Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,260,284	A→G	D372G (GAC→GGC)	hemA →	glutamyl‑tRNA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,260,284	0	A	G	100.0%	25.3 / NA	10	D372G (GAC→GGC)	hemA	glutamyl‑tRNA reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/4);  total (6/4)

CATTCGCGAGTATCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAG‑CGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCG  >  NZ_CP009273/1260201‑1260367                                                                                     |                                                                                    cATTCGCGAGTATCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAG‑CGTTGGCGGCCCTTGAGCAGGGCGGCGGCGCGaaa                                                                              <  2:71607/91‑1 (MQ=255)          gTATCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAG‑CGTTAGCGGCCCTTGAGCAGGGCGGCGGCGCGCAAGCCATTATg                                                                     >  1:169424/1‑91 (MQ=255)            aTCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAG‑CGTTAGCGGCCCTTGAGCAGGGCGGCGGGGCGCAAGCCATTATGCa                                                                   >  1:590334/1‑91 (MQ=255)             ccGCAGCCAGGAGGAGAAAGTCGGGAATGAGTAACCCGCAAAAG‑CGTTAGCGCCCTTGGAGGGGGGCGGGGGCGCAAAAGCCATTATGCAg                                                                  <  2:950291/90‑1 (MQ=255)                     aGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAG‑CGTTAGCGGCCCTTGAGCAGGGCGGCGGCGCGCAAGCCATTATGCAGGATCTGGc                                                          >  1:540476/1‑91 (MQ=255)                      ggCGGAGGATGTTCGTGATGAGTTAACCGAAAAGG‑TGTTGGCGGCCCTTGAGGGGGGCGGGGGCGCGAAAGCCATTATGCAGGATCTGGCa                                                         <  2:592696/91‑1 (MQ=255)                                   cgcgATGAGTTACCCCCCAAAG‑CGTTAGCGGCCCTTGAGCAGGGCGGCGGCGCGAAAGCCATTATGCAGGATCTGGCATGGAAACTGACTa                                            <  1:147215/91‑1 (MQ=255)                                    gcgATGAGTTAACCGCCAAAG‑CGTTAGCGGCCCTTGAGCAGGGCGGCGGCGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTaa                                           >  1:790379/1‑91 (MQ=255)                                    gagatgatTAACCCGCAAAAG‑CGTTAGCGCCCCTGGAGCGGGGGGGCGGCGCAAAAGCCATTATGCAGGATCTGGCATGGAAACTGACTaa                                           <  2:995400/89‑1 (MQ=255)                                                accccAAAGCCGTTACCGGCCGTTGCGCAGGGCGGCGGGGCCCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATcc                                <  1:996508/88‑1 (MQ=255)                                                   ccAAAG‑CGTTAGCGGCCCTTGAGCAGGGCGGCGGCGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATgc                            >  1:528558/1‑91 (MQ=255)                                                   ccAAAG‑CGTTAGCGGCCCTTGAGCAGGGCGGCGGCGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATgc                            >  2:123470/1‑91 (MQ=255)                                                     aaaG‑CGTTAGCGGCCCTTGAGCAGGGCGGCGGCGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATgcgc                          >  2:932710/1‑91 (MQ=255)                                                              ggCGGCCCTGGGGCGGGGCGGCGGCACAAAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGaaa                  <  2:244792/90‑1 (MQ=255)                                                                              ggcggcggcGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGgccg  >  1:408760/1‑91 (MQ=255)                                                                                     |                                                                                    CATTCGCGAGTATCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAG‑CGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCG  >  NZ_CP009273/1260201‑1260367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑