| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AE015451_phaM1 | 4,740,816 | T→G | intergenic (+53/+700) | gltA CDS (citrate synthase) → / ← yeiW CDS (putative oxidoreductase) | –/– |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451_phaM1 | 4,740,816 | 0 | T | G | 95.6% | 1213.9 / ‑4.5 | 341 | intergenic (+53/+700) | gltA CDS (citrate synthase)/yeiW CDS (putative oxidoreductase) | –/– |
| Reads supporting (aligned to +/- strand): ref base T (0/15); new base G (196/130); total (196/145) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.74e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 341 total aligned reads displayed.
AGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTT > AE015451_phaM1/4740690‑4740943 | cGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctc < 2:14318180/137‑1 (MQ=255) cGGACGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTTCT‑GTGCCGGCctc < 1:17324425/137‑1 (MQ=255) ggCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct > 2:253274/1‑137 (MQ=255) ggCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct > 1:11927403/1‑137 (MQ=255) gTCCGCGCCAGCTGTACACCGGCGAGCCGAAGCGCGAAATCGTTGCCCTGAAAGAACGCAAGTAAGCTGCAGTAGCCGAACGCAAGAGGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCCCTTcgc > 1:1388625/1‑137 (MQ=255) gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTTCCGGCCCCTTctc > 1:10356196/1‑135 (MQ=255) gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc < 2:7682750/137‑1 (MQ=255) gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc > 1:13649870/1‑137 (MQ=255) gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc > 1:2993558/1‑137 (MQ=255) gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc > 1:18942770/1‑137 (MQ=255) ccGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCgg < 1:11027053/137‑1 (MQ=255) ccGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCgg < 2:1727792/137‑1 (MQ=255) ccGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCgg < 1:6240571/137‑1 (MQ=255) ccGCGCCAGCTGTACACCGGCGAGCAGAAGCGCAACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCgg < 2:10451219/137‑1 (MQ=255) acacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa > 1:4693737/1‑137 (MQ=255) cacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAGGGAAGCTACAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAag < 2:7985197/137‑1 (MQ=255) acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga < 2:429181/137‑1 (MQ=255) acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga > 2:16652107/1‑137 (MQ=255) cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCAGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAgg > 2:1138392/1‑137 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAATGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTTCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGc < 2:6245100/137‑1 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCTGCCCTGCCCGCGAAAAGGCCgga > 1:10037459/1‑136 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGc > 2:13155685/1‑137 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGc > 2:282796/1‑137 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAAAGGCCgga > 2:17062227/1‑136 (MQ=38) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCACGAAAAGGACGGc > 1:1662319/1‑137 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCAGCACTGACCGCGAAGAGGCCAGc > 1:13440232/1‑137 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCACAGCCCTGCCCGCGAAGAGGCCAGc > 2:15585106/1‑137 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCCCTTCGCGGCCTTGCCCGCGAAGAGGccaga > 1:7711722/1‑134 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAG‑CATTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTTCCCACAAAGAGGccaga > 1:5173489/1‑134 (MQ=255) cAGAAGCGCGACGTCGTTGCCCTGGAAGACTGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTTCCCCCGGGCAGCC‑TTTTTTATTTGTCCTGTCCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGcctacac < 2:6812328/137‑6 (MQ=255) aaGAAGCGCGACGTTGTTGCCCTGAAAGACCGCAGGTAAGCTGCGGTAGCCGAACGAAAGAAGGCTGCCTTTGGGCAG‑CTTTTTTTGTTTGTCTTGTGCCGGCCTCTTCGCGGCTTTGCCCGCGAAGAGGCCGGcac < 1:2090894/136‑1 (MQ=255) aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTCGCAGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTCTTTGTCCTGTGCCGGCCTCTTCTCGGCCTTGCCCGCGCAGAGGGCTGcaca > 1:7839174/1‑137 (MQ=255) aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGGGGCCGGcaca > 2:13704645/1‑137 (MQ=255) aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCAGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGcaca > 2:11790309/1‑137 (MQ=255) aGAAGCGCGAACTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGCACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGcaca > 2:4600319/1‑137 (MQ=255) aaGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGGCGGCACAGa > 1:9260654/1‑137 (MQ=38) cgcgACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCAGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGaca‑c > 2:11553699/1‑137 (MQ=39) cgACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCAGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGAca‑cca > 2:15145354/1‑137 (MQ=39) cATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACaa > 2:18625869/1‑137 (MQ=255) aaaGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGttttt < 1:12258892/137‑1 (MQ=255) aGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTCCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTtct < 2:5016061/137‑1 (MQ=255) cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTccgc < 2:3397323/137‑1 (MQ=255) cGCAAGGAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTccgc < 1:2782996/137‑1 (MQ=255) cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTccgccc > 2:8805382/1‑137 (MQ=255) cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAAACTCAGTTTTTCTccgccc > 1:17207700/1‑137 (MQ=39) cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGAAA‑CCACAAAAACTCAGTTTTTCTccgccc > 1:18511214/1‑137 (MQ=38) aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCTGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTccgcccg > 1:12194501/1‑137 (MQ=255) aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGAAA‑CCCCAAAAACTCAATTTTTCTccgcccg > 2:2029664/1‑137 (MQ=38) aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAAAGGCCGGCACAAACA‑CCACAAAAACTCAGTTTTTTTccgccca > 1:7115810/1‑136 (MQ=37) aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCAGCCCTGCCCACGAAGAGGCCGGCCCAAACA‑CCCCCAAAACTCAGTTTTTCTccgcccg > 1:18444058/1‑137 (MQ=255) aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGACTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCCCAAAAACTCAGTTTTTCTccgcccg > 1:17873608/1‑137 (MQ=38) ggaaGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCt < 2:12895165/135‑1 (MQ=255) gTAAGCTGCGGTAGCCGAATGAAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGTCCTCTTCGCGGCCTTGCCTGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCt < 1:11781879/137‑1 (MQ=255) aaGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAG‑CTTTTTTTATTTGTCTTGTGCTGGCCTCTTCGTGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCa < 1:6822743/137‑1 (MQ=255) gCTGCAGTAGCCGAATGCAAGAGGTCTGCCCTCTGGTAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCATTTTTTCTCCGCCCCCTCACg < 2:736871/137‑1 (MQ=255) gCTGCAGTAGCCGAACGCAAGAAGTCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGACGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg < 1:15957117/137‑1 (MQ=255) gCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg < 2:1246399/137‑1 (MQ=255) gCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg < 1:15008243/137‑1 (MQ=255) gCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg < 2:10047211/137‑1 (MQ=255) gCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTGGTCCGGTGCCGGCCTTTTCGCGGCCTTGCCCACGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg < 1:14638229/137‑1 (MQ=255) cggCAGGAGCCGAACGAAAGAAGGCTGCCCTCGGGCATCC‑TTTTTTGTTTTTCTTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGc < 1:9788807/135‑1 (MQ=255) cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTGATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGc < 2:10737718/137‑1 (MQ=255) cAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAAACTCAGTTTTTCTCCGCCCGCTCACGCAGc > 2:17580243/1‑137 (MQ=255) ggaGCCGAACGCAAGAAGGCTGCCCTCGGGCAG‑CTTTTTTTATTTTTCCTGTGCCGGCCTTTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc < 1:1452590/135‑1 (MQ=255) gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTTTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc < 1:8423342/137‑1 (MQ=255) gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc < 2:14772506/137‑1 (MQ=255) gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc < 2:15673570/137‑1 (MQ=255) tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGTCTCTTCTTGGGCTTGCCCGCGGAGGGGCCTGTACAGACA‑CCCCAAAACCTCAGTTTTTTTCCGCCCGCTCACGCAGCCCt > 1:16854228/1‑137 (MQ=255) tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCACGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCACTCACGCAGCCCt > 2:10267790/1‑137 (MQ=255) attcGAACGCAAGAGGGTTGCCCTTGGGAAGCC‑TTTTTTATTTTTCCTGTGCCGGCCTCTTCGTGTCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt < 1:5616420/134‑1 (MQ=255) aGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACNAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt < 1:18285342/137‑1 (MQ=255) aGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTCCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt < 1:4028535/137‑1 (MQ=255) aGCCGAACGAAAGAAGGCTGCCCTCGGGCAG‑CTTTTTTTATTTTTCTTGTGCCGGCCTCTTTGTGTCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCACCCCtt < 1:648189/137‑1 (MQ=255) aGCCAAACAAAAGAAGGCTGCCCTTGGGCATCC‑TTTTTTATTTGTCCTGTTCCGGCCTCTTCGCGGCCTTGCCCGAGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt < 1:256510/137‑1 (MQ=255) ccGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGGCTCTTCGCGGCCTTGCCCACGAAGAGGCCGGCACAAACA‑CCACAAAAACCCCATTTTTCTCCTCCCGCTCCCACAACCCCTTc > 1:8158165/1‑137 (MQ=255) ccGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTc < 2:16442499/137‑1 (MQ=255) gAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCTCGGCCTTGCCCCCAAAGAGGCCGGCACAGACACCCCCAAAACCCCA‑TTTTTCTCCCCCCCCTCCCGCCACCCTTTccg > 1:2260316/1‑135 (MQ=255) aaCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAAAATCAGTTTTTCTCCGCCCGCTCACGCAAACCTTTCAgg > 2:15263532/1‑137 (MQ=255) agaggGCTGCCTTTGGGTATCC‑TTTTTTGTGGGTCTTGTGCTGGCCTTTTCTTGGCCTTTCCCATGAAGGGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCTGCTCACGCAGCCCTTTCAGGGTAtgg < 1:12734484/137‑3 (MQ=255) agaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGACCGGCACAGACA‑CCACAAAAACTCAGTTTTTCCCCGCCCGCTCACGCAGCCCTTTCAGGGTATTg > 1:13238478/1‑137 (MQ=255) aaaagGCTGCCCTCGGGCAGCC‑TTTTTTGTTTGTCCTGTGCCGGCTTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTg < 1:9854239/135‑1 (MQ=255) agGCTGCCCTCGGGCAG‑CTTTTTTGATTTGTCCTGTGCCGGCCTCTTCGTGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAAc < 1:15656607/137‑1 (MQ=255) gCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgcgc < 2:3952208/137‑1 (MQ=255) gCCCTCGGGCAGCC‑TTTTTGATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgcgc < 2:11017753/137‑1 (MQ=255) ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCAt > 2:8888866/1‑137 (MQ=255) ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAAAGGCCGGCACAAACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCAt > 2:5401096/1‑137 (MQ=38) ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCCTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCCGGGTATTGAACAGCGCAt > 2:3784112/1‑137 (MQ=255) cTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGATCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc > 2:16282162/1‑137 (MQ=255) tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTTTCGGACTTTCCCGCGAAGAGGCCAGCACAAACA‑CCCCAAAAACTCAGTTTTTCTCCTCCCCCTCACGCAGCCCTTTCAGGGTATTGAACGGTGCATcc > 1:10624260/1‑137 (MQ=255) tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAAAATAAGTTTTTCTCCGCCCGCTCAAGCAGCCCTTTCAGGGTATTGAACGGCGCATcc > 2:8130975/1‑137 (MQ=255) tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCAGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATcc > 2:17997364/1‑137 (MQ=255) tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGACGGAACAAACA‑CCCCAAAACCCCCATTTTTCTCCTCCCACCCCCACAACCCCTTCAGGGTATTGAAAGGCGCATcc > 1:5882407/1‑137 (MQ=255) cGGGCAGCC‑TTTTTTATTTTTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATcca < 2:14563900/137‑1 (MQ=255) gccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGa < 2:16054426/134‑1 (MQ=255) cttttttGTTTGTCCTGTGCCGGCCTCTTCGCGGCCTTTCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAAc < 1:17998128/136‑1 (MQ=255) ttttttATTTGTCCTGTGCCGGCCTCTTCTCGGCCTTGCCCGCGAAGAGGCCGGAAAAAAAA‑CCCAAAAAACTCAGTTTTTCTCCGCCCGCTCCCACCACCCCTTCAGGGGATTTAACAGAGCATCCACCACGaaat > 1:3773505/1‑135 (MQ=255) ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCCTTCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAAACCCAATTTTTCTCCGCCCGCTCACGCAGACCCTCCAGGGTACTGAACGGCGCATCCACCACAAACt > 1:8903455/1‑137 (MQ=255) tttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACTGCGCATCCACCACGAACtt > 2:6367242/1‑137 (MQ=255) tttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGAAA‑CCACAAAACCTCAGTTTTTTTCCGCCCGCTCAAGCAGCCCTTTCAGGGTATTGAACGGCGCATCCCCCACGAACtt > 1:4377187/1‑137 (MQ=38) tttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCCTGCCCGCGAAGAGGCCGGCACAGACA‑CCCCAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTGTTGAACGGTGCACCCAACACGAACtt > 2:11499457/1‑137 (MQ=255) | AGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTT > AE015451_phaM1/4740690‑4740943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |