Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	977,631	A→C	I377L (ATC→CTC)	ldtD →	L,D‑transpeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	977,631	0	A	C	100.0%	25.3 / NA	9	I377L (ATC→CTC)	ldtD	L,D‑transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (7/2);  total (7/2)

AACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGG  >  NZ_CP009273/977552‑977710                                                                                |                                                                                aaCGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGCTCATGGTTaa                                                                       >  1:212401/1‑90 (MQ=255) aaCGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGCTCATGGTTaa                                                                       >  2:33417/1‑90 (MQ=255)  aCGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGCTCATGGTTAAc                                                                      >  1:56762/1‑90 (MQ=255)         cccAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGCTCATGGTTAACATTCCgg                                                               <  1:322598/90‑1 (MQ=255)               gTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGCTCATGGTTAACATTCCGGCCTAtt                                                         >  2:305427/1‑90 (MQ=255)                   tGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGCTCATGGTTAACATTCCGGCCTATTCGCt                                                     >  1:281774/1‑90 (MQ=255)                        tgtTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGCTCATGGTTAACATTCCGGCCTATTCGCTGGTct                                                <  1:305427/90‑1 (MQ=255)                                        ccAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGCTCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCaa                                >  1:57544/1‑90 (MQ=255)                                                                      ttCTACCGGGCTCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTgtcgg  >  2:289418/1‑90 (MQ=255)                                                                                |                                                                                AACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGG  >  NZ_CP009273/977552‑977710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑