60)
                                                                                                ATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACT                                                    >  SRR19973081.4765523/1‑150 (MQ=60)
                                                                                                ATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACT                                                    >  SRR19973081.4765412/1‑150 (MQ=60)
                                                                                                ATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACT                                                    >  SRR19973081.2315905/1‑150 (MQ=60)
                                                                                                 TATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTA                                                   <  SRR19973081.864587/150‑1 (MQ=60)
                                                                                                 TATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTA                                                   >  SRR19973081.3168452/1‑150 (MQ=60)
                                                                                                   TTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATC                                                 <  SRR19973081.4156524/150‑1 (MQ=60)
                                                                                                   TTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATC                                                 >  SRR19973081.4198317/1‑150 (MQ=60)
                                                                                                     GGTTTTTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCTCATGATTAGCCAGCGAACTATTGCCGTTAATAGCATTATTATGTCTGGCATCGCTTTGCTGCAACTTACTTCTAATGACTCTGGATGAGTATAATTATACTATCTG                                               <  SRR19973081.3896179/150‑1 (MQ=60)
                                                                                                     GGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTG                                               <  SRR19973081.665273/150‑1 (MQ=60)
                                                                                                     GGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTG                                               <  SRR19973081.3896217/150‑1 (MQ=60)
                                                                                                       TTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGT                                             <  SRR19973081.2996845/150‑1 (MQ=60)
                                                                                                           TCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATAT                                         <  SRR19973081.2493008/150‑1 (MQ=60)
                                                                                                           TCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATAT                                         >  SRR19973081.2807367/1‑150 (MQ=60)
                                                                                                                GGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTC                                    <  SRR19973081.2603755/150‑1 (MQ=60)
                                                                                                                GGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTC                                    >  SRR19973081.3999263/1‑150 (MQ=60)
                                                                                                                   CATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCG                                 >  SRR19973081.3196515/1‑150 (MQ=60)
                                                                                                                   CATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCG                                 <  SRR19973081.1425791/150‑1 (MQ=60)
                                                                                                                    ATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGG                                >  SRR19973081.279962/1‑150 (MQ=60)
                                                                                                                      TTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGA                              >  SRR19973081.4266438/1‑150 (MQ=60)
                                                                                                                       TTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGAGAACTATGGCAGTTAATAGCATTATTATTTCTGGCATCGCTATGCTGCAAATTACTTCTACTGACTCTGGATGATTATAATTATACTATCTGGTATATAAGTCGCGGGAT                             <  SRR19973081.989875/150‑1 (MQ=60)
                                                                                                                           CTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAA                         <  SRR19973081.3046225/150‑1 (MQ=60)
                                                                                                                              TTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTT                      <  SRR19973081.2119756/150‑1 (MQ=60)
                                                                                                                               TCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTT                     <  SRR19973081.2765036/150‑1 (MQ=60)
                                                                                                                               TCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTT                     <  SRR19973081.2101514/150‑1 (MQ=60)
                                                                                                                                 TTTATTATCATGCGCACAATATTAGCCAGCGAACTATGTACGTTAATAGAATTATTATGTCTTGCATATCTATGCTGCAAATTAATTCTACTTACTCTGGATGAGTATAATTATACTATATTGTATATAAGTAGCGGGATTtaattttac                   >  SRR19973081.381102/1‑141 (MQ=60)
                                                                                                                                  TTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCA                  <  SRR19973081.1103834/150‑1 (MQ=60)
                                                                                                                                  TTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCA                  <  SRR19973081.1103912/150‑1 (MQ=60)
                                                                                                                                  TTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATG                                                                                               <  SRR19973081.2865725/73‑1 (MQ=60)
                                                                                                                                   TATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAG                 <  SRR19973081.3477913/150‑1 (MQ=60)
                                                                                                                                      TATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTTGCATCGCTATGCTGCAACTTACTTCTACTGACTCTTGATGAGTATAATTATACAATCTGGTATATCAGTCGCGGGAGTGAATTTTCCAGTAA              >  SRR19973081.2901928/1‑150 (MQ=60)
                                                                                                                                      TATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAA              >  SRR19973081.2330674/1‑150 (MQ=60)
                                                                                                                                      TATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTACAGTAA              >  SRR19973081.2901768/1‑150 (MQ=60)
                                                                                                                                       ATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAA             >  SRR19973081.616571/1‑150 (MQ=60)
                                                                                                                                         CATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACT           <  SRR19973081.785172/150‑1 (MQ=60)
                                                                                                                                            GCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTT        <  SRR19973081.4369236/150‑1 (MQ=60)
                                                                                                                                            GCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTT        <  SRR19973081.4369592/150‑1 (MQ=60)
                                                                                                                                               CACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGT     >  SRR19973081.1093027/1‑150 (MQ=60)
                                                                                                                                                 CAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTG   <  SRR19973081.4242226/150‑1 (MQ=60)
                                                                                                                                                 CAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTG   <  SRR19973081.4242271/150‑1 (MQ=60)
                                                                                                                                                 CAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTG   <  SRR19973081.4242288/150‑1 (MQ=60)
                                                                                                                                                  AAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGT  <  SRR19973081.3909219/150‑1 (MQ=60)
                                                                                                                                                  AAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGT  >  SRR19973081.1905876/1‑150 (MQ=60)
                                                                                                                                                  AAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTACAGTAAACTGTTTGTTGT  >  SRR19973081.4800530/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
CACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGT  >  CP009273/1865275‑1865570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑