BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	278,744	+A	intergenic (‑239/+75)	PP_0224 CDS (Monooxygenase_ DszC family) ← / ← sctC CDS (sulfur compound ABC transporter ‑ ATP‑binding subunit)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	278,742	1	.	A	95.9%	2750.2 / 103.2	847	intergenic (‑237/+77)	PP_0224 CDS (Monooxygenase_ DszC family)/sctC CDS (sulfur compound ABC transporter ‑ ATP‑binding subunit)	–/–
Reads supporting (aligned to +/- strand): ref base . (6/28); new base A (401/387); total (432/415)
Fisher's exact test for biased strand distribution p-value = 1.48e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 980 total aligned reads displayed.

GCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑AACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTC  >  AE015451_phaM1/278606‑278872

  tGATCGCGACAGACGTGTGCTGTTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aa                                                                                                                                  <  1:13332125/137‑1 (MQ=255)

  tGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aa                                                                                                                                  <  1:12513979/137‑1 (MQ=255)

  tGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aa                                                                                                                                  <  2:11209571/137‑1 (MQ=255)

  tGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aa                                                                                                                                  <  2:17067784/137‑1 (MQ=255)

    aTCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaac                                                                                                                                <  1:5374266/137‑3 (MQ=255)

    aTCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaac                                                                                                                                <  2:3756838/137‑3 (MQ=255)

    aTCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaac                                                                                                                                <  2:14123059/137‑3 (MQ=255)

    aTCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaac                                                                                                                                <  1:4608974/137‑3 (MQ=255)

    aTCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCGAATGAGTTATAAAATTTTTATGAACTAACTTTTGGGAATATTTATAGAAGGGGGATTTGTTACAGAGTTGCCGGGCTCTTCG‑C‑GGGT‑aaac                                                                                                                                <  2:17821300/137‑3 (MQ=255)

     tCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaacc                                                                                                                               <  2:14273902/137‑4 (MQ=255)

       gcgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaacccg                                                                                                                             >  1:7181928/1‑132 (MQ=255)

       gcgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaacccg                                                                                                                             >  1:19219546/1‑132 (MQ=255)

       gcgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaacccg                                                                                                                             >  1:17302108/1‑132 (MQ=255)

       gcgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaacccg                                                                                                                             >  2:13940625/1‑132 (MQ=255)

       gcgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aa                                                                                                                                  <  1:14736028/132‑1 (MQ=255)

                gtgtGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTacac                                                                                                                     <  2:3565110/137‑1 (MQ=255)

                gtgtGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTacac                                                                                                                     <  2:5639749/137‑1 (MQ=255)

                 tgtgCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTacaca                                                                                                                    <  1:6600857/137‑1 (MQ=255)

                 tgtgCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGATGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTacaca                                                                                                                    <  2:10906160/137‑1 (MQ=255)

                      tggtccggtggtccggTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGc                                                                                                               >  1:12143988/1‑137 (MQ=255)

                        gtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCt                                                                                                             <  1:8756419/137‑1 (MQ=255)

                        gtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCt                                                                                                             <  2:16767130/137‑1 (MQ=255)

                           cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACCCACATGCCTCaa                                                                                                          >  1:10905050/1‑137 (MQ=255)

                           cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCaa                                                                                                          <  1:3007811/137‑1 (MQ=255)

                           cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCaa                                                                                                          <  1:3355771/137‑1 (MQ=255)

                           cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCaa                                                                                                          >  2:13812969/1‑137 (MQ=255)

                                gtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  1:18275870/137‑1 (MQ=255)

                                gtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  2:13096505/137‑1 (MQ=255)

                                gtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  2:17416206/137‑1 (MQ=255)

                                gtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  1:8520618/137‑1 (MQ=255)

                                gtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  2:6994000/137‑1 (MQ=255)

                                gtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  2:12691288/137‑1 (MQ=255)

                                gtccggtATATATACCCTAATGATTTATAAAATGTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  1:2004839/137‑1 (MQ=255)

                                gtccggtATATATACCCTAATGATTGATAAAATTTTTATGAACTAACTTTGTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  1:10029049/137‑1 (MQ=255)

                                gtccggtATATATACCCTAACGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  2:6539106/137‑1 (MQ=255)

                                     gtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGAt                                                                                                       >  2:4745729/1‑130 (MQ=255)

                                     gtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCa                                                                                                >  2:2487093/1‑137 (MQ=255)

                                     gtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCa                                                                                                <  1:3749406/137‑1 (MQ=255)

                                     gtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACACGCCTCAAGATCTGTGCa                                                                                                >  2:11517279/1‑137 (MQ=255)

                                          tataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTa                                                                                           <  1:16239658/137‑1 (MQ=255)

                                          tataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTa                                                                                           <  2:1573571/137‑1 (MQ=255)

                                           ataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAg                                                                                          <  1:14175360/137‑1 (MQ=255)

                                             aCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGCATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTTCTACACACATGCCTCAAGGTCTGTGCACTGTAGGa                                                                                        >  1:15895631/1‑137 (MQ=255)

                                             aCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGa                                                                                        >  1:7682109/1‑137 (MQ=255)

                                              cccTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAg                                                                                       >  2:18870818/1‑137 (MQ=255)

                                                  aaTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  1:18801402/119‑1 (MQ=255)

                                                  aaTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCggg                                                                                   >  2:16120067/1‑137 (MQ=255)

                                                  aaTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCggg                                                                                   >  2:11904134/1‑137 (MQ=255)

                                                  aaTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACACGCCTCAAGATCTGTGCACTGTAGGAGCggg                                                                                   >  2:5350224/1‑137 (MQ=255)

                                                  aaTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGATCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCggg                                                                                   >  2:7473703/1‑137 (MQ=255)

                                                   aTGATTTATAAAATTTTTATGAACTAACTTTTTGGACTATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGt                                                                                  >  1:2527398/1‑137 (MQ=255)

                                                   aTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTCAACCCGCTCCTACCCCCATGCCTCAAGGTCTGTGCACTGTAGGAGCGGGt                                                                                  >  1:5855183/1‑137 (MQ=255)

                                                   aTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTCCACACATGCCTCCAGATCTGTGCACTGTAGGAGCGGGt                                                                                  >  1:137827/1‑137 (MQ=255)

                                                   aTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTCCACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGt                                                                                  >  2:5063606/1‑137 (MQ=255)

                                                   aTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAAATCTGTGTACTGTAGGAGAGGGt                                                                                  >  1:6861831/1‑137 (MQ=255)

                                                   aTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAAACCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGt                                                                                  >  2:8255005/1‑137 (MQ=255)

                                                   aTGATTTATAAAATTTTTATGAACTAACTTTTTGAAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGGTCTGTGCACTGTAGGAGCGGGt                                                                                  >  2:15339500/1‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTCAACCCGCTCCTACACACATGCCTCAAGATTTTTGCCCTGTAAGAGCGGGttt                                                                                >  1:4007154/1‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGttt                                                                                >  2:714969/1‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGttt                                                                                >  2:2077895/1‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGttt                                                                                >  2:9319279/1‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGttt                                                                                >  2:9729988/1‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGttt                                                                                >  2:5732566/1‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGttt                                                                                >  1:8280988/1‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGttt                                                                                >  1:9049969/1‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCACTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGAGGGttt                                                                                >  1:968746/1‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAAACCGCTCCTCCACACATGCCTCAAGATCTGTGCACTGTAGGAGAGGGttt                                                                                >  1:17832251/1‑137 (MQ=255)

                                                      aTTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACCGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACCCATGCCTCAAGATGTGTGCACTGTAGGAGCAGGtttc                                                                               >  1:18524158/1‑136 (MQ=39)

                                                       tttATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTAc                                                                              >  2:13579257/1‑137 (MQ=255)

                                                            aaaaTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCcgcg                                                                         >  2:18715934/1‑137 (MQ=255)

                                                               cttttcATGAACTACTTTTTTGGAACGTTCAGAGAGGGGGGTTTTGTTACAGAGGTTCCGGTCTCTTCG‑T‑GGGTAAACCCGCTCCTACACACGTTCCCCAAGATTTGGGCATGGTGGGAGCGGGTTTACCCGCGaaa                                                                      <  1:6400885/136‑1 (MQ=255)

                                                                tttttATGAACTAACTTTTTGGAATATTTAGAGAAGGGGGGTTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATCCCTCAAGATCTGTGCACTGGAGGAGCGGGTTTACCCGCGAAAg                                                                     <  2:4487349/137‑1 (MQ=255)

                                                                tttttAGGAACTAACTTTTTGGAATATTTAGAGAAGGGGGATTTGTTACAGGGGTGCCGGGCTCTTCGTG‑GGGA‑AACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGGAGGAGCGGGTTTACCCGCGAAAg                                                                     <  1:15582948/137‑1 (MQ=255)

                                                                                     gAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCgg                                                >  2:3214295/1‑137 (MQ=255)

                                                                                      aaTATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTCCCCGCGAAAGGGCCGGAACAGGTTTACTCGGa                                               >  1:10476000/1‑137 (MQ=255)

                                                                                        tatTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCCTTTCG‑C‑GGGGAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAgg                                             <  2:1041123/137‑1 (MQ=39)

                                                                                        tatTTAGAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAgg                                             <  2:8014937/137‑1 (MQ=255)

                                                                                          tttATAGAAGGGGGATTTGTGACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCCCCTACACACATTCCTCAAGATCTGTGCACTGTAGGAGCGGGTTGACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGcc                                           <  1:14462124/137‑1 (MQ=39)

                                                                                            tataGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTCCCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGc                                         >  2:10026375/1‑137 (MQ=255)

                                                                                            tataGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGc                                         >  2:16492637/1‑137 (MQ=255)

                                                                                                      gggtttGTGACATATGTCCTGGGCTTTTCG‑CGGGGA‑AACCATCTCCTACACACGTTCCCCAAGATCTGTGCACTGTGGGAGCGGTTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAgg                               <  1:4359505/134‑1 (MQ=255)

                                                                                                      ggATTTGTTACAGGGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCTTACACACGTGCCTCAAGATCTGTGCACTGTGGGAGCGGTTTTACTCGCGAAGGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAgg                               <  2:14874054/137‑1 (MQ=255)

                                                                                                       gATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAAATCTGTGCACTGTAGGAGCGGGTTTCCCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGa                              >  1:16994560/1‑137 (MQ=255)

                                                                                                       gATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aa                                                                                                                                  >  2:6013242/1‑36 (MQ=255)

                                                                                                         tttGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCtgt                                                                                                   <  1:4924493/66‑1 (MQ=255)

                                                                                                         tttGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAAc                            >  2:18368299/1‑137 (MQ=255)

                                                                                                           tgtgACAGAGGTTCCGGGCTCTTCG‑C‑GGGTAAACCCGCGCCTACACACATGCCTCAAGATCTGTGCACTGTATGAGCGGTTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACtt                          <  2:12253631/137‑1 (MQ=255)

                                                                                                           tGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACtt                          >  2:18059788/1‑137 (MQ=255)

                                                                                                                    ggTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCCCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCAGAACAGGTTTACTCTGAGGCCGCAGTCCCGAGGAACTTGCGCAAGaa                 >  2:2944291/1‑137 (MQ=39)

                                                                                                                        ccGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTCCCCACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTCCCCGCGAAAAGGCCAGAACAGGTTTACTCGGAGGCCTCCGTCCCGAGGAACTTGCGCAAGAACTGc             >  1:9305947/1‑137 (MQ=255)

                                                                                                                        ccGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTCCACACCTGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTGCCCGCGGAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAATCCCAAGGAAACTGCGCCAGAACTGc             >  1:16681385/1‑137 (MQ=255)

                                                                                                                        ccGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGTCTCAAGATCTGTGCAATGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGc             >  2:8793419/1‑137 (MQ=255)

                                                                                                                         tgggCTTTTTA‑C‑GGGTAAACCCTCCTCTACACACTTGCCTCAAGATTTGTGCACTGTGGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGcc            <  1:11575476/136‑1 (MQ=37)

                                                                                                                         cGGCCTCTTCGTG‑GGGA‑AACCCCCTCCTACACACGTGCCTCAAGATCTGTGCACTGGAGGAGTGGTTTCACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGcc            <  1:15066532/137‑1 (MQ=255)

                                                                                                                          gggCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGGACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCcg           >  2:19344570/1‑137 (MQ=255)

                                                                                                                          gggCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTCCTCGGAGGCCGCAGTCCCGAGGAACTTGAGCAAGAACTGCcg           >  2:8459546/1‑137 (MQ=255)

                                                                                                                             ctctTCG‑C‑GGGTAAACCCGCTCCTCCACACATGCCCCAAGGTCTGTGCAATGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTCCTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGt        >  2:4009112/1‑137 (MQ=38)

                                                                                                                               ctTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTAc      <  2:7709078/137‑1 (MQ=255)

                                                                                                                               ctTCG‑C‑GGGTAAACCCACCCCTACACACATGCCTCAAAATCGGTGCACTGTGGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTAc      <  1:12807472/137‑1 (MQ=38)

                                                                                                                                   g‑c‑gGGTAAACCCGCTCCTCCACACATGCCTCAAGATCTGTGCACTGTCAGAGCGGGTTTACCCGCAAAAGGGCCGGAACAGGTTTCCCCGGAAGCCGCCGTCCCAAGGAACTTGCGCAAGAACCGCCACGTACGctc  >  2:6189662/1‑137 (MQ=255)

GCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑AACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTC  >  AE015451_phaM1/278606‑278872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`