BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	4,344,312	0	G	A	42.5%	23.9 / 49.7	106	intergenic (+43/‑113)	PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)	–/–
Reads supporting (aligned to +/- strand): ref base G (31/15); new base A (34/0); total (91/15)
Fisher's exact test for biased strand distribution p-value = 8.70e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 169 total aligned reads displayed.

CGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTG  >  AE015451_phaM1/4344176‑4344448

cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgcccg                                                                                                                                               <  1:15534507/137‑1 (MQ=255)

cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgcccg                                                                                                                                               <  1:15534481/137‑1 (MQ=255)

  aCAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcg                                                                                                                                             <  1:12128782/137‑1 (MQ=255)

    aGCTTGAAGGTGCGGTGGGCAGTTGCAGACGTTTGCAGTTGTACCGAAGGGGTTGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTGGCGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                           <  1:4676547/137‑1 (MQ=255)

    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGTGCCGAGAGCGACCTGAGCAAGTCTGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                           <  2:8913025/137‑1 (MQ=255)

    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                           <  1:10652580/137‑1 (MQ=255)

    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                           <  2:13058253/137‑1 (MQ=255)

    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                           <  2:12489340/137‑1 (MQ=255)

    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                           <  1:6901375/137‑1 (MQ=255)

    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                           <  1:3970483/137‑1 (MQ=255)

     gCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCgg                                                                                                                                          <  1:2130905/137‑1 (MQ=255)

     gCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTGGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCgg                                                                                                                                          <  2:12608820/137‑1 (MQ=255)

      cGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTTCCAAAGTGAGCACCACCCCCCCgga                                                                                                                                         >  1:3388945/1‑136 (MQ=255)

      cGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGc                                                                                                                                         <  1:10193797/137‑1 (MQ=255)

      cGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGc                                                                                                                                         <  1:2620467/137‑1 (MQ=255)

      cGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGc                                                                                                                                         <  2:4863933/137‑1 (MQ=255)

      cGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGc                                                                                                                                         <  1:4614154/137‑1 (MQ=255)

          aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGcaaac                                                                                                                                     <  2:16365974/137‑4 (MQ=255)

          aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                                                     <  2:16738077/137‑1 (MQ=255)

          aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                                                     <  2:637200/137‑1 (MQ=255)

           aGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCGATCGAGCACCGCCCCCTCGGTacaca                                                                                                                                    >  2:11299815/1‑136 (MQ=255)

            ggTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGATGCCAGAGCGAGCACCACCCCCACGGCacacaa                                                                                                                                   >  2:173947/1‑135 (MQ=255)

                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCTGCCAGATCGAATACCGCCCCCGTGGCACACGACCT‑‑‑‑GTCa                                                                                                                        >  2:16480769/1‑137 (MQ=255)

                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGTCCAACCGAGAACCACCCACACGGCACACAATccagca                                                                                                                            >  1:7826109/1‑132 (MQ=255)

                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGTCAGACCGAGCACCCCCCACACAGCACAAAAACT‑‑‑‑GGCa                                                                                                                        >  1:6484021/1‑137 (MQ=255)

                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCAGATCTAGCACCACCCGCGCGGCACACAACCT‑‑‑‑GGCa                                                                                                                        >  2:11512498/1‑137 (MQ=255)

                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCGATCGAGCACCACCCACGCGGCACACAAGCC‑‑‑‑GGCa                                                                                                                        >  1:9517996/1‑137 (MQ=255)

                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCACGCGCGGGACACTGTCT‑‑‑‑GGCa                                                                                                                        >  2:15136416/1‑137 (MQ=255)

                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAAACCTAACACCGCCCCCGCAGTACACTATCtttca                                                                                                                            >  2:17125082/1‑133 (MQ=255)

                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGTCCCATCAAGCACCACCCCCGCGGCACACAACCC‑‑‑‑GGCa                                                                                                                        >  1:7637301/1‑137 (MQ=255)

                     ggCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCCGATCGAGCACCACCCACGCGGCACACGAGCT‑‑‑‑GGTAgg                                                                                                                      >  2:303724/1‑137 (MQ=255)

                     ggCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCCAATCTAGCACCACCCCCACGGCACACAACCT‑‑‑‑GGCaag                                                                                                                      >  2:303723/1‑135 (MQ=255)

                       cAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTTAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                           <  2:5484288/118‑1 (MQ=255)

                        aGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGACCGCCCGATCTAAAACCCACCACGCGGCACACAAGCC‑‑‑‑GGCaaggga                                                                                                                   >  1:9021120/1‑132 (MQ=255)

                          tGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGACCGCCCAATTTAGCACCACCCACGCAAAACACAATCT‑‑‑‑TTCCGGCGCtt                                                                                                                 >  2:104442/1‑136 (MQ=255)

                           ggCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCACCCACACGGCACACGATCT‑‑‑‑GGCAggagatgt                                                                                                                >  1:13766072/1‑131 (MQ=255)

                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCCAAGCTAACACCCCCCCCACTGCACACAACCT‑‑‑‑TGCCGGCGATGCAc                                                                                                              >  2:12093884/1‑137 (MQ=255)

                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGATGCCAGATCAAAAACCACCCCCACAGCACACAATAC‑‑‑‑TGCAGGGGATGCaa                                                                                                              >  1:16038858/1‑136 (MQ=255)

                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCAATCGAGCACCGCCCGCGCGGCACACGATCT‑‑‑‑GGCAGGCGATGCAc                                                                                                              >  2:11228095/1‑137 (MQ=255)

                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCAACCGAACACCCCCCACGCGGCACACAACCT‑‑‑‑GGCCGGCGATGCAc                                                                                                              >  1:17790624/1‑137 (MQ=255)

                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGACTGCCCAACCGAACACCCCCCCCACGGCACACAACCC‑‑‑‑TGCAGGAGCttccc                                                                                                              >  1:3826910/1‑133 (MQ=255)

                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGACCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCCGCCAGATCGAGCACCACCCACGCAGCACACGATCC‑‑‑‑GGCAAGGGATGCaa                                                                                                              >  1:11125988/1‑136 (MQ=255)

                              aGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAATCGGCCGGGGCGCATTAACGCTCCTGTACGTTGCAGCGCCTTCCAGGTCTAACACCGCCCGCGCGGCACACGGCCT‑‑‑‑GGCAGGCACTTCACt                                                                                                             >  2:13185573/1‑137 (MQ=255)

                                  gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCAAATCGAGAACCACCCACACGGCACACTATTT‑‑‑‑GTCAGgcactgcactcctg                                                                                                         >  2:15526776/1‑133 (MQ=255)

                                  gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCAATCGAGCACCCCCCACACGGCCCACAACCT‑‑‑‑TGCAGGCGATGCCCCATTg                                                                                                         >  2:14956201/1‑137 (MQ=255)

                                  gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGACTGCCCGACCGAACACCGCCCACACGGAACACAACCT‑‑‑‑GGGAGGGGATGCACTAGTg                                                                                                         >  1:14741521/1‑137 (MQ=255)

                                         tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCGATCGAGGACCACCCGCGCAGCACAAGAGCT‑‑‑‑GGCAAGCAATGCACTATTGGAGGCGt                                                                                                  >  2:7064461/1‑137 (MQ=255)

                                         tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGACCTCCCGATCGAGCACCGCCCGCGCGGCACACAATCT‑‑‑‑GGCAGGAGCTGCACTACTGGaaggat                                                                                                  >  1:11568267/1‑132 (MQ=255)

                                               ccGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGATGCCCGAGCGAGAAACACCCCCACGGCCCACAACCT‑‑‑‑TGCAAACGCTGGACTCTTTGGGGAGTACGccc                                                                                            >  1:17561223/1‑137 (MQ=255)

                                               ccGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACCCGACCT‑‑‑‑GGCAGGCGCTGACCCCCTGGAGGCGGCCGccc                                                                                            >  2:11996971/1‑137 (MQ=255)

                                                 gAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCAGGCGCTGCACTATTGGAGGCGTACGc                                                                                              >  2:8101383/1‑137 (MQ=255)

                                                 gAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGGCAAGCCTAGCCCCGCCCACGCTGCACACGACCT‑‑‑‑GGCAAGATCCGCACTCTTGGAGGACTACGcccca                                                                                          >  2:16109668/1‑136 (MQ=255)

                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCTGCCCGATCGAGCACCGCCCGTGCGGCACACGAGCT‑‑‑‑GGCAAGGGATGCACCATTTGAGGAGTCCGCCCCGT‑cac                                                                                     >  2:16333994/1‑137 (MQ=255)

                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCTGCCCAATCGAACACCACCCACGCGGCACACGACCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCATACGCCCCAT‑ccc                                                                                     >  2:6304402/1‑135 (MQ=255)

                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGACCGCCCAACCTAACACCCCCCCCACAGAACACAACCT‑‑‑‑GGCAAGCGATGAACCACTGGAGGCGTACGCCCCAT‑cac                                                                                     >  2:5775843/1‑137 (MQ=255)

                                                     gggCCGAGAGCGACCCGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCATACCTAGCACCGCCCCCTTGGCACACGATCT‑‑‑‑TGCCAGCGCCTCAGCCCTTGAGGCGTACTCCCAGT‑cac                                                                                     >  1:13920042/1‑137 (MQ=255)

                                                      ggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGATGTCAGATCGAGAACCACCCCCACGGCACACAAGCT‑‑‑‑CGAAAGAAATGCACTATTTGAGGTGGCCGccccaccccc                                                                                     >  1:5631028/1‑131 (MQ=255)

                                                          gagaGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCCAATCGAGCACCGCCCCCGCTGAACACAAGCT‑‑‑‑GGCAGGTGGTGTACCCCTTGAGGAGGACGCCCCAA‑cacaccat                                                                                >  1:6175387/1‑133 (MQ=255)

                                                          gagaGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCGATCTAGCACCACCCACGCGGCACACGATCT‑‑‑‑TGCAGGGGCTGCACTATTTGAGGCGTACACCCCAT‑CACACTat                                                                                >  2:15675777/1‑137 (MQ=255)

                                                               cGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCAGATCGAGCACCGCCCACGCGGCACACGATCT‑‑‑‑GGCAGGTGCTGGACCATTGGAGGAGTACACCCCAT‑CAAACTatcaccc                                                                           >  2:4591465/1‑134 (MQ=255)

                                                                    tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCCGATCGAGCACCGCCCACGCGGAACACAATCT‑‑‑‑GGCAGGCGCCGCACCCTTTGAGGCGTACGCCCCAT‑CACATTATCCCCACGCCa                                                                      >  2:12543831/1‑137 (MQ=255)

                                                                    tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAAATCGAGCACCCCCCACGCGGCACACAATCT‑‑‑‑GGTAGGAGCTGTAATATTTGGGGCGTAAGCCCCAT‑CACATTCTCCCCACGCCa                                                                      >  2:3293549/1‑137 (MQ=255)

                                                                    tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCAGAGTGGGGACCACCCGCAAGGAACAAAAGCT‑‑‑‑TGGAGGCGATGAACTATTTGGGGCGTACGCCCCGT‑TACATTATCCCCACGccc                                                                      >  2:6895076/1‑136 (MQ=255)

                                                                              ggCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCGATCGAGCCCCGCCCGCACAGCACACGATCT‑‑‑‑TGCAGGCGCTGCACCATTGGCGGCGTACGCCCCAT‑CCCACTATCCTCACACCCCCGTCGTGAt                                                            >  2:1263641/1‑137 (MQ=255)

                                                                                   gggCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCTGTACAAGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CCCATTATCATCACGCCAATGTCGTAATCTGcc                                                       >  2:3818817/1‑137 (MQ=255)

                                                                                   gggCGCATTAACGCTCTTGTACGTTGCAGCGACCGCCAGAGCGAGTACCGCCCGCGTGGCACACGATCT‑‑‑‑TGCAGGCGCTGTACTACTTGAGGGGTACACCCCATCCACA‑TATCATCACGCCAACGTCGGCATCTGcc                                                       >  2:2835533/1‑137 (MQ=255)

                                                                                                              aGCGCCTGCCAGATCGAGCACCGCCCGCGTGGCACCCGATCT‑‑‑‑GGCAGGCGCTGGACTCTTTGAGGAGTACGCCCCCT‑TACATTATCATCACGCTACTTTCGTCACCTGTCGGCCGACCCTCTGCCACAGGTCGcccc                            >  2:13730412/1‑136 (MQ=255)

                                                                                                                cggcTGCCAGATCGAGCACCGCCCACGCGGCACACGATCT‑‑‑‑GGCAGGCGCTGCACCACTGGAGGCGTACGCCCCAT‑CACACTACCATCCCGACACTGTCGTAATCTGCCGGCCCAACCTATGCCAAAGGTCACCCGGc                          >  2:4465762/4‑137 (MQ=255)

                                                                                                                cgcCTTCCAGACCTAGCACCACCCCCGCGGCCCACGATCT‑‑‑‑GTTAAGCGCTGCACCCTTGGAGGCGTACGCCCCGT‑CCCATTTTCATCCCACCCCCGTCGTACTCTTCCTGCCCACCCTCTGCCACCAGTCCCCCGGc                          >  2:14707355/1‑137 (MQ=255)

                                                                                                                cgcCTGCCAGATCGAGCACCACCCACGCGGCCCACAACCT‑‑‑‑GGCAGGCGCTGCACCACTGGAGGCGTACGCCCCGT‑CACACTATCCCCACGCCACCTTCGTCATCTTCCCGCCAACCCCCTGTCACAGGTTACCCGGc                          >  2:9938330/1‑137 (MQ=255)

                                                                                                                cgcCTGCCAAATCGAGCACCGCCCGCGCGGCACACGACCT‑‑‑‑TGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CCCATTGTCATCACGCCAATGTCTTAAGCTGTCAGCCAACTCTGTGCCACAGGTCAACCAGc                          >  2:12786390/1‑137 (MQ=255)

                                                                                                                        cgATCGAGCACCGCCCGCGAGGCACACGATCT‑‑‑‑GGCAGGCGCTGCAACCTTGGAGGCGTCCGCCCCGT‑CCCATTATTATTCCACCAACGTCGGGATCTGCCGGCCAACTCTATGCNCCAGGTCACCCGGCATTCTCCg                  >  2:9343591/2‑137 (MQ=255)

                                                                                                                        aGACCGAACACCGCCCACACGGCACACAACCT‑‑‑‑GGCAAGCGATACAATACTGGAGGCGTACGCCCCAC‑CCAACTATCCTCAAGCCACCTTCATCATCTTCCGGCCAACTCTATGCCACAAGGCCCCCAGCACGCTCCg                  >  2:12595388/1‑137 (MQ=255)

                                                                                                                              aGCACCGCCCGCGCGGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCt            >  2:11258978/1‑137 (MQ=255)

                                                                                                                               ccACCGCCCGCGAGGACCACCCTTT‑‑‑‑AGCATCCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCtt           <  1:7088636/136‑1 (MQ=255)

                                                                                                                                   cgcccgcGCGGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCt       >  2:18516793/1‑137 (MQ=255)

                                                                                                                                   cgcccgcGCGGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCt       >  1:12101990/1‑137 (MQ=255)

                                                                                                                                   cgcccgcGCGGCACACGACCT‑‑‑‑GGCAGGCGATGCACTATTGGAGGCGTACGCCCCGT‑ACCATTATCATCCCGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCt       >  1:6249144/1‑137 (MQ=255)

                                                                                                                                       cgcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct   >  1:14933491/1‑137 (MQ=255)

                                                                                                                                       cgcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct   >  1:6000464/1‑137 (MQ=255)

                                                                                                                                       cgcgcgGCACACAATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct   >  2:7452271/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:8760890/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:8385099/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:14661691/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:18167092/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:8131258/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:7157114/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:2605301/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:3811091/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:14361698/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:5633852/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:16673726/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:13484523/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:18960055/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:17978780/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:13812944/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:1668601/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCAGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:1768373/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAGGCACTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACCGTCGTAATCTGCCGGCCAACTCTATGCAACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:8171862/1‑137 (MQ=255)

                                                                                                                                        gcgcgGCACACGATCT‑‑‑‑GGCAAGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CAAATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCCTGCTCCGCCTGCTTGTCTtgctg  >  2:3142286/1‑137 (MQ=255)

CGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCT‑‑‑‑GGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGT‑CACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTG  >  AE015451_phaM1/4344176‑4344448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`