| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,934,995 | +C | intergenic (‑308/‑173) | yieP ← / → BW25113_RS19520 | FadR/GntR family transcriptional regulator/16S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,934,991 | 1 | . | C | 86.2% | 73.6 / 7.7 | 29 | intergenic (‑304/‑177) | yieP/BW25113_RS19520 | FadR/GntR family transcriptional regulator/16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base . (1/3); new base C (10/15); total (11/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
CCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCA‑C‑A‑CCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGAT > NZ_CP009273/3934902‑3935075 | ccgccgGGTCAGCGGGGTTCTCCTGAGAACTCCGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGca‑c‑a > 2:764148/1‑90 (MQ=33) tGAGAACTCCGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAaaagc < 2:736933/90‑1 (MQ=255) aCTCCGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAaaagcaaagc < 1:508199/90‑1 (MQ=255) aCTCCGGCAGAGAAAGCAAAAATAAATGCTTGACCCTGTAGCGGGAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAaaagcaaagc < 2:504092/90‑1 (MQ=11) ccGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCA‑CAA‑CCCCGCGCCGCTGAGAAAAAGCAAAGCGGc > 1:339002/1‑90 (MQ=255) ccGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGc > 1:361678/1‑90 (MQ=255) gCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACt < 2:473355/90‑1 (MQ=255) gCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACt < 2:164336/90‑1 (MQ=255) cAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAAc < 1:631795/90‑1 (MQ=255) aaaaTAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACC‑C‑CCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACaa < 1:699790/90‑1 (MQ=11) aaaaTAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACaa < 2:361678/90‑1 (MQ=255) aaaaTAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACaa < 2:339002/90‑1 (MQ=255) taaatGCTTGACTCTGTAGCGGGAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTa < 1:604763/90‑1 (MQ=255) aatGCTTGACTCTGTAGCGGGAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATc > 1:704536/1‑90 (MQ=255) tGCTTGACTCTGTAGCGGGAAAGCGTATTATGCA‑CGC‑CCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAg < 2:244982/90‑1 (MQ=11) cTTGACTCTGTAGCGGGAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGAc < 1:759506/90‑1 (MQ=255) tGACTCTGTAGCGGGAAAGCGTATTATGCACC‑C‑CCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACaa < 1:432136/90‑1 (MQ=11) ctctGTAGCTGGAAAGCGTATTATGCA‑C‑ACCCCCCCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCt < 1:402017/90‑1 (MQ=11) tctGTAGCGGGAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCtg > 2:310514/1‑90 (MQ=255) gTAGCGGGAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCtgtgt > 1:10915/1‑90 (MQ=255) gTAGCGGGAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCtgtgt > 2:285542/1‑90 (MQ=255) cGGGAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGc < 1:457423/90‑1 (MQ=255) cGGGAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGc < 2:270231/90‑1 (MQ=255) gggAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACt < 2:254748/54‑1 (MQ=14) gggAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACt > 1:254748/1‑54 (MQ=14) gggAAAGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCa > 1:358262/1‑90 (MQ=255) aGCGTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGa > 2:240048/1‑90 (MQ=255) gTATTATGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGa > 1:148477/1‑90 (MQ=255) ttatGCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGAGGGCACTCGAAGATAc < 1:403166/90‑1 (MQ=11) gCA‑C‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGAt > 2:350374/1‑90 (MQ=255) | CCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCA‑C‑A‑CCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGAT > NZ_CP009273/3934902‑3935075 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |