BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	5,097,240	0	A	C	30.9%	207.8 / 12.9	145	intergenic (+231/+61)	PP_mr66 ncRNA (P6)/acsA‑I CDS (acetyl‑CoA synthetase)	–/–
Reads supporting (aligned to +/- strand): ref base A (88/8); new base C (43/0); total (136/9)
Fisher's exact test for biased strand distribution p-value = 5.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 148 total aligned reads displayed.

GAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAG  >  AE015451_phaM1/5097109‑5097376

gAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCGAATGCATGAGGTCC‑GGGGGCAACTTAGCCtt                                                                                                                                      >  1:1946317/1‑136 (MQ=255)

  aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCTTGAGGTCA‑GGGGACAACTTCGCCTGaa                                                                                                                                    >  1:3016222/1‑137 (MQ=255)

  aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGCAACCNATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCG‑GGGGAAAGCTTAGCCCGaa                                                                                                                                    >  1:15598470/1‑137 (MQ=255)

    cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGAACGCGTGAGGTCA‑GGGGACAGCCTCGCCTGAAcc                                                                                                                                  >  1:14327353/1‑136 (MQ=255)

    cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGACAGCTTAGCCTGAACt                                                                                                                                  >  2:12115297/1‑137 (MQ=255)

         cGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGACAGCTTCGCCTGAACTGACGc                                                                                                                             >  1:15741116/1‑137 (MQ=255)

         cGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGACAGCTTAGCCTGAACCGACGc                                                                                                                             >  1:15438453/1‑137 (MQ=255)

          ggAATTGTTTCCTGGCGCCCCGAACCTGTGTGGGGCTGGGGACAGAACATTAACAGATGATTTTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCa                                                                                                                            <  1:9036910/137‑1 (MQ=255)

          ggAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGGTTTCCCCCGAAGGCGTGGGGTCA‑GGGGAAAGATTCGCCTCAACTGAcacc                                                                                                                            >  1:6564336/1‑134 (MQ=255)

          ggAATTGTTTCCTGGCGCCCCAAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCa                                                                                                                            <  1:11491072/137‑1 (MQ=255)

            aaTTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGGTTGCCCGATAACGCGTGGGGTCA‑AAGGGCAGCCTCGCCTAGACTTACGCAtt                                                                                                                          >  1:1705187/1‑137 (MQ=255)

               tGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGACCGCTTAGACTGAACTGACGCATTcgc                                                                                                                       >  2:2852750/1‑137 (MQ=255)

               tGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGACAACTTAGCCTGAACCTACGCATTcac                                                                                                                       >  2:13708551/1‑135 (MQ=255)

                    ccTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGAAAGCTTAGCCTGAAATGACGCATTCGCGGG‑Ca                                                                                                                 >  2:11296305/1‑137 (MQ=255)

                    ccTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCACGAATGCGTGAGGTCA‑GGGGAAAGCTTAGCCTGAACTGACACATTCGCGGG‑Ca                                                                                                                 >  2:15661311/1‑137 (MQ=255)

                           ccccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTTTCCCCGAACGCGTGAGGTTA‑GGGGGAAGACTAGACTGAAACGACGCACTCGCGGG‑AAAGaccac                                                                                                          >  1:253624/1‑135 (MQ=255)

                            cccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGGGTGAGGTCA‑GGGGAAAGCTTGGCATGAACTGACGCATTCGCGGG‑CAAGACCACt                                                                                                         >  2:2930065/1‑137 (MQ=255)

                              cGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGTGGATGTGTGAGGTCA‑GGGGACCGCTTCGCCCTAACCTACTCACTCTCAGG‑CAAAcccccccc                                                                                                       >  2:11943368/1‑132 (MQ=255)

                              cGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGGATGCGTGAGGTTA‑GGGGGCAGCCTCGCCTGAACCGACGCCCTCTCTGG‑CCAGCCCCCTcc                                                                                                       >  1:12472778/1‑137 (MQ=255)

                                         ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGACAGCTTCGGCTGAACTTACGCACTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTa                                                                                            >  2:7675078/1‑137 (MQ=255)

                                         ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGACAGCTTCGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTa                                                                                            >  2:10452139/1‑137 (MQ=255)

                                         ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGACAGCTTCGACTGAACTGACGCCCTCGCGGG‑CAAGCCCGCTCCTGCAggggggc                                                                                            >  2:10109580/1‑135 (MQ=255)

                                         ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGACAGCTTAGCCTGAACTTACGCATTCCCGGG‑CAAACCCTCTCCCTCCGGGGGtg                                                                                            >  2:5103490/1‑136 (MQ=255)

                                         ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCAGGAATGAGGGAGGTCA‑GGGGAAAAACTCGCCCGAACCAAAACACTCGCAGG‑AAAACCCGCTCCTGCAaggggga                                                                                            >  2:5103489/1‑135 (MQ=255)

                                         ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCACAAATGCGGGAGGTCA‑GGGGACAGCTTCGCCCGAACTGACGCATTCGCGGG‑CCAACCCACTCCTGCAgagggca                                                                                            >  1:13136938/1‑135 (MQ=255)

                                         ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTGCCCGCGGATGTGTGAGGTTC‑GGGGGAAGCCTAGCCTGAACCGGAACATTTGCGGG‑CAAGTCCGCTCCTGTAgggggga                                                                                            >  1:10782653/1‑135 (MQ=255)

                                         ggggCCGGTGACAGAACAGTAACCGATGATTATCGGGTAACAATGCAAAAGCGGGCTTGCCCGCAAATGCGTGGGGTCA‑GGGGACAGATTCGGCTGAACTGACGCCCTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTa                                                                                            >  1:14512593/1‑137 (MQ=255)

                                             ccGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGTCCGCGAATGCGTGAGGTCA‑GGGGACAACTTAGCCTGAACTGAGGCATTCGCGGG‑CAAGCCCGCTCCTGTAGGGGGGATTGc                                                                                        >  2:3576517/1‑137 (MQ=255)

                                              cGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCAAATGCGTGAGGTCA‑GGGGACAAATTCGCCTTAACTGACGCCCTCACGGG‑CCAACCCACCCCTTCAGGGGGTAttttc                                                                                       >  1:8660110/1‑134 (MQ=255)

                                              cGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTTCCCGCGAACTGGTGAGGTTA‑GGGGGCCGCTTGGCCCAAAACGACGAAGTCTTGGG‑CAAGCCCTCTCCTGTCGGGGGTATTgtc                                                                                       >  1:1757137/1‑135 (MQ=255)

                                              cGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTGCCCGCGAATGCGTGAGGTCA‑GGGGAAAGCTTCGCCTGAACTGACGCACTCTCGGG‑CAAACCCGCTCCTGTAGGGGGTATTGcc                                                                                       >  1:16146296/1‑137 (MQ=255)

                                                gTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTTCCCACGAACGCGTGAAGTCC‑AGGGACAACCTCGCCCGAAATGACGCATTCGCGGG‑CAAGCCCACCCCTGCAGGGGGTATTGCCgg                                                                                     >  2:2122161/1‑137 (MQ=255)

                                                   aaaGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGGGAATGCGTGAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCATTCGAAGG‑CAAGCCCACTCCTGCAGGGGGTATTGCCGGGaa                                                                                  >  2:8404235/3‑137 (MQ=255)

                                                   aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGGTTGCCCGCGAATGCGTGAGGTCA‑GGGGGAAGATTCGCCTGAACTGACGCATTCGCGGG‑CAAACCCGCCCCCTCAGGGGGTATTGTCgggga                                                                                  >  1:10181942/1‑135 (MQ=255)

                                                   aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTTCCGTGGATGCGGTAGGTTC‑GGGGACAGATTGGCCTTAACTGGCGAATTCGCTGG‑AAAGCCCGCTCCTGCAGGGGGTATTGCCGGGaa                                                                                  >  1:58723/1‑137 (MQ=255)

                                                   aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCACGAATGCGTGAGGTCA‑GGGGAACACTTCGCCTTAACCGACGCATTCGCGGG‑CAAGCCCACCCCTGCAAGGGGTATTGCCGGGaa                                                                                  >  2:16703060/1‑137 (MQ=255)

                                                   aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGACAGCTTCGCCTGAACTGACGCATTCTCGGG‑CAAGCCCGCTCCTTCAGGGGGTATTGCCAGGaa                                                                                  >  2:15028631/1‑137 (MQ=255)

                                                   aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGACAGCTTAACCTGAACTTACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGaa                                                                                  >  2:8487335/1‑137 (MQ=255)

                                                   aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGACAGCCTCGCCTGAACTGACGCATTCGCGGG‑CAAGCCCACTCCTGCAGGGGGTATTGCCGGGaa                                                                                  >  2:8487336/1‑137 (MQ=255)

                                                   aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGAAAGCTTAGACCGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGaa                                                                                  >  2:8404234/1‑137 (MQ=255)

                                                   aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑AGGGAAAGATTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTACTGCCGGGaa                                                                                  >  2:14137776/1‑137 (MQ=255)

                                                   aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAACGCATGAGGTCA‑GGGGACAGCTTAGCCTGAACTGACCCATTCGCGGG‑CACACCCGCTCCTGCAGGGGGTATTGCCGGGaa                                                                                  >  1:16170920/1‑137 (MQ=255)

                                                   aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTGTCCTCAAAGGTGTTGGGGCAGGGGGAAAATTCACCCGGAACT‑ACGGATCTTCGGG‑GAAGCCCTCTCCTTCACGGGGTGTTGTCTGGaa                                                                                  >  1:9540188/1‑137 (MQ=255)

                                                        aCAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCACGAATGCGTGAGGTCA‑GGGGAAAACTTAGCCCGAACTGACGCATTCGCGGG‑CAAACCCACTCCCTCAGGGGGTATTGCCGGGAAGGGtt                                                                             >  1:13943404/1‑137 (MQ=255)

                                                         cAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGAAAGCTTAGCCTGAACCGAAACATTCTCGGG‑AAAGCCCACTCCTGCAGGGGGTATTGCCGGGAAGGGttt                                                                            >  1:6790279/1‑137 (MQ=255)

                                                             aaCCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGAATGCGTGAGGTCA‑GGGGACAGCTTCACCCTAACTGACACATTCTCGGG‑CCAGCCCACTCCCGCAAGGGGTATTGCCGGGGAGGGTTTCCGc                                                                        >  1:13158667/1‑137 (MQ=255)

                                                              aCCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCTCGAATTCGTGAGGTCC‑GGGGACCGATTAGCCTGAACCGACGCCTTCCCGGG‑CAAGCCCACTCCTGCCAGGGGTACTGCCGGGAAGGGTTTACGcc                                                                       >  1:15323873/1‑137 (MQ=255)

                                                               ccGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGAAAACTTCGCCCGAACCGACGCATTCGCGGG‑CAAGCCCGCTCCTTCAAGGGGTATTGCCAGGAAGGGTTTCCGcca                                                                      >  1:11273638/1‑136 (MQ=255)

                                                               ccGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAACGCGTGAGGTCA‑GGGGAAAACTTCGCCCAAACTGACACACTCTCGGG‑CAAGCCCACTCCTTCCGGGGGTATTGCCGGGAAAGGtttccctcc                                                                      >  1:14570110/1‑131 (MQ=255)

                                                               ccGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCAAATGCGTGAGGTCA‑GGGGACAACCTCGCCCGAACCGACACACTCGCGGG‑CAAGCCCACTCCCTCCGGGGGTATTGCCGGGAAGGGTTTCCGcct                                                                      >  1:11974155/1‑136 (MQ=255)

                                                               ccGATGATTGTCGGGTAACAATGCAAAAGCGGGACTGCCCACGAATGCGTGAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCACTCGCGGG‑CAAGTCCGCTTCTGCAGGGGGTCTTGCCGGGAAGGGTTTACGCCg                                                                      >  2:13758630/1‑137 (MQ=255)

                                                                 gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCACGAATGCGTGAGGTCA‑GGGGACAGCTTCGACTGAACTTACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCAGGAAGGGTTTCCGCCGaa                                                                    >  2:13223092/1‑137 (MQ=38)

                                                                      ttGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGAATGCGTGAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAACCCGCTTCTTCAGGGGGTATTGCCGGGAAGGGTTTCCGCCAAAGCCAg                                                               >  2:4774805/1‑137 (MQ=255)

                                                                      ttGTCGGGTAACAATGCAAAAGCGGGATTGCCCGCGAATGCGTGAGGTCA‑GGGGACAGTTTAGCATGAAATGACGCATTCGCGGG‑CAAACCCACTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGGCAg                                                               >  2:5356052/1‑137 (MQ=255)

                                                                       tGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGGGGTCA‑GGGGAAAGCTTAGCCTGAACTGACGCCTTCTCGGG‑CAAGCCCACTCCTTCAGGGGGTATTGCCGAGAAGGGTTTCCGCCAAAACCaag                                                              >  1:1077795/1‑135 (MQ=255)

                                                                       tGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAACGCGTGGGGTTA‑GGGGACAACCTAGCCTGGACCGACGCATTCTGGGG‑CAAGGCCACTCCTGCAAGGGGTATTGCCGGGAAGGGTTTACGCCGAAACCaag                                                              >  1:12974886/1‑135 (MQ=255)

                                                                                      cAAAAGCGGGCTTGCCCGCGAATGCATGCGGTCA‑AGGGACAGCTTAGCCTGAACCGACGAATTCGCAGG‑CAAACCCGATTCCGCAAGGGGTATCGCCGGGGAGGGCTTCCGACTCAGCCAGGTTCCTCCCCTTGTgg                                               >  2:16205764/1‑137 (MQ=255)

                                                                                      cAAAAGCGGGATTGTCCCCAAATGCGTGAGGTCA‑GGGGAAAGCTTCAACTGAAACGACGCAGTCGCGGG‑CAAACCCACTCCCGCAAGGGGTACTGCCAGGAAGGGTTTCCCCCCAAACCCAGTTCCTCACCTTGTgg                                               >  2:5687616/1‑137 (MQ=255)

                                                                                       aaaaGCGGGATTGCCCGCGAATGCGTGAGGTCA‑GGGGACAGCTTCGCCCGAACTGAAGCATTCGCGGG‑CAAACCCGCTTCCTCAGGGGGTATTGCCGGGAAGGGTTTCCGCCAAAGCCAAGTTCCTCGCCTTGTggg                                              >  2:10642626/1‑137 (MQ=255)

                                                                                                 ttGCCCGCGAACGCGTGGGGGCA‑GGGGAAAGCTTCGCCTGAACTGACGCATTCTCGGG‑GAAGCCCGCTTCTGGAGGGGGTATTGCCGGGAAGGGTTTCCGCCGAAGCCAGGTTCATCGCCTTGTGGGTGTCGATCAg                                    >  2:2156337/1‑137 (MQ=255)

                                                                                                      cgcgAATGCGTGAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCCTTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCt                               >  2:16519729/1‑137 (MQ=255)

                                                                                                           aTGCGTGAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTAcc                          >  1:10655774/1‑137 (MQ=255)

                                                                                                            tGCGTTAGGTCA‑GGGGACAGCTTAGCCTGAAATGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGATTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAAGTGTTGTACca                         >  2:11901834/1‑137 (MQ=255)

                                                                                                            tGCGTGAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACca                         >  2:11608906/1‑137 (MQ=255)

                                                                                                                 gAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCATTCGCGGG‑TAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATTAGGTGCTGTACCACAccc                    >  2:14193356/1‑137 (MQ=255)

                                                                                                                 gAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                    >  2:3143203/1‑137 (MQ=255)

                                                                                                                 gAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                    >  2:3143248/1‑137 (MQ=255)

                                                                                                                 gAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                    >  2:6527550/1‑137 (MQ=255)

                                                                                                                 gAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                    >  2:13379493/1‑137 (MQ=255)

                                                                                                                 gAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                    >  1:11741397/1‑137 (MQ=255)

                                                                                                                 gAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                    >  1:11798602/1‑137 (MQ=255)

                                                                                                                 gAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                    >  1:2314704/1‑137 (MQ=255)

                                                                                                                 gAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                    >  1:6498900/1‑137 (MQ=255)

                                                                                                                 gAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                    >  1:9678343/1‑137 (MQ=255)

                                                                                                                  aGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                   >  1:4526116/1‑137 (MQ=255)

                                                                                                                   ggggtgggggACAGTTTGGTCGGAGGTGACCCGTTCGGGGGCCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                    <  2:15886796/131‑1 (MQ=255)

                                                                                                                   gggagggggaACAGCTCAGCCCGACTCCACGCGTTCGCGGA‑CAATCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                   <  2:14736689/131‑1 (MQ=255)

                                                                                                                           ggACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGcc           >  1:1005125/1‑137 (MQ=255)

                                                                                                                           ggACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGcc           >  1:1005126/1‑137 (MQ=255)

                                                                                                                           ggACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGcc           >  2:7824852/1‑137 (MQ=255)

                                                                                                                           ggACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGcc           >  1:13924772/1‑137 (MQ=255)

                                                                                                                             aCAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa         >  1:1472471/1‑137 (MQ=255)

                                                                                                                             aCAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa         >  1:13059944/1‑137 (MQ=255)

                                                                                                                             aCAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa         >  1:2273889/1‑137 (MQ=255)

                                                                                                                             aCAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa         >  1:4406713/1‑137 (MQ=255)

                                                                                                                             aCAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa         >  2:2641170/1‑137 (MQ=255)

                                                                                                                             aCAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa         >  1:5731137/1‑137 (MQ=255)

                                                                                                                             aCAGCTTAGCATGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa         >  2:5305199/1‑137 (MQ=255)

                                                                                                                                 cTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTc     >  1:1097058/1‑137 (MQ=255)

                                                                                                                                  ttAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCg    >  2:10017743/1‑137 (MQ=255)

                                                                                                                                  ttAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCg    >  2:10145772/1‑137 (MQ=255)

                                                                                                                                  ttAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCg    >  1:73833/1‑137 (MQ=255)

                                                                                                                                  ttAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCg    >  1:15227146/1‑137 (MQ=255)

                                                                                                                                   tAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga   >  2:7688304/1‑137 (MQ=255)

                                                                                                                                   tAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga   >  1:1868560/1‑137 (MQ=255)

                                                                                                                                   tAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAACCAAGGTCga   >  2:15740853/1‑137 (MQ=255)

                                                                                                                                   tAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGATCAGCCAAGGTCga   >  2:9163553/1‑137 (MQ=255)

                                                                                                                                   tAGCATGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAAGGTTTACGCCGAATCCAGGTTCATCGACTTGTGGGTATCGATCAGGTGCTGTACCACACCGGGATCAGCCAAGGttaa   >  2:9163523/1‑134 (MQ=255)

                                                                                                                                   tAGCATGAAATGACGCATTCGCGGG‑CAAGCCCGCTCATGCAGGGGGTATTGCCGGGAAGGGTTTACGTCGACGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga   >  2:8064185/1‑137 (MQ=255)

                                                                                                                                    aGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgag  >  2:12560724/1‑137 (MQ=255)

GAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCA‑GGGGACAGCTTAGCCTGAACTGACGCATTCGCGGG‑CAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAG  >  AE015451_phaM1/5097109‑5097376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`