BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	4,344,296	0	A	C	27.4%	298.3 / 27.4	198	intergenic (+27/‑129)	PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)	–/–
Reads supporting (aligned to +/- strand): ref base A (77/66); new base C (54/0); total (131/67)
Fisher's exact test for biased strand distribution p-value = 2.64e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 222 total aligned reads displayed.

TGCCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGC  >  AE015451_phaM1/4344160‑4344424

tGCCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTTCCa                                                                                                                                   >  1:13942668/1‑137 (MQ=255)

tGCCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCa                                                                                                                                   >  2:9183302/1‑137 (MQ=255)

tGCCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCa                                                                                                                                   >  2:9183345/1‑137 (MQ=255)

tGCCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCa                                                                                                                                   <  1:19418829/137‑1 (MQ=255)

tGCCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCa                                                                                                                                   <  1:19418777/137‑1 (MQ=255)

tGCCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCa                                                                                                                                   <  1:1880207/137‑1 (MQ=255)

tGCCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCa                                                                                                                                   <  2:2293375/137‑1 (MQ=255)

tGCCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCa                                                                                                                                   <  2:3816307/137‑1 (MQ=255)

tGCCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCa                                                                                                                                   >  2:12291045/1‑137 (MQ=255)

tGCCGATGCAGCCGGGAGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCAGTAACGCTCTCGTACGTTGCAGCGCCTGCCa                                                                                                                                   <  2:14191996/137‑1 (MQ=255)

    gATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATc                                                                                                                               <  1:3126707/137‑1 (MQ=255)

    gATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATc                                                                                                                               <  1:5049050/137‑1 (MQ=255)

       gCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAg                                                                                                                            <  1:13178749/137‑1 (MQ=255)

         aGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCCTCCCGATTGAGcc                                                                                                                          >  1:15526556/1‑136 (MQ=255)

         aGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCa                                                                                                                          >  2:7700869/1‑137 (MQ=255)

          gCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTTCCAGATCGAGCAc                                                                                                                         >  1:14206459/1‑137 (MQ=255)

          gCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAc                                                                                                                         <  2:15904565/137‑1 (MQ=255)

          gCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCTCCAAACCCAGCAc                                                                                                                         >  2:5898010/1‑137 (MQ=255)

          gCCGGGCGACAGAGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCTAGATCGAGCAc                                                                                                                         >  1:3006027/1‑137 (MQ=255)

             gggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgc                                                                                                                      <  1:16862567/137‑1 (MQ=255)

              ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCTTCCCGATTCAGCaccacc                                                                                                                     >  2:7946818/1‑137 (MQ=255)

              ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAAATCGAGCAccgcc                                                                                                                     >  2:4371932/1‑137 (MQ=255)

              ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGACCGCCCGACCGAGCaccacc                                                                                                                     >  1:5914211/1‑137 (MQ=255)

               gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                    <  2:13235527/137‑1 (MQ=255)

               gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                    <  1:9568045/137‑1 (MQ=255)

               gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                    <  1:1054189/137‑1 (MQ=255)

               gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                    <  2:8536326/137‑1 (MQ=255)

               gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                    <  2:15593784/137‑1 (MQ=255)

               gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                    <  2:18723651/137‑1 (MQ=255)

               gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                    <  2:7436795/137‑1 (MQ=255)

               gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                    <  2:3767023/137‑1 (MQ=255)

               gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                    <  2:4710594/137‑1 (MQ=255)

               gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                    <  1:10943596/137‑1 (MQ=255)

               gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                    <  1:12465570/137‑1 (MQ=255)

               gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCAGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAcagccc                                                                                                                    >  2:13055272/1‑137 (MQ=255)

                cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgcccg                                                                                                                   <  1:4448396/137‑1 (MQ=255)

                cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGACCGAGCAccgcccg                                                                                                                   <  2:16332539/137‑1 (MQ=255)

                 gACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgcacgc                                                                                                                  >  2:14397709/1‑137 (MQ=255)

                    aGCGTGAAGGTGTGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                               <  1:1282488/137‑1 (MQ=255)

                    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                               <  2:3642540/137‑1 (MQ=255)

                    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                               <  2:14596591/137‑1 (MQ=255)

                    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                               <  1:8022508/137‑1 (MQ=255)

                    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                               <  1:6339241/137‑1 (MQ=255)

                      cGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGc                                                                                                             <  1:7700869/137‑1 (MQ=255)

                        tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGcac                                                                                                           <  1:10217772/137‑1 (MQ=255)

                        tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGcac                                                                                                           <  2:18152303/137‑1 (MQ=255)

                        tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGACCCAACACCACCCCCGCGGccc                                                                                                           >  1:8878601/1‑135 (MQ=255)

                        tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAAATCGAGCACCACCcacgcagaac                                                                                                           >  2:1094280/1‑132 (MQ=255)

                          aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                         <  1:7394789/137‑1 (MQ=255)

                           aGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTTCCCGATCGAGAACCGCCCACCCGGCccaca                                                                                                        >  1:16178156/1‑136 (MQ=255)

                           aGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGACTGCCAGATCTAACACCCCCCACGCTGTacaca                                                                                                        >  1:2255287/1‑136 (MQ=255)

                               gCGTTGGGCAGTGGAAGACTCGTGCGTTTATACAGAAGGGGCGGAGAGCGACCGGAGCAATTCGGCTGGGGCGCGTTAACTCTCTTGTACGTTGCAGCGCCTGCCAGATCGATCCCCGCCCGCGCGGCACACGA‑TCt                                                                                                   <  2:17560142/137‑1 (MQ=255)

                                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGA‑TCTGGCa                                                                                               >  2:13676142/1‑137 (MQ=255)

                                    gggCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCCATCGAGCACCACCCGCGCGGAACACAA‑TCTGGTAg                                                                                              >  1:15938601/1‑137 (MQ=255)

                                     ggCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGTGGCTGTTAAGTTGAGGACCACCCGCGCAGGGCAAAA‑TCTTGCAgg                                                                                             >  1:11552614/1‑137 (MQ=255)

                                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCTGCCCGATCGAACACCTCCCCCGCGGCACAAAA‑TCTGGCAGGCGCTGTAc                                                                                     >  1:17476134/1‑137 (MQ=255)

                                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCCGACCGAAACACACCCACGCAGCACACAA‑CCTGGCAGgcgatacaa                                                                                     >  1:3338531/1‑131 (MQ=255)

                                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCCGATCGCGCACCACCCGCGCGGCACACAA‑TCTTGCAAGAGATGCAc                                                                                     >  2:15476134/1‑137 (MQ=255)

                                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCAATCGCGCCCCGCCCGAGCGGTACCAGA‑TCTGGTAGGAGTTGCAc                                                                                     >  2:16425010/1‑137 (MQ=255)

                                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                    <  1:18544173/107‑1 (MQ=255)

                                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCCGACCGAACACCACCCGCGCGGCACACGA‑CCTGGCAGGAGATGCAc                                                                                     >  1:18072089/1‑137 (MQ=255)

                                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCCAATCGAACACCGCCCGCGCGGCACACGA‑TCTGGCAGGCGCTGccc                                                                                     >  1:18754832/1‑135 (MQ=255)

                                                  gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGTCAGATCGAGCACCGCCCACGCGGCACACAA‑TTTGGACGGCGATGCACTACTg                                                                                >  2:19030720/1‑137 (MQ=255)

                                                  gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACCCTCTTGTACGTTGCAGCGCCTTCCAGACCGAGCACCGCCCACGCGGCACACGA‑TCTGGCAAGCGATGCACTCTTg                                                                                >  2:18478945/1‑137 (MQ=255)

                                                     tGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCAGATCGAGCACCACCCGCGCGGCACACAA‑TCTGGCAGGCGCTGCACTATTGGAg                                                                             >  2:19454115/1‑137 (MQ=255)

                                                       cATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTTTCCAACCGAGCACCGCCCACGCGGAACACGA‑TCTGGTGGGCGCTGTACTATTGGAggg                                                                           >  1:8745101/1‑136 (MQ=255)

                                                       cATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAACACCACCCGCGCGGCACACGA‑CCTGGCAGGCGCTGCACTCTTTGAGGc                                                                           >  2:12069365/1‑137 (MQ=255)

                                                       cATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTACCAGATCGAGCACCGCCCGCGCGGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGc                                                                           <  1:19179795/137‑1 (MQ=255)

                                                         tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATTGAGGACCCCCCCTACGGCACACGA‑GCCGGGCGGAGCTGTAATATTTGAGgtgt                                                                         >  2:14714352/1‑137 (MQ=255)

                                                         tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCCGAGCGAGTCCCACCCACGTGGGACAAGA‑CCTGGTCGGCGATTCACCACTTGAGGCGt                                                                         >  2:7192631/1‑137 (MQ=255)

                                                               ccGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCCAATCGAAAACCGCCCACGCAGAACACAA‑TCTGGCAGGCGCTGCACCCTTGGAGGCGTACGccc                                                                   >  2:6712724/1‑137 (MQ=255)

                                                               ccGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGACTGGCAGAGCGAACACCACCCCCACAGCAAACAA‑ACCGGCAGGCACCTCACCACTTGAGGCGTACAccc                                                                   >  1:9797564/1‑137 (MQ=255)

                                                                 gAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATc                                                                                                                               <  2:10759096/76‑1 (MQ=255)

                                                                 gAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGAGTCCGCCCCg                                                                 >  2:4243664/1‑137 (MQ=255)

                                                                   aTGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCAATCGAGCACCACCCACGCGGAACCCAA‑TCTTGCAAGTGCTTCAATCTTTGAGGTGTACACCCCCTc                                                               >  2:17218553/1‑137 (MQ=255)

                                                                    tGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAAATCCAGCACCCCCCACACGGCACACAA‑TCTGGCAGGAGCGGCACCATTGGAGGAGGACGCACCATca                                                              >  1:17580236/1‑137 (MQ=255)

                                                                    tGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAAAGCGAGCCCCGACCACGCGGCACAATA‑TCCGGCAGGCAATGCAACACTGGAGGCGTACGccccatga                                                              >  2:10059403/1‑133 (MQ=255)

                                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCTGCCAGATCGAGGCCCGCCCACGCGGCACACAA‑TCTTGTAAACGCTGCACCATTTGAGGAGTCCACCCCGTcca                                                             >  2:4475544/1‑135 (MQ=255)

                                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCCACCCGACCGAAAACCACCCCCAAGGAACACAACTCT‑GTAGGAGATTCACTATTGGGGGGGTACGCCCCATcac                                                             >  1:12253824/1‑137 (MQ=255)

                                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGTCAAATCGAGCACCACCCCCAAGGAAAACAC‑CCCGGAAAGCGATGGAACATTGGAGGCGTACAcccccccaa                                                             >  1:3259792/1‑132 (MQ=255)

                                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCCAACCGAACACCACCCCCACAGACCACCA‑CCCTGCAGGAGCTGCACCCTTTGAGGAGTACAcccccccca                                                             >  2:8388393/1‑132 (MQ=255)

                                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCCGTCAGATCGAGAACCACCCCCACGGAAAACAA‑GCTTGTCAGAGCTGCACTACTTGAGGAGTCCGCCCCCTcac                                                             >  2:11932595/1‑137 (MQ=255)

                                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCAGATCGAGCACCGCCCACGCGGCACACAA‑TCTGGCAGGCGCTTCACTACTGGAGGCGTACACCCCGTcac                                                             >  2:12584915/1‑137 (MQ=255)

                                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGTCCAATCAAGTAACGCCCACGCGGCACACGA‑CCTTGTAGGCGTTGCCCTATTTGAGGCGTACGCCCCATcac                                                             >  2:10217729/1‑137 (MQ=255)

                                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCCAATCGAACACCCCCCACGCGGTACCCAA‑GCCGGCCGGCGACGTACCATTGGGGGAGTACGCCCCCTccc                                                             >  2:16906667/1‑135 (MQ=255)

                                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGATCTTGTACGTTGCAGCGTCTGCCAGATCGAGCCCCGCCCCCACGGCACAAGA‑GCTTGCAGGAGCTGCAACATTGGAGGCGTACGccccagcaa                                                             >  2:18974254/1‑132 (MQ=255)

                                                                       gCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCTGTCCAATCAAGCACCACCCGCCCAGCACACAA‑TCTTGCAGGCGATTCACCGTTTGAGGAGTACACCCCATcacac                                                           >  2:1163418/1‑136 (MQ=255)

                                                                       gCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAAATCGAGCACCACCCCCACGGCACACAA‑TCCTGCAAGCGCTGCAACCCTTGAAGAGTACGCCCCATcacac                                                           >  2:12997266/1‑136 (MQ=255)

                                                                          gagaGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCAAACCGAACACCGCCCAGACGGCACACGA‑TCCTGAAAGAGATGCACTATTGGAGGAGTACGCCCCATCACACTat                                                        >  2:12246805/1‑137 (MQ=255)

                                                                                 actggagaAAGTCGGCTGGGGAGCATGAACGCCTTTGTACGTTGCGCCGCCTGCAAGATCGAGCACCACCCGCGCGGCACACGA‑TCGGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACg                                                 <  2:4028442/133‑1 (MQ=255)

                                                                                    tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAAAGCGAGCACCGCCCACGTGGCACACAA‑TCTGGCAAGCACTGCACCATTGGAGGCGTACGCCCCGTCACATTCTCCTCCCGCCa                                              >  2:2461384/1‑137 (MQ=255)

                                                                                    tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCATGCCAGATCGAGCACCCCCCGCACGGCAAACGA‑TCTAGAAGGCGCTGCACTCTTGGAGGAATACGCCCCGCCACATCCTCATCACCCCa                                              >  2:12486362/1‑137 (MQ=255)

                                                                                           gTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCCGCCAGATCGAGAAACACCCACGCGGCACAAGA‑TCTGGCAGGTGCTGCACCCTTTGGGGCGTACGCCCCGTCCCATTCTCATCACGCCACTGTCgg                                       >  2:4384972/1‑136 (MQ=255)

                                                                                                   gggCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCAGATCGAGCACCGCCCACGCGGCACACGA‑TCTGGTAGGCGCTGTACTATTGGAGGAGTACACCCCGTCAAACTACCATCACACCACTGTCGTAACCTgtc                               >  2:1353420/1‑135 (MQ=255)

                                                                                                   gggCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCAATCGAGCAACACCCGCGCGGCACACAA‑GCTGGCGGGCATTGGACTATTTGAGGCGTACGCCCAGTAAAAGCATCATCACACCACCGTTGTAAtatccc                               >  2:8923722/1‑132 (MQ=255)

                                                                                                     gcgcATTAACGCTCTTGTACGTTGCAGCGCCTTCCCAATCGAGCACCACCCACGCAGCACACGA‑TCCTGCAGGCGCTGCACTACTGGAGGCATCCGCCCCCTCACATTACCCTCACACCACCTTCGTAATCTGCCgg                             >  2:62393/1‑137 (MQ=255)

                                                                                                            aaCGCTCTTGTACGTTGCAGCGCCTGCCCGATTTAGCACCGCCCACGCGGCACACGA‑TCTGGCAGGTGTTTCACTGTTGGGGGCGTGCTCCCCGTTACATTGTTCTCACGCCACTGTTGTAATCTTTCGGCCAActc                      >  2:18553135/1‑137 (MQ=255)

                                                                                                                        cGTTGCAGCGCCTGCCAGATCGAATACCGCCCGCACGGCACACGA‑TCTTGGAGGAGATGCACTACTGGAGGCGTACGCCCCGTTACATTATCCTCCAAACACTGTCGTCATCTTCCGGCCAACCCTCTGTCACAGGt          >  2:9699832/1‑137 (MQ=255)

                                                                                                                              aGCGCCCGCCCAATCAAGCACCGCCCACGCGGCACACGA‑GCTGGCAGGCGCTTCACTCTTGGAAGCGTACGCCCCGCCACACTATCACCAAGCCACTGTCGTAATCTGCCGGCCAAACCTATGCCACAAGTCAccca    >  2:15557564/1‑136 (MQ=255)

                                                                                                                                cgcCTGCCAGATCTAACACCGCCCACGCGGCACACGA‑TCTTGCCAGCTCTTCCCCCTTTGAGGCGTACGCCCCGTCCCACTATCCTCACACCACTGTCGTAATCTGCCGGCCAACTCTCTGTCACAGGGCACCCGGc  >  2:12877362/1‑137 (MQ=255)

TGCCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGC  >  AE015451_phaM1/4344160‑4344424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`