| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AE015451_phaM1 | 1,499,508 | +C | intergenic (+85/‑79) | trpS CDS (Tryptophan‑‑tRNA ligase) → / → zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451_phaM1 | 1,499,506 | 1 | . | C | 92.8% | 903.6 / 77.2 | 308 | intergenic (+83/‑81) | trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Reads supporting (aligned to +/- strand): ref base . (18/4); new base C (171/111); total (193/115) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.62e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 325 total aligned reads displayed.
GATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTG‑TT‑T‑CGGG‑CAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTC > AE015451_phaM1/1499373‑1499637 | gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAACAAGAGCCGCGGTGCATGGCACC‑GGTTTTTCCGGTG‑TT‑TACGGG‑CAc‑g‑cc > 1:6044929/1‑137 (MQ=255)gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTTCCCACTAAACAAGCACCGCATTGCATAGCACC‑GACCTTGCCGGTG‑TTCG‑CGGG‑CAc‑g‑cc > 1:15383535/1‑137 (MQ=25)gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAc‑a‑cc > 2:19005410/1‑137 (MQ=25)gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTACCGGTG‑TTCG‑CGGG‑CAc‑g‑cc > 2:19349402/1‑137 (MQ=25)gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCACGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAc‑a‑cc > 2:13168356/1‑137 (MQ=25)gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAGGCGCCACGGTGCACGGCCCC‑GGCTTTTCCAGTG‑TTCT‑CGGG‑TAc‑g‑cc > 2:2472108/1‑137 (MQ=38)gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGGCCGCGAAAAAAGAGCCACAGTGCCCGGCACC‑GAATTTTCCAGTGTTT‑T‑CGGG‑CAc‑a‑cc > 1:7548722/1‑137 (MQ=255) aTGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCGCGGTGCATGGCCCC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAc‑g‑ccc > 2:3069346/1‑136 (MQ=25) ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCAAAAAAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTccc > 2:13985222/1‑137 (MQ=21) aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGTCTGGCACC‑GGGTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCACTCCcac > 2:1792298/1‑137 (MQ=21) aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGAGGTGCATGGCACC‑GGCTTTGCCAGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCcac > 2:5956804/1‑137 (MQ=21) aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCAAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GTCCGCTCCcac > 2:6933881/1‑137 (MQ=21) agagCCGAAACGAAGCTTTGGCACCACAGCAGGACGAAGGCTGCCAAACGGGGGGAGCGGTTGTACCCCCGAAAAAGGCGCCGCGGGGCATGACACC‑GTCTTTGCTGGGG‑TTCG‑AGGA‑CAC‑ACCCGCTCCCACAGAc < 2:18096541/137‑1 (MQ=255) tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAA‑GCCCGCTCCCACAGACTGATTg > 2:16317676/1‑137 (MQ=18) gAACGACGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAGGCGCCACGGTGCACGGCACC‑GGATTTGCCGGTG‑TTCG‑CGGG‑AAC‑GCCCGCTCCCACAGAATGATTGc > 2:12003050/1‑137 (MQ=21) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCTCTCCCACAGACTGATTGc > 2:12420983/1‑137 (MQ=21) cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCCC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGccct > 2:15877932/1‑135 (MQ=21) cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGGCTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCac > 2:1661530/1‑136 (MQ=21) cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAAACTGATTGCCAt > 2:13814346/1‑137 (MQ=18) cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAAGCGCCACGGTGCCTGGCACC‑GGCTTTGTCGGTG‑TTCT‑CGGG‑CCC‑GCCCGCTCCCACAGACTGATTGCCAt > 2:10207892/1‑137 (MQ=21) gAAGCTTTGGCACCACAGCAGGATTGGGGCGGCAAAACGGTGGGAACGGGCGTTCCCGCGAAGCAGTCGCCGTGGTGCGTGGCACC‑GGCTTTGCTGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCAtt < 2:9231633/137‑1 (MQ=12) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAACAAGAGCCGCAGTGCCTGGCACC‑GGCTTTTCCGGTGTTT‑T‑TGGG‑CAC‑GCCCGCTCCCCCAAAACGAGTGCCact > 1:13031249/1‑135 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAAAAAGCGCCGTGGTGCCCGGCACC‑TGCTTTGCCGGTG‑TT‑TGCGGG‑CAC‑GCCCGCTCCCACCAACCGAGTGCCAtt > 1:17826374/1‑137 (MQ=19) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCGCGAAAAAGGAGCCGCGGTGCATGGTACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGAATGGTTGCCact > 1:8670328/1‑135 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCAtt > 2:9291196/1‑137 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCACGGTGCATGGCCCC‑GGCTTTGCCAGTG‑TTCG‑CAGG‑CAC‑GCCCTCCCCCACAGACCGATTGCCact > 2:5698429/1‑135 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCACGGTGCACGGCACC‑GGCTTTTCCGGTG‑TTCG‑CGGG‑CAC‑GCCCACTCCCACAAACTGATTGCCCtt > 2:16740160/1‑137 (MQ=18) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAAGGTGGGAGCGGGCGTGTCCGCGAAACAGACGCTGAGATGTCTGTCACC‑GGCTTTGCCGGTG‑TTCA‑CGGG‑TAC‑GCCCGCTCCCAAAGACCGATTGCCAtt > 2:10588537/1‑137 (MQ=19) ttGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGTCGCGGTGCATGGCACC‑GGCTTTGCCAGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGAATGATTGCCACTAAGCgg > 2:15292133/1‑137 (MQ=18) ttGGCACCACAGAAGGATTGGGGTGGCAAAATGGTGGGAGTGGGTGTTCCCGAGAAACAGGCGCTG‑GGGGGAGGGGCACGGGTTTTGCTGGTG‑TT‑TGGGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 1:1105792/137‑1 (MQ=255) gCACCACAGCAGGACGGAGGCTGCAAAACGGGGGGAGCGGGTGTGCCCGCGAAACGGGCGCCGTGTTGCGTGGCACC‑GGTTTTTCCGGTG‑TT‑TGCGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 2:8721340/137‑1 (MQ=21) gCACCACAGAAGGATGGAGGCTGCAAAATGGGGGGAGCGGGCGTGCCCGAGAAAAAGGCACAGTGGGGCGGGAAACC‑GGTTTTGCCGGTG‑TT‑C‑GGGGACAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 1:8793533/137‑1 (MQ=255) gCACCAAAGCAGGACTGAGGCTGCCAAACGGTGGGGACGGGCGTGCCCGCGAAACATGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTA‑TTCG‑TGGA‑CAC‑GCCCGCTCCCACAGACTGATTACCATTAAGCGAGcc < 2:18855286/137‑1 (MQ=17) caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAACAGGAGACACGGTGCATGGCACC‑AGCTTTGCCGGTG‑TT‑TGCGGG‑TACAC‑CCTCTCCCCCAGACTGACTGCCCTTCCGCGGGCCt > 1:7326042/1‑137 (MQ=255) caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGTATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGAACGATTGCCATTAAGCGGGCCt > 2:16908586/1‑137 (MQ=18) aaccacAGAAGGACGGAGGCTACAAAATGGGGGGAGCGGGTGTGCCTGCGAAACGGGCGCCGCGGTGCATGGAACC‑GGTTTTGCTGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCt < 2:7794666/136‑1 (MQ=16) acaGCAGGACTGAGGCTACTAAATGGTGGGAGCGGGCGTTCCCTCGAAACAGGCCCCGCGGTACGTGGCACC‑GGCTTTGCCGGTG‑TTCG‑TGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 2:4428047/137‑1 (MQ=18) acaGCAGGACTGAGGCTACCAAACGGTGGGAGTGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGTTTGGCCGGGT‑TT‑AGCGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 2:17594403/137‑1 (MQ=18) acaGCAGGACTAAGGCTGCCAAACGGTGGGAATGGGCGTTCCCGCAAAACATGCGCCGCGGTACATGACACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 2:7616976/137‑1 (MQ=21) acaGCAGAACGGAGGTGGCAAAAGGGGGGGGGTGGGTGTGCCCGAAAAACAGGCACCGGGGGGCATGGCACC‑GGTTTTGCTGGTG‑TC‑CGAGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 1:18358297/137‑1 (MQ=12) agcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCACGGTGCATGGCACC‑GGCCTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCACTCCCACAGACTGAGTGCCATTCAAAGGGACTCTCGCt > 2:3263343/1‑137 (MQ=17) gACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCACGAAAAAAGCGCCGCGGTGCATGGCCCC‑GGCCTTTCCGGTG‑TTCG‑CGGG‑CAC‑ACCCACCCCCACAAACCGATTTCCCTTCAACAGGCCTCTCGCTAccat > 1:9433310/1‑134 (MQ=255) ggAGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGACACC‑GGCTTTGCCGTTG‑TT‑CGCGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc < 2:14410429/136‑1 (MQ=18) cTGCCAAACGGTGGGAGCGGGCGTTCCCGCGAAACAGGGGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CACAC‑CCGATCCCACAGGATGATTGTCATTAAGTGGGCCTATCGCTGAAGTGGCGGtcc > 2:5326698/1‑137 (MQ=9) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCAGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTCAGCGGGCCTATCGCTCCAGTGACGGccc > 2:1500370/1‑137 (MQ=17) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCGTTGCCGGTG‑TTCA‑CGGG‑CAA‑GCCCGCTCCCACAAACTGATTGCCATTCAGCGGGCCTATCGCTCAAGTGACGGccc > 2:9024319/1‑137 (MQ=18) gCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACC‑GGCTTTGCCGGTG‑TT‑CGCGGG‑CAC‑GACCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTCCAGTGACGGCCCGt > 2:18547803/1‑137 (MQ=21) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCAGTGCATGGCCCC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGACTGCCATTAAGAAGGACTATTGCTAAAGTGACGGTCCGtttt > 2:9729314/1‑137 (MQ=17) aaCGGTGGGAGCGGGCGTGTCCGCGGAACAGGCGCCGCGGTGCATGGCACC‑GGATTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGAGGGCCTATCGCTACAATGACGGCCCGctttt > 2:11547479/1‑137 (MQ=18) aaCGGTGGGAGCGGGCGTGTCCACGAAACAGGCGCCGCGGTGCATGGCACC‑GGCCTTGCCGGTG‑TTCG‑CAGG‑CACAC‑CCACTCCCACAAACCGATTGCCACTCAACAGGCCCATCACTCCAGTGACAGCCCAttttt > 2:6589226/1‑137 (MQ=9) gTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TT‑CGCGGG‑CAC‑GCCCGCTCCCACAGACTGATTTCCATTAAGCGGGCCTCTCGCTACAATGACGGCCCGGTTTttgtt > 2:16147671/1‑137 (MQ=18) ggggAGCGGGCGTGCCCGCGAAACAGGCGCCGTGGGGAATGGAACC‑GTCCTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgttg < 2:9127012/136‑1 (MQ=21) ggAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTTCCGGTG‑TTCG‑CGGG‑CAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGcc < 2:9485577/137‑1 (MQ=21) gCGGGTGTGTCCGCGAAATAGGCGCCGCGGTGCATGGAACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGAATGATTGCCATTAAGCGGGACTATCGCTACAGTGACGGCCCGTTTTTTGTTGCATCg > 2:19213887/1‑137 (MQ=21) gCGGGCGTGCCCACGAAAAAAGCGCCACGGTGCATGGCACC‑AGCTTTTCCGGTG‑TTCG‑CGGG‑CAC‑ACCCGCTCCCACAGAATGAGTGCCATTAAGCGGGCCTATCGCTACAATGACGGACCATTTTTTGTTGCCCCg > 2:16086950/1‑137 (MQ=255) tgtttttGCCCGTGAAACAGGCCCCGTGGGGCGCGACACC‑GGCTTTGCCGGTG‑TT‑CGAGGA‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTAGCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc < 2:10692027/131‑1 (MQ=19) cGGGCTTGCCCGCGAAACAGGCGCCGCGGGGCATGACACC‑GGCTTGGCTGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc < 1:14500790/137‑1 (MQ=21) gtgTGCCTTCGAAACGTGCGCCGTGGTGCGTGGCACT‑GTCTTTGCTGGTG‑TT‑CGTGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa < 1:2380521/135‑1 (MQ=19) cgtgAAACAGGCGCTGTGGTGCATGGCACC‑GTCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:14066113/134‑1 (MQ=25) cgcgAAACAGGCGCTACGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑TGGA‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACNGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:18785082/137‑1 (MQ=25) cgcgAAACAGGCGCCGCGGTGCATGGCACT‑GGTTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:14559781/137‑1 (MQ=25) cgcaAAACAGGCGCCACGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑TGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:13625716/137‑1 (MQ=25) cgagAAAAAGGCGTCGTGGTGCAGGGCACT‑GGTTTTGCTGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:16714405/134‑1 (MQ=25) agagAAACAGTCGCCGCGGTGCATGGCACT‑GGCTTTGCAGGTG‑TT‑CGCGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:7872206/134‑1 (MQ=25) tcgAAAAAGGCTCCGCGGTGCGGGGAACC‑GGTTTTTCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:4996916/136‑1 (MQ=25) gggAAACGGTCGCCGTGGTGCATGGCACT‑GTCTTTGCTGTTT‑TTCG‑CGGA‑CAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 1:13475424/135‑1 (MQ=255) gcgAAACAGGCGCCGCGGTGCAGGGCACC‑GGCTTTTCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:11731394/137‑1 (MQ=37) gcaaaaCAGGCCCCGCGGTACAGGGCACC‑GGCTTTCCCGGTT‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:5654137/134‑1 (MQ=25) gagAAACGGGCGCCGTGGGGCGTGGCACC‑GGTTTGGCTGTTG‑TT‑TGGGGG‑CAC‑ACCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 1:5203727/135‑1 (MQ=255) gAAACAGGCGCCGCGGGGCATGGCACC‑GGTTTTGCTGGTG‑TTCG‑TGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 1:17650694/137‑1 (MQ=37) aaCAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa > 1:540212/1‑132 (MQ=37) aaCAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACt > 2:13618278/1‑137 (MQ=37) acggtcgccgcGGTGCATTGCACC‑GGTTTTGCTGGGG‑TTCG‑TGGG‑CAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc < 1:19440212/132‑1 (MQ=255) cgccgtGGTACGTGGCACC‑GGCTTTTCCGGTT‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:13136217/137‑1 (MQ=25) cgccgtGGGGCATGGCACT‑GGTTTTGCTGGTG‑TT‑C‑GGGGACAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 1:1905471/137‑1 (MQ=255) ctgtggTGCGTGGCCCT‑GGTTTTTCCGGTG‑TT‑TGCGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGa < 1:3389339/133‑1 (MQ=255) ctgcgGGGCAGGGCACC‑GGTTTTGCCGGTG‑TTCG‑AGGG‑CAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGa < 1:16125544/135‑1 (MQ=255) ccgcGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGa > 1:10129997/1‑137 (MQ=37) cATGGCACC‑GGCTTTGCCGGTG‑TTCG‑TGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 2:12333394/137‑1 (MQ=37) cATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 2:12693782/137‑1 (MQ=37) cATGGCACC‑GGCTTTGCCGGTG‑TT‑CGCGGG‑CAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 2:11666021/137‑1 (MQ=37) ggcacc‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 1:3699995/6‑137 (MQ=37) ggcacc‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 2:11200355/6‑137 (MQ=37) acc‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAg > 2:4722300/3‑137 (MQ=38) acc‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAg > 2:17053346/3‑137 (MQ=38) c‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt > 1:7617038/1‑137 (MQ=38) ggCTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATc > 1:12129885/1‑137 (MQ=38) cTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAATGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:8436614/1‑137 (MQ=38) cTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCGTGACTCCCTTAGGACGCTATCAAGCAGATCTg > 2:14887565/1‑137 (MQ=38) cTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCCAGCAGATCTg > 1:14072510/1‑137 (MQ=38) cTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:1463526/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:14975006/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:12739307/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:1342042/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 1:6894022/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 1:18545355/1‑137 (MQ=39) tcgcGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAc > 2:1711713/4‑137 (MQ=255) tcgcGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAc > 1:10605428/4‑137 (MQ=255) cgcGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAct > 2:2107025/3‑137 (MQ=255) ggg‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtct > 1:3929480/1‑137 (MQ=255) | GATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTG‑TT‑T‑CGGG‑CAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTC > AE015451_phaM1/1499373‑1499637 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |