Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM1195,6340CT36.3% 1656.5 / inf 1289T380T (ACC→ACT) PP_0168 CDS (putative surface adhesion protein)
Reads supporting (aligned to +/- strand):  ref base C (271/547);  new base T (178/289);  total (449/840)
Fisher's exact test for biased strand distribution p-value = 7.78e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 1400 total aligned reads displayed.

CCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGG  >  AE015451_phaM1/195499‑195768
                                                                                                                                       |                                                                                                                                       
 ccGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGAcaccaccac                                                                                                                                       <  2:8495319/137‑1 (MQ=255)
 ccGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGAcaccaccac                                                                                                                                       >  2:5478423/1‑137 (MQ=255)
 ccGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGCAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGAcaccaccac                                                                                                                                       >  1:12986513/1‑137 (MQ=255)
   ggCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACcg                                                                                                                                     >  1:4482109/1‑137 (MQ=255)
     cgaaTGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACcgc‑g                                                                                                                                  >  1:9754080/3‑137 (MQ=1)
       aaTGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GAc                                                                                                                                <  1:12711290/137‑1 (MQ=40)
       aaTGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GAc                                                                                                                                <  2:2263524/137‑1 (MQ=16)
              tCTACAACAACGGTTTGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACg                                                                                                                         <  2:10745544/137‑1 (MQ=255)
                 acaacaACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCg                                                                                                                      <  1:16704529/137‑1 (MQ=40)
                        cGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAGCTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGt                                                                                                               <  1:6532777/137‑1 (MQ=255)
                        cGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGt                                                                                                               >  2:6853098/1‑137 (MQ=16)
                            tCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGt                                                                                                           <  1:17315328/137‑1 (MQ=16)
                             cGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTc                                                                                                          >  2:10280268/1‑137 (MQ=40)
                             cGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTc                                                                                                          >  2:17233156/1‑137 (MQ=16)
                              gACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCa                                                                                                         >  2:2876598/1‑137 (MQ=40)
                                ccGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCAcc                                                                                                       >  1:11521928/1‑137 (MQ=16)
                                  gTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGa                                                                                                     >  2:3729604/1‑137 (MQ=255)
                                      gCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACAGCGAGCCCGTCGGTCACCGAAggc                                                                                                 >  2:15537206/1‑137 (MQ=255)
                                         tCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                                                                              <  2:6526996/137‑1 (MQ=37)
                                         tCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                                                                              <  1:7102310/137‑1 (MQ=39)
                                         tCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                                                                              <  2:13914307/137‑1 (MQ=40)
                                         tCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                                                                              <  2:8242450/137‑1 (MQ=39)
                                         tCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAggcagc                                                                                              <  2:14710467/137‑4 (MQ=255)
                                         tCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAAGCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                                                                              <  2:18281242/137‑1 (MQ=255)
                                         tCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                                                                              <  1:14200851/137‑1 (MQ=12)
                                         tCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                                                                              <  2:13592912/137‑1 (MQ=16)
                                         tCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                                                                              <  1:3859726/137‑1 (MQ=16)
                                           aCCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt                                                                                            <  2:4482109/137‑1 (MQ=40)
                                               tCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATc                                                                                        <  2:16051041/137‑1 (MQ=37)
                                                 gagaACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc                                                                                      <  2:3406434/137‑1 (MQ=40)
                                                 gagaACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACAGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc                                                                                      <  1:5478423/137‑1 (MQ=255)
                                                 gagaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc                                                                                      <  2:4834852/137‑1 (MQ=16)
                                                     aCGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTac                                                                                  >  1:3211027/1‑137 (MQ=37)
                                                      cGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTaca                                                                                 <  1:9983407/137‑1 (MQ=255)
                                                      cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTaca                                                                                 <  1:18877739/137‑1 (MQ=16)
                                                         cacaggcggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCg                                                                              >  1:13531217/5‑137 (MQ=16)
                                                           ccGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTg                                                                            <  2:12031571/137‑1 (MQ=255)
                                                           ccGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTg                                                                            <  2:12485838/137‑1 (MQ=255)
                                                           ccGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTg                                                                            <  1:11258098/137‑1 (MQ=255)
                                                           ccGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTg                                                                            <  2:1922807/137‑1 (MQ=255)
                                                           ccGGCGGTAAGTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTg                                                                            <  2:4991491/137‑1 (MQ=255)
                                                           caggcggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTg                                                                            <  1:12346460/135‑1 (MQ=17)
                                                            cggcggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATc                                                                                        >  1:1631558/1‑124 (MQ=40)
                                                              gcggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                                                                              >  1:1469033/1‑116 (MQ=18)
                                                                ggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCt                                                                       <  2:6166812/137‑1 (MQ=18)
                                                                ggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCt                                                                       <  1:4746692/137‑1 (MQ=17)
                                                                ggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCt                                                                       <  2:17693252/137‑1 (MQ=40)
                                                                ggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCt                                                                       <  1:17635512/137‑1 (MQ=40)
                                                                ggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCt                                                                       <  2:17951626/137‑1 (MQ=39)
                                                                ggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCt                                                                       <  1:9117333/137‑1 (MQ=38)
                                                                ggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCt                                                                       <  1:11933040/137‑1 (MQ=38)
                                                                   aaTTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTg                                                                            >  2:7895561/1‑129 (MQ=18)
                                                                    aTTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGc                                                                   >  2:5720248/1‑137 (MQ=16)
                                                                          aGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCt                                                             <  2:11351969/137‑1 (MQ=255)
                                                                          aGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCt                                                             >  1:7839890/1‑137 (MQ=255)
                                                                           gCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTg                                                            <  2:5958997/137‑1 (MQ=12)
                                                                           gCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTg                                                            >  1:1027718/1‑137 (MQ=255)
                                                                            cAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGc                                                           >  1:1074245/1‑137 (MQ=37)
                                                                                    cccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGc                                                   <  1:8144192/137‑1 (MQ=37)
                                                                                     ctgactCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGTGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCGGACGcc                                                  <  1:9353707/132‑1 (MQ=11)
                                                                                     ctgAAGCCGACCCCGGCTCAGACCACGATTAACGATTCAGTCGACACCACCACCGC‑GACCCTGACGGCGGGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGcc                                                  <  1:8323552/135‑1 (MQ=11)
                                                                                     ccGAATCCGACCCCTGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGTGTGATCATTTACATCGGGACCCTGAGCAACCCTGCCCAGACGcc                                                  <  1:3651847/137‑1 (MQ=11)
                                                                                     ccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGcc                                                  <  2:743332/137‑1 (MQ=37)
                                                                                     ccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGcc                                                  <  1:2507285/137‑1 (MQ=255)
                                                                                          tCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGtga                                             >  1:2817776/1‑135 (MQ=9)
                                                                                          tCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTaa                                             >  1:18498710/1‑137 (MQ=39)
                                                                                          cccGACCCCTGCTCAGACCACGATCAACGACTCGGTAGACAACACTACCGC‑GACCCGAAAGGCGAGCCAGTCGGGCACCGAAGGCGGCGGGAGCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTaa                                             <  2:1168708/136‑1 (MQ=11)
                                                                                            cGACCCCGGCTCAAACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTaaac                                           >  1:15163727/1‑135 (MQ=255)
                                                                                               ccccGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTg                                        <  2:1675398/137‑1 (MQ=255)
                                                                                               ccccGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTg                                        <  1:10013656/137‑1 (MQ=255)
                                                                                                cccGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGa                                       <  1:13737227/137‑1 (MQ=40)
                                                                                                 ccGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGaa                                      >  2:3129700/1‑137 (MQ=37)
                                                                                                 ccGGCTCAGACCACGATCAACGACTCGGTCGACACC‑CCTCCTCTTACCCCCACCTCCAACCCCCCGATCCACGACACCCGCTTGATCACTTACACCGTGACCCTGAGCAACCCTGCAAAGAAGAAGGTAACAGTGaa                                      >  1:5143667/1‑137 (MQ=2)
                                                                                                           ccACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAAc                            <  1:13316539/137‑1 (MQ=255)
                                                                                                           ccACGATCAACGACGCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAAc                            <  2:2582779/137‑1 (MQ=255)
                                                                                                            cACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACg                           <  1:5793699/137‑1 (MQ=255)
                                                                                                            cACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACg                           <  1:8543935/137‑1 (MQ=255)
                                                                                                            cACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACg                           <  2:12850027/137‑1 (MQ=255)
                                                                                                            cACGATCAACGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACg                           >  2:13230293/1‑137 (MQ=255)
                                                                                                                 tCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCaa                      >  1:15866130/1‑137 (MQ=255)
                                                                                                                   aaCGACTCGGTCGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAAc                    <  2:9378573/137‑1 (MQ=255)
                                                                                                                   aaCGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAAc                    <  1:2097007/137‑1 (MQ=255)
                                                                                                                       aCTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCAAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGAtt                >  2:16960621/1‑137 (MQ=255)
                                                                                                                             tCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGTGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGtt          <  2:11090033/137‑1 (MQ=255)
                                                                                                                              cGACACTACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTg         <  1:7303470/137‑1 (MQ=255)
                                                                                                                              cGACACCACTACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTg         >  2:1417473/1‑137 (MQ=255)
                                                                                                                              cGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTg         >  1:60994/1‑137 (MQ=25)
                                                                                                                              cGACACCACAACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTg         <  2:16380459/137‑1 (MQ=255)
                                                                                                                               gACACCACTACCGC‑GATCCGGACGGCGAGCCTGTCGGTCACCGAAGGCGGTGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGa        <  2:9064216/137‑1 (MQ=14)
                                                                                                                               gACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGa        <  2:18626651/137‑1 (MQ=255)
                                                                                                                               gACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGa        <  1:41450/137‑1 (MQ=255)
                                                                                                                                    caccaccGC‑GACCCTGACTGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg   <  1:18182407/137‑1 (MQ=255)
                                                                                                                                    caccaccGC‑GACCCTGACGGCGGGCCCGTTGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg   <  1:10991805/137‑1 (MQ=255)
                                                                                                                                    caccaccGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAATGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg   <  2:4085769/137‑1 (MQ=255)
                                                                                                                                    caccaccGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCGCTGACGCCGTGACTCGGAGCGGACCTGCCCAGACGCTGTGGACCGTGACCCTGTCCAACGGCCAGACGATTACCGTTGAAGccg   <  1:18697282/137‑3 (MQ=1)
                                                                                                                                    caccaccGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGccg   <  2:8777124/137‑3 (MQ=255)
                                                                                                                                    caccaccGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGccg   <  1:16960621/137‑3 (MQ=21)
                                                                                                                                    caccaccGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg   <  1:15525228/137‑1 (MQ=255)
                                                                                                                                    caccaccGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg   <  2:15017913/137‑1 (MQ=255)
                                                                                                                                    caccaccGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg   <  1:8791221/137‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGC‑GACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGG  >  AE015451_phaM1/195499‑195768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑