New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3154233 =	36 (1.040)	5 (0.160)	5/236	2.9	13.7%	intergenic (+11/+29)	yghB/yqhC	general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter/transcriptional activator of yqhD
	?	NC_000913	3154259 =	30 (0.940)					intergenic (+37/+3)	yghB/yqhC	general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter/transcriptional activator of yqhD
Rejected: Frequency below/above cutoff threshold.

CCAGCGCAGCAGCGATGCGCGTCGGCTATGAAAGCGCATCGCAATTTAGCCGTGAGTTTAAACGTTACTTCGGTGTGACGCCGGGGGAAGATGCGGCAAGAATGCGGGCGATGCAGGGGAATTAAATACGTTCCCGGTACAGCAACCGGGAACG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3154386‑3154233 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑accgggaacgTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATG  >  NC_000913/3154259‑3154402                                                                                                                                                                                                                                                                                                            CCAGCGCAGCAGCGATGCGCGTCGGCTATGAAAGCGCATCGCAATTTAGCCGTGAGTTTAAACGTTACTTCGGTGTGACGCCGGGGGAAGATGCGGCAAGAATGCGGGCGATGCAGGGGAATTAAATACGTTCCCGGTACAGCAACCGG                                                                                                                                                       <  1:171601/149‑1                                                                                                                 GCAGGGGAATTAAATACGTTCCCGGTACAGCAACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGC                                                          <  3:1494/130‑1                                                                                                                 GCAGGGGAATTAAATACGTTCCCGGTACAGCAACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGC                                                          >  4:1494/1‑130                                                                                                                     GGGAATTAAATACGTTCCCGGTACAGCAACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTC                                               >  1:163445/1‑137                                                                                                                     GGGAATTAAATACGTTCCCGGTACAGCAACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTC                                               <  2:163445/137‑1                                                                                                                                  GTTCCCGGTACAGCAACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCG                      >  5:59532/1‑149                                                                                                                                                 ACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCG       >  1:123951/1‑149                                                                                                                                                     GGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCAT   <  1:65321/149‑1                                                                                                                                                     GGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCAT   >  2:65321/1‑149                                                                                                                                                      GAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATG  >  1:136090/1‑149                                                                                                                                                                                                                                                                                                            CCAGCGCAGCAGCGATGCGCGTCGGCTATGAAAGCGCATCGCAATTTAGCCGTGAGTTTAAACGTTACTTCGGTGTGACGCCGGGGGAAGATGCGGCAAGAATGCGGGCGATGCAGGGGAATTAAATACGTTCCCGGTACAGCAACCGGGAACG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3154386‑3154233 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑accgggaacgTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATG  >  NC_000913/3154259‑3154402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.