Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	1,326,761	+G	intergenic (+62/‑108)	h16_B1172 → / → h16_B1173	Amidase/MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	1,326,760	1	.	G	100.0%	122.4 / NA	32	intergenic (+61/‑109)	h16_B1172/h16_B1173	Amidase/MFS transporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (18/14);  total (18/14)

GCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCC‑GACAC‑GCGGCGACAA‑GTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGA  >  AM260480/1326634‑1326873                                                                                                                                  |                                                                                                                  gCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAA‑ggtcg                                                                                                              >  2:310752/1‑130 (MQ=255)      tGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGa                                                                                                           >  1:1063029/1‑133 (MQ=255)      tGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGa                                                                                                           >  1:516415/1‑133 (MQ=255)       gCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACa                                                                                                         >  1:780576/1‑134 (MQ=255)           ccGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAgc                                                                                                     <  2:329583/134‑1 (MQ=255)                   gCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAAc                                                                                             <  1:310752/134‑1 (MQ=255)                        cggcgcgcgcCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCg                                                                                         >  2:1159743/3‑133 (MQ=255)                             cgcgcCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAAc                                                                                     >  1:854505/1‑132 (MQ=255)                              gcgcCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACgag                                                                                  <  2:854505/134‑1 (MQ=255)                                 ccggccgCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACgaggag                                                                               >  1:100290/1‑134 (MQ=255)                                       gccgcATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAgcg                                                                                                    <  1:258549/107‑1 (MQ=255)                                       gccgcATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAgcg                                                                                                    >  2:258549/1‑107 (MQ=255)                                           cATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGa                                                                     <  2:780576/134‑1 (MQ=255)                                                                      cgcgcCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGc                                          >  2:992855/1‑134 (MQ=255)                                                                       gcgcCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGcc                                         >  2:571303/1‑134 (MQ=255)                                                                        cgcCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCg                                        <  1:1159743/134‑1 (MQ=255)                                                                          ccGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACgagga                                                                                <  1:384366/92‑1 (MQ=255)                                                                          ccGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACgagga                                                                                >  2:384366/1‑92 (MQ=255)                                                                           cGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGa                                                                     >  2:235203/1‑102 (MQ=255)                                                                           cGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGa                                                                     <  1:235203/102‑1 (MQ=255)                                                                            ggAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAAtt                                    >  2:210580/1‑134 (MQ=255)                                                                                     ttttATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCAcagc                           <  1:992855/134‑1 (MQ=255)                                                                                       ttATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGgcg                                                               >  2:554905/1‑96 (MQ=255)                                                                                       ttATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGgcg                                                               <  1:554905/96‑1 (MQ=255)                                                                                                ctctACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGc                  <  2:516415/132‑1 (MQ=255)                                                                                                ctctACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTc                <  1:210580/134‑1 (MQ=255)                                                                                                 tctACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCAcag                            <  2:484995/121‑1 (MQ=255)                                                                                                 tctACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCAcag                            >  1:484995/1‑121 (MQ=255)                                                                                                 tctACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTc                >  2:392163/1‑133 (MQ=255)                                                                                                 tctACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTc                >  2:653165/1‑133 (MQ=255)                                                                                                       aaCGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCggg         >  1:124246/1‑134 (MQ=255)                                                                                                          gcccgccgGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCa      <  1:392163/134‑1 (MQ=255)                                                                                                              gccggACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCatgga  >  2:293520/5‑134 (MQ=255)                                                                                                                                  |                                                                                                                  GCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCC‑GACAC‑GCGGCGACAA‑GTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGA  >  AM260480/1326634‑1326873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑