Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM14,344,3120GA36.9% 45.0 / 38.9 109intergenic (+43/‑113)PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)–/–
Reads supporting (aligned to +/- strand):  ref base G (40/13);  new base A (31/0);  total (96/13)
Fisher's exact test for biased strand distribution p-value = 1.54e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 161 total aligned reads displayed.

CGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTG  >  AE015451_phaM1/4344176‑4344448
                                                                                                                                         |                                                                                                                                          
cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgcccg                                                                                                                                            <  2:1080602/137‑1 (MQ=255)
   cAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGAACTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTTCCCGATTGAGCACCGTCcacgc                                                                                                                                         >  2:16780029/1‑137 (MQ=255)
    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                        <  2:10273001/137‑1 (MQ=255)
    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                        <  2:9243030/137‑1 (MQ=255)
    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                        <  2:2162206/137‑1 (MQ=255)
    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                        <  1:1853623/137‑1 (MQ=255)
    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                        <  2:15012269/137‑1 (MQ=255)
    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                        <  2:4956863/137‑1 (MQ=255)
    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                        <  1:14775573/137‑1 (MQ=255)
        tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGcac                                                                                                                                    <  1:10613764/137‑1 (MQ=255)
          aaGGTGCGTTGTGCAGTGGCAGACGCTTGCATTTATACCAAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTGGTACGTTGCAGC‑GCCTGCCAGATTGAGCACCGCCCGCGCGGCacac                                                                                                                                  <  1:13282299/137‑1 (MQ=255)
          aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                                                  <  1:12047586/137‑1 (MQ=255)
          aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                                                  <  1:13282342/137‑1 (MQ=255)
           aGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGCCAAACCGAGCACCACCCACACGGAacaca                                                                                                                                 >  1:5656936/1‑136 (MQ=255)
                 gTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GACTGCCAAATCTAGCACCGCCCCCGCAGGACACAAtattg                                                                                                                           >  1:16567702/1‑133 (MQ=255)
                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGCCAGATCGAGAACCGCCCGCGCGGTACACGATC‑CGGCa                                                                                                                        >  1:2759198/1‑137 (MQ=255)
                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCCGATCGAGCACCACCCGCGCGGCACAAGATC‑TGGCa                                                                                                                        >  2:2860739/1‑137 (MQ=255)
                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAACAACCACGCGGAACAAGATC‑TGGCa                                                                                                                        >  1:2103621/1‑137 (MQ=255)
                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCTCCAAATCCAGCACCACCCACACGGCACAAGATC‑TGGCa                                                                                                                        >  2:16389959/1‑137 (MQ=255)
                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCAGATCGAGCACCACCCGCGCGGCACAAGAGC‑TGGCa                                                                                                                        >  2:9390942/1‑137 (MQ=255)
                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCCGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAAAACCACCCACGCGGAACACAACC‑CGGCa                                                                                                                        >  2:3343373/1‑137 (MQ=255)
                        aGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGTCCAATCGAGAACCGCCCCCGCGGCACACGACC‑TTGCaagagc                                                                                                                   >  2:8583864/1‑132 (MQ=255)
                          tGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCCAATCGAGCACCGCCCGCGCAGCACACGATC‑TGGAAAGAGCTg                                                                                                                 >  2:1371700/1‑137 (MQ=255)
                           ggCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGT‑GCCCGCCAGAGCGAGAACCACCCGCAAGGAACACGAGC‑TGGGAGGCGCTG‑c                                                                                                               >  2:17712853/1‑137 (MQ=255)
                           ggCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGATGCCCGAGCGAGCACCACCCACACGGCACAAAACC‑TGGCAGGCGCTG‑c                                                                                                               >  1:12080752/1‑137 (MQ=255)
                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTTCCAAATCGAGCACCCCCCCCACGGCACACGATC‑TGGCAGGCGCTG‑TAc                                                                                                             >  1:18050650/1‑137 (MQ=255)
                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCTCGCACCACCCACACCGCCCACAACC‑TGGAAAGCGACG‑CAc                                                                                                             >  1:5598062/1‑137 (MQ=255)
                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAACACCGCCCACGCGGCACACGATC‑TGGCAAGAGCTG‑CAc                                                                                                             >  2:15542562/1‑137 (MQ=255)
                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGACCGAACACCACCCAAACGGCAAACAATC‑TCGCCAGCGCTG‑CAc                                                                                                             >  2:12460380/1‑137 (MQ=255)
                              aGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGTGGCACACGATC‑TGGACGGCGATG‑AAcc                                                                                                            >  2:4166103/1‑136 (MQ=255)
                              aGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCACCCACGTAGCACAAGATC‑TCGCCGGCGCTG‑AACt                                                                                                            >  2:12233566/1‑137 (MQ=255)
                              aGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCCAATCGAGCACCGCCCACGCGGCACACGACC‑TGGCAGGCGCTG‑CACt                                                                                                            >  2:12233600/1‑137 (MQ=255)
                              aGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGACCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GACTGCCAGATCGAGAACCACCCGCGTGGCAACCGATC‑TGGCAGGAGCTgtccc                                                                                                             >  2:4166104/1‑133 (MQ=255)
                                  gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTTCCAGATCAAACACCGCCCGCTCTGCACACAATC‑TGGCAGGTGCTT‑CCCTACTg                                                                                                        >  1:7571838/1‑137 (MQ=255)
                                  gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGTCCGAGCGAGCACCACCCCCGCGGAACACAATC‑TGGCAGGGGATG‑CAATACTg                                                                                                        >  1:6568811/1‑137 (MQ=255)
                                  gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGCCCAACCTAACCCCACCCCCGCCGCACACAACC‑TTGCACGCGCCG‑CAcgcccg                                                                                                        >  1:12203579/1‑132 (MQ=255)
                                       cATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTTCCCGATCGAGCACCGCCCGCGCGGCACACGATC‑TGGGCGGAGCTG‑CCCTATTTGAGGc                                                                                                   >  2:14263156/1‑137 (MQ=255)
                                         tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTTCCAGATCGAGCCCCCCCCGCCCGGTCCACGACC‑CTGCAAGAGCTG‑CACCTCTGGAGGCGt                                                                                                 >  1:6925424/1‑137 (MQ=255)
                                                cGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAAAACCGACCGCGCGGCACAAGATC‑TGGCAGGGGCTG‑CAATATTGGAGGCATACGcccc                                                                                          >  2:11446671/1‑137 (MQ=255)
                                                 gAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCCGCCAAAGCAAAAACCACCCACACAGCACACGACC‑TTGCAGGAGATG‑CACCCTTTGGGGAGTACAccccc                                                                                         >  1:13190603/1‑136 (MQ=255)
                                                   aTGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCACGTGGCACCCGATC‑TTGCCGGTGCTG‑CACTATTTGCGGAGTACGCCCCGTc                                                                                       >  2:12800576/1‑137 (MQ=255)
                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCCTCCCA‑AGCGAGAACCGCCCGCGCGGAAAACGATCTTGTCAGG‑GTTG‑CACTCTTTGAGGAGTCCTCCCCATcac                                                                                     >  1:9364770/1‑137 (MQ=255)
                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTTTCCGATTTAGCACCCCCCCCGCTGCCCCCAATC‑CTGCCAGCGCTG‑TACTCTTTTAGGCGTCCACCCCGTccc                                                                                     >  2:15653472/1‑135 (MQ=255)
                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTGCCCAATCGAGGACAACACACGCGGAACACAAGG‑TGGGAGGTGCGG‑GAATAGTGGGGGGGTGCGCACCGGcac                                                                                     >  2:5622965/1‑137 (MQ=255)
                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCCTTCAGGACGAACACCCCCCACGCGGCACACAAAC‑TGGCAGGAACTGCCACCTTTTG‑GGCGTACCCCCCGTccc                                                                                     >  2:5264365/1‑135 (MQ=255)
                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCCGCCAGACCAAACACCGCCCCCGCGGCAAACAAGC‑TTGCCGGAGATG‑CACTCTTTGAGGCGTACGCCCCGTcaa                                                                                     >  2:3524877/1‑136 (MQ=255)
                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTTTCCGATCTCGCCCCCCCCCCGCCGCACACGACC‑TTGCCGGCGCTG‑CACCCTTTGAGGCGTACACCCCCTccc                                                                                     >  2:17664556/1‑135 (MQ=255)
                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGTCCGACCTAACACCACCCACACGGCACACAACC‑TTGCCAGCGCTG‑CACTCCTTGAGGCGTCCCCCCCATccc                                                                                     >  2:7053648/1‑135 (MQ=255)
                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGTCCAAGCTAGCACCCCCCCCACAGCACCCAAGC‑TGGCAAGCGATT‑TACCCTTTGAGGCGTACGCCCCCTccc                                                                                     >  1:17501529/1‑135 (MQ=255)
                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGCCCGACCAAACACCACCCACACAGCACAAAACC‑CAGCAGGAGATG‑CACTACTTGAGGAGTACGCCCCCTcac                                                                                     >  1:12526561/1‑137 (MQ=255)
                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGCCAGAGCGAGAACCCCCCACCTAGCAAACAACC‑CTGCAAGAGCTG‑CACTCTTGGAGGCGGACGCCCCATcac                                                                                     >  1:10079404/1‑137 (MQ=255)
                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCCGCCCGATCGAGCACCGCCCCCGCGGCACACAACC‑TTGTAGGCGCTT‑CACCATTTGAGGAGTACGCCCCTTcac                                                                                     >  2:3411010/1‑137 (MQ=255)
                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCCGCCAAAGCGAAAACCCCCCCCCCGGCACCCAACC‑CTGCAAGCGCTT‑CCCTCCTTGAGGAGTACACCCCCTccc                                                                                     >  1:4151860/1‑135 (MQ=255)
                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCCGAACACGGCC‑CGGCCGGCGCTG‑TCATATTTGAGGCCTACACCCCATcca                                                                                     >  2:4678960/1‑135 (MQ=255)
                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GACTGCCCGATCGAGCACCGCCCGCGCGGAAAAAAATC‑TTGCAGGCGCTG‑CACCCTTGGAGGCGTACACCCCGTcac                                                                                     >  2:8998455/1‑137 (MQ=255)
                                                       gCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCGTTCCAAATCTAGACCCACCCACGCGGCACAAAAAC‑TGGCAGGCGCTG‑GCCCAATGGAGGCGTAAACCCCATccccc                                                                                   >  2:16499967/1‑133 (MQ=255)
                                                         cGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCCGCCCGAGCGAGAACCACCCCCACGGAACACGACC‑CGGTAGGAGATG‑CAATCTTGGAGGCGTACGCCCCCTcccactc                                                                                 >  2:9691790/1‑134 (MQ=255)
                                                          gagaGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCCAACCGAACCCCCCCCCCCCCGCACCCCACC‑TCGCCAGCGCTT‑CACCCCTTGAGGCGTACACCCCCTcaccctcc                                                                                >  2:1611365/1‑132 (MQ=255)
                                                                    tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTTCCAGATCTAGCACCGCCCACGCGGCACACGATC‑TGGCAGGCGCTG‑ACCTCTTGGAGGCGTACACCCCGGCACATTACCATCccacca                                                                      >  2:8150211/1‑137 (MQ=255)
                                                                    tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGCACAAGGTT‑TGGAAGGCGTTG‑TACTACTTGAGGCGTCCGACCCATCACATTGTTATCCCgacc                                                                      >  2:17528641/1‑134 (MQ=255)
                                                                    tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCAAACCGAGCACCACCCCCGCGGCACACAATC‑CGGCCGGCGCTG‑CAACATTGGAGGCATAAGCCCCATCACATTACCATCAcaaca                                                                      >  2:8964918/1‑133 (MQ=255)
                                                                         aaGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTTCCCGATCCAACACCCCCCGCGCAGCACCCAATC‑TGGCAAGCACTT‑TACCACTTGAAGCGTACACCCCATAAAAGTATCACCACGCCACTGTc                                                                 >  2:7707491/1‑137 (MQ=255)
                                                                         aaGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATTGAGCACCGCCCGCGCGGCACACGATC‑TGGCAGGCGCTG‑CACCATTGGAGGAGTACGCCCCATCACACTCTCATCACGCCACCGTc                                                                 >  2:8380474/1‑137 (MQ=255)
                                                                                   gggCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTTCCAGATCGAGCACCGCCCGCGCGGAACACAATC‑TGGCAGGAGATG‑CAACATTGGAGGCGTACGCCCCGTCACATTATCATCCCACCACTTTCGTAACCCGcc                                                       >  2:17238295/1‑137 (MQ=255)
                                                                                                ctctTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATA‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATg                                          <  2:14760570/137‑1 (MQ=255)
                                                                                                              aGC‑GGCTGCCCAATCGCGCACCGCCCACGCGGCACACAACC‑TGGCAGAAGACG‑CACCATTGGAGGCGTACGCCCCATCCCATTATCATCAAGCCACCGGCGTAATCTTCCGGGAAACTCTCTGCCACAGGTCACACg                            >  2:13149265/1‑137 (MQ=255)
                                                                                                              aGC‑GCCTGCCCAATCTAGCCCCCCCCGCTCCGCACCCAACC‑TGGCAGGCGCTG‑CACTCTTTGAGGCGTCCGCCCCCCCACATTCTCATCCCGCCACCGTCGTAATCTGCCGGCCAACCCTATGCCACCAGTCAACCg                            >  2:5682922/1‑137 (MQ=255)
                                                                                                              aGC‑GCCTGCCAGATCGAGCACCGTCCGCGCGGCACACGAGC‑TGGGCAGTGCTG‑CACTATTTGAGGCGTACGCCCCATCACACTGTCATCACACCCCTGTTGTCATCCTCCGGCCCACTCTCTGCCAAAGGTCACCCg                            >  2:11366027/1‑137 (MQ=255)
                                                                                                              aGC‑GCCTGCCAGATCGAGCACCGCCCACGCGGAACACAAAC‑TGGAAAGCGCTG‑CCCTCTTTGGGGAGTACACCCCATCACACCCTCATCCCACCACCGTCGTAATCTTCCGGGCAAACCTCTGCCACAAGTCAccca                            >  2:18746973/1‑136 (MQ=255)
                                                                                                              aGC‑GCCTGCCAGATCGAGCACCACCCGCACGGCACACAATC‑CTGCAAGCGCTG‑CACCACTGGAGGCGTCCCCCCCATCACACTCTCCTCCCACCACCTTCGTCATCTGCCGGCCCACTCTCTGCCACAGGTCAACCg                            >  2:3211354/1‑137 (MQ=255)
                                                                                                                c‑gcccgccAAATCGAGCACCGCCCACGCGGAACACGATC‑TGGCAGGCGCTG‑CACCATTGGAGGCGTCCGCCCCATCAAATTATCATCAAACCACCGTCGTAACCTGCCGGACAAACCTATGCCAAAAGTCAACCgaa                          >  2:13157264/1‑135 (MQ=255)
                                                                                                                c‑gcccgccAAACCGAACACCACCCACGCGGTACACGACC‑CAGCAAGCGCTA‑TACCATTGGAGGGATACGTCCCGTCACATTATCATCACGCCACCGTTGTAATCTTCTGGTCAACTCTACGCCAAAGGTCGCCCGGc                          >  2:15477267/1‑137 (MQ=255)
                                                                                                                 cggcTGCCCGATCGAGCACCACCCGCGCGGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCCCACTATCCCCACGCCCCTGTCGTAATCTTCCCGCCAACTCTCTGCCCAAAGTCACCCGGc                          >  2:9231121/4‑137 (MQ=255)
                                                                                                                           aTCGAGCACCGCCCGCGCAGCACACGATC‑TGGAAGGCGCTG‑CACTACTTGAGGCGTACGCCCCATCACATTACCACCACGCCACCGTCGTAACCTGCCGGCCAAATCCATGCCAAAAGTCACCCGGCATGCTCCGcc                >  2:507299/1‑137 (MQ=255)
                                                                                                                              gAGCACCGCCCGCGCGGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCNCATTATCATCACGCCACTGTCGTAATCTGCCGGCCAAATCTATGCCACAGGTCAACCGGCATGCTCCGCCTGc             >  1:17390492/1‑137 (MQ=255)
                                                                                                                              gAGCACCGCCCGCGCGGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc             >  2:3427307/1‑137 (MQ=255)
                                                                                                                              gAGCACCGCCCGCGCGGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc             >  1:12513503/1‑137 (MQ=255)
                                                                                                                                    cgcccgcGCGGCACACGATC‑TGGAAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCt       >  1:15043768/1‑137 (MQ=255)
                                                                                                                                       ccgcGCGGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgc    >  2:1882243/1‑137 (MQ=255)
                                                                                                                                        cgcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct   >  2:9190431/1‑137 (MQ=255)
                                                                                                                                        cgcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct   >  1:9702493/1‑137 (MQ=255)
                                                                                                                                        cgcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct   >  1:15426710/1‑137 (MQ=255)
                                                                                                                                        cgcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct   >  1:15426709/1‑137 (MQ=255)
                                                                                                                                        cgcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct   >  2:15118373/1‑137 (MQ=255)
                                                                                                                                        cgcgcgACAAACGATT‑GGGCAGCCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct   <  2:12806973/137‑1 (MQ=255)
                                                                                                                                         gcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGc                                                    <  2:18739198/87‑1 (MQ=255)
                                                                                                                                         gcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:8493917/1‑137 (MQ=255)
                                                                                                                                         gcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:15312862/1‑137 (MQ=255)
                                                                                                                                         gcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:6847558/1‑137 (MQ=255)
                                                                                                                                         gcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:6847519/1‑137 (MQ=255)
                                                                                                                                         gcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:2995073/1‑137 (MQ=255)
                                                                                                                                         gcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:18360330/1‑137 (MQ=255)
                                                                                                                                         gcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:10067431/1‑137 (MQ=255)
                                                                                                                                         gcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:9097127/1‑137 (MQ=255)
                                                                                                                                         gcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:7565640/1‑137 (MQ=255)
                                                                                                                                         gcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:5992642/1‑137 (MQ=255)
                                                                                                                                         gcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:478235/1‑137 (MQ=255)
                                                                                                                                         gcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCCGCTTGTCTtgctg  >  2:15971071/1‑137 (MQ=255)
                                                                                                                                         gcgcgGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCAACCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:18749287/1‑137 (MQ=255)
                                                                                                                                         gcgcgGCACACGATC‑CGGCAGGCGCTG‑CACTCTTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:9947586/1‑137 (MQ=255)
                                                                                                                                         |                                                                                                                                          
CGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATC‑TGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTG  >  AE015451_phaM1/4344176‑4344448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑