| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AE015451_phaM1 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS CDS (Tryptophan‑‑tRNA ligase) → / → zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451_phaM1 | 1,499,497 | 1 | . | C | 77.6% | 467.0 / 195.9 | 216 | intergenic (+74/‑90) | trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Reads supporting (aligned to +/- strand): ref base . (21/27); new base C (130/36); total (151/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.86e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | AE015451_phaM1 | 1,499,498 | 0 | T | G | 85.0% | 440.0 / 27.6 | 216 | intergenic (+75/‑89) | trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Reads supporting (aligned to +/- strand): ref base T (20/8); new base G (119/39); total (151/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 232 total aligned reads displayed.
TGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑CACGGCTTTGCCGGTG‑TT‑T‑CG‑GGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTT > AE015451_phaM1/1499363‑1499633 ||| tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCCCGAAACAAGCGCCGCGGTGCCTTGC‑CCCGGCTCTTCCGGTG‑tt‑tg > 1:11151487/1‑136 (MQ=255) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCCCGAAACAAGAGCCACTGTGCACGGC‑ACCAGACTTTTCGGTG‑TT‑TGCG‑GGAAc‑g‑cc > 1:10365934/1‑137 (MQ=255) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACAAAACAAGCGCCGCTGTGCATGGC‑ACCAGCTTTTCCCGTG‑TTCG‑CG‑GGCAc‑g‑cc > 1:14698481/1‑137 (MQ=25) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGTCCACGAAACAAGCGCCACGGTGCCTTGCACCCGCCTTTCCCGTTT‑TC‑T‑CG‑GGcccacc > 1:7926179/1‑132 (MQ=255) tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCATGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑TTCG‑CG‑GGCAc‑g‑cccg > 1:13546675/1‑135 (MQ=25) tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGTCCGCGAAACAGGCGCCGCGGTGCCTGGC‑ACCGGCTTTGCCGGTG‑TTCG‑CG‑GGCAc‑g‑ccca > 1:18477335/1‑135 (MQ=37) ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCGCGAAAAAGGCGCCGCGGTGCATGGC‑AACGGCTTTGCCGGTG‑TTCG‑CG‑GGCACAC‑CCGCTccc > 2:506186/1‑137 (MQ=25) agggCCTGAACGAAGCCTTGGCACCACAACATGATTGAGGCTGCCAAACGGTGGGAGCGGGTGGGCCCGCGAAACGGGCGCCGCGGTGCATGGC‑ACTGGTTTTGCCGGTG‑TTCG‑TG‑GGAAC‑GCCCGCTCCCACAGAc < 2:12209045/134‑1 (MQ=255) gagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAAAAGGCGCCGCGGTGCATGGC‑AACGGCTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACt > 2:12727240/1‑137 (MQ=25) gagCCTGAACGAAGCCTTGGCACCACAACAGGATTGAGGCTGCCAAATGGTGGGAGCGGGCGTGCCCGAGAAACGGGCGCCGCGGTGCATGGC‑ACTGGTTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACt < 2:11572861/137‑1 (MQ=18) aagCCTGAACAAAACCTTGGCACCAAGGAAGGACGGAGGCTGCAAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGACACCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑TTCG‑CG‑GACAC‑GCCCGCTCCCACAGACt < 2:17107008/136‑1 (MQ=9) cGAAGCTTTGTCACCACAGCAGGGTTGAGGCTACCAAACGGTGGGGGCGGGCGTGCGCGCGAAACGGGCGCCGTTGTGCGTGGC‑ACCGGCTTTGCTGGTG‑TT‑TGCG‑GGCAT‑GCCCGCTCCCACAGACTGATTGCCAt < 2:15808892/137‑1 (MQ=16) cGAAGCATTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAATGGATGTCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑TTCG‑CG‑GGCGC‑GCCCGCTCCCACGGACTGATTGCCAt > 2:10516894/1‑137 (MQ=18) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCACGGTTCATGGC‑ACCAGCTTTGCCGGTG‑TTCT‑CA‑GGTAC‑ACCCCCTTCCACAGACTGATTGGCAtt > 1:13167018/1‑137 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGC‑ACCAGCTTTGCCGGTG‑TTCT‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCAtt > 2:16580361/1‑137 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑TTCT‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCAtt > 2:15614547/1‑137 (MQ=25) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACCGGCGCCGCGGTGCATGGC‑ACCGGATTTGCCGGTG‑TT‑TGCA‑GGGAC‑GCCCGCTCCCACAGACTGAGTGGCagt > 2:7477864/1‑135 (MQ=18) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCAAAAAAAGAACCTCAGTGCATGGC‑ACCAGCTTTTCCAGTGTTT‑T‑CG‑GGCACAC‑CCCCTCCCACCAACCTATTGcccct > 2:5433856/1‑134 (MQ=255) aaaGCTTTGGCACCACAGCAGGATGGGGGCTGCAAAATGGTGGGAGGGGTCGGCCCTGCAAAACGGGCGCCGTGGGGAATGGC‑ACTGGTTTTGCTGGTG‑TTCG‑TG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCAtt < 1:12792947/136‑1 (MQ=12) cattGGCACAAAAGCAGGAATGAGGCTGCAAAACGGTGGGAGCGGGCATGCCCGCGAAACAGGCGCCGCGGTGCATGGA‑CCCGGGTTTGACGGTG‑TTCT‑CG‑GGCAC‑GCCCAGTCCCACAGACCTATTGCCATTAAgt > 2:12078630/3‑136 (MQ=16) ttGACACCACAGAAGAACTGAGGCTGCCAAACGGTGGGAGCGGGCTTTCCTAAGAAAAAGTCGCCGTGGGACAGGAC‑ACCGGTTTTCCCGGTG‑TT‑CGCG‑GACAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 2:77658/137‑1 (MQ=255) gCACCACGGCGGGATTGAGGTGACCAAATGGTGGGAGGGGTTGTGCCCGCGAAACAGGAGCCAAGTGGCATGGA‑ACTGGTTTTTCCGGTT‑TTCG‑CG‑GGCAC‑ACCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 2:14700943/137‑1 (MQ=255) gCACCACAGCAGGGCTGAGGCTGCCAAACGTTGGGAGCGGGCGGCCCCGCGAAACAGGCCCCAAGGGGCAGGGC‑ACTGGCTTTGCCGGTG‑TT‑TGCG‑GGCAC‑ACCCGCTCCCGCAGACTGATTGCCATTAAGCGGGcc < 2:3931265/137‑1 (MQ=255) gCACCACAGCAGGACTGAGTCTCCCAAACGGTGGGAGCGGGTGTGCCCGCGAAACGGGCGCCGCGGTGCAGGAA‑ACCGGCTTGGCCGTTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 2:2851360/137‑1 (MQ=18) gCACCACAGCAGGACGGAGGCTGCCAAATGGTGGGAGCGGGTGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGTTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 2:8857660/137‑1 (MQ=21) acaccacATCAGGACTGAGGCGGCAAAATGGGGGGAGCGGGTGTGCCAGAGAAACAGGCACAGCGGGACATGGC‑ACCGGTTTTGCCGGTT‑TTCG‑CG‑GGAAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 2:9717769/136‑1 (MQ=255) caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGC‑ACCGGCTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGAATGATTGCCATTAAGCGGGCCt > 2:15651896/1‑137 (MQ=21) gcagGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAAGCACCACGGTGCCCGGC‑ACCCGCTTTTCCGGTG‑TTCT‑CG‑GGCACAC‑CCCCCCCCCCAAAATGATTGCCACTCCACGGGACTATCACtc > 1:11976457/1‑136 (MQ=255) gAGGCTGCAAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGGGCATGAC‑ACCGGCTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACg < 2:16166847/137‑1 (MQ=18) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑TT‑TGCG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCCTTCAGCGGGCCTATCGCTACAGTGACGGccc > 2:7075035/1‑137 (MQ=21) aaaCGGTGGGAGCGGGCGTTCCCGCGAAACAGGCGCCACAGTGCACGGC‑ACCGGCTTTGCCTGTG‑TTCA‑CG‑GGCAC‑GCCCGCCCCCCCCGACTGATTGCCCTTCAACAGGCCTATCGCTACAGTGACGGCCCGtttt > 2:8547585/1‑137 (MQ=255) aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑TTCG‑CG‑GGCACAC‑CCGCTCCCACAGACCGATTGCCATTAAGCGGGCCTATCGCTAAAATGACGGCCCGTTTTTTGTTGCCTc > 2:18046116/1‑137 (MQ=18) gCGGGCGTGCCCACGAAACAAGCGCCGCGGTGCACGGC‑ACCGGATTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAAACTGATTGCCATTCAGCGGGCCTACCGCTACAGTGACGGCCCGTTTTTTTTTGCCTCg > 2:4021771/1‑137 (MQ=18) cGGGCATTCCCGCAAAACAGGCGCCGCGGCGCATGGC‑ACCTACTTTACCGGTG‑TTCG‑CG‑GGCACTC‑CCACTCCCACAGACTGATTACCATTAAACGGGCCTATCGCTACACTGACGGCCCGTTTTTTGTTGCCTCGc < 2:15756961/137‑1 (MQ=255) gCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCACGTTTTTTGTTGCCTCGCTAACGaa > 1:18377949/1‑137 (MQ=25) ttcgAAACAGGCGCTACGGGGCATGGC‑ACTGGCTTTGCTGGTG‑TT‑CGCG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:17489204/135‑1 (MQ=25) cgtgAAACAGGCGCCGAGGTGCCTTGC‑ACCGGCTGTGCTGGTG‑TT‑C‑GGTGGCAC‑GCCCGCTCTCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:4228083/134‑1 (MQ=255) cgcgAAATGGGCACCGCGGTGCATGGC‑ACCGATTTTCCCGGTG‑TTCG‑GG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:2797099/137‑1 (MQ=25) cgcgAAACGGGTGCCGTGGGAGATGGC‑ACCGGTTTGGTTGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:1644419/137‑1 (MQ=21) cgcgAAACAGCCCCCGTGGGGAATGGC‑ACTGGCTTTGCTGGTG‑TTCA‑GG‑GGCAT‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:8479008/137‑1 (MQ=255) cgaaaaaCAGGAGCTGTGGTGCGTGGC‑ACCGGTTTTGCCGGTG‑TT‑CGTG‑GGCAC‑ACCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:982101/133‑1 (MQ=255) gcgAAACAGGCGCCGTGTTCCGTGTC‑ACTGGCTTGGCTGTGG‑TT‑CGCG‑GGCAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGCGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:14250331/137‑1 (MQ=255) gcgAAACAGGCGCCACGGTGCATGGC‑ACCGGTTTTGCTGGTG‑TT‑CGCG‑GACAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:8053469/137‑1 (MQ=255) acaaaaCAGGCGCCCCGATGCAGGGC‑ACCGCCTTTGCAGGTG‑TCCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTTACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:3343609/134‑1 (MQ=25) gAAAAAGGCGCCGCGGTACAGGGC‑ACTGGTTTTTCTGTTG‑TTCG‑CG‑GACAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 2:8738887/137‑1 (MQ=255) aCAGGCGCCGCGGTGCATGGC‑ACCGGGTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAAACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGAATc > 2:9215777/1‑137 (MQ=25) aCAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc > 1:3223687/1‑137 (MQ=37) cgctgtggTGCGTGGC‑ACCGGCTTTGCCGTTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 1:13832102/131‑1 (MQ=25) cgccgtGGTGCATGGC‑ACCGGTTTGGCTGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:7873477/137‑1 (MQ=37) cgccgcGGTGCATGGC‑ACCGTCTTTGCTGGTG‑TTCG‑CG‑GACAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:17925980/137‑1 (MQ=255) cgcgGTGCATGGC‑ACCGGCTTTGCCGGTG‑TT‑CGGG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa < 2:17178491/137‑1 (MQ=37) cATGGC‑ACCGGCTTTGTCGGTG‑TTAG‑CG‑GGCAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 1:6980392/137‑1 (MQ=255) cATGGC‑ACCGGCTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 2:13012918/137‑1 (MQ=37) ggcaccGGCTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 2:5953570/6‑137 (MQ=37) ggcaccGGCTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 1:10193723/6‑137 (MQ=37) accGGCTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAg > 1:9082585/3‑137 (MQ=38) accGGCTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAg > 1:10824106/3‑137 (MQ=38) cGGCTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt > 1:14354154/1‑137 (MQ=38) cGGCTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt > 1:8282516/1‑137 (MQ=38) cGGCTTTGCCGGTG‑TT‑CGCG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCATATCGCTACAGTGACGGCCAGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt > 2:16763282/1‑137 (MQ=38) cTTTGTCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:10904530/1‑137 (MQ=255) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCCCAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTCGAACGCTATCAAGCAGATCTg > 2:17811087/1‑137 (MQ=38) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa > 1:14493433/1‑105 (MQ=37) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:14493433/105‑1 (MQ=37) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa > 2:3397235/1‑116 (MQ=38) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 1:3397235/116‑1 (MQ=38) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:2753269/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:11385035/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:11624348/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:6853634/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:16009809/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:3791724/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:18014491/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:15655455/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:12581058/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:17550587/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:9341786/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:1620795/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:9324748/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:163945/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCACTTAGAACGCTATCAAGCAGATCTg > 2:10725640/1‑137 (MQ=38) cTTTGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCAACAGACTGATTGCCATTACGCGGGCCTATCGATACAGTGACGACCCGTTTTTTGTTGCCTCGCTAACGAATCATGAATCCCTTAGAACGCTATCAAGCATATCTg > 1:2451806/1‑137 (MQ=37) cTTTGCCGGTG‑TT‑CGCG‑GGCAC‑GCCCGCTCCCACAGACTGATCGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCCTGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:4777418/1‑137 (MQ=38) ttGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:12329714/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 1:6604616/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 1:5625481/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:6019695/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:11940172/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:2205429/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:15412386/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 1:17836626/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 1:391340/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 1:1695341/1‑137 (MQ=39) ttGCCGGTG‑TT‑CGCG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:6449007/1‑137 (MQ=39) ttGCCGGTG‑TT‑CGCG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:18322513/1‑137 (MQ=39) gtg‑tT‑TGCG‑GGCAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTcc < 1:3800781/137‑1 (MQ=255) tgtttgcG‑GGCAT‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTccc < 1:18011277/131‑1 (MQ=255) tgttcgcG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTccc < 1:4155272/131‑1 (MQ=255) tcgcG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAc > 1:7600147/4‑137 (MQ=255) cgcG‑GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAct > 2:14138265/3‑137 (MQ=255) ||| TGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑CACGGCTTTGCCGGTG‑TT‑T‑CG‑GGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTT > AE015451_phaM1/1499363‑1499633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |