Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	781,531	T→C	I148T (ATC→ACC)	aroG →	3‑deoxy‑7‑phosphoheptulonate synthase AroG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	781,531	0	T	C	100.0%	132.4 / NA	38	I148T (ATC→ACC)	aroG	3‑deoxy‑7‑phosphoheptulonate synthase AroG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/19);  total (19/19)

TCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGATCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATC  >  NZ_CP009273/781444‑781618                                                                                        |                                                                                        tCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGGCAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCAc                                                                                      >  1:859066/1‑91 (MQ=255) tCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCAc                                                                                      <  1:631153/91‑1 (MQ=255) tCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCAc                                                                                      <  1:45617/91‑1 (MQ=255)   aaCGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCAccc                                                                                    <  1:204171/91‑1 (MQ=255)   aaCGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCAccc                                                                                    <  1:508276/91‑1 (MQ=255)    acgacgGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCAcccc                                                                                   >  2:731877/1‑91 (MQ=255)      gacgGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCcac                                                                                 <  2:42616/91‑1 (MQ=255)         ggTCTGCGTATAGCCCGTAACTTGCTGCTTGATATTAACGCCCGCGGTCTGCCAGCGGCAGGGGAGTTTCTCGATATGACCACCCCACAat                                                                              >  2:1070381/1‑91 (MQ=255)         ggTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGGGAGTTTCTCGATATGACCACCCCACAat                                                                              >  1:314500/1‑91 (MQ=255)          gTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGGGAGTTTCTCGATATGACCACCCCACAata                                                                             >  2:489956/1‑91 (MQ=255)              gCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATAtctc                                                                         >  1:480901/1‑91 (MQ=255)                gTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGc                                                                       <  1:275367/91‑1 (MQ=255)                    aGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTgcc                                                                   >  1:926473/1‑89 (MQ=255)                        cGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCtga                                                               >  1:505515/1‑91 (MQ=255)                        cGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCtga                                                               >  2:1045236/1‑91 (MQ=255)                         gTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCtgat                                                              >  1:753216/1‑91 (MQ=255)                         gTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGGGAGTTTTTCGATATGACCACCCCCCAAAATCTCGCTGACCtgat                                                              >  2:687174/1‑91 (MQ=255)                          tAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCtgatg                                                             <  2:265116/91‑1 (MQ=255)                             aTTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGc                                                          <  1:128235/91‑1 (MQ=255)                              ttGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCt                                                         <  1:772031/91‑1 (MQ=255)                                   gctTGATATTAACGACAGCGGTCTGCCAGCGGCAGGGGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGgc                                                    >  2:562515/1‑91 (MQ=255)                                     ttGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGgcgc                                                  <  1:562515/91‑1 (MQ=255)                                         tatTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAAtt                                              <  1:852178/91‑1 (MQ=255)                                               cGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCa                                        <  2:963736/91‑1 (MQ=255)                                                gACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCAc                                       <  2:924337/91‑1 (MQ=255)                                                  cAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGt                                     >  2:493385/1‑91 (MQ=255)                                                  cAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGt                                     >  1:392519/1‑91 (MQ=255)                                                  cAGCGGTCTGCCAGCGGCAGGGGAGTTTTTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGGGCAATTGGCGCACGt                                     >  2:6068/1‑91 (MQ=255)                                                  cAGCGGTCTGCCAGCGGCAGGGGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGt                                     >  2:870434/1‑91 (MQ=255)                                                      ggTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTacca                                 >  2:233535/1‑91 (MQ=255)                                                      ggTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTacca                                 >  1:770183/1‑91 (MQ=255)                                                       gTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTaccac                                >  1:944297/1‑91 (MQ=255)                                                        tCTTCCAGCGGCAAGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTaccacc                               <  2:923182/91‑1 (MQ=255)                                                        tCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTaccacc                               <  2:103066/91‑1 (MQ=255)                                                         cTGCCAGCGGCAGGTGGGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCg                              >  2:214061/1‑91 (MQ=255)                                                             cAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATc                          <  2:1021647/91‑1 (MQ=255)                                                                            ttCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGa           >  1:511296/1‑91 (MQ=255)                                                                                cGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTg       <  2:480901/91‑1 (MQ=255)                                                                                    atGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCATGTGCACCGCGAACTGGCAt   <  1:1088395/91‑1 (MQ=255)                                                                                     tGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATc  <  2:759665/91‑1 (MQ=255)                                                                                        |                                                                                        TCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGATCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATC  >  NZ_CP009273/781444‑781618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑