Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM14,353,1930AC29.0% 336.0 / 42.3 276T107P (ACG→CCG) yrpB CDS (2‑nitropropane dioxygenase)
Reads supporting (aligned to +/- strand):  ref base A (183/13);  new base C (79/1);  total (262/14)
Fisher's exact test for biased strand distribution p-value = 7.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 276 total aligned reads displayed.

GCGCCGGCTGCCCGGGGCGCCCGCTGAACCTGAACTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCA  >  AE015451_phaM1/4353062‑4353328
                                                                                                                                   |                                                                                                                                       
gcgcCGGCTGCCCGGGGCGCCCGCTGAACCTGAACTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGgcg                                                                                                                                    >  1:16717206/1‑137 (MQ=255)
gcgcCGGCTGCCCGGGGCGCCCGCTGAACCTGAACTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGgcg                                                                                                                                    >  1:15433095/1‑137 (MQ=255)
  gcCGGCTGCCCGGGGCGCCCGCTGAACCTGAACTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCCCGGCGcc                                                                                                                                  >  1:7204733/1‑137 (MQ=255)
  gcCGGCTGCCCGGGGCGCCCGCTGAACCTGAACTTCTTCTGACACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGcc                                                                                                                                  >  1:10455398/1‑137 (MQ=255)
    cGGCTGCCCGGGGCGCCCGCTGAACCTGAACTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCCCGGCGCCCa                                                                                                                                >  1:11854665/1‑137 (MQ=255)
    cGGCTGCCCGGGGCGCCCGCTGAACCTGAACTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGcccc                                                                                                                                >  1:9002002/1‑136 (MQ=255)
    cGGCTGCCCGGGGCGCCCGCTGAACCTGAACTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCa                                                                                                                                >  2:7379197/1‑137 (MQ=255)
     ggCTGCCCGGGGCGCCCGCTGAACCTGAACTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCAc                                                                                                                               >  2:15442032/1‑137 (MQ=255)
       cTGCCCGGGGCGCCCGCTGAACCTGAACTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCCCGGCGCCCACGc                                                                                                                             >  1:17632074/1‑137 (MQ=255)
       cTGCCCGGGGCGCCCGCTGAACCTGAACTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCAcac                                                                                                                             >  1:8261741/1‑135 (MQ=255)
         gCCCGGGGCGCCCGCTGAACCTGAACTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGTGCCGATTTCCCGGCGCCCACGCCg                                                                                                                           >  1:4876700/1‑137 (MQ=255)
         gCCCGGGGCGCCCGCTGAACCTGAACTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCCCAGCGCCCACGCCg                                                                                                                           >  1:3935796/1‑137 (MQ=255)
                   cccGCTGAACCTGAACTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAAcc                                                                                                                 >  1:6312235/1‑137 (MQ=255)
                       ctgaacctgaacTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCCCGCCGGTTTCCCAccattc                                                                                                             >  1:4374660/1‑133 (MQ=255)
                       ctgaacctgaacTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGAGCCGATTTCCCGGCGCCCCCGCCGGTGTCCACCCATgc                                                                                                             >  1:6451993/1‑137 (MQ=255)
                                   ttcttcTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGAGTCGAGTTCCCGGCGCCCACACCGGTGTTCAAACGTGTGACTTTtgacta                                                                                                 >  1:11223486/1‑135 (MQ=255)
                                    tcttctGCCACCGACCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTAAAGCGAAGTGGGCGCCGACTTCACGGTGCCCACGCCGGTGTCCACCCATGCTCCTTTCAACAAg                                                                                                >  1:13206950/1‑137 (MQ=255)
                                     cttctGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAAACGTGCGCCTTTCGACGAGc                                                                                               >  2:15064357/1‑137 (MQ=255)
                                       tctGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACAGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCag                                                                                             >  1:14116435/1‑137 (MQ=255)
                                        ctGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCCCGGCGCCCACGCCGGTGTCCACCCATGCTCCTTTCGACGAGCaga                                                                                            >  1:7159234/1‑137 (MQ=255)
                                        ctGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCCCGGCGCCCACGCCGGTGTCCAACCATGCGCCCTTCGACGAGCaga                                                                                            >  1:15252196/1‑137 (MQ=255)
                                        ctGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCaga                                                                                            >  2:869954/1‑137 (MQ=255)
                                         tGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCCCGGCGCCCACGCCGGTGTCCCACCATGCGCCTTTCAACGAGCagag                                                                                           >  1:1944427/1‑137 (MQ=255)
                                         tGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCatag                                                                                           >  2:1022735/1‑137 (MQ=255)
                                         tGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCaaag                                                                                           >  1:1979769/1‑137 (MQ=255)
                                         cgCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGACGTGGGTGTCGCTTTCAGGGCGCCCATGCCGGTGTCCCACCGTGCGCCTTTTGACGGGCaaag                                                                                           >  2:6166734/2‑137 (MQ=255)
                                          gCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGc                                                                                          >  2:4869217/1‑137 (MQ=255)
                                             accaGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCCCGCCGGTGTCCAACCATGCGCCCTTCGACGAGCAGAGctgc                                                                                       >  1:14720750/1‑137 (MQ=255)
                                             accaGCCGCCAGCGCCCGATGCCAAGCGCGATACGCGCTGGAAGCAGATACTTGAGCCTTACTACAGCGCAGCGGGCGTCCATTTCCCGGCACCCACGCAGGCGTCCAACCGTGCGCCTTTCAACGGGTAGAGgtga                                                                                       >  2:16922892/1‑136 (MQ=255)
                                                  ccgccAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGCTTTTACGGCGCCCCCGACGGTGTCCAACCTTGTGCCTTTCGACGAGCAGGGTTTCctgct                                                                                  >  1:1807857/1‑137 (MQ=255)
                                                  ccgccAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCctgct                                                                                  >  2:8070770/1‑137 (MQ=255)
                                                  ccgccAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCctgct                                                                                  >  2:1589338/1‑137 (MQ=255)
                                                  ccgccAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAAGCACTTGAGCCTTATTACAGCGAAGTGGGGAACGAATTCAAAGCACCCACGCCGGTGGCCAAACGTGCCCCTTTCTACACACAGGGCTTCctgct                                                                                  >  1:15157889/1‑137 (MQ=255)
                                                     ccAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCAACTTCACAGTGCCCACACCGGTGTCCAACCGTGCGCCTTTCTACAAGCAGAGCTTCCCGCTGGt                                                                               >  1:10324048/1‑137 (MQ=255)
                                                        gcgcCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCCCAGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGAAGAGCAGAGCTGCCTGCTGGTCGa                                                                            >  1:13702862/1‑137 (MQ=255)
                                                        gcgcCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCCCGCCGGTGTCCCACCGTGCGCCTTTCGACGAGCAGAGCTGCCTTCTGGTCGa                                                                            >  1:15943137/1‑137 (MQ=255)
                                                           cccGATACAAAGCGCGATGCACGTGGAAAGCAGCCACTTGACCCTTCCCAAAGAGAAGGGGACTCTGCTTCCCCTCCCCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGc                                                                         <  2:335401/137‑1 (MQ=255)
                                                            ccGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGTGCCTTTCGACGAGCAGAGCTGTCTGTTGGTCGAAgtg                                                                        >  2:11607555/1‑135 (MQ=255)
                                                            ccGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACCTGAGCCTTATTACAGCGAAGCGGGAGCAGAATTCCCGGAGCCCATGCCGGGGTCAAAACGTGCGCACTTAGACGAGCAGGAGTGCCTGCTGGTCGAAGCg                                                                        >  2:8321966/1‑137 (MQ=255)
                                                              gATGCAGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACAGCGCCCACGCCAGTGTCCAAACGTGCGCCTTTCTACGAGCAGAGCTGCCTGCTGGTCGAAGCGtt                                                                      >  1:10991902/1‑137 (MQ=255)
                                                                tGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCATGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTgc                                                                    >  2:17180408/1‑137 (MQ=255)
                                                                  ccGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGAGTTCACGGCGCCCAAGCCCGTGTCCCACCGTTCGCCTTTCGACGAGCCGAGCTGCCTGCTTGTCGAAGCGTTgagc                                                                  >  2:17066554/1‑137 (MQ=255)
                                                                        gcgATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGTCTGCTGGTCGAAGCGTTGCGCCCGGAg                                                            >  2:13092816/1‑137 (MQ=255)
                                                                        gcgATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAg                                                            >  2:15999297/1‑137 (MQ=255)
                                                                         cgATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCACCCACGCCGGTGTCCAAACGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAgg                                                           >  2:7376626/1‑137 (MQ=255)
                                                                         cgATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGACGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAgg                                                           >  2:17007125/1‑137 (MQ=255)
                                                                         cgATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGACTTCACGGCGCCCACGCCGGTTTCCCACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGGAGCATTGCGGCCGGAgg                                                           >  1:12069733/1‑137 (MQ=255)
                                                                         cgATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCAACTTCACAGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGATGCATGCTGGTCGAAGCGTTTCGCCCGGAgg                                                           >  2:6325711/1‑137 (MQ=255)
                                                                         cgATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGAGCCGATTTCCCAGCGCCCACACCGGTGTCCCACCATGCACCTTTCGCCGAGCCAAGCCGCATGCTGGTCGAAGCATTTCGCCCGGAgg                                                           >  1:9282152/1‑137 (MQ=255)
                                                                            tGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGGTTTCAAGGTGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCCGCCTGCTGGTCGAAGCGTTGCGCCCGGAggtgg                                                        >  2:13866136/1‑137 (MQ=255)
                                                                            tGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAggtgg                                                        >  1:5319099/1‑137 (MQ=255)
                                                                             gcgcgcTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGTGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTTGACGAGCAGAGATGCCCGCTGGTCGAAATGTTTCCCCCGGAggtggt                                                       >  1:5961070/1‑137 (MQ=255)
                                                                             gcgcgcTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCCCAGCGCCCCCGCCGGTGTCCAAACGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAACATTGCACCCTGAggtggt                                                       >  1:4698307/1‑137 (MQ=255)
                                                                             gcgcgcTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCATGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAggtggt                                                       >  1:12946398/1‑137 (MQ=255)
                                                                              cgcgcTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGgtggtg                                                      >  2:13362248/1‑137 (MQ=255)
                                                                               gcgcTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCCCAGCGCCCCCGCCGGTGTCCCACCATGCACCTTTCGAAGAGCCGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGa                                                     >  1:9534184/1‑137 (MQ=255)
                                                                               gcgcTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGa                                                     >  2:4087494/1‑137 (MQ=255)
                                                                               gcgcTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGACGATTTCCCGGCGCCCACGCCAGTGTCCAAACGTGCGCCTTTCTACAAGCAGAGCTGCCCGCTGGTCGAAGCATTGCGCCCAAAGGTGGTGa                                                     >  1:1256864/1‑137 (MQ=255)
                                                                                   tgaaaGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATCTCACGGCACCCACACCAGTGTCCAACCGCGCGCCCCTCGACAAGCAGAGCTACACGCTAGTCGAAGTATTGCGCAAGGAGGGGGGGAGCtt                                                 >  2:9263569/4‑137 (MQ=255)
                                                                                              aCTTGAGCCTTATTACAGCGAAGTGGGTGCCGATTTCCCGGCGCCCACAACAGTGTCCCACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCAGAGGTGGTGAGCTTCCATTTCGGcc                                      >  1:3552179/1‑137 (MQ=255)
                                                                                              aCTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAAACGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCAGGAGGTGGTGAGCTTCCATTTCGGcc                                      >  2:15610117/1‑137 (MQ=255)
                                                                                              aCTTGAGCCTTATTACAGCGAAGTGGGAGCCGATTTCCCGGCGCCCACGCCGGTGTCCAAACGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGcc                                      >  1:6236625/1‑137 (MQ=255)
                                                                                               cTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCCCGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCTACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCCTTTCGGCCt                                     >  1:11556121/1‑137 (MQ=255)
                                                                                                  gAGCCTTATTACAGCGAAGTGGGGGCCGATTTCACGGCGCCCACACCGGTGTCCAAACGTGCGCCTTTCGACGAGCAGAACTGCCTGCTTGTCGAAGCGTTGTGCCCGGAGGTGGTTAGCTTCCACTTCGGCCTGcc                                  >  1:14498056/1‑137 (MQ=255)
                                                                                                  gAGCCTTATTACAGCGAAGTGGGCGCCGGTTTCCCGGCGCCCACGCCGGTGTCCAAACTTGCGCCTTTCGACGAGCAGAGCTGCCTGATGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGGCTGcc                                  >  2:13171098/1‑137 (MQ=255)
                                                                                                  gAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGcc                                  >  2:8518146/1‑137 (MQ=255)
                                                                                                  gAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAAACGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGGGGTGGTGAGCTTCCATTTCGGCCTGcc                                  >  2:7103643/1‑137 (MQ=255)
                                                                                                  gAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCAGTGTCCAAACGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCTAAGCGTTGCGTCCGGAGGTGGTGAGCTTCCCTTTCGGCCTGcc                                  >  2:14520948/1‑137 (MQ=255)
                                                                                                           taCAGCGAAGTGGGCGCCGATTTCCCGTCTCCCCCGCCGGTTTCCCACCGTTCTCCTTTCGACGAGTAGAGCTGCCTGTTGGTCGAAGCGTTGTGCACGGAGGTGGTGAGCTTCCATTTCGGCCTTCCGCAGGCCGa                         >  2:5206994/1‑137 (MQ=255)
                                                                                                           taCAGCGAAGTGGGCGCCGATTTCCCGGCGCCCCCGCCGGTGTCCCACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTTCGCCCAGAGGTGGTGAGCTTCCCTTTCGGCCTTCCCCAAGACGa                         >  1:2662843/1‑137 (MQ=255)
                                                                                                           taCAGCGAAGTGGGCGCCGATTTCCCGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGTTGGTCGAAGCGTTGCGCCCGGGGGTGGTGAGGTTCCATTTCGGCCTGCCGCAGGCCGa                         >  2:12345494/1‑137 (MQ=255)
                                                                                                           taCAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGa                         >  2:12798303/1‑137 (MQ=255)
                                                                                                           taCAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCCGCCGCAAGCCGa                         >  2:10315948/1‑137 (MQ=255)
                                                                                                           taCAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCATGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGGGCTTCCATTTCGGACTGTCGCAAGACGa                         >  1:16799323/1‑137 (MQ=255)
                                                                                                            aCAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGaa                        >  2:4475246/1‑137 (MQ=255)
                                                                                                            aCAGCGAAGTGGGCGCCAATTTCCCGGCACCCCCGCCAGTGTCCAAACATGCGCCTTTCGACGAGCAGAGCTGCCTGCCGGTCGAAACGTTGCGCCCGGGGGTGGGGAGTTTCCATTTCGGCCTGCCGCAGGCCGaa                        >  1:15808662/1‑137 (MQ=255)
                                                                                                             cggCGAAGTGGGCGCCGATTTCCCGGCGTCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGGGGTGGTGAGCTTCCATTTCGGCCCTCCGCAGGCCgcaa                       >  2:4694938/3‑134 (MQ=255)
                                                                                                             cAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCATGCGCCTTTCGACGAGCAGAGCTGCCCGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGACGAAc                       >  1:8803641/1‑137 (MQ=255)
                                                                                                                cGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAAAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAActgc                    >  2:11543308/1‑137 (MQ=255)
                                                                                                                   aGTGGGCGCCGATTTCCCGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGGGATGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGGGGTGAGCTTCCATTTCGGCCCGCCGCAGGCCGAActgctgg                 >  1:17260877/1‑136 (MQ=255)
                                                                                                                   aGTGGGCGCCGATTTCCCGGCGCCCACGCCGGTGTCCAACCATGCGCCTTTCAACAAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCCTTTCGGCCTGCCGCAAGCCAAACtgctgc                 >  1:3349282/1‑137 (MQ=255)
                                                                                                                   aGTGGGCGCCGATTTCCCGGCGCCCACGCCAGTGTCCAACCGTGCGCCTTTCGACGAGCCGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCAGAGGTGGTGAGATTCCATTTCGGCCTGCCGCAGGCCGAACtgctgc                 >  1:3090275/1‑137 (MQ=255)
                                                                                                                   aGTGGGCGCCGATTTCACGGCGCCCCCGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGGGCTTCCATTTCGGCCTGCCGCAGGCCAAACtgctgc                 >  2:5656547/1‑137 (MQ=255)
                                                                                                                   aGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCCACCGTGCGCCTTTCGACGAGCAGAGCTGCCCGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCACTTCGGCCTGCCGCAGGCCGAACtgctgc                 >  1:15758442/1‑137 (MQ=255)
                                                                                                                   aGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAACGTTGCGCCCGGGGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACtgctgc                 >  2:14328739/1‑137 (MQ=255)
                                                                                                                   aGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAAAGCTGCCCGCTGGTTGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACtgctgc                 >  1:7945271/1‑137 (MQ=255)
                                                                                                                     tGGGGGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGTTGGTCGAAGTGTTGTGCCCGGGGGTGGTGAGCTTCCACTTCGGCCTGCCGCCGGTCGAAATGCTGCAg               >  2:1762025/1‑137 (MQ=255)
                                                                                                                     tGGGCGCCGATTTCCCAGCACCCACGCCGGTGTCCAACCGTGCTCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGTGTTGCGCCCGGAGGTGGTTGGCTTCCATTTCGGCCTGCCGCAGGCAGAACcgctgccg               >  1:7587766/1‑135 (MQ=255)
                                                                                                                     tGGGCGCCGATTTCACGGCGCCCCCGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCTGCCTGCCACAGGCCGAACTGCTGCAg               >  1:416540/1‑137 (MQ=255)
                                                                                                                     tGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAg               >  2:6595815/1‑137 (MQ=255)
                                                                                                                     tGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAg               >  2:7595699/1‑137 (MQ=255)
                                                                                                                     tGGGCGCCGATTTCACGGCGCCCACGCAGGTGTCCAAACGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGACGCAGGCCGGACTGCTGCAg               >  2:11095083/1‑137 (MQ=255)
                                                                                                                     tGGGCGCCGATTTCACAGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGTCTGTTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCAAACTGCTGCAg               >  2:10596730/1‑137 (MQ=255)
                                                                                                                        gccccGATTCCACGACCCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCgg            <  2:9918625/134‑1 (MQ=255)
                                                                                                                          gcCGATTTCAAGGCGCCCCCGCCGGTGTCCAACCGTTCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGTTTCCATTTCGGCCTGCCGCAGGACGAACTGCTGCAGCGGGt          >  2:3647504/1‑137 (MQ=255)
                                                                                                                                tccACGCCCCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGc    <  1:11380381/135‑1 (MQ=255)
                                                                                                                                 ccACGTCCCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGcc   <  1:17543755/136‑1 (MQ=255)
                                                                                                                                 ccACGCCCCCCACGCCGGTGTCCAACCTTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGGGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGcc   <  1:17400983/136‑1 (MQ=255)
                                                                                                                                  cACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCa  >  1:12807772/1‑137 (MQ=255)
                                                                                                                                  cACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCACGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCCGTAGCGGGTAAAGGCCa  >  2:2423682/1‑137 (MQ=255)
                                                                                                                                   |                                                                                                                                       
GCGCCGGCTGCCCGGGGCGCCCGCTGAACCTGAACTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCA  >  AE015451_phaM1/4353062‑4353328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑