Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,737,7560AG36.1% 295.5 / 86.2 260intergenic (+45/+26)hutF CDS (probable formiminoglutamate deiminase)/PP_5037 CDS (lipocalin family lipoprotein)–/–
Reads supporting (aligned to +/- strand):  ref base A (30/136);  new base G (0/94);  total (30/230)
Fisher's exact test for biased strand distribution p-value = 7.31e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 264 total aligned reads displayed.

TGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGG‑TGTTGCGCCAGCTTTTGGGCT‑GAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAG  >  AE015451_phaM1/5737621‑5737892
                                                                                                                                          |                                                                                                                                        
          ttggtaCGCCAGGGGCGGCATGCCCAGGAAGAGGAAAGCGGGCGGGCGTTTGCCAAGG‑TGTTGCGCCAGCTTTTGGGCT‑AAGCGTACCGGGGTTGCGTTGCATCCCTTCGCGTGCAAGCCC‑GCGAAGGGTTgcaacg                                                                                                                               <  2:10042002/136‑1 (MQ=255)
            tgagcgcCGGGGGCGGTGTACCCAGAAAGAGAAACGCGGGCGGGCGTTTGCCAAGG‑TGTTGCGCCATTTTTTGGGCT‑GAGCGTATCGGTGTTGTGTTGCAGCCCTTCGCGCGCAAACCC‑GCGAAGTGTTgcaacgca                                                                                                                             <  2:16813040/134‑1 (MQ=255)
                    aGGGGCGGCATTCCCAGGAAGGTGAAAGCGGGCGGGTGTTTTCCAAGG‑TTTTGCGCAAACTTTTGGGCT‑GAGCGTACCGGGGTGGCGTTGCAGCCCTTCGCGTGCAAGCCT‑GTGAAGGGTTGCAACGCAACCCCaaa                                                                                                                     <  2:16527797/137‑1 (MQ=255)
                         ggacccgCCAAGAAAGAGAAAAGCGGGCGGGCGTTTGCCAAGG‑TGTCGCACCAACTTTGGGGCT‑GGGTGTACTGGGGTTTCTGTGCATCCCCTTGCTGGCAATCCC‑GCAAAGGGTTACACCGCAACCCCAAAAAACt                                                                                                                <  2:490556/131‑1 (MQ=255)
                           gcatgcatAGGGAGATGAAAGCGGGGGGGCGTTGGCCGAGG‑TTTTGCGCAAGCTTTGGGGCG‑GAGCGTACCGGGTTGGCTTTGCAGCCCTTCGCGGGCAAGCCC‑GCGAGGGGTGGAAACGCAACCCCAAAAAACTCa                                                                                                              <  2:2997671/137‑1 (MQ=255)
                                            aaaGCGTGTGGGCGTTTACGAGGG‑TTTTGCG‑CAGCCTTTGGGGTGGAGCGTACGGGGGTTGTGTGGAACCCTTTAGTGGGAAAGCCC‑GCGAGGGGTGAAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTAttt                                                                                             <  1:2619134/137‑1 (MQ=255)
                                            aaaGCGGGTGGGCGTTTGCCAAGG‑TTTTGGGCC‑GTTTTTGGGGTGGAGTGTACTGGGGTTGTTTTGCATCCTTTTGCTTGCAAGCCT‑GAGAGGGGTGACACCGCAACCCCAAAAAACTCAGTGCATTTTTACTAttt                                                                                             <  1:14582294/137‑1 (MQ=255)
                                                     ggttgTTTGCCAAGT‑TGTGGCGCTAGCTTTTGGGCG‑GAGCGTATAGGGGTTGCGTTGAGGCTTTTCGCGTGAAAGCCC‑TAGAGGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCgg                                                                                    <  1:11989337/133‑1 (MQ=255)
                                                     gggtgTTTGCCAAGG‑TGTTGCTCCAGTTTTGGGGCT‑GATCGTACCGGGTTTGCTTTGCATCCCTTTGCGTGCAACCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCgg                                                                                    <  1:17343527/137‑1 (MQ=255)
                                                      gggtgttGACAAGGTTGTTGCGCCAGTTTTGGGGCG‑GGGCGTACGGGGTTTGTTTTGCATCCTTTTGTGTACAATCCC‑GCGAGGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCgg                                                                                    <  1:5179134/132‑1 (MQ=255)
                                                        cgTTTGCCAAGG‑TGTTGCGCAAGCTTTTGGGCT‑GAGTGTACTGGGGTTGTGTTGCAGCCTTTCGTGGGCAAGCCC‑GCGAGGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcg                                                                                 <  2:10765228/137‑1 (MQ=255)
                                                         ttttGCCAAGG‑TTTTGCACCAGTTTTTGGGCG‑GAGTGGACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcgc                                                                                <  2:7759742/136‑1 (MQ=255)
                                                         gTTTGCCAAGT‑TGTTTCCCCAGCTTTTGGGCT‑GAGTGCACTGGGGTTGCGTTGCAACCCTTCGCGTGCAATCCC‑GTGAGGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcgc                                                                                <  2:7614725/137‑1 (MQ=255)
                                                             gCCAAGG‑TGTTGCGCCAGTTTTTGGGTT‑GGTCGTACTGGGGTTTCGTTGCATCCCTTCGTTTGCAAGCCC‑GCGAAGGGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcgcccgc                                                                            <  2:9770748/137‑1 (MQ=255)
                                                               aaaGG‑TGTTGCCCCATTTTTTGGGCT‑GATTGTACTGGGGTTGCGTTGCAGCCTTTCGCGTGCAAGCCC‑GCGAGGGGTGGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCa                                                                          <  2:8364094/136‑1 (MQ=255)
                                                                        ttctccAGTTTTTGGTCG‑GATTGTATGGGGGTTGTGTTGCATCCCTTCGTGGACAACCCA‑GCGAGGGGTGAAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  1:8243332/133‑1 (MQ=255)
                                                                        tGCTCCAGTTTTGGGGCG‑GAGTGTACCGGGTTGGTGTTTCATCCCTTCTCGTGCAAGCCC‑GCGAGGGGTTAAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  1:18154107/137‑1 (MQ=255)
                                                                        tGCTCCAGCTTTTGGGCT‑GAGCATACCGGGGTTGCTTTGCATCCCTTCACGTGCAAGCCC‑GGGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  2:11148636/137‑1 (MQ=255)
                                                                        tGCGCCGTCTTTTGGTTT‑GAGCGTACTGGGGTTGTTTTGCGGCCCTTCGCTTGAAAGCCC‑GCAAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  1:16505554/137‑1 (MQ=255)
                                                                        tGCGCCAGCTTTTGGGCT‑GAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGGGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  2:18387289/137‑1 (MQ=255)
                                                                        tGCACCATCTTTGGGGCT‑GAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCC‑GCGAAGGGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  2:8247558/137‑1 (MQ=255)
                                                                        tGCACCAGCTTTGGGGCT‑GAGCGTACCGGGGTTGTGTTGCAGCCCTTTGCGTGCAAGCCC‑GTGAGGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  2:11317564/137‑1 (MQ=255)
                                                                         tgcgcAGTTTTTGGGGTGGGGCGCACGGGGGTGGTGTTGCATCCCTTTGTGGGAAGTCCG‑GGGGGGGGTGGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  1:13444907/133‑1 (MQ=255)
                                                                         tcgcCAACTTTTGGGCT‑AAGCTTCCCGGGGTTACTTTTCAGCACTTTGTGGACAAACCC‑AAGAAGGGGGACAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCg                                                                 <  2:8848380/136‑1 (MQ=255)
                                                                         gtgcCAGCTTTGGGGTG‑GAGGGTATGGGGGTGGTGTGGCAGCCTTTTGAGGAAAAGCCC‑GGGAAGGGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCg                                                                 <  2:12774490/135‑1 (MQ=255)
                                                                         gcgcCATCTTTTGGGCT‑GAGTGTACCGGGTTGGCGTTGCAGCCTTTCGCTTGCAAGCCC‑GTGAGGGGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCg                                                                 <  2:17732861/137‑1 (MQ=255)
                                                                         gcgcCAGCTTTTGGGTT‑GGGCGTGTTGGGGTGGCGTTGCAGCCTTTTGCGGGCAAGCCC‑GCGAAGGTTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCg                                                                 <  2:18518467/137‑1 (MQ=255)
                                                                         gcgcCAGCTTTTGGGCT‑GAGCGCACCGGGGCTGCGTTTCAGCCCTTCGCGTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCg                                                                 <  2:18930941/137‑1 (MQ=255)
                                                                          ctcaaGCTTTTGGGCT‑GAGCGTACTGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCgg                                                                <  2:7637252/133‑1 (MQ=255)
                                                                          cgcCGGTTTTGGGGCG‑GGGTGTACGGGGGTTGTTTGACACCCTTTCGTG‑GGAAGCCCAGAGAGGGGTGGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCgg                                                                <  1:7574714/137‑1 (MQ=255)
                                                                          cgcCAGTTTTTGGGCT‑GAGTTTACTGGGGTTGCTTTGCAGCCTTTCCCGTGCAGGCCC‑GCGAGGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCgg                                                                <  2:9471010/137‑1 (MQ=255)
                                                                          cgcCAGCTTTTGGGCT‑GAGTGTACCGGGGGTGCGTTGCAGCCCTTCCCGGCCAATCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCgg                                                                <  2:2715563/137‑1 (MQ=255)
                                                                          cgcCAGCTTTTGGGCT‑AAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCgg                                                                <  2:14183692/137‑1 (MQ=255)
                                                                          cgcCAGCTTTGGGTTG‑GGGCGTACTGGGTTTGCGTTGCATCCCTTCGTGGGCAAGCCT‑GCGAAGGTTTGCACCGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCgg                                                                <  1:11136399/137‑1 (MQ=255)
                                                                           tccAGCTTGTGGGTG‑GAGTGTATTGGGGTGGTGTTTCAGCCTTTGGTGTGCAAGCCC‑GCGAAGGGTTGCAACACAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGc                                                               <  1:18559913/136‑1 (MQ=255)
                                                                           gcCAGTTTTGGGGTT‑GAGTGTACTGGGGTTGTGTTGCAGCCTTTCGTGTGAAAGCCC‑GCGAGGGGTGGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGc                                                               <  2:6707046/137‑1 (MQ=255)
                                                                                 ttttGGGCT‑GAGCGTACTGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCC‑GCGAGGGGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGgtgt                                                         <  2:8655821/137‑1 (MQ=255)
                                                                                  tttGGGCT‑GAGCGTACCGGGGTTTCGTTGCCGCCCTTCGCGTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAAATCAGTGCATTTTTACTATTTGCTTATCGGACGCCCGCCAGATCCAGCGGCTGGTGTc                                                        >  2:729731/1‑137 (MQ=255)
                                                                                   ttGGGCT‑GAGCGTACTGGGGTTGCGTGTCAACCCTTCGTGTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCa                                                       <  2:4077894/137‑1 (MQ=255)
                                                                                              tggacgggggTTGTGTTGCATCCCTTCGCGTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:10142184/131‑1 (MQ=255)
                                                                                              tgTACTGGGGTTGTGTTGCAGCCTTTCGGGGAAAAGCCC‑GAGAGGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:6908092/136‑1 (MQ=255)
                                                                                              tgTACCGGGGTTGCGTTGCAGCCCTTTGTGTGCAAGCCC‑GCGAGGGGTGACAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:147615/136‑1 (MQ=255)
                                                                                              ggtatgggggTTGCGTGGCGTCCTTTCGCGGGAAAACCC‑GAGGGGGGTGTAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  1:1036299/131‑1 (MQ=255)
                                                                                              gggacgggggTTGTGTTGCAGCCTTTCGCGTGAAAACCT‑GTGAGGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:1297090/131‑1 (MQ=255)
                                                                                              cgtatggggtTTGTGTTGCGTCCCTTCGTGTGCAATCCT‑GCGAGGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  1:3812748/131‑1 (MQ=255)
                                                                                              cggactggggTTGCGTTGCATCCCTTCGTGTAAAAGCCC‑TCGAAGGGTGGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  1:9175050/131‑1 (MQ=255)
                                                                                              cGTACTGGGGTTGTGTTGCATCCTTTCGCGTGCAAGCCC‑GCGAGGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:15112311/137‑1 (MQ=255)
                                                                                              cGTACCGGGGTTGCTTTGCACCCCTTCGCGTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:13930727/137‑1 (MQ=255)
                                                                                              cGTACCGGGGTTGCGTTGCAGCCCTTCGTTTGCAGGCCC‑GCGAAGGGTTGCAACGCAACCTCAAAAAACTCAGTGCATTTTTCCTATTTTCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:4752476/137‑1 (MQ=255)
                                                                                              cGTACCGGGGTTGCGTTGCAGCCCTTCGTGTGAAAGCCC‑GCGAGGGGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:2433193/137‑1 (MQ=255)
                                                                                              cGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:10418579/137‑1 (MQ=255)
                                                                                                 atcGGGGTTGTGTTGCATCCTTTTGTGGAAAAACCC‑GAGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGcc                                          <  1:4860331/135‑1 (MQ=255)
                                                                                                 aCCGGGGTTGCGTTGCAGCCTTTCGCGTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGcc                                          <  2:11242592/137‑1 (MQ=255)
                                                                                                   tggggTTGTGTGGCAGCCCTTCGCGTGCAAGCCC‑GCGAAGGGTGGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                                        <  2:3358264/136‑1 (MQ=255)
                                                                                                   cGGGGTTGCGTTGGAGCCCTTCGCGTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                                        <  1:14449454/137‑1 (MQ=255)
                                                                                                      ggttttgttgcATCCCTCCGCGTGCAAGCCC‑GAGAAGGGTGGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGcc                                     <  1:6627840/131‑1 (MQ=255)
                                                                                                         tgcgttgcATCCCTTCGCGTGCAATCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTg                                  <  2:12281865/137‑1 (MQ=255)
                                                                                                           tgttgcAGCCTTTTGCGTGCAAGCCC‑GCGAGGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGa                                <  1:3225349/136‑1 (MQ=255)
                                                                                                           tgtggcATCCCTTCGCGTGCAAGCCC‑GTGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGa                                <  2:3808044/133‑1 (MQ=255)
                                                                                                            gttgcAGCCCTTCGTGTGCAAGCCC‑GCGAAGGTTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAc                               <  2:14456396/137‑1 (MQ=255)
                                                                                                            gttgcAGCCCTTCGCGTGCAAGCCC‑GCGAAGNGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAc                               <  2:14456416/137‑1 (MQ=255)
                                                                                                               gcATCCCTTCGCGTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagc                            <  2:13529087/137‑1 (MQ=255)
                                                                                                                     tttCGTGGGCAAGCCC‑GCGAGGGGTGGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                      <  1:4257148/136‑1 (MQ=255)
                                                                                                                     cTTCGCGTGCAATCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                      <  1:1953255/137‑1 (MQ=255)
                                                                                                                     cTTCGCGTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                      <  1:15651427/137‑1 (MQ=255)
                                                                                                                     cTTCGCGTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTAGTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                      <  2:1595739/137‑1 (MQ=255)
                                                                                                                     cTTCGCGGGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                      <  2:14887073/137‑1 (MQ=255)
                                                                                                                         gcgTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc                  >  1:790387/1‑137 (MQ=255)
                                                                                                                           gggCAAGCCC‑ACGAAGGGTGGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                <  2:1340228/135‑1 (MQ=255)
                                                                                                                           gTGCAATCCT‑GCGAGGGGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                <  1:4691127/137‑1 (MQ=255)
                                                                                                                           gTGCAATCCC‑GCGAAGGTTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                <  2:18043313/137‑1 (MQ=255)
                                                                                                                           gTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACgt                >  1:5549073/1‑136 (MQ=255)
                                                                                                                           gTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  2:1216791/1‑137 (MQ=255)
                                                                                                                           gTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  2:990397/1‑137 (MQ=255)
                                                                                                                           gTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  2:6583248/1‑137 (MQ=255)
                                                                                                                           gTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  1:12460521/1‑137 (MQ=255)
                                                                                                                           gTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                <  2:6077234/137‑1 (MQ=255)
                                                                                                                                aGCCC‑GCGAGGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCa           <  2:3585880/137‑1 (MQ=255)
                                                                                                                                aGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCa           >  2:1168736/1‑137 (MQ=255)
                                                                                                                                 gCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg          >  2:8970589/1‑137 (MQ=255)
                                                                                                                                 gCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAAATCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg          >  2:18802731/1‑137 (MQ=255)
                                                                                                                                   cc‑GGGAGGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:964296/137‑1 (MQ=255)
                                                                                                                                   cc‑GCGGGGGTTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  1:9759382/137‑1 (MQ=255)
                                                                                                                                   cc‑GCGAGGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAgtc        <  2:11048946/137‑3 (MQ=255)
                                                                                                                                   cc‑GCGAGGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:1055872/137‑1 (MQ=255)
                                                                                                                                   cc‑GCGAGGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  1:17149125/137‑1 (MQ=255)
                                                                                                                                   cc‑GCGAGGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  1:17559725/137‑1 (MQ=255)
                                                                                                                                   cc‑GCGAAGGTTTGCAACGCAACCCCAAAAAACTCATTGCATTTTTACTGTTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:15333814/137‑1 (MQ=255)
                                                                                                                                   cc‑GCGAAGGGTTTCAATGCAACCCCAAAAAACTCAGTGCATTCTTACCATCTGCTGATCGGACGCCCGCCAGATCAGGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:5427128/137‑1 (MQ=255)
                                                                                                                                   cc‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:7570950/137‑1 (MQ=255)
                                                                                                                                   cc‑GCGAAGGGTTGCAACACAACCCCAAAAAACTCAGTGCATTTTTACCATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:17841919/137‑1 (MQ=255)
                                                                                                                                   cc‑GCGAAGGGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:13113068/137‑1 (MQ=255)
                                                                                                                                   cc‑GCGAAGGGTGGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:14241490/137‑1 (MQ=255)
                                                                                                                                    ctgcgAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:12495949/135‑1 (MQ=255)
                                                                                                                                    ctgcgAAGGGTGGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  1:18011188/135‑1 (MQ=255)
                                                                                                                                    c‑gcgAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGcc                                     >  1:16263904/1‑107 (MQ=255)
                                                                                                                                    c‑gcgAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGcc                                     <  2:16263904/107‑1 (MQ=255)
                                                                                                                                      gcgAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCg      <  2:1698083/137‑1 (MQ=255)
                                                                                                                                      gcgAAGGGTGGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCg      <  2:7364672/137‑1 (MQ=255)
                                                                                                                                          aGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAg  >  2:18341374/1‑137 (MQ=255)
                                                                                                                                          |                                                                                                                                        
TGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGG‑TGTTGCGCCAGCTTTTGGGCT‑GAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCC‑GCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAG  >  AE015451_phaM1/5737621‑5737892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑