| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AE015451_phaM1 | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | trpS CDS (Tryptophan‑‑tRNA ligase) → / → zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451_phaM1 | 1,499,480 | 0 | C | A | 95.2% | 449.9 / NA | 207 | intergenic (+57/‑107) | trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Reads supporting (aligned to +/- strand): ref base C (10/0); new base A (101/96); total (111/96) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.97e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | AE015451_phaM1 | 1,499,481 | 0 | A | C | 97.5% | 480.2 / NA | 207 | intergenic (+58/‑106) | trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Reads supporting (aligned to +/- strand): ref base A (4/0); major base C (107/91); minor base T (0/5); total (111/96) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.24e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 207 total aligned reads displayed.
TTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTG‑T‑T‑T‑CGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATC > AE015451_phaM1/1499352‑1499608 || ttCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCttt < 2:5230731/137‑1 (MQ=255) tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTg < 2:10421838/137‑1 (MQ=255) tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTg < 1:9372552/137‑1 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGcc < 2:6937/137‑1 (MQ=255) cAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCgg < 2:4894192/137‑1 (MQ=255) aCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgtg < 2:1069215/137‑1 (MQ=255) cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTTGCACCACAGCAGGACTGAGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgtg‑t < 2:4264074/137‑1 (MQ=38) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGTCCGCGAAACAGGCGCCGCGGTGCATGGCCCCGGCTTTGCCGGTG‑t‑tcg > 2:10847414/1‑135 (MQ=255) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCGCGAAACAAGAGCCACGGTGCATGGCCCCGGCTTTTGCAGTG‑t‑t‑tt > 1:10837755/1‑136 (MQ=255) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGATGACCACGAAACAAGCACCACAGTGCATGGCACCGACTTTGTCCGTT‑T‑T‑T‑c > 1:1138912/1‑137 (MQ=255) aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCGCGAAACAAGCGCCACGGTGCACGGCACCAGCTTTGCCGGTG‑t‑tcgcg > 1:11774809/1‑133 (MQ=255) aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCACGAAACAGGCGCCGCGGTGTATGGCACCGGCTTTGCCGGTG‑t‑tcgcg > 2:15529424/1‑133 (MQ=38) aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGGGAAACAGGGGCCGCGGTGTATGGCACCGGCTTTTCCGGTG‑t‑t‑tgcg > 1:13578118/1‑134 (MQ=38) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCACGGTGCCCGGCACCGGCTTTGCCGGTG‑T‑TCG‑CAGGCAc‑g‑cc > 2:8178206/1‑137 (MQ=25) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCACGGTGCATGGCACCGGCTTTGTCGGTG‑T‑TCG‑CGGGCAc‑g‑cc > 2:254030/1‑137 (MQ=25) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAGGCACCGCAGTGTATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAc‑g‑cc > 1:12830544/1‑137 (MQ=25) tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGCCCCCGAAACAAGAGCCACAGTGCATGGCCCCCGCTTTTTCTGTG‑T‑TCT‑CGGGAAc‑g‑cccc > 1:1591178/1‑135 (MQ=255) tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAACAAACGCCACGGTGCCCGGCACCCGCTTTTTCTGTGTT‑T‑T‑CGGGCAc‑a‑cccc > 1:7210195/1‑135 (MQ=255) tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAc‑g‑cccg > 2:8600903/1‑135 (MQ=25) gCCATTCAGAGCTTGAAAGAAGCTTTGGCACCACAGCGGGATGGGGGCTGACAAGCGGGGGGAGTGGGCGTTCCCGAGAAAAGGGCGCCGCGGTGCATGGCACCGGCTTTGCTGGTG‑T‑TCG‑CGGGCAC‑TCCCGCTcc < 1:9136802/137‑1 (MQ=12) ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTccc > 2:2146854/1‑137 (MQ=21) ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCGCGGTTCACGGCACCGGCTTTGCCAGTG‑T‑TCG‑CAGGCAC‑GCCCGCTccc > 2:10757387/1‑137 (MQ=18) aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCcac > 2:8386896/1‑137 (MQ=21) aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCATGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCcac > 2:11755414/1‑137 (MQ=21) cAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCAAGAAACAGGAGCCGCGGGGCATGGCACCGGATTTGCCAGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGa > 1:2601955/1‑137 (MQ=21) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCCCCGGGTTTGTCGGTG‑T‑TCG‑CGGGCCC‑GTCCGCTCCCACAGACTGGtt > 2:7634847/1‑137 (MQ=21) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAA‑GCCCGCTCCCACAGACCGATTGc > 2:5207217/1‑137 (MQ=18) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCACGGCACCGGCTTTGCCAGTG‑T‑TCG‑CGGGCAC‑GCCCACTCCCACAGAATGATTGc > 2:16211417/1‑137 (MQ=18) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCAAAACAGGAGCCGCAGTGTATGGAACCGGATTTGCCAGTG‑T‑TCG‑CAGGCAC‑GCCCCCTCCCACAGAATGGTTga > 1:14015697/1‑136 (MQ=16) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAAAAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCACTCCCACAGACTGATTGc > 2:15649237/1‑137 (MQ=21) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGACCGCGAAAAAGGCACCGCGGTGCATGGCACCAGCTTTGGCGGTT‑T‑TCA‑CGGGCCC‑GCCCGATCCCACAGGCTGATTGc > 2:11411147/1‑137 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACCAGCTTTTCCGGTG‑T‑TCT‑CGGGCACAC‑CCACTCCCCCAAACCGATTTCCAtt > 1:8251449/1‑137 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGCCCACGAAACAAGAGCCGCGGTGTATGGCACCAGCCTTTCCTGTGTT‑T‑T‑CGGGCACAC‑CCACTCCCAAAAACTGATTGCCAtt > 1:3842728/1‑137 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGGCCGCGAAACAAGAGCCGCGGTGTATTGCACCAGCTTTGCCGGTG‑T‑T‑TGCGGGACC‑GCCCACTCCCACAGAATGAGTGACAtt > 2:14493285/1‑137 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAAACTGATTGCCAtt > 2:17476067/1‑137 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCACGGTGCACTGCACCAGCTTTTCCGGTG‑T‑TCG‑CGGGCAC‑GCCCCCTCCCACAAAATGATTGCCAtt > 2:8098455/1‑137 (MQ=18) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGAGCCGAGGTGCACGGCAACAGCTTTGCCGGTG‑T‑TCG‑TGGGCAC‑GCCCGCTCCCACAGAATGAGTGCCAtt > 2:14394106/1‑137 (MQ=18) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCAAAAAAAGCGCCGCGGTGCATGGCACCGGCTTTTCCGGTG‑T‑T‑TGCGGGCACAC‑CCGCTCCCACAGAATGATTGCCAtt > 2:12625641/1‑137 (MQ=18) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGTCCGCAAAACAGGAGCCACGGTGCATGGCACCGGCTTTTCCGGTGTT‑T‑T‑CGGGCAC‑GCCCACTCCCACCGACGGATTGCCCtt > 1:17232763/1‑137 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAAGAGCCACAGTGCACGGCACCAGATTTGCCAGTG‑T‑T‑TGCGGGCAC‑GCCCACTCCCACAAAATGATTGCCact > 2:6169870/1‑135 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCACGAAACAAGCACCACGGTGTCTGGCACCAACTTTTCCGGTGTT‑T‑T‑TGGGCACAC‑CCACTCCCCCAAAATGATTGCCAtt > 1:5430246/1‑137 (MQ=255) ccTTGACCCCACAACAGGCCTGAGGCTGCCAAACGGTGGGGAGGGGCGTACCCGCAAAAAGTACCCCGCGGTACGTAACACCGGCTTGGCTGGGG‑TCA‑G‑TGGGCAC‑GCCCGCTCCCACAGACTGATTACCATTAAGc < 2:3107489/137‑1 (MQ=255) ttGGCACCACAGCGGGACTGAGGCTGCAAAAAGGTGGGAGCGGGTGTGCCAGAGAAACGGGCGCTGTGGTGCGGGGCACCGGTTTTGCTGGTG‑T‑T‑CGCGGGAAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 2:10918848/137‑1 (MQ=255) ttGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGATTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGAATGATTGCCATTAAGCgg > 2:1846857/1‑137 (MQ=21) ttGGAACCACAGCAGGACTGAGGTTGCCAACCGGTGGGAGTGGGCGTACCCGCAAAACAGGCGCCGTGGTGCATGGCACTGGTTTTGCCGGTG‑T‑T‑TGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 2:17361721/137‑1 (MQ=21) tgggCACCACAGCAGGACTGAGGCTGCCAAATTGTGGGAGCGGGCGTGCCTGCGAAACAGTCGCCGCGGTGCGTGGCACCGGTTTTGCAGTTG‑T‑TCG‑TGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 2:3475749/135‑1 (MQ=18) tGGCACCACAGCGGGATTGAGGTTGCCAAATGGTGGGAGCGGTTGTTCCCGCGAAACGGGCGCCGTGGGGCAGGGAACTGGCTTTGCTGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCggg < 2:1833063/137‑1 (MQ=16) gaaccacAGAAGGATGGGGGCTACAAAATGGGGGGAGCGGGTGTTCCCGAGAAACAGGCGCCGTGGGGCGTGGCACTGGTTTTTCCGGTT‑T‑TCG‑CGGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 1:9341229/135‑1 (MQ=255) gCACCACAGCAGTACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACGGGCGCCGTGGTACATGGCACTGGCTTTTCCGGTG‑T‑TCG‑CGGGCAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 2:11886665/137‑1 (MQ=18) gCACCACAGCAGGATTGAGGCTGCAAAATGGTGGGGGCGGGTGTGCCCGCGAAAAGGGCGCCGCGGTGCATGGCACCGGTTTTTCTGTTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 2:8899140/137‑1 (MQ=16) gCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGAGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 2:16713815/137‑1 (MQ=21) gCACCACAGAAGGGTGGGTGCGGCAAAATGGGGGGAGTGGGAGTTCCCGAGAAACAGGCGCCGGGGGGTGTGTCACTGGTTTTGTTGTTG‑T‑T‑CGTGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 1:2070509/137‑1 (MQ=255) gCACCACAGAAGGATTGAGGCTGCAAACCGGTGGGAGGGGGCGTTCCCGAGAAAAGGGCGCCACGGGGCGGGGCACTGGTTTTGCTGGGG‑T‑T‑TGCGGGCAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 1:12384849/137‑1 (MQ=255) acaccccAGCAGGACTGGGTTTGCCAAACGTGGGGATCGGTTTTCCCTGCGAAACGGGCGCCGTGGTGCGTGCCACCGTTTTTACCGGTG‑T‑TCG‑CGGGCAC‑ACCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 1:6619821/136‑1 (MQ=255) acaccacAGAAGGGTGGAGGTTGCAAAATGGGGGGAGGGGGTGTTCACGAAAAAAGTGCGCCGTGGGGCAGGGCACTGGTTTTGCGGGTG‑T‑TCG‑TGGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 2:5584688/136‑1 (MQ=255) aaacaacaGAAGGATGGGGGCGGCAAAATGGGGGGGGGGGGGGGGCCCGCGAAAAGGGCGCGGTGGTGAGGGGCATTGGTTTTGCGGGTG‑T‑TCG‑TGGACAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 1:3337782/132‑1 (MQ=255) gcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCACGGTGCCTGGCACCGGCTTTGTCGGTG‑T‑TCG‑AAGGCACAC‑CCGCTCCCACAGGATGATTGACATTAAGCGGGACTATCGCTa > 2:1312640/1‑137 (MQ=18) gcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAAGCGCCACGGTGCATGGCACCGGCTTTGTCGGTG‑T‑T‑TACGGGCAC‑GCCCGCTCCCACAGACCGATTGCCATTAAACGGGACTATCGccc > 2:1020820/1‑135 (MQ=18) aCTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCACAAAACAAGAGCCACGGTGTATGGCACCAGATTTTTCGGTG‑T‑TCA‑CGGGCACAC‑CCACCCCCCCAAACTGATTGCCATTAAACGGGCCCATCGCTGCAGTg > 1:4112236/1‑137 (MQ=255) tGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAGGCGCCACAGTGCACGGCACCAGCTTTGCCGGTG‑T‑T‑TGCGGGCACAA‑CCGCTTCCACAGACTGATTGCCACTCAGCAGGCCTACCACTACAATGAc > 2:1346109/1‑137 (MQ=255) ggAGGCTGCAAAATGGTGGGGGGGGGTGTGCCCGAAAAACGGGCGCCGCGGTGCATGGCATTGGTTTTTCTGGTG‑T‑T‑CGAGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc < 2:14789684/136‑1 (MQ=255) gAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCAGTTCACGGCACCGGCTTTGCCGGTT‑T‑TCG‑CGGGCCC‑GCCCGCTCCCACCGACTGATTGCCATTAAGCGGGCCTATCGCCACAGTGAca > 2:9880844/1‑136 (MQ=18) cTGCCAAACGGTGGGAGCGGGCGTTCCCGCGAAACAGACGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑TGGGCAC‑GCCCGCTCCCAAAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGcac > 2:7252958/1‑135 (MQ=21) cTGCCAAACGGTGGGAGCGGGCGTGTCCGCGAAACAGGCGCCGCGGTGCCTGGCCCCGGCTTTGCCGGTG‑T‑T‑TGCGGGGAC‑GCCCGCTTCCACAAACTGATTGCCATTAAGCGGGCCTATTGCTACAGCGACGGccc > 2:6722324/1‑137 (MQ=37) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGAATGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc > 2:17104684/1‑137 (MQ=18) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCACGGCACCGGATTTGCCAGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAAAATGATTGCAATTCAACGGGCCTATCGCCACAATGACGGccc > 2:12508491/1‑137 (MQ=17) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAGGCGCCACGGTGCATGGCACCGGATTTGTCGGTG‑T‑T‑TGCGGGCACAC‑CCACTCCCACAGACTGGTTTCCCTTCAGCGGAACTGTTGCTGCAGTGAAGGgcc > 1:18470448/1‑137 (MQ=9) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCCCAGACTGATTGCCCTTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttt > 2:18772385/1‑137 (MQ=21) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCACGtttt > 2:9333810/1‑137 (MQ=21) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTCAGCGGGCCTCTCGCCACAGTGACGGCCCGtttt > 2:13888989/1‑137 (MQ=18) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCGCGGTGCATGGCCCCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑ACCCGCTCCCCCAGAATGATTGCCATTCAGCGGGCCTATCGCTACCGTGACGGACCGtttt > 1:4223472/1‑137 (MQ=18) aaaCGGTGGGAGCGGGCGTGCCCGCAAAACAGGCGCCGCGGTGCCCGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCACAC‑CCGCTCCCAAAGAATGATTGCCATTAAGTGGGCCTATCGCTACAATGACGGCCCGtttt > 2:2726313/1‑137 (MQ=18) aCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACCGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt > 2:15937897/1‑137 (MQ=18) tttGGGAGCGGGCGTCCCCGCGAAATAGGCGTCGTGGGGCAGGGCACTGGTTTTGCTGGTG‑T‑TCG‑CGGGCAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt < 1:5784409/135‑1 (MQ=255) gAGCGGGCGTGCCCGCGAAACAAGCGTCCCAGTGCCTGGCCCCAGCTTTGTCCGTG‑T‑TCG‑CCGGCACAC‑CCGCTCCCACCAACTGATTGTCATTAAGCGGGCCTCCCTCTCCAGTGGCGGACCGTTTTTTGTTGCCt > 2:400945/1‑137 (MQ=9) gCGGGCGTGCCCACGAAAAAAGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTT‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTCAGCGGGCCTCTCGCTACAGTTGCGGCCCGTTTTTTGTTGCCTCg > 2:18213356/1‑137 (MQ=18) gggTGTGCCTGAGAAACAGGCGCCGCGGTGCGTGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCt < 2:16479936/137‑1 (MQ=25) ccGCGAAAAGGGCGCCGTGGTGCGTGGCATTGTTTTTGCGGGTG‑T‑TCG‑TGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc < 2:15865049/137‑1 (MQ=255) ccGCGAAAAAGGCGCCGGGGTGCGTGGAACCGGTTTTCCTGGTG‑T‑TCG‑TGGGAAC‑ACCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc < 2:1423918/137‑1 (MQ=255) tgcgAAACAGGTTCCGTGGGGCAGGGCACTGTTTTTGTGGGTG‑T‑T‑TGGGGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:12828264/136‑1 (MQ=255) cgcgAAACGGGCGCCGCGGTGCATGGCACCGGTTTTTCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:6310582/137‑1 (MQ=25) cgcgAAACAGGTGCCGCGGTGCATGGCACCGTCTTTACCGTTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:1977617/137‑1 (MQ=37) cgcgAAACAGGCGCCGCGTTGCATGGCACCGGTTTTGCTGGTG‑T‑TCG‑AGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:15171002/137‑1 (MQ=25) cgcgAAACAGGCGCCGCGGTGCATGACACCGGCTTTTCCGTTG‑T‑TCG‑CGGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:15655868/137‑1 (MQ=255) cgcaAAACGGGCGCCGCGGTGCATGCCACCGTTTTTTCCGTTG‑T‑TCT‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:18116023/137‑1 (MQ=255) cgagAAACGGGCTCCGTGGTGCATGGAACTGGTTTTGCTGGTG‑T‑T‑CGGGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:1234948/134‑1 (MQ=25) cgagAAACAGGTGCCGCGGTGAGGGGCACCGGTTTTGTTGGGG‑T‑T‑TGTGGGCAT‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:10226358/134‑1 (MQ=255) cacgAAACAGTCGCCGCGGTGCATGACACCGGCTTTGTCGTTT‑T‑CCG‑CGGGCAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:8593520/135‑1 (MQ=255) agcgAAACAGGCGCCGTGGTGCATGGCATCGGTTTTGCTGGTG‑T‑T‑TGCGGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:12434153/136‑1 (MQ=255) agagAAACGGGCGCTGGGGTGCAGGGCACTGTTTTTGCTGGTG‑T‑TCG‑CGGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:14902472/134‑1 (MQ=255) gcgAAACGGGCGCTGTGGGGCAGGGCACTGGCTTTGCTGGTT‑T‑TCG‑CGGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 1:15761641/137‑1 (MQ=255) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:17732192/137‑1 (MQ=25) cttcgcgGGGCAGGGCACTGGTTTTGCTGGTG‑T‑TCG‑CGGACAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 1:10923403/134‑1 (MQ=255) ctccgcGGTGCATGGCACCGGCTTTTCCGGTG‑T‑T‑CGTGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:6490289/135‑1 (MQ=37) ctccgcGGTACGTGGCACTGGCTCTGCCGGTT‑T‑TCG‑GGGGCACTC‑CCGCTCACACAGACTGAGTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 1:5500727/135‑1 (MQ=255) cgccgtGGTGCGTGACACCGTCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:7882999/137‑1 (MQ=37) cgccgcGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa > 2:10681648/1‑137 (MQ=37) caccgcGGTGCATGGCACCGGCTTGTCCGGGG‑T‑TCG‑TGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:7414084/135‑1 (MQ=25) cgGTGCATGGCACCTGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg < 2:13705471/137‑1 (MQ=37) cgGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg < 2:17891435/137‑1 (MQ=37) || TTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTG‑T‑T‑T‑CGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATC > AE015451_phaM1/1499352‑1499608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |