| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AE015451_phaM1 | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | trpS CDS (Tryptophan‑‑tRNA ligase) → / → zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451_phaM1 | 1,499,480 | 0 | C | A | 92.2% | 408.6 / NA | 194 | intergenic (+57/‑107) | trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Reads supporting (aligned to +/- strand): ref base C (14/1); new base A (106/71); total (120/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | AE015451_phaM1 | 1,499,481 | 0 | A | C | 97.4% | 463.3 / ‑6.3 | 195 | intergenic (+58/‑106) | trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base C (116/71); total (120/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 195 total aligned reads displayed.
GTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTG‑T‑T‑T‑CGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAG > AE015451_phaM1/1499351‑1499611 || tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTg < 1:3964874/137‑1 (MQ=255) tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTg < 2:7854379/137‑1 (MQ=255) tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTg < 2:16323413/137‑1 (MQ=255) cGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGc < 1:15831770/137‑1 (MQ=255) acgaCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCTTTGGCACCACAGCATGACTGGGGCTGCCAAACGTTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg < 2:14902189/134‑1 (MQ=37) cAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCGCGAAACAGGCGCCGCGGTGTCTGGCACCAGATTTGCCgg > 2:427895/1‑137 (MQ=255) cAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAACGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGcctg > 2:9720241/1‑135 (MQ=255) cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgtg‑t < 2:668486/137‑1 (MQ=255) cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgtg‑t < 1:9424266/137‑1 (MQ=255) cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCAGCTTTGCCGgtg‑t > 2:4809709/1‑137 (MQ=255) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCCCGAAACAAGCGCCACAGTTCATTGCCCCAGCTTTTCCCGTG‑t‑t‑tt > 1:4971436/1‑136 (MQ=255) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCACGCAAAAGGAGCCTCAGGGCATGGCCCCAGATTTTCCGGTG‑t‑tct > 1:13026964/1‑135 (MQ=255) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCACGAAACAAGCGCCACGGTGCCTGGCACCGGCTTTGCCTGTG‑t‑tct > 1:11701029/1‑135 (MQ=255) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCGCGGTGCACGGCACCGGCTTTGTCGGTG‑t‑tcg > 2:6301126/1‑135 (MQ=37) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCATGCCCGCAAAACAGGCGCCGAGGTGCATGGCACCGGCTTTGCCGGTG‑t‑tcg > 2:150466/1‑135 (MQ=38) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAAGCGCCACGGTGCACGGCACCGGATTTTTCGGTG‑t‑t‑tt > 1:2853386/1‑136 (MQ=255) aaGCCCGTGATGTGGCCATTCAGAGCCTGAACGAAGCATTGGCACCACCGCAGGACTGAGGCTGCCAAACGGTAGAAGCGGGCGTGTCCACGGAACAGGCGCCACCGTGCACGGCACCAACTTTGCCGGTG‑t‑tcgcg > 2:6108530/1‑133 (MQ=255) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGCCCGAGAAAAAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAa‑g‑cc > 2:2441653/1‑137 (MQ=25) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACCGGATTTGCCGGTG‑T‑TCG‑CGGGCAc‑g‑cc > 2:16218334/1‑137 (MQ=25) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCCCTGCTTTGTCGGTG‑T‑TCG‑CGGGCAc‑g‑cc > 2:10817124/1‑137 (MQ=37) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAGGCGCCACGGGGTATGGCACCCGCTTTTTCCGTG‑T‑TCT‑CGGGCAcaac > 1:5258733/1‑134 (MQ=255) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAAGCGCCACAGTGTATGGCACCAGCTTTGCCGGTG‑T‑TCT‑CGGGCAc‑a‑cc > 2:14170624/1‑137 (MQ=25) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAAAAAGAGCCGCGGTGCATGGGACCAGATTTTCCGGTG‑T‑TCT‑TGGGCAc‑a‑cc > 1:10175655/1‑137 (MQ=255) aTGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTTCCCGCGAAACAGGCGCCGCGGGGCATGGCACCGGCTTTGCCAGTG‑T‑TCG‑CGGGCAc‑g‑ccc > 2:9600394/1‑136 (MQ=37) aTGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACCGGCTTTGTCGGTG‑T‑TCG‑CGGGCAc‑g‑ccc > 2:3984472/1‑136 (MQ=25) tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGGCCGCGAAACAAGAGCCACGGTGCCTGGCCCCGGGTTTTCCGGTGTT‑T‑T‑CGGGCAc‑gaccg > 1:4777954/1‑133 (MQ=255) ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTccc > 2:6751384/1‑137 (MQ=21) ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGTCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTccc > 2:3017836/1‑137 (MQ=21) ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTccc > 2:10071397/1‑137 (MQ=21) ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCGCGGTGCATGGCACCGGGTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTccc > 2:15297598/1‑137 (MQ=18) ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCAAAACAAGCGCCATGGTGCACGGCACCAGCTTTTTCGGTGTT‑T‑T‑CGGGCAC‑GCCCGCTccc > 1:13872817/1‑137 (MQ=255) gatccTGAACGAAGCTTTGGCACCACAGCAGGACGGAGGCTGCCAAGCGGTGGGAGCGGGCGTGCCCGTGAAACATGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGACAC‑GCCCGCTCCCACAGACt < 2:15394393/134‑1 (MQ=17) gagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCACGGTGCATGGCACCAGTTTTGCCGGTG‑T‑TCG‑CAGGCACAC‑CCGCTCCCAAAGACt > 2:9820211/1‑137 (MQ=21) gagCCGGAACGAGGCCTTGGCACCACAGCAGGACTAAGGCTGCCAAACGGTGAGAGCGGGCGTGCCATCGAAACAGGCGCCGCGGTGCATGGCACCGTCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACt < 2:5469515/137‑1 (MQ=18) gAACGAAGCCTTGGCACCACCGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCACTCCCCCAGAATGATTGc > 2:7079614/1‑137 (MQ=18) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGc > 2:10242089/1‑137 (MQ=21) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGAATGATTGc > 2:3069670/1‑137 (MQ=21) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCAAAGACTGATTGc > 2:12613622/1‑137 (MQ=21) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCAAAAAAGGCGCCACGGTGCCTGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAAACTGATTGc > 1:14132766/1‑137 (MQ=18) gaggtcTTGGCACCACAGAAGGACGGAGGCTGCAAAATGGTGGGATTGGGCGTTCCCGCGAAACAGGTTCCGTGGGGCATGGAACTGGTTTGGTTGGTG‑T‑TCG‑TGGGCAC‑GCCCGCTCCCACAGACTGATTGCCAtt < 2:8813186/132‑1 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGTGGGCGTGCCCGCGGAACAGGCGCCGAGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGATCCCACGGGCTGAGTGCCagt > 2:11254793/1‑135 (MQ=18) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCAAAACAGGCGCCGCGGGGCAGGGCACCGGCTTTGTCGGTG‑T‑TCG‑CGGGTAC‑GCCCACCCCCACAAACCAATTGCCact > 1:15265554/1‑135 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCACGAAACAAGCGCCGCGGTGCATGGCACCGGATTTGCCGGTG‑T‑TCG‑CGGGGAA‑GCCCGCTCCCACAGAATGAGTGACAtt > 1:6651840/1‑137 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCCCGAAACAGGAGCCACGGTGTCTGGCCCCAGCTTTGCCGGTG‑T‑T‑TGCGGGCCCAC‑CCACTCCCCCAGACCGGTTGCCact > 1:13507491/1‑135 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGTGCCGCGGTGCATGGCACCAGATTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCACTCCCACAGAATGACTGCCact > 2:5352466/1‑135 (MQ=18) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCact > 2:3671859/1‑135 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCAtt > 2:7218445/1‑137 (MQ=18) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCACCACGGTGCCTGGCACCCGCCTTTCCGGTG‑T‑TCT‑CGGGCACAC‑CCGCCCCCACAAACCGATTTcccct > 2:5264328/1‑134 (MQ=16) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAAGCGCCGCAGTGCATGGCACCAGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCACCCCCACCGAATGATTGCCact > 1:12233814/1‑135 (MQ=17) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCAAAATGGTTGAAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCGGGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCAtt < 2:15150973/137‑1 (MQ=18) gAAGCCTTGGCACCAAAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGGGCCGCGGTGCCTGGCCCCGGCTTTGCCGGTG‑T‑T‑TGCGGGCCCTC‑CCCCTCCCACAGACTGATTGCCact > 2:3046908/1‑135 (MQ=21) ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAGGCGCCGCGGTGCACGGCACCAGGTTTGCCAGTG‑T‑TCG‑CAGGCAC‑GCCCGCTCCCAAAGAATGATTGCCATTAAGc > 2:2832541/1‑137 (MQ=255) ccTTGGCACCACAACAGGACTAAGACTGCAAAATGGGGGGAGTGGGTGTGCCCGAAAAACAGGCGCCGTGGTGAGTGGAACTGGTTTTGCTGGTG‑T‑T‑CGTGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGc < 1:7558198/137‑1 (MQ=255) tttccaccacaccaGAACGGAGCCTACCACCCGTTGGGAGCGGGCGTCCCCGCGAAACGGGCTCCGCGGTGCATGTCACCGTCTTTGCCGGTT‑T‑TCT‑GGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 2:4892964/133‑1 (MQ=12) ttGGCACCCAACCAGGACTGATCCGGCCAAATGGTGGGAGCGGGCGTACCCGCGACACATGCACCGCAGTCCATAACACCGTCTTTTCCGGTG‑T‑TAA‑AGGACAT‑ACCCGCTCCCACAGACTGATTGCCATTAAGCgg < 2:2015233/137‑1 (MQ=255) ttGGCACCACACCAGGACTGAGGCGGCAAAACGGGGGGAGGGGGCGTGCCCGAGAAAGAGGTGCCGTGGGGCATGGCACCGGTTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 2:6652756/137‑1 (MQ=16) acaccacAGAGGGATGGAGGCGTCCAAAAGGGGGGAGGGGGGGGGCCCGTAAAACGGGAGCCGTGGGGAATGGCATTGGTTTTGCGGGGG‑TGC‑G‑TGGACAG‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 2:2542474/136‑1 (MQ=255) caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGTCCGCGAAAAAAGCGCCACGGTGCCTGGCACCAGCTTTTTCGGTG‑T‑TCG‑CGGGTCC‑GCCCCCTCCCACAAAATGATTGCCACTCAACGGGACt > 1:7001934/1‑137 (MQ=255) caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGTCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCt > 2:6352587/1‑137 (MQ=21) caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAGGCGCCGCGGTGCCTGGCACCAGATTTGCCGGTG‑T‑TCG‑CAGGCAC‑GCCCACTCCCACAGAATGATTGCCATTCAACGGGCCt > 1:10451558/1‑137 (MQ=255) caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGTCCGCGAAACAAGCGCCGCGGTGTACGGCACCGGATTTGCCAGTGTT‑T‑T‑CGGGTAC‑GCCCACCCCCACAAAATGAGTGCCATTGAGAGGGCCt > 1:7288381/1‑137 (MQ=255) ccacAGCAGGACTGAGGCTGCCAAATGGTGGGAGTGGGTGTACCTGCGAAACAGGCACCGCGGTGCGTGGCACCGGTTTTGCCGGTG‑T‑TCG‑TGGACAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTAt < 1:13297130/137‑1 (MQ=255) acaGCAGGACTGAGTCTTCCAAACGGTGGGGGCGGGCGTGCCCGCGAAACAGTCGCCGCGGTGCAGGGCACTGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 2:11701272/137‑1 (MQ=18) aCTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCCCGAAACAAGCGCCACGGTGCCTGGCACCAGCCTTTTCAGTG‑T‑TCG‑CAGGCAC‑GCCCGCTCCCACAAAATGATTGCCACTCAGCGGGACCATCGCTCCAATg > 2:3006359/1‑137 (MQ=12) aCTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAGGCGCCGCGGTGCATGGCACCGGATTTGCCGGTG‑T‑T‑TGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTCAGCGGGACTATCGCTACAGTg > 2:7547334/1‑137 (MQ=21) cTGAGGCTGCCAAACGGTGGGAGCGGGTGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACCGGCTTTGCCGGTG‑T‑TCG‑AGGGCACAA‑CCGCTCCCACAGAATGATTGCCACTCAGCGGGACTACCGATAAAGGGa > 2:5066090/1‑137 (MQ=12) tGAGGCTACCAACCGGGGGGAGTGGGCGTACCCGCGAAACAGGCGCCGCGGTGCATGACACCGGCTTTGCTGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc < 2:13798245/137‑1 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCCCCTGCTTTGTCGGTG‑T‑TCG‑CGGGCCC‑GCCCGCTCCCAAAGACTGATTGCCATTAAGCGGGTCTACCGCTACAGTGACgg > 2:10411353/1‑137 (MQ=21) gttGCCAAACGGGGGGGGCGGGCGTTCCTGTGAAACGTGCGCCGCGGTGCGTGGCACCGGTTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGcc < 2:11912032/135‑1 (MQ=18) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCGCGGTGCATGGCACCGGGTTTGCCAGTG‑T‑T‑TGCGGGACC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc > 2:1680656/1‑137 (MQ=21) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGTGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑TGTGGGGAC‑GCCCGCTCCCACAGGCTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAAGGccc > 2:8942531/1‑137 (MQ=21) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCACGGTGCACGGCACCAGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc > 2:15911107/1‑137 (MQ=21) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGAGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAAAACGATTGCCATTAAACGGGCCTATCGCTACAGTGACGGccc > 2:7192611/1‑137 (MQ=18) cTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAAGCACCGCGGTGCATGGCACCGGATTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCAAAGAATGATTGCAATTAAGCGGGCCGACCACTCAAGTGACGGacc > 2:6453055/1‑137 (MQ=9) cTGCCAAACGGTGGGAGCGGGAGTGCCCGTGAAACAGGCGCCGCGGTGCATGGCACCGGTTTTGTCGGTG‑T‑TCG‑CGGGCAC‑GACCGCTCCCAAAGACTGAGTGCCATTAAGCGGGACTCGCACTACAGTGACGGacc > 2:5531945/1‑137 (MQ=255) gCCAAACGGTGGGAGCGGGCGTGCCCACAAAACAAGCGCCACAGTGCACGGCCCCAGCTTTTCCCGTG‑T‑TCT‑CGGGCACAC‑CCACCCCCCCAAAACGATTGCCACTCAACGGGCCCATCACTCCAATGACAGcccat > 2:4380961/1‑135 (MQ=255) aaaCGGTGGGAGCGGGCGTGCCCGCAAAACAAGAGCCGCGGTGCACGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCCCCGACTGATTGCCATTCAGGGGGACTATCGCCCCCGTGACGGCCCGtttt > 2:5872676/1‑137 (MQ=9) aaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGTATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTCAGCGGGCCTCTCGCTACAGTGACGGCCCGttttt > 2:15598995/1‑137 (MQ=18) aaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGAAC‑GCCCACTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttttt > 2:8667077/1‑137 (MQ=21) tGGGAGCGGGTGTGCCCGCGAAACGGGCGCCGTGGTGCAGGGCACCGGCTTTGCTGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgttg < 2:3317264/137‑1 (MQ=21) gCGGGGGTGCCCGCGGAACAGGCGCCACGGTGCATGGAACCGGCTTTGGCGGTG‑T‑TCG‑CGGGAAC‑GCCCGATCCAACAGGCTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCAATTTTCTGTTGCCTCg > 2:407127/1‑137 (MQ=255) cGGGCTTGCCCGCGAAACAGGTGCCGTGGTGCGTGGCATCGGCTTTGCCGGTG‑T‑T‑TGAGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc < 2:14577654/137‑1 (MQ=25) agggCGTACCAGAGAAACAGGCGCCGTGGTGCAGGGCACCGGTTTTGCTGGTG‑T‑TCG‑CGGACAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc < 1:9071084/136‑1 (MQ=255) ctcgAAAAAGGCGCCGTGTTGCTTTGCATCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:9073355/135‑1 (MQ=25) cgcgAAACAGGCGCTGCGGTGCATGGCACCGGCTTTGCCGGGG‑T‑TCG‑CGGGAAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCTTTTTTTGTTGCCTCGCTAACGAATCa < 2:10674676/137‑1 (MQ=25) cgcgAAACAGGCGCAGCGGTGCATGGCACTGGTTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCAACAGACTGATTGCCATTAAGCGGGCCTATCGTTATAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:13179536/137‑1 (MQ=25) cgcaAAACATTCGCCGCGGTGCGTGCCCCCGTCTTTTCCGTTG‑T‑T‑CGCGGACAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:4407140/137‑1 (MQ=255) cgaaaaaCAGGCGCCGTGGTGCGGGGCACTGGTTTTGCCGGTG‑T‑T‑TGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:200421/133‑1 (MQ=37) cgaaaaaCAGGCGCCGTGGTGCATGGCACCGTCTTTGCTGGTG‑T‑TCG‑CGGACAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGATAACGAATCa < 1:14170624/133‑1 (MQ=255) aaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCAAAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGACTCATGACt > 2:12225911/1‑137 (MQ=25) cgcttcgGTGCATGGCACAAGCTTTGCTGGTG‑G‑TCG‑TGGGCAC‑GCCCGCTCCCACAGACTGATCGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCGCGCTAACGAATCATGACTCCCTTa < 2:9145510/132‑1 (MQ=25) cgccgtGGTGCGGGGCACCGGTTTGGCCGGTG‑T‑T‑TGTGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 1:3796010/137‑1 (MQ=255) cgccggGTTGCGTGGCACCGGTTTTTCGGGTG‑T‑T‑CGCGGGCAT‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:6252241/137‑1 (MQ=25) cgccgcGGTGCGTGGCACCGGCTTTGCTGGTT‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:1061534/137‑1 (MQ=37) cgcagcGGTGCATGGCACTGTCTTTGCGGTTG‑TCC‑G‑CGGACAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:8218430/137‑1 (MQ=255) ccgtGGTGCATGGCGCTGTTTTTGCTGGTG‑T‑T‑TGGGGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGa < 1:10483162/137‑1 (MQ=255) cgGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg > 1:3694123/1‑137 (MQ=37) cgGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg > 2:13207512/1‑137 (MQ=37) gCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt > 1:6109434/1‑137 (MQ=37) cATGGCACCGGCTTTTCTGGGG‑T‑TCT‑CGGGCAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 2:2669011/137‑1 (MQ=255) || GTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTG‑T‑T‑T‑CGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAG > AE015451_phaM1/1499351‑1499611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |