Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	264,689	(A)5→4	intergenic (‑18/+311)	ykfA ← / ← perR	GTPase family protein/LysR substrate‑binding domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	264,685	0	A	.	100.0%	127.0 / NA	31	intergenic (‑14/+315)	ykfA/perR	GTPase family protein/LysR substrate‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (13/18);  total (13/18)

CCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTT  >  NZ_CP009273/264600‑264769                                                                                      |                                                                                     ccGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGaaaat                                                                                  <  2:304137/90‑2 (MQ=255)  cGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGGAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGaaaata                                                                                 >  1:733553/1‑88 (MQ=255)         aTACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATaaaaa                                                                         <  2:317736/90‑1 (MQ=255)                ttGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAg                                                                  >  1:207161/1‑90 (MQ=255)                ttGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAg                                                                  >  2:248731/1‑90 (MQ=255)                ttGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAg                                                                  >  1:745024/1‑90 (MQ=255)                  gCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGaa                                                                >  1:489676/1‑90 (MQ=255)                             gCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTc                                                     <  2:358966/90‑1 (MQ=255)                               aaGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTccg                                                   <  2:43415/90‑1 (MQ=255)                                        cTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAAtt                                          <  2:498274/90‑1 (MQ=255)                                        cTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAAtt                                          <  2:745024/90‑1 (MQ=255)                                            aaCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGt                                      <  2:732351/90‑1 (MQ=255)                                             aCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTa                                     <  2:13678/90‑1 (MQ=255)                                                  ttCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTc                                <  1:516091/90‑1 (MQ=255)                                                  ttCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTc                                <  2:572883/90‑1 (MQ=255)                                                    cAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCag                              <  2:554001/90‑1 (MQ=255)                                                      aaCCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCagag                            <  1:674557/90‑1 (MQ=255)                                                          ttCAGAATTATTCATCTCGTCTCCTCTG‑CAACTAAAAAAAAGTGCAAGAATCATGACTTTTAGCCGATAATTGCGTATGTTCAGAGtgct                        <  1:554696/90‑3 (MQ=255)                                                          ttCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgat                        <  1:385216/90‑1 (MQ=255)                                                          ttCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgat                        <  1:248731/90‑1 (MQ=255)                                                          ttCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgat                        <  2:85704/90‑1 (MQ=255)                                                           tCAGAATTATTCATCACTACTCCTCTG‑AAAATAATaaaaa                                                                         <  1:150862/40‑1 (MQ=37)                                                           tCAGAATTATTCATCACTACTCCTCTG‑AAAATAATaaaaa                                                                         >  2:150862/1‑40 (MQ=25)                                                           tCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgatg                       >  2:200592/1‑90 (MQ=255)                                                               aaTTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGatat                   <  2:415063/90‑1 (MQ=255)                                                                  tattCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGatatata                >  1:1076/1‑90 (MQ=255)                                                                   attCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGatatatat               >  2:260087/1‑90 (MQ=255)                                                                        tcaCTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGaa          >  2:178515/1‑90 (MQ=255)                                                                             actCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAAtt                                          >  2:212687/1‑53 (MQ=255)                                                                             actCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAAtt                                          <  1:212687/53‑1 (MQ=255)                                                                             actCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGAttt     >  1:187886/1‑90 (MQ=255)                                                                                cctcTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGAtttttt  >  2:448565/1‑90 (MQ=255)                                                                                cctcTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGAtttttt  >  2:138951/1‑90 (MQ=255)                                                                                      |                                                                                     CCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTT  >  NZ_CP009273/264600‑264769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑