Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,344,4860TG28.5% 291.5 / 45.9 211intergenic (+61/‑117)pgi‑II CDS (glucose‑6‑phosphate isomerase)/acsA‑II CDS (acetyl‑CoA synthetase)–/–
Reads supporting (aligned to +/- strand):  ref base T (79/69);  new base G (2/57);  total (83/128)
Fisher's exact test for biased strand distribution p-value = 3.98e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 211 total aligned reads displayed.

AGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGTAAA‑CCCGCC‑TACCAGGCAACGCGTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAA  >  AE015451_phaM1/5344351‑5344620
                                                                                                                                          |                                                                                                                                       
aGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGTAAA‑CCCGCC‑TACCAGGCAACGCGTTACCTCGTa                                                                                                                                        <  1:7614966/137‑1 (MQ=255)
aGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGTAAA‑CCCGCC‑TACCAGGCAACGCGTTACCTCGTa                                                                                                                                        <  1:4835104/137‑1 (MQ=255)
aGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGTAAA‑CCCGCC‑TACCAGGCAACGCGTTACCTCGTa                                                                                                                                        <  1:2503327/137‑1 (MQ=255)
aGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGTAAA‑CCCGCC‑TACCAGGCAACGCGTTACCTCGTa                                                                                                                                        <  1:2101844/137‑1 (MQ=255)
        aGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTTTCGGGGGAA‑ACCACC‑CACCAAGAAAAACATTCCCCCGTAGGGG‑Aggg                                                                                                                               >  1:9810367/1‑137 (MQ=21)
           cAGCACCAAAGACGGTTCCACCCACGCCCTGATCAATGACTGTCGCGGCCGTCACTGCGGTTGATCCGACCTTGTGCCGGCCT‑CTTCGCGGGTAAA‑CCCGCC‑TACCAGGCAACGCGTTACCTCGTGGGAG‑CGGGttt                                                                                                                            <  2:9713509/137‑1 (MQ=255)
                ccGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGTAAA‑CCCGCC‑TACCAGGCAACGCGTTACCTCGTa                                                                                                                                        <  1:8580298/121‑1 (MQ=255)
                ccGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGTAAA‑CCCGCC‑TACCAGGCAACGCGTTACCTCGTa                                                                                                                                        >  2:8580298/1‑121 (MQ=255)
                ccGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGGAAA‑CCCACC‑CCACAAGAAAAGCATTCCCCCGTCGGAG‑AGGGGTTcccca                                                                                                                       >  2:6529819/1‑136 (MQ=25)
                ccGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCCCGAGTAAA‑ACCGCC‑CACCAGGCAAAATGTTACACCGTGGGGG‑AGGGGTTCCCcg                                                                                                                       >  2:850607/1‑137 (MQ=25)
                 cGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGTAAA‑ACCACC‑TCCCCAGCAACGCGTTCCCCCCGCGGAG‑CGGGTTTccccac                                                                                                                      >  2:9382477/1‑135 (MQ=255)
                          ttCCACCCAAGTCCTGACAAACTACTTCCGCGGCCGCCACCACGTTTGACCCGAACTTGTGCCGCCCT‑CTTCGCGGGGAAA‑CCCGCC‑TACCAGGCAACACGTTACCTCGGAGGAG‑CGGGTTGACCCGCGAAGAggcc                                                                                                             <  2:2827565/137‑1 (MQ=255)
                            ccACCCAAGTCCTGATCAACTCCTTCCGCGCCCGCCACCGCGTTTGATCCGAGCTTGTGTCGGCCT‑CTTCGCGGGGAAA‑CCCGCC‑TACCAGGCAACGCGTTACCTTGTGGGAG‑CGGGTTTACCCGCGAAGAggccgg                                                                                                           <  2:12827838/137‑1 (MQ=37)
                               ccaaaGGCCTGACCAACTACTCCCTCGGCCGTCACCGCGTTTGATCCGAGTTTGTGCCGTCTT‑TTTCGTGGGGAAA‑CCCGCC‑TACCAGGCAACGCGTCACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGccc                                                                                                        <  2:11474738/134‑1 (MQ=37)
                                 cAAGGCCTGATCAACTACCTCCCCGACCGCCACCTCGTTTGCTCCAGACTTGTGCCGCCTT‑TTTCGCGGGGAAA‑CCCCCC‑GACCAGGCACCGCTTTCCCTCTCAGGAG‑CGGGTTTACCCGCGAATAGGCCGGCCCAg                                                                                                      <  2:5889495/137‑1 (MQ=255)
                                       cTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGGGAA‑ACCACC‑TCCCCAGAAACACGTTGCCCCATAGGAG‑AGGGGTTCCCCCCGAAAAGGACAGACCAAGTTGaa                                                                                                >  2:968770/1‑137 (MQ=255)
                                           tCAACTATTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGGAAA‑CCCGCT‑GACCAGGCAACGCGTTACCTTGGAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACAcc                                                                                            <  2:6029301/137‑1 (MQ=38)
                                            cAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGTCAA‑CCCGCC‑TCCCAAGCAACGCGTTAACCCGTAGGAG‑AGGGTTTACCCACGAAGAGGCCAGACCAGGTTTAACACCg                                                                                           >  2:16494942/1‑137 (MQ=255)
                                             aaCTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTTCCTGGTGAA‑CCCCCC‑CCCCCAGTAACGCGTTCCCCCGTCGAAGAAGGGTTCCCCCCCAAAAAGACCGCCCCA‑GTTTAACAcccc                                                                                          >  2:5017445/1‑135 (MQ=255)
                                             aaCTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCTCGGGTGAA‑CCCACC‑TCCCAAGCACCGCGTTCCCCCGTGAGAG‑CGGGGTTCCCCCCGAAGAGGCCGGCCCCGGTTTAACAccac                                                                                          >  2:9522432/1‑135 (MQ=255)
                                             aaCTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGTGAA‑ACCGCC‑CCCCCAGCAACACGTTACCTCGTAGGAG‑CGGGGTTCCCCGCGAAGAGGACGGCCCAAGGTGAAAAccac                                                                                          >  2:11807022/1‑135 (MQ=255)
                                             aaCTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGTAAA‑CCCGAC‑CCCCCGGCAACGCGTTCCCTCGTAGGAG‑CGGGTTTACCCGCGGAGAGGCCGGCCCAGGTTTGACACCga                                                                                          >  2:16552961/1‑136 (MQ=37)
                                             aaCTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGTAAA‑ACCCCC‑TCCCCAGAAACATGGTACACCATAAGAG‑CAGGGTTCCCCCCAAAAAGGCCGGCCCCAGTTGCACAccac                                                                                          >  2:10335233/1‑135 (MQ=255)
                                             aaCTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CCTCGCGGGTAAACCCCCCC‑CACCA‑GCAAAACGTTACCTCCTCAGAG‑CGGGTTTCCCCGCCAAAAAGCCGGACCAAGTTGAACAccac                                                                                          >  2:14560556/1‑135 (MQ=255)
                                                tactTCCGCGGCCGCCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGTAAA‑CCCGCC‑TACCAGGCAACGCGTTACCTCGTAGGAG‑CGGGTTTACCCGCGAGGAGGCCGGCCCAGGTTGAACACCGCTcc                                                                                       <  1:8505286/137‑1 (MQ=255)
                                                cactTCTGTGGCTTTCACCGCGGTGGATCCGGGTTTGTGCTGGCTT‑TTTTGTGGGGAAA‑CCCGCT‑GACAGGGCAATGCTTTACCTCGGAGGAG‑TGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTcc                                                                                       <  1:16221016/136‑1 (MQ=16)
                                                cactCCCGCGACCACCACCGCGTTGAATCCGAGTTGGTGCCGACCT‑CTTAGCGGGGAAA‑CCCGCC‑AACAAGGCAACGCGTGACCTCGGAGGAG‑TGGGTTCACCTGCGAAGAGGCCGGCCCAGGTTGAACACCGCTcc                                                                                       <  2:622143/136‑1 (MQ=255)
                                                  ctTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTACTCCGAGGGGAAA‑CCACCC‑CAACAGGCAAAGCATGACCCGAAAGGAG‑GGGGTTTAACACGGAGGAAAAAAGCCCAGGTTGAAAACCGCTccc                                                                                      >  2:11205752/1‑137 (MQ=255)
                                                  ctTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGAGGGTAAA‑CCAACC‑TCCCCGGCAACGCGTTACCCCGCAGGAA‑CGGGTTTACCCGCAAAAAGGCCAGCCCCAGTTGGACACCGCTCCCa                                                                                     >  2:2750727/1‑137 (MQ=255)
                                                    tCCGCGGCCGTCACCGCGGTGGATCCGGGCTTGTGCCGGCTT‑TTTCGCGGGGAAA‑CCCTCC‑TACCAGGCAACGAGTCACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGc                                                                                   <  2:12328539/137‑1 (MQ=38)
                                                     ctgtggCTGTCACTGTGTTGGATCGGAGTTTGTGCAGGCCT‑TTTCGTGGGGAAC‑CCCGCC‑GACAGGGAAACGTGTTACTTTGGGGGAG‑CGGGTTTACCCGAGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCt                                                                                  <  2:10585328/133‑1 (MQ=255)
                                                     ccGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCTGGCCT‑CTTCGTGGGTAAA‑CCTGCC‑TACCAGACAACGCGTTACCTCCTAGGAG‑TGGGTTCACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCt                                                                                  <  2:16359026/137‑1 (MQ=38)
                                                     ccGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCTCGGGTGAA‑ACCCCC‑CCCCAAACCACACATTCCCCCCTCGGAG‑CGGGTTTTCCCACAAAAAAGCCAGCCCAAATTTAACACCACTccccacc                                                                                  >  2:7773501/1‑133 (MQ=255)
                                                       gcgGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCTCGGGTGAA‑CCCGCC‑TACCCGGCAACGCGGTACCTCGTCGGAA‑CGGGTTTACCCACAAAGAGGACGGACCAAGGTGAACACAACTCCCaaatac                                                                                >  2:16487208/1‑132 (MQ=255)
                                                       gcgGCCGTAACAGTGGTGGACCCGAATTGGTGCGTGCTT‑CTCCGCGGGTAAA‑CCCTCC‑TACCGGGCAACTTGTTCCTTCGTGGGAG‑TGGGTTTACCCGCGAGGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTac                                                                                <  2:4472498/137‑1 (MQ=255)
                                                            cGTCACCGCGGTTGATCCGAGCTCGTGCCGGCCT‑TTTCGCGGGTAAC‑CCCACC‑TACCAGGCAACGCTTTCCCTCGTAGGAG‑CGGTTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGt                                                                           <  2:16260801/137‑1 (MQ=38)
                                                                   gcgGTTGATCCGAGTTTGGGCCGGCCT‑CTTCGTGGGGAAA‑CCCGCC‑TACCAGGCAACGCGTTACCTTGTGGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCg                                                                    <  2:13325935/137‑1 (MQ=38)
                                                                      gtggATCTGAGCTTGTGCCGGCCT‑TTTTGTGGGGAAA‑CCCGCC‑GCCCAGGAAACGTGTTACCTCGTGGGAG‑TGGGTTCACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAAt                                                                 <  2:2959602/134‑1 (MQ=37)
                                                                      gtggATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGTAAA‑CCCGCC‑TACCAGGCAACGCGTTCCCTCGTGGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAAt                                                                 <  2:13098359/134‑1 (MQ=255)
                                                                                  ttGTGCCGGCCT‑CTTCGCGGGGAAA‑ACCACC‑TCCCAAGCAACACGCTCCCCCTTAGGAA‑CGGGTTTCCCCACGAAAAAGCCAGCCCCGGTTTAACACCCCCCCCCACTCCACCCTCTTCTCTAACCGCCGttttcgt                                                     >  2:15351306/1‑132 (MQ=255)
                                                                                   tgtgCTGGCCT‑CTTCGCGGGGAAA‑CCCGCC‑TACCGGGCAACGCGTGACTTCGGGGGAG‑CGGTTTGACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTc                                                    <  2:12224906/137‑1 (MQ=255)
                                                                                     tgCTGGCCT‑TTTCGCGGGGAAA‑CCCTCC‑TACCAGGCAACGCTTTACCTTGGAGGAG‑CGGGTTGACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCa                                                  <  1:17487506/137‑1 (MQ=38)
                                                                                     tgCCGTCCT‑CTTCGCGGGGAAA‑CCCGCC‑GACCAGGCAATGTGTTACTTGGGAGGGG‑TGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCa                                                  <  1:12684256/137‑1 (MQ=255)
                                                                                     tgCCGGCCT‑CTTCGCTGGTAAA‑CCCGCC‑TACCAGGCAACGCGTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCa                                                  <  2:6492450/137‑1 (MQ=255)
                                                                                        tggCCT‑TTTTGGGGGGAAA‑CCCGCC‑GACCAGGAAACGTGTTACTTTGGGGGAG‑TGGTTTTACCCGCGAGGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCg                                               <  1:12120865/136‑1 (MQ=255)
                                                                                        cgtccT‑CTTCGCGGGGAAA‑CCCGCC‑GACCGGGAAACGTTTTACCTTGGGGGAG‑CGGGTTTACCCGCGAGGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCg                                               <  2:10686390/134‑1 (MQ=255)
                                                                                             t‑ctTCGGGGGGAAA‑CCTGCC‑GACCGGGCGCAGCGTTACCTTTGAGGGG‑TGGGTTTACCTGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTATCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                          <  2:6010876/137‑1 (MQ=255)
                                                                                             t‑ctTCGGGGGGAAA‑CCCGCC‑TACCAGAAAACGTGTTACCTTGTAGGAG‑TGGGTTTACCCGAGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                          <  1:17320271/137‑1 (MQ=255)
                                                                                             t‑ctTCGGGGAGAAA‑CCCGCC‑TACAAGGCAACGTGTTCCTTCGGAGGAG‑CGGTTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                          <  1:12454910/137‑1 (MQ=38)
                                                                                             t‑ctTCGCGGGGAAA‑CCCTCC‑TACCAGGCAACGCGTTACCTCGGAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                          <  2:16948530/137‑1 (MQ=255)
                                                                                             t‑ctTCGCGGGGAAA‑CCCTCC‑TACCAGGCAACGCGTCACCTCTGAGGAG‑CGGGTTCACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                          <  2:761161/137‑1 (MQ=38)
                                                                                             t‑ctTCGCGGGGAAA‑CCCTCC‑TACCAGCCAACGCGTTACCTTGTGGGAG‑TGGTTTCACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCATCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                          <  2:8768301/137‑1 (MQ=255)
                                                                                             t‑ctTCGCGGGCAAA‑CCCGCC‑TACCAGGCAACGCGTTACCTCGGAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                          <  2:1811489/137‑1 (MQ=255)
                                                                                              ttttagcgGGAAAC‑CCCACC‑TACCAGAAAACTCTTTACCTCGGGGGAG‑CGGGTTCCCCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                          <  1:1107248/132‑1 (MQ=255)
                                                                                              ttttCGCGGGCAAA‑CCCGCT‑TACCAGGCAACGTTTTACTTTGTAGGAT‑TGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                          <  1:3722440/135‑1 (MQ=38)
                                                                                              cctTCGCGGGTAAA‑CCCGCC‑CACCATGCAACGCGTCACCTCGTAGGAG‑CGGTTTTACCCGCGAAGAGGCCGGCCCAGTTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                          <  2:7931012/136‑1 (MQ=38)
                                                                                               ttttgcgGTTAAA‑CCCGCC‑TACCGGGAAACGAGTTACCTCGTAGGAG‑CGGGTTTACCCGGGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCtt                                         <  2:13505814/133‑1 (MQ=38)
                                                                                                  cgcgGGGAAA‑CCCACC‑TACAAGAAAACGCGTGACCTCGGAGGAG‑CGGGTTCACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACa                                      <  2:3131965/137‑1 (MQ=38)
                                                                                                  agcgGGGAAA‑CCCGCC‑GACAGGAAAACGTGTCACCTTGGAGGAG‑CGGGTTCACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACa                                      <  2:1502174/136‑1 (MQ=37)
                                                                                                   gaggggAAA‑CCCGCC‑TACCAGGCACCTCGTTCCCTCTTAGGAA‑CGGTTCTACCCCCAAAGAGGCCGGCCCAGGTTGAACACCGCTCCCATCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACaa                                     <  2:14470204/135‑1 (MQ=255)
                                                                                                   gaggggAAA‑CCCGCC‑TACCAGGCAATGCGTTACCTCGCAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCCCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGCCCATCGCCCCCTACaa                                     <  2:12028758/135‑1 (MQ=38)
                                                                                                    tggggAAA‑CCCGCC‑TACGAGGAAACGTTTTACCTTGGAGGGG‑TGGTTTTACCCGCAAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTAGCGAATAGCCGTTGCAGTCCATCGCCCCTTACAag                                    <  1:15072205/136‑1 (MQ=255)
                                                                                                    gggggAAA‑CCCTCCTTACCAGCCAACCTGTGCCCTCGGAGGAG‑CGGGTTAACCCGAGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACaa                                     <  1:2702028/136‑1 (MQ=255)
                                                                                                         aaa‑CCCGCC‑GACAAGGAAATGTGTGACCTCGGGGGAG‑CGGGTTGACCCGTGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAg                               <  2:2104623/137‑1 (MQ=255)
                                                                                                           a‑CCCGCC‑TACCAGGCAACGCGTTACCTCGTCGGAG‑CGGGTTTCCCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGa                             >  1:7823700/1‑137 (MQ=255)
                                                                                                           a‑CCCGCC‑TACCAGGCAACGCGTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTAAACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGa                             >  2:3841703/1‑137 (MQ=255)
                                                                                                                  c‑TACCGGACAACTCTTTACCTTGGAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCAccc                       <  2:15810000/137‑1 (MQ=38)
                                                                                                                    tACCAGGCAACGCGTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCg                      >  1:8725798/1‑137 (MQ=255)
                                                                                                                     aCCAGGCAACGCGTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGc                     >  2:3626723/1‑137 (MQ=255)
                                                                                                                     aCCAGGCAACGCGTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGc                     >  2:6318608/1‑137 (MQ=255)
                                                                                                                      ccAGGCAACGCGTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCCCCCGcc                    >  2:8323699/1‑137 (MQ=255)
                                                                                                                      ccAGGCAACGCGTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGcc                    >  2:515574/1‑137 (MQ=255)
                                                                                                                        aGGCAACGCGTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCAt                  >  1:17144086/1‑137 (MQ=255)
                                                                                                                         ggCAACGCGTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATg                 >  1:7300149/1‑137 (MQ=255)
                                                                                                                            aaCGCGTTACCTCGTAGGCG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTc              >  1:3071446/1‑137 (MQ=255)
                                                                                                                            aaCGCGTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTc              >  2:6869328/1‑137 (MQ=255)
                                                                                                                            aaCGCGTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTc              >  2:12263199/1‑137 (MQ=255)
                                                                                                                            aaCGCGTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAACCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTc              >  1:13206249/1‑137 (MQ=255)
                                                                                                                             aCGCGTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCg             >  1:2501719/1‑137 (MQ=255)
                                                                                                                              cgcgTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGa            >  1:10606774/1‑137 (MQ=255)
                                                                                                                               gctttACCTTGGAGGAG‑CGGGTTGACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATATCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGat           <  2:7155786/134‑1 (MQ=39)
                                                                                                                                cgTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGata          >  2:13716010/1‑137 (MQ=255)
                                                                                                                                cgTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGata          >  1:9433386/1‑137 (MQ=255)
                                                                                                                                 ttgaCCTCGGGGGAG‑CGGTTTTACCCGCGAGGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGatat         <  1:13462754/134‑1 (MQ=255)
                                                                                                                                 gTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGatat         >  2:4662195/1‑137 (MQ=255)
                                                                                                                                 gTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGatat         >  2:7364096/1‑137 (MQ=255)
                                                                                                                                   tACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATcc       >  1:6769/1‑137 (MQ=255)
                                                                                                                                   tACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATcc       >  1:16483483/1‑137 (MQ=255)
                                                                                                                                    aCCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCg      >  1:13958114/1‑137 (MQ=255)
                                                                                                                                      cccGTAGGAG‑TGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCCTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCa    <  2:3993503/135‑1 (MQ=255)
                                                                                                                                      cTCGTGGGAG‑TGGTTTTACCTACGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCa    <  1:6485305/137‑1 (MQ=255)
                                                                                                                                      cTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCa    >  1:561135/1‑137 (MQ=255)
                                                                                                                                      cTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCa    <  2:9099180/137‑1 (MQ=255)
                                                                                                                                      cTCGTAGAAC‑CGTGTCTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCa    <  2:160254/137‑1 (MQ=255)
                                                                                                                                      cTCGGAGGAG‑TGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCa    <  2:17193955/137‑1 (MQ=255)
                                                                                                                                      cTCGGAGGAG‑CGGTTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCa    <  2:544133/137‑1 (MQ=255)
                                                                                                                                      cTCGGAGGAG‑CGGGTTTACCCGCGAAGAGGCCGTCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCa    <  2:2566471/137‑1 (MQ=255)
                                                                                                                                       tCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCaa   >  1:1365493/1‑137 (MQ=255)
                                                                                                                                       tCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCaa   >  1:8653960/1‑137 (MQ=255)
                                                                                                                                       tCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCaa   >  2:3446802/1‑137 (MQ=255)
                                                                                                                                          |                                                                                                                                       
AGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCT‑CTTCGCGGGTAAA‑CCCGCC‑TACCAGGCAACGCGTTACCTCGTAGGAG‑CGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAA  >  AE015451_phaM1/5344351‑5344620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑