Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,896,621	A→G	D147D (GAT→GAC)	bglB ←	6‑phospho‑beta‑glucosidase BglB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,896,621	0	A	G	100.0%	82.5 / NA	25	D147D (GAT→GAC)	bglB	6‑phospho‑beta‑glucosidase BglB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/6);  total (19/6)

TGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAG  >  NZ_CP009273/3896539‑3896701                                                                                   |                                                                                  tGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGa                                                                            >  1:34273/1‑90 (MQ=255)     gATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑c                                                                       <  2:570307/90‑1 (MQ=255)      aTTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑Ct                                                                      <  2:11845/90‑1 (MQ=255)       tttCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑CTc                                                                     >  1:261945/1‑90 (MQ=255)         tCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑CTCGa                                                                   >  2:487133/1‑90 (MQ=255)                                 aCTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTc                                           >  1:566713/1‑90 (MQ=255)                                        gTTGGTAGGGAGAAAAGCCCGGGGGGGCGTAATGCTCGAAGGGGTCGATGCCCG‑CTCGATTACCCAAACCGCCGTAGTTTTTCACCAGcc                                    <  1:96867/90‑1 (MQ=255)                                           ggTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCat                                 >  2:505103/1‑90 (MQ=255)                                           ggTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCat                                 >  1:551186/1‑90 (MQ=255)                                            gTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCata                                >  2:307468/1‑90 (MQ=255)                                            gTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCata                                >  2:194409/1‑90 (MQ=255)                                                         cgcGTGCGGGCGTAATGCGCGAAGTGGTCGA‑GAGCGCCTCCATGCCCCCACCCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTaa                   <  2:280225/88‑1 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCAttt                        >  1:141278/1‑82 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCAttt                        <  2:141278/82‑1 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                >  2:211432/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                >  2:21684/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                >  2:230872/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                >  1:509149/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                >  1:490375/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                >  1:117197/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                >  2:74776/1‑90 (MQ=255)                                                                  gCGTAATGCTCGAAGTGGTCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAAc          >  1:41345/1‑90 (MQ=255)                                                                          cTCGAAGTGGTCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAg  >  2:163466/1‑90 (MQ=255)                                                                          cTCGAAGTGGTCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAg  >  2:296532/1‑90 (MQ=255)                                                                          cTCGAAGGGGTCGATGCCCG‑CTCTATTACCCAAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAg  <  1:174792/90‑1 (MQ=255)                                                                                   |                                                                                  TGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCG‑CTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAG  >  NZ_CP009273/3896539‑3896701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑